Cell-cycle inhibition preserves robust development but rebalances lineages in mouse gastruloids #SingleCell 🧪🧬🖥️
https://www.biorxiv.org/content/10.64898/2026.01.08.698406v1
https://www.biorxiv.org/content/10.64898/2026.01.08.698406v1
January 9, 2026 at 8:00 AM
Cell-cycle inhibition preserves robust development but rebalances lineages in mouse gastruloids #SingleCell 🧪🧬🖥️
https://www.biorxiv.org/content/10.64898/2026.01.08.698406v1
https://www.biorxiv.org/content/10.64898/2026.01.08.698406v1
Cell-cycle inhibition preserves robust development but rebalances lineages in mouse gastruloids https://www.biorxiv.org/content/10.64898/2026.01.08.698406v1
January 9, 2026 at 3:31 AM
Cell-cycle inhibition preserves robust development but rebalances lineages in mouse gastruloids https://www.biorxiv.org/content/10.64898/2026.01.08.698406v1
Cell-cycle inhibition preserves robust development but rebalances lineages in mouse gastruloids https://www.biorxiv.org/content/10.64898/2026.01.08.698406v1
January 9, 2026 at 3:31 AM
Cell-cycle inhibition preserves robust development but rebalances lineages in mouse gastruloids https://www.biorxiv.org/content/10.64898/2026.01.08.698406v1
Zong-Yuan Liu of @umich.edu will round out the #SDBPostdocSeminar with his talk "Endogenous FGFs drive ERK-dependent cell fate patterning in 2D human gastruloids" Image: FGF4 (blue), FGF8 (green) and primitive streak-like cells (red) in 2D human gastruloids
January 7, 2026 at 2:51 PM
Zong-Yuan Liu of @umich.edu will round out the #SDBPostdocSeminar with his talk "Endogenous FGFs drive ERK-dependent cell fate patterning in 2D human gastruloids" Image: FGF4 (blue), FGF8 (green) and primitive streak-like cells (red) in 2D human gastruloids
The next the Ethel Browne Harvey Postdoc Seminar will be this Friday, January 9 at 3 pm Eastern Time with Priyom Adhyapok @priyomadhyapok.bsky.social @duke-university.bsky.social and Zong-Yuan Liu @UMich. Register today and join us. bit.ly/4ncxr0M #SDBPostdocSeminar
January 7, 2026 at 2:51 PM
The next the Ethel Browne Harvey Postdoc Seminar will be this Friday, January 9 at 3 pm Eastern Time with Priyom Adhyapok @priyomadhyapok.bsky.social @duke-university.bsky.social and Zong-Yuan Liu @UMich. Register today and join us. bit.ly/4ncxr0M #SDBPostdocSeminar
Catherine Robin has received an LSBR grant to advance a promising approach to blood stem cell generation. This project aims to establish gastruloids as an animal-free model to study blood stem cell development and enable scalable production of these cells. See www.hubrecht.eu/lsbr-grant-r...
January 7, 2026 at 1:06 PM
Catherine Robin has received an LSBR grant to advance a promising approach to blood stem cell generation. This project aims to establish gastruloids as an animal-free model to study blood stem cell development and enable scalable production of these cells. See www.hubrecht.eu/lsbr-grant-r...
@kstapornwongkul.bsky.social , #newPI at @viennabiocenter.bsky.social - discussing the metabolism of embryonic development using #gastruloids. #embryogenesis
#DevBio #stemcell #glucosemetabolism
#DevBio #stemcell #glucosemetabolism
Metabolic Origins: Steering of early developmental fate #MetabolismMondays - the Node
All the world's a metabolic dance, early career scientists are leading the way! Emerging perspectives in metabolism Dr Kristina StapornwongkulX:
thenode.biologists.com
December 23, 2025 at 2:36 AM
@kstapornwongkul.bsky.social , #newPI at @viennabiocenter.bsky.social - discussing the metabolism of embryonic development using #gastruloids. #embryogenesis
#DevBio #stemcell #glucosemetabolism
#DevBio #stemcell #glucosemetabolism
1/2 Using #gastruloids @AnitaBanerjee19 Ram Sambasivan group find that Retinoic Acid gates the response of the epiblast to the Nodal:Eomes, Wnt:TbxT modules during mouse #gastrulation promoting the regionalization of the #PrimitiveStreak www.biorxiv.org/content/10.6...
December 16, 2025 at 5:02 AM
1/2 Using #gastruloids @AnitaBanerjee19 Ram Sambasivan group find that Retinoic Acid gates the response of the epiblast to the Nodal:Eomes, Wnt:TbxT modules during mouse #gastrulation promoting the regionalization of the #PrimitiveStreak www.biorxiv.org/content/10.6...
