6/10
We next used cryoCLEM to quantify the vesicle movement as it approaches the plasma membrane...surprise, it is not a linear movement! Multiplying the original cryoCLEM data that we had in the preprint, we found out that vesicles pause at 27, 18 and 5 nm from the plasma membrane
We next used cryoCLEM to quantify the vesicle movement as it approaches the plasma membrane...surprise, it is not a linear movement! Multiplying the original cryoCLEM data that we had in the preprint, we found out that vesicles pause at 27, 18 and 5 nm from the plasma membrane
January 16, 2026 at 5:28 PM
6/10
We next used cryoCLEM to quantify the vesicle movement as it approaches the plasma membrane...surprise, it is not a linear movement! Multiplying the original cryoCLEM data that we had in the preprint, we found out that vesicles pause at 27, 18 and 5 nm from the plasma membrane
We next used cryoCLEM to quantify the vesicle movement as it approaches the plasma membrane...surprise, it is not a linear movement! Multiplying the original cryoCLEM data that we had in the preprint, we found out that vesicles pause at 27, 18 and 5 nm from the plasma membrane
Universitat Hamburg is hiring:
Research Associate for the Project “CryoCLEM sample transfer solutions to enable sustainable use of cryo-microscopes”
Research Associate for the Project “CryoCLEM sample transfer solutions to enable sustainable use of cryo-microscopes”
December 23, 2025 at 10:57 PM
Universitat Hamburg is hiring:
Research Associate for the Project “CryoCLEM sample transfer solutions to enable sustainable use of cryo-microscopes”
Research Associate for the Project “CryoCLEM sample transfer solutions to enable sustainable use of cryo-microscopes”
Dual-colour super-resolution cryoCLEM in mammalian cells using the fluorescent proteins rsTagRFP and rsEGFP2 pubmed.ncbi.nlm.nih.gov/41325867/ #cryoem
December 2, 2025 at 7:40 PM
Dual-colour super-resolution cryoCLEM in mammalian cells using the fluorescent proteins rsTagRFP and rsEGFP2 pubmed.ncbi.nlm.nih.gov/41325867/ #cryoem
Memory engram synapse 3D macromolecular architecture visualized by cryoCLEM-guided cryoET pubmed.ncbi.nlm.nih.gov/41240908/ #cryoem
November 16, 2025 at 1:55 PM
Memory engram synapse 3D macromolecular architecture visualized by cryoCLEM-guided cryoET pubmed.ncbi.nlm.nih.gov/41240908/ #cryoem
June 25, 2025 at 9:53 AM
Valuable advance in correlative #cryoCLEM - #CryoET imaging - especially applied to a challenging plant tissue!
Detailed workflow and beautiful ultrastructure details of plant cell and root barriers! ❄️🔬🌱
Must read for all cryoimaging enthusiasts!
#plantscience #microscopy #cryoimaging
Detailed workflow and beautiful ultrastructure details of plant cell and root barriers! ❄️🔬🌱
Must read for all cryoimaging enthusiasts!
#plantscience #microscopy #cryoimaging
Check out our latest work, featuring beautiful, first-of-their-kind cryo-tomograms of crucial plant cell wall modifications, Casparian strips, suberin lamellae, lignified xylem walls and more! From our Electron Microscopy Facility led by @chgenoud.bsky.social
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Imaging of specialized plant cell walls by improved cryo-CLEM and cryo-electron tomography
Cryo-focused ion beam scanning electron microscopy (cryo-FIBSEM) has become essential for preparing electron-transparent lamellae from cryo-plunged and high-pressure frozen specimens. However, targeti...
www.biorxiv.org
June 6, 2025 at 2:48 PM
Valuable advance in correlative #cryoCLEM - #CryoET imaging - especially applied to a challenging plant tissue!
Detailed workflow and beautiful ultrastructure details of plant cell and root barriers! ❄️🔬🌱
Must read for all cryoimaging enthusiasts!
#plantscience #microscopy #cryoimaging
Detailed workflow and beautiful ultrastructure details of plant cell and root barriers! ❄️🔬🌱
Must read for all cryoimaging enthusiasts!
#plantscience #microscopy #cryoimaging
We next used cryoCLEM with these fluorescent temporal-rulers to quantify the vesicle movement as it approaches the plasma membrane...surprise, it is not a linear movement!
February 12, 2025 at 11:42 AM
We next used cryoCLEM with these fluorescent temporal-rulers to quantify the vesicle movement as it approaches the plasma membrane...surprise, it is not a linear movement!
This study was key to establishing #cryoCLEM and #cryoET in Bordeaux.
It is an important milestone.
@davidp-bdx.bsky.social,
Étienne Herzog and @paulosh.bsky.social took the risk to be the first to use this approach with us.
🙏🙏🙏
It is an important milestone.
@davidp-bdx.bsky.social,
Étienne Herzog and @paulosh.bsky.social took the risk to be the first to use this approach with us.
