Happy to share the final version of this work out in ACS CS. Inspired by ‘binding-focused’ chemoproteomic methods, we developed a ‘function-focused’ strategy to agnostically identify degradable proteins.
This was a big team effort led by
@inesforrest.bsky.social
and in collaboration with AbbVie.
This was a big team effort led by
@inesforrest.bsky.social
and in collaboration with AbbVie.
Proteome-Wide Discovery of Degradable Proteins Using Bifunctional Molecules
Targeted protein degradation (TPD) is an emergent therapeutic strategy with the potential to circumvent challenges associated with targets unamenable to conventional pharmacological inhibition. Among ...
pubs.acs.org
October 21, 2025 at 3:55 PM
Everybody can reply
9 reposts
1 quotes
37 likes
2 saves
Molecular Dynamics-Guided Design and Chemoproteomic Profiling of Covalent Kinase Activity Probes https://www.biorxiv.org/content/10.1101/2025.10.17.683178v1
October 19, 2025 at 2:45 AM
Everybody can reply
1 likes
Chemoproteomic profiling of 8-oxoguanosine-sensitive RNA-protein interactions https://www.biorxiv.org/content/10.1101/2023.09.01.555928v1
Chemoproteomic profiling of 8-oxoguanosine-sensitive RNA-protein interactions https://www.biorxiv.org/content/10.1101/2023.09.01.555928v1
Cellular nucleic acids are subject to assault by endogenous and exogenous agents that can perturb th
www.biorxiv.org
September 2, 2023 at 4:45 AM
Everybody can reply
IBMB Seminar, Friday July 21st. 10:00 AM Speaker: Keriann Backus. Assistant Professor of
Biological Chemistry and Chemistry and Biochemistry
at the UCLA. Title: "Chemoproteomic approaches to stratify the
subcellular redox proteome" Seminar room: Torre I Room 3
Biological Chemistry and Chemistry and Biochemistry
at the UCLA. Title: "Chemoproteomic approaches to stratify the
subcellular redox proteome" Seminar room: Torre I Room 3
November 18, 2024 at 8:31 AM
Everybody can reply
Chemoproteomic profiling of substrate specificity in gut microbiota-associated bile salt hydrolases by Lin Han, Pamela V. Chang, and colleagues at @CornellChem @Cornell @CornellPathogen @CornellCFI http://dlvr.it/THQTfs
January 17, 2025 at 6:01 PM
Everybody can reply
6 reposts
8 likes
Molecular Dynamics-Guided Design and Chemoproteomic Profiling of Covalent Kinase Activity Probes https://www.biorxiv.org/content/10.1101/2025.10.17.683178v1
October 19, 2025 at 2:46 AM
Everybody can reply
Welcome to todays CANS seminar by Kimberly Kline, Univ. of Geneva, Switzerland and Markus Lakemeyer, University of Jena, Germany
'Pathogenesis of Enterococcal biofilm-associated infection'
14:15-15.00
´Chemoproteomic profiling of microbial interactions´
15.00-15.45
MH Aud 5
'Pathogenesis of Enterococcal biofilm-associated infection'
14:15-15.00
´Chemoproteomic profiling of microbial interactions´
15.00-15.45
MH Aud 5
March 13, 2025 at 8:00 AM
Everybody can reply
1 reposts
1 likes
Chemoproteomic profiling of substrate specificity in gut microbiota-associated bile salt hydrolases https://www.biorxiv.org/content/10.1101/2024.04.01.587558v1
Chemoproteomic profiling of substrate specificity in gut microbiota-associated bile salt hydrolases https://www.biorxiv.org/content/10.1101/2024.04.01.587558v1
The gut microbiome possesses numerous biochemical enzymes that biosynthesize metabolites that impact
www.biorxiv.org
April 2, 2024 at 2:16 AM
Everybody can reply
Do you have bioactive compounds/drug molecules and want to unveil the mode of action? Chemoproteomic profiling is a promising solution to reveal the interaction partners in the cells, so to get a sense of how it works.
May 19, 2025 at 9:37 AM
Everybody can reply
1 reposts
2 likes
Lots more on this project that I cant share quite yet, but special thanks to the Sieber lab at TUM for hosting me in Munich to learn and perform these chemoproteomic experiments! Lipoprotein trafficking is clearly an attractive target for gram(-)’s, especially for beta-lactamase expressing bacteria.
June 29, 2025 at 5:36 PM
Everybody can reply
New paper!
Our chemoproteomic survey of phenylhydroxamic acids identifies the first drug-like inhibitors for nucleotide-binding protein HINT1 and nucleoside kinases NME1-4. On top, we found probes for the HDAC inhibitor off-target MBLAC2. Check out the open-access article! tinyurl.com/yh92vh6b
Our chemoproteomic survey of phenylhydroxamic acids identifies the first drug-like inhibitors for nucleotide-binding protein HINT1 and nucleoside kinases NME1-4. On top, we found probes for the HDAC inhibitor off-target MBLAC2. Check out the open-access article! tinyurl.com/yh92vh6b
sev on X: "Paper alert! We report the first inhibitors for nucleoside kinases NME1-4 and for the nucleotide-binding protein HINT1. On top, we provide probes for MBLAC2, an off-target of every second (!) hydroxamic acid-based HDAC inhibitor! https://t.co/uuRCO78xFz 👇🧵" / X
Paper alert! We report the first inhibitors for nucleoside kinases NME1-4 and for the nucleotide-binding protein HINT1. On top, we provide probes for MBLAC2, an off-target of every second (!) hydroxamic acid-based HDAC inhibitor! https://t.co/uuRCO78xFz 👇🧵
x.com
May 9, 2025 at 11:33 AM
Everybody can reply
4 reposts
6 likes
BE seminar this afternoon with Daniel K. Nomura from UC, Berkeley 'Reimagining Druggability using Chemoproteomic Platforms' be.mit.edu/our-communit...