At InSDB-SD2026 in IISER Berhampur
Great Day 2 morning session with a highlight from @InamdarManeesha. on the use of #SCbEM in the study of #HumanReproduction within the CReATE initiative @DBT_inStem
Using #Gastruloids for human health
Great Day 2 morning session with a highlight from @InamdarManeesha. on the use of #SCbEM in the study of #HumanReproduction within the CReATE initiative @DBT_inStem
Using #Gastruloids for human health
December 15, 2025 at 5:48 PM
At InSDB-SD2026 in IISER Berhampur
Great Day 2 morning session with a highlight from @InamdarManeesha. on the use of #SCbEM in the study of #HumanReproduction within the CReATE initiative @DBT_inStem
Using #Gastruloids for human health
Great Day 2 morning session with a highlight from @InamdarManeesha. on the use of #SCbEM in the study of #HumanReproduction within the CReATE initiative @DBT_inStem
Using #Gastruloids for human health
Thank you @InamdarManeesha for hosting my visit to CREATE, an initiative within @DBT_inStem devoted to advancing knowledge and empowering innovation on #Human#Development
instem.res.in/research/maj...
an amazing project
#Fertility #Blastoids #Gastruloids
instem.res.in/research/maj...
an amazing project
#Fertility #Blastoids #Gastruloids
December 13, 2025 at 2:54 AM
Thank you @InamdarManeesha for hosting my visit to CREATE, an initiative within @DBT_inStem devoted to advancing knowledge and empowering innovation on #Human#Development
instem.res.in/research/maj...
an amazing project
#Fertility #Blastoids #Gastruloids
instem.res.in/research/maj...
an amazing project
#Fertility #Blastoids #Gastruloids
4/
Using scRNA-seq, 3D-hE-gastruloids map directly onto in vivo post-implantation epiblast and early primitive streak.
They express:
🔹 Pluripotency: OCT4, SOX2, PRDM14
🔹 Epithelial/polarity: CDH1, CLDN6/7, PODXL
🔹 PS markers: TBXT, MIXL1, EOMES (but only in a subset)
Using scRNA-seq, 3D-hE-gastruloids map directly onto in vivo post-implantation epiblast and early primitive streak.
They express:
🔹 Pluripotency: OCT4, SOX2, PRDM14
🔹 Epithelial/polarity: CDH1, CLDN6/7, PODXL
🔹 PS markers: TBXT, MIXL1, EOMES (but only in a subset)
December 11, 2025 at 1:32 PM
4/
Using scRNA-seq, 3D-hE-gastruloids map directly onto in vivo post-implantation epiblast and early primitive streak.
They express:
🔹 Pluripotency: OCT4, SOX2, PRDM14
🔹 Epithelial/polarity: CDH1, CLDN6/7, PODXL
🔹 PS markers: TBXT, MIXL1, EOMES (but only in a subset)
Using scRNA-seq, 3D-hE-gastruloids map directly onto in vivo post-implantation epiblast and early primitive streak.
They express:
🔹 Pluripotency: OCT4, SOX2, PRDM14
🔹 Epithelial/polarity: CDH1, CLDN6/7, PODXL
🔹 PS markers: TBXT, MIXL1, EOMES (but only in a subset)
3/
This model, called “3D‑hE‑gastruloids” is powerfull because it eliminates undefined factors and extraembryonic cells, allowing us to precisely test the signals that govern epithelial identity and PS induction.
Reproducibility is high: >95% of structures form a correct lumen by day 4.
This model, called “3D‑hE‑gastruloids” is powerfull because it eliminates undefined factors and extraembryonic cells, allowing us to precisely test the signals that govern epithelial identity and PS induction.
Reproducibility is high: >95% of structures form a correct lumen by day 4.
December 11, 2025 at 1:32 PM
3/
This model, called “3D‑hE‑gastruloids” is powerfull because it eliminates undefined factors and extraembryonic cells, allowing us to precisely test the signals that govern epithelial identity and PS induction.
Reproducibility is high: >95% of structures form a correct lumen by day 4.
This model, called “3D‑hE‑gastruloids” is powerfull because it eliminates undefined factors and extraembryonic cells, allowing us to precisely test the signals that govern epithelial identity and PS induction.
Reproducibility is high: >95% of structures form a correct lumen by day 4.