🙏🙏🙏
New preprint from the MemTraS team ! www.biorxiv.org/content/10.1...
Great collaboration with @fronzeslab.bsky.social and Vladan Lucic to determine the first cryo electron tomography images of dopamine "synapses" extracted from adult mouse brain.
Great collaboration with @fronzeslab.bsky.social and Vladan Lucic to determine the first cryo electron tomography images of dopamine "synapses" extracted from adult mouse brain.
February 10, 2025 at 5:20 PM
This study was key to establishing #cryoCLEM and #cryoET in Bordeaux.
It is an important milestone.
@davidp-bdx.bsky.social,
Étienne Herzog and @paulosh.bsky.social took the risk to be the first to use this approach with us.
🙏🙏🙏
It is an important milestone.
@davidp-bdx.bsky.social,
Étienne Herzog and @paulosh.bsky.social took the risk to be the first to use this approach with us.
🙏🙏🙏
#Publication📗 Check out "Cryo-correlative light and electron tomography of dopaminergic axonal varicosities reveals non-synaptic modulation of cortico-striatal synapses"
👉Print focusing on #neuroscience, #CryoET and #CryoCLEM🧠🔬
www.biorxiv.org/content/10.1...
👉Print focusing on #neuroscience, #CryoET and #CryoCLEM🧠🔬
www.biorxiv.org/content/10.1...
February 10, 2025 at 10:50 AM
#Publication📗 Check out "Cryo-correlative light and electron tomography of dopaminergic axonal varicosities reveals non-synaptic modulation of cortico-striatal synapses"
👉Print focusing on #neuroscience, #CryoET and #CryoCLEM🧠🔬
www.biorxiv.org/content/10.1...
👉Print focusing on #neuroscience, #CryoET and #CryoCLEM🧠🔬
www.biorxiv.org/content/10.1...
RRID:Addgene_26969, was just reported to be used in "Memory engram synapse 3D molecular architecture visualized by cryoCLEM-guided cryoET". We appreciate the author's support of reproducibility. #ReproducibleResearch #methodsmatter
doi.org
February 8, 2025 at 8:01 AM
RRID:Addgene_26969, was just reported to be used in "Memory engram synapse 3D molecular architecture visualized by cryoCLEM-guided cryoET". We appreciate the author's support of reproducibility. #ReproducibleResearch #methodsmatter
Memory engram synapse 3D molecular architecture visualized by cryoCLEM-guided cryoET pubmed.ncbi.nlm.nih.gov/39829918/
January 22, 2025 at 3:53 AM
Memory engram synapse 3D molecular architecture visualized by cryoCLEM-guided cryoET pubmed.ncbi.nlm.nih.gov/39829918/
Now research engineer and CryoCLEM/ET master @iecb.bsky.social @robinanger.bsky.social joined BlueSky.
Welcome !
Welcome !
January 16, 2025 at 4:58 PM
Now research engineer and CryoCLEM/ET master @iecb.bsky.social @robinanger.bsky.social joined BlueSky.
Welcome !
Welcome !
Memory engram synapse 3D molecular architecture visualized by cryoCLEM-guided cryoET www.biorxiv.org/content/10.1... - super cool work from my colleague @tjryan.bsky.social and collaborators
January 15, 2025 at 4:02 PM
Memory engram synapse 3D molecular architecture visualized by cryoCLEM-guided cryoET www.biorxiv.org/content/10.1... - super cool work from my colleague @tjryan.bsky.social and collaborators
Memory engram synapse 3D molecular architecture visualized by cryoCLEM-guided cryoET https://www.biorxiv.org/content/10.1101/2025.01.09.632151v1
January 12, 2025 at 11:15 PM
Memory engram synapse 3D molecular architecture visualized by cryoCLEM-guided cryoET https://www.biorxiv.org/content/10.1101/2025.01.09.632151v1
Memory engram synapse 3D molecular architecture visualized by cryoCLEM-guided cryoET https://www.biorxiv.org/content/10.1101/2025.01.09.632151v1
January 12, 2025 at 11:15 PM
Memory engram synapse 3D molecular architecture visualized by cryoCLEM-guided cryoET https://www.biorxiv.org/content/10.1101/2025.01.09.632151v1
December 21, 2024 at 8:26 AM
Sincere congratulations to our colleague Paul Lapios @paulosh.bsky.social on his thesis defence this morning.
I'm very pleased to see that all the efforts made to get the #CryoCLEM and #CryoET workflows up and running in Bordeaux have paid off and are producing meaningful results.
I'm very pleased to see that all the efforts made to get the #CryoCLEM and #CryoET workflows up and running in Bordeaux have paid off and are producing meaningful results.
December 6, 2024 at 1:09 PM
Sincere congratulations to our colleague Paul Lapios @paulosh.bsky.social on his thesis defence this morning.
I'm very pleased to see that all the efforts made to get the #CryoCLEM and #CryoET workflows up and running in Bordeaux have paid off and are producing meaningful results.
I'm very pleased to see that all the efforts made to get the #CryoCLEM and #CryoET workflows up and running in Bordeaux have paid off and are producing meaningful results.
#IINSPhD🧑🎓 @paulosh.bsky.social, PhD student in David Perrais' team, #MemTraS, defends his thesis: “Ultrastructural and molecular analysis of cortico-striatal dopamine hub synapses”
He worked on the #synapse, #dopamine, #striatum and #cryoCLEM
www.bordeaux-neurocampus.fr/event/souten...
He worked on the #synapse, #dopamine, #striatum and #cryoCLEM
www.bordeaux-neurocampus.fr/event/souten...
Soutenance de thèse - Paul Lapios - Bordeaux Neurocampus
Ultrastructural and molecular analysis of cortico-striatal dopamine hub synapses // Lieu : CARF
www.bordeaux-neurocampus.fr
December 6, 2024 at 8:28 AM
#IINSPhD🧑🎓 @paulosh.bsky.social, PhD student in David Perrais' team, #MemTraS, defends his thesis: “Ultrastructural and molecular analysis of cortico-striatal dopamine hub synapses”
He worked on the #synapse, #dopamine, #striatum and #cryoCLEM
www.bordeaux-neurocampus.fr/event/souten...
He worked on the #synapse, #dopamine, #striatum and #cryoCLEM
www.bordeaux-neurocampus.fr/event/souten...
Dusting off a classic for #FluorescenceFriday: A stunning Blender render by Daniel Stabley of a neuron nucleus I imaged with our colleagues at Janelia. CryoCLEM reveals H3.3 euchromatin (blue) + HP1 heterochromatin (purple), by merging super-res #microscopy with FIB-SEM! #neuroscience #imaging
November 15, 2024 at 12:48 PM
Dusting off a classic for #FluorescenceFriday: A stunning Blender render by Daniel Stabley of a neuron nucleus I imaged with our colleagues at Janelia. CryoCLEM reveals H3.3 euchromatin (blue) + HP1 heterochromatin (purple), by merging super-res #microscopy with FIB-SEM! #neuroscience #imaging
Some news about the CryoCLEM workshop that took place at the Bordeaux Imaging Center and @iecb.bsky.social some weeks ago.
Organized by Noemie Pied, @monica-bic.bsky.social and Robin Anger.
(sorry only in French)
Organized by Noemie Pied, @monica-bic.bsky.social and Robin Anger.
(sorry only in French)
Organisation d’un workshop sur la cryo-microscopie corrélative photonique-électronique (CryoCLEM) à l’Institut Européen de Chimie et Biologie (IECB) et au Bordeaux Imaging Center (BIC) - IECB | FR
Publié le 12/11/2024
www.iecb.u-bordeaux.fr
November 14, 2024 at 8:40 AM
Some news about the CryoCLEM workshop that took place at the Bordeaux Imaging Center and @iecb.bsky.social some weeks ago.
Organized by Noemie Pied, @monica-bic.bsky.social and Robin Anger.
(sorry only in French)
Organized by Noemie Pied, @monica-bic.bsky.social and Robin Anger.
(sorry only in French)
More like cryoCLEM apprenticing 😅
November 12, 2024 at 12:20 PM
More like cryoCLEM apprenticing 😅
Some cryoCLEM magic going on here !!
November 12, 2024 at 12:12 PM
Some cryoCLEM magic going on here !!
Last talk of the #CryoFIBSEMMethodsDevelopment session is "cryoCLEM FIB Volume Imaging: No-Contact Sample Transfer" with Abigail Lytton-Jean. A really elegant solution to sample movement around the #CLEM pipeline.
October 22, 2024 at 1:27 PM
Last talk of the #CryoFIBSEMMethodsDevelopment session is "cryoCLEM FIB Volume Imaging: No-Contact Sample Transfer" with Abigail Lytton-Jean. A really elegant solution to sample movement around the #CLEM pipeline.
#lorneproteins V early Sesh 3 @GarciaSaez_Lab BAX/BAK mit permeabilisation BAX | ( 💍 super res fluo 🔬 Mechanism? BAK fast BAX slow. Coassembly mitDNA release faster together 👭 cryoCLEM/Tomo-pores in outer membrane only. Osmotic pressure ⏫️ inner membrane extrusion. 🤩👏🫶
November 20, 2024 at 4:39 PM
#lorneproteins V early Sesh 3 @GarciaSaez_Lab BAX/BAK mit permeabilisation BAX | ( 💍 super res fluo 🔬 Mechanism? BAK fast BAX slow. Coassembly mitDNA release faster together 👭 cryoCLEM/Tomo-pores in outer membrane only. Osmotic pressure ⏫️ inner membrane extrusion. 🤩👏🫶