February 27, 2025 at 1:55 PM
Everybody can reply
2 likes
Interested in covalent probes? Check out our most recent pre-print on chemoproteomic profiling of N-terminal cysteines. Nice overview/summary from lead author @esbensv.bsky.social
In my first #bsky post, I am very happy and proud to share the new(ish) preprint of my post doc project on N-terminal cysteine (NCys) chemoprotomics using the oxSTEF probes!
Preprint at 👉 www.doi.org/10.26434/chemrxiv-2024-4w549
Skeeturial (??) below ⬇️ #ChemSky #ChemBio
Preprint at 👉 www.doi.org/10.26434/chemrxiv-2024-4w549
Skeeturial (??) below ⬇️ #ChemSky #ChemBio
November 20, 2024 at 4:00 PM
Everybody can reply
1 reposts
20 likes
A Chemoproteomic Approach for System-Wide and Site-Specific Uncovering of Functional Protein N-Glycosylation pubs.acs.org/doi/abs...
---
#proteomics #prot-paper
---
#proteomics #prot-paper
July 1, 2025 at 8:40 AM
Everybody can reply
2 likes
Live-Cell Chemoproteomic Profiling Identifies the Uncharacterised Protein YbaA as a Direct Target of Ciprofloxacin in Escherichia coli https://www.biorxiv.org/content/10.1101/2025.10.16.682360v1
October 16, 2025 at 7:17 PM
Everybody can reply
Chemoproteomic profiling of serine hydrolases reveals the dynamic role of lipases in Phaeodactylum tricornutum https://www.biorxiv.org/content/10.1101/2024.03.12.582592v1
Chemoproteomic profiling of serine hydrolases reveals the dynamic role of lipases in Phaeodactylum tricornutum https://www.biorxiv.org/content/10.1101/2024.03.12.582592v1
Phaeodactylum tricornutum is a model oleaginous pennate diatom, widely investigated for the accumula
www.biorxiv.org
March 13, 2024 at 9:46 AM
Everybody can reply
New-preprint alert! We use chemoproteomic profiling to show MYST KAT inhibitors exhibit hierarchical target engagement (KAT6A/B > KAT7 >> KAT8). Reminiscent of Type I kinase inhibitors, the selectivity of these small molecules varies based on concentration.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
May 4, 2025 at 2:22 PM
Everybody can reply
4 reposts
30 likes
Development of Second-Generation Acyl Silane Photoaffinity Probes for Cellular Chemoproteomic Profiling https://www.biorxiv.org/content/10.1101/2025.05.24.655972v1
May 29, 2025 at 12:46 AM
Everybody can reply
1 likes
Protein Tyrosine Amination: Detection, Imaging, and Chemoproteomic Profiling with Synthetic Probes pubs.acs.org/doi/10....
---
#proteomics #prot-paper
---
#proteomics #prot-paper
April 17, 2024 at 8:20 AM
Everybody can reply
@ajhgnews.bsky.social sat with @val-arboleda.bsky.social in the latest "Inside AJHG" to discuss her recently published paper, “Prioritizing disease-associated missense variants with chemoproteomic-detected amino acids.”➡️ www.ashg.org/ajhg/inside-... #ASHG #GeneticsDiscoveries #HumanGenetics
July 3, 2025 at 5:34 PM
Everybody can reply
1 reposts
Using a chemoproteomic competition assay, we previously identified MBLAC2 as off-target of more than 20/50 profiled HDAC inhibitors (www.nature.com/articles/s41...)
Target deconvolution of HDAC pharmacopoeia reveals MBLAC2 as common off-target - Nature Chemical Biology
Lechner et al. used an affinity-based chemical proteomics approach to investigate the target landscape of HDAC inhibitors, and identified an extracellular vesicle regulator MBLAC2 as a universal off-t...
www.nature.com
May 9, 2025 at 12:00 PM
Everybody can reply
New paper!
Our chemoproteomic survey of phenylhydroxamic acids identifies the first drug-like inhibitors for nucleotide-binding protein HINT1 and nucleoside kinases NME1-4. On top, we found probes for the HDAC inhibitor off-target MBLAC2. Check out the open-access article! tinyurl.com/yh92vh6b
Our chemoproteomic survey of phenylhydroxamic acids identifies the first drug-like inhibitors for nucleotide-binding protein HINT1 and nucleoside kinases NME1-4. On top, we found probes for the HDAC inhibitor off-target MBLAC2. Check out the open-access article! tinyurl.com/yh92vh6b
May 9, 2025 at 12:41 PM
Everybody can reply
1 reposts
6 likes
Join us on the first day at #ASMS2025 🥳 Dr. Wei (Wilson) Li will be presenting an exciting oral on a novel chemoproteomic platform for reliable citrullination mapping in Alzheimer’s disease 🤓 Plus, don’t miss out 5 posters covering proteomics, Mass Spec Imaging, and ion mobility 🎉
June 2, 2025 at 10:10 AM
Everybody can reply
1 reposts
1 quotes
2 likes
Chemoproteomic discovery of a covalent allosteric inhibitor of WRN helicase https://covalentmodifiers.blogspot.com/2024/04/chemoproteomic-discovery-of-covalent.html
April 25, 2024 at 3:00 PM
Everybody can reply
PXD059664 🚨
Characterization and chemoproteomic profiling of protein O-linked -N-acetylglucosamine in SOD1-G93A mice
🚨 New dataset alert! 🚨
Characterization and chemoproteomic profiling of protein O-linked -N-acetylglucosamine in SOD1-G93A mice
🚨 New dataset alert! 🚨
June 6, 2025 at 7:00 PM
Everybody can reply