YAP levels regulate anteroposterior elongation of hESC-derived gastruloids #SingleCell 🧪🧬🖥️
https://www.biorxiv.org/content/10.1101/10.64898/2025.12.01.691265v1
https://www.biorxiv.org/content/10.1101/10.64898/2025.12.01.691265v1
December 3, 2025 at 8:00 AM
YAP levels regulate anteroposterior elongation of hESC-derived gastruloids #SingleCell 🧪🧬🖥️
https://www.biorxiv.org/content/10.1101/10.64898/2025.12.01.691265v1
https://www.biorxiv.org/content/10.1101/10.64898/2025.12.01.691265v1
YAP levels regulate anteroposterior elongation of hESC-derived gastruloids https://www.biorxiv.org/content/10.64898/2025.12.01.691265v1
December 3, 2025 at 1:30 AM
YAP levels regulate anteroposterior elongation of hESC-derived gastruloids https://www.biorxiv.org/content/10.64898/2025.12.01.691265v1
YAP levels regulate anteroposterior elongation of hESC-derived gastruloids https://www.biorxiv.org/content/10.64898/2025.12.01.691265v1
December 3, 2025 at 1:30 AM
YAP levels regulate anteroposterior elongation of hESC-derived gastruloids https://www.biorxiv.org/content/10.64898/2025.12.01.691265v1
#N2B27 is an acronym that creates a lot of trouble in labs growing #gastruloids because of variability. Building on earlier work from A. Smith’s lab, my colleagues decided to address the root of the issue shorturl.at/QM4C6
and see their views on the work shorturl.at/dYB9X
and see their views on the work shorturl.at/dYB9X
November 28, 2025 at 9:02 AM
#N2B27 is an acronym that creates a lot of trouble in labs growing #gastruloids because of variability. Building on earlier work from A. Smith’s lab, my colleagues decided to address the root of the issue shorturl.at/QM4C6
and see their views on the work shorturl.at/dYB9X
and see their views on the work shorturl.at/dYB9X
🧩 What breaks when you remove DNA loop extrusion?
Gastruloids show it: not lineage decisions… but morphogenesis.
That’s our contribution to www.science.org/doi/10.1126/...
Huge thanks to the amazing @karissalhansen.bsky.social + @elphegenoralab.bsky.social for leading this fantastic work 🙌
Gastruloids show it: not lineage decisions… but morphogenesis.
That’s our contribution to www.science.org/doi/10.1126/...
Huge thanks to the amazing @karissalhansen.bsky.social + @elphegenoralab.bsky.social for leading this fantastic work 🙌
(5) But can embryonic cell types properly emerge even when loop extrusion is prevented?
Unambiguous gastruloid experiments by
@bracciolilab.bsky.social
in
@dewitlab.bsky.social
: YES, they clearly can.
Even though morphogenesis, which is normally very stereotypical, is pretty screwed up.
Unambiguous gastruloid experiments by
@bracciolilab.bsky.social
in
@dewitlab.bsky.social
: YES, they clearly can.
Even though morphogenesis, which is normally very stereotypical, is pretty screwed up.
November 28, 2025 at 8:57 AM
🧩 What breaks when you remove DNA loop extrusion?
Gastruloids show it: not lineage decisions… but morphogenesis.
That’s our contribution to www.science.org/doi/10.1126/...
Huge thanks to the amazing @karissalhansen.bsky.social + @elphegenoralab.bsky.social for leading this fantastic work 🙌
Gastruloids show it: not lineage decisions… but morphogenesis.
That’s our contribution to www.science.org/doi/10.1126/...
Huge thanks to the amazing @karissalhansen.bsky.social + @elphegenoralab.bsky.social for leading this fantastic work 🙌
The power of the media: core culture conditions direct gastruloid development
This Research Highlight showcases the work from Tina Balayo, André Dias @dias-andre.bsky.social, David Turner @gastruloids.bsky.social and colleagues:
journals.biologists.com/dev/article/...
This Research Highlight showcases the work from Tina Balayo, André Dias @dias-andre.bsky.social, David Turner @gastruloids.bsky.social and colleagues:
journals.biologists.com/dev/article/...
![NDiff227 gastruloids display a developmental delay in comparison to HM-N2B27 gastruloids. (A) Quantitative analysis of the roundness (perimeter2/4π×area) of gastruloids cultured in either HM-N2B27 or NDiff227 showed that HM-N2B27 gastruloids are significantly less ‘Round’ than their NDiff227 counterparts at 96 h [unpaired two-tailed t-test; t(4)=−4.9541, P=0.008]. Each datapoint is the average roundness of an independent replicate experiment containing between ∼40 and 90 individual gastruloids (see Fig. S4B). (B) The frequency of morphologies from the data in A, with examples of gastruloids that would fall in each category (bottom; the left 'Protrusions' image is also shown in Fig. S4). (C) Light-sheet representative images (maximum projection images) highlighting the expression patterns of Cdh1 (magenta) and Cdh2 (cyan) between HM-N2B27 gastruloids at 96 h and NDiff227 gastruloids at 90 h and 96 h. Gastruloids developed in HM-N2B27 have upregulated Cdh2 by 90 h, unlike NDiff227 gastruloids, which show enhanced Cdh2 only at 96 h. Scale bars: 100 μm. (Ci) Qualitative analysis of the average expression pattern of Cdh2 (N-cadherin). The expression patterns were grouped into two categories (phenotypes I and II) and represent the average of three independent replicate experiments, each containing at least seven individual gastruloids per condition. Examples of the two categories are shown below. The leftmost example for ‘Phenotype I’ is shown in the middle row of 90 h HM-N2B27 (Cdh2) in C. For the box and whisker plot, the central line in the box is the median, the lower edge of the box is the first quartile, the upper edge of the box is the third quartile, and the box height is the IQR. The lower and upper whiskers indicate the smallest datapoint within 1.5xIQR below Q1 or above Q3, respectively.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:btitwyhiegkb6f62oguxt6m6/bafkreiakq7wvgnsnajj2g3bpn5k5p4yu7vznwibobhgzu2o5ttsmjq5ylq@jpeg)
November 26, 2025 at 4:13 PM
The power of the media: core culture conditions direct gastruloid development
This Research Highlight showcases the work from Tina Balayo, André Dias @dias-andre.bsky.social, David Turner @gastruloids.bsky.social and colleagues:
journals.biologists.com/dev/article/...
This Research Highlight showcases the work from Tina Balayo, André Dias @dias-andre.bsky.social, David Turner @gastruloids.bsky.social and colleagues:
journals.biologists.com/dev/article/...
Huge thanks to @denisduboule.bsky.social for an inspiring talk on Hox genes role in mammalian patterning in gastruloids. Brilliant presentation!
🙏🏼 A wonderful meeting indeed. Full of great science and energy 👏 @fsscr.bsky.social
Such a treat to be sitting in the room where @denisduboule.bsky.social is giving a talk @fsscr.bsky.social meeting in Lyon! Exciting and insightful science ahead 🤯🤘
November 19, 2025 at 4:36 PM
Huge thanks to @denisduboule.bsky.social for an inspiring talk on Hox genes role in mammalian patterning in gastruloids. Brilliant presentation!
We will build and apply new opto-rheological and rheomicroscopy tools to study:
• Biomimetic emulsions and vesicle systems
• Zebrafish gastruloids
In collaboration with:
— @dianapinheiro.bsky.social (IMP Vienna)
— @ehannezo.bsky.social (ISTA)
• Biomimetic emulsions and vesicle systems
• Zebrafish gastruloids
In collaboration with:
— @dianapinheiro.bsky.social (IMP Vienna)
— @ehannezo.bsky.social (ISTA)
November 18, 2025 at 9:00 AM
We will build and apply new opto-rheological and rheomicroscopy tools to study:
• Biomimetic emulsions and vesicle systems
• Zebrafish gastruloids
In collaboration with:
— @dianapinheiro.bsky.social (IMP Vienna)
— @ehannezo.bsky.social (ISTA)
• Biomimetic emulsions and vesicle systems
• Zebrafish gastruloids
In collaboration with:
— @dianapinheiro.bsky.social (IMP Vienna)
— @ehannezo.bsky.social (ISTA)
We generated mosaic gastruloids with varying proportion of mutant cells to force differential N-Cadherin expression. Interestingly, the elongation was directly correlated to the proportion of mutant cells, linking gastruloid elongation to cell adhesion. 4/12
November 17, 2025 at 10:37 AM
We generated mosaic gastruloids with varying proportion of mutant cells to force differential N-Cadherin expression. Interestingly, the elongation was directly correlated to the proportion of mutant cells, linking gastruloid elongation to cell adhesion. 4/12
Here, we could confirm that gastruloids lacking N-Cadherin self-organize correctly, in fact even better than their wildtype counter part as they form polarized somitic tissue without requiring exogeneous extra cellular matrix such as matrigel. 2/12
November 17, 2025 at 10:37 AM
Here, we could confirm that gastruloids lacking N-Cadherin self-organize correctly, in fact even better than their wildtype counter part as they form polarized somitic tissue without requiring exogeneous extra cellular matrix such as matrigel. 2/12
This morning we hosted David Brückner (Biozentrum, Univ. Basel) — chaired by James Sharpe (EMBL Barcelona) — for a tour of self-organization in development via dynamical systems + information theory, with concrete case studies on organoids and gastruloids.
November 6, 2025 at 1:22 PM
This morning we hosted David Brückner (Biozentrum, Univ. Basel) — chaired by James Sharpe (EMBL Barcelona) — for a tour of self-organization in development via dynamical systems + information theory, with concrete case studