#LimbIschemia
November 8, 2025 at 11:15 PM
📢 Online First!
Pasqui and colleagues share data to suggest that active malignancy increases mortality but does not affect limb salvage or reocclusion in patients with acute limb ischemia.
Read more 👉 journals.sagepub.com/doi/10.1177/...
#LimbIschemia #PeripheralArteryDisease #VascSky
July 24, 2025 at 9:55 PM
(Mouse/Human) Mitochondria transplantation i.m. improves reperfusion #Angiogenesis of 🐭#LimbIschemia

Macrophage, instead of #EndothelialCell, is the major cell type that internalizes exogenous Mitochondria (sustained by day 3 not 7)

#BioactiveMat 2025
www.sciencedirect.com/science/arti...
July 22, 2025 at 6:53 PM
#SGLT2i #Diabetes #PeripheralArteryDisease

Empagliflozin ⏫#Angiogenesis #Arteriogenesis in diabetic db/db🐭 #Limbischemia

A possible SETD2 (H3K36 methyltransferase)-antioxidant cascade

SETD2i EPZ-719 ⏬the benefits of EMPA in stressed HUVEC

#CardiovascToxicol 2025
www.gimopen.org/article/S294...
July 19, 2025 at 7:09 PM
#Cuproptosis in #ChronicLimbThreateningIschemia

Bulk RNAseq re-analysis ➡️
12 Cuproptotic genes⏬in👤CLTI Gastroc muscle

Copper chelator Ammonium Tetrathiomolybdate▶️
⏬Muscle protein lipoylation
⏫Limb reperfusion
in diabetic🐭 #LimbIschemia

#FreeRadicBiolMed 2025
www.sciencedirect.com/science/arti...
April 23, 2025 at 12:20 PM
Fibroblast-converted #SmoothMuscleCell to treat🐭#LimbIschemia
retroviral Myocardin+all-trans #RetinoicAcid

👉Survive up to 28 d in ischemic limb muscle
👉Assume peri-arterial or peri-capillary locations
👉Pro-angiogenic secretome

#Circ 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/full/10....
March 12, 2025 at 7:32 PM
#PeripheralArteryDisease

#HyperbaricOxygenTherapy (100% O2, 3 ATA, 1 h/d for 5 d⬅️what's the control treatment?🤔) for mouse #LimbIschemia

Activate SIRT1➡️improve mitochondria fusion & OXPHOS in angiogenic #EndothelialCell (how?🧐)

#FreeRadicBiolMed 2025
www.sciencedirect.com/science/arti...
March 2, 2025 at 7:55 PM
Crosslinked Alginate-Collagen #Hydrogel to stabilize & deliver #EndothelialCell (HUVEC)-derived #Extracellular Vesicle, encapsulated with platelet membrane to ischemic🐭limb

Lots of (which) cells express IL-1β in🐭ischemic muscle!😮

#LimbIschemia

#MacroJrnls_ACS 2024
pubs.acs.org/doi/10.1021/...
December 21, 2024 at 1:52 PM
#CircadianRhythm #PeripheralArteryDisease

Perturbed #CircadianClock in #SkeletalMuscle (ACTA-rtTA-TRE-Cre::Bmall) impairs #Myogenesis (⏬myostatin, ⏫myofiber hypertrophy) & #Angiogenesis (Coupled by⏬VEGF-A) in🐭 #LimbIschemia

#ATVB 2024 @ahascience.bsky.social
www.ahajournals.org/doi/abs/10.1...
December 16, 2024 at 1:15 PM
Shaking the flask or Vertical-Wheel Bioreactor of 3D #hiPSC spheroids▶️9-fold⏫#SmoothMuscleCell yield🤠

Therapeutic benefit of Intramuscular injection of hiPSC-SMC in NOD🐭#Limbischemia

Stem Cell Res Ther 2024
stemcellres.biomedcentral.com/articles/10....
December 14, 2024 at 1:05 PM
Oxygen-releasing Perfluorohexane Nanoparticle
Benzaldehyde-polyethylene + hydroxypropyl chitosan self-healing Hydrogel

To deliver🐭 #BoneMarrowMesenchymalStemCell to🐭limb muscle

40% survival at D18

Therapeutic effectiveness in🐭#LimbIschemia

#IntJNanomedicine 2024
www.dovepress.com/an-innovativ...
December 4, 2024 at 7:58 PM
#PeripheralArteryDisease #Netrin

Exosome derived from Netrin 1-overexpressing 🐭#AdiposeDerivedStromalCell⏫
Reperfusion #Angiogenesis 🧐 #Arteriogenesis
Exercise capacity
M2 Mφ polarization
in Diabetic db/db🐭#LimbIschemia

#AdvHealthcMater 2024
onlinelibrary.wiley.com/doi/10.1002/...
December 4, 2024 at 1:48 PM
Bispecific antibody for #LimbIschemia

A dual-targeting antibody T11-RF12 for activating Tie2 & LGR5-FZD10 Wnt signaling in #EndothelialCells

Intramuscular injection (Gracilis) Balb/c🐭
⏫limb reperfusion
Rescue paw necrosis
⏫Angiogenesis Arteriogenesis

mAbs 2024
www.tandfonline.com/doi/10.1080/...
December 3, 2024 at 12:30 PM
Activating #HexosamineBiosynthesisPathway to treat #PeripheralArteryDisease

Glucosamine i.p. ⏫Reperfusion #Angiogenesis but not #Arteriogenesis in🐭#LimbIschemia (BALB/c, eNOS-/- C57BL/6)

Distinct pathways from VEGFA

ATF4 as a signaling mediator

#CircAHA 2024
www.ahajournals.org/doi/10.1161/...
November 14, 2024 at 1:04 PM
What Is the Best Experimental Model for Developing Novel Therapeutics in #PeripheralArteryDisease?

A thoughtful critique on🐭#LimbIschemia models

To improve the translatability
👉Gradual arterial occlusion
👉+ atherosclerotic/thrombotic/embolic conditio

#ATVB 2024
www.ahajournals.org/doi/10.1161/...
October 25, 2024 at 1:22 PM
Mouse #LimbIschemia

Contrast-enhanced ultrasound (whole calf) vs Laser Doppler (primarily skin) imaging of limb reperfusion post-ischemia

C57BL/6J🐭♂️ 10~12 wk

B/w 2- & 4-wk post-ischemia
Laser doppler ⬆️
Ultrasound microbubble ⬇️

Ultrasound Med Biol 2024
www.sciencedirect.com/science/arti...
October 24, 2024 at 7:02 PM
Spatial Atlas of muscular & vascular regeneration in 🐭#LimbIschemia

From our Pickering lab😁
3 positions covering 22 hindlimb muscles

Tight #Angiogeneis-#Myogenesis coupling leaves little border zone in ischemic-regenerating (day 10)🐭muscle

#ATVB 2020
www.ahajournals.org/doi/full/10....
October 18, 2024 at 7:11 PM
#ChronicLimbThreateningIschemia #LimbIschemia

Competing risk analysis to estimate #Amputation incidence & risk in #LowerExtremityPeripheralArteryDisease

7273 case + 5095 ctrl
2 yr f/up

journals.sagepub.com/doi/10.1177/...
September 21, 2024 at 8:04 PM
#EndothelialCell #Mitochodria #LimbIschemia #EndMT

Thrilled to have played a small role in this exciting story😀

Integrated single-cell RNA-seq analysis reveals mitochondrial calcium signaling as a modulator of endothelial-to-mesenchymal transition

#ScienceAdv 2024
www.science.org/doi/10.1126/...
August 12, 2024 at 11:13 AM
Bone in an Artery!😍

A beautiful histopathologic study on #MedialCalcification & #IntimalCalcification Lower Limb Arteries

> 2k sections, 77 (femoropopliteal/anterior tibial/posterior tibial/peroneal/) arteries, 36 #LimbIschemia👤

Sho Torii lab 2024 #JACCAdv
www.sciencedirect.com/science/arti...
June 30, 2024 at 7:08 PM
🐷bone marrow #MesenchymalStemCell encapsulated in Alginate #Hydrogel as cell therapy in 🐷model of #LimbIschemia Iliac arterial excision

Some MSCs survive up to 4 wk in vivo

Limb use - force-time impulses✅ trotting pattern✅
Hyperemic perfusion✅

#JAHA 2024
www.ahajournals.org/doi/10.1161/...
April 23, 2024 at 6:56 PM
An important paper on Macrophage-#MuscleStemCell crosstalks in regenerative dynamics of ischemic #SkeletalMuscle #CriticalLimbThreateningIschemia

Proinflammatory niche perturbs MuSC proliferation-differentiation in #LimbIschemia

#GenomeMedicine 2023
genomemedicine.biomedcentral.com/articles/10....
March 26, 2024 at 7:09 PM
#Nanoceria-coated #GrapheneOxide microsheet as multicellular (HUVEC+MSC) delivery system to treat 🐭#LimbIschemia

⏬early (72 h) ROS
⏫early HIF1α (in what cell types?)
⏫#Angiogenesis+#Arteriogenesis in ischemic adductor muscle

#Biomaterials 2023
www.sciencedirect.com/science/arti...
March 23, 2024 at 7:06 PM
Condition Tie2+ Monocyte/Mφ to treat #LimbIschemia
siRNA HTATIP2⏫NRP1+Angpt1

Tie2+ Mφ from #ChronicLimbThreateningIschemia👤exhibits ⏬angiogenic+arteriogenic benefit in vitro & in🐭

Alberto Smith, Bijan Modarai labs #JCIInsight 2023
insight.jci.org/articles/vie...
March 15, 2024 at 7:08 PM
AteloCollagen 10%-#HyaluronicAcid 5% microgel as Cell Carrier of👤#AdiposeDerivedStemCell to treat🐭#LimbIschemia

⏫ADSC Survival in ischemic limb up to 28 days
⏫Material transfer (nutrient uptake+secretome release)

Which muscle studied?
#BioactiveMat 2023
www.sciencedirect.com/science/arti...
March 12, 2024 at 7:14 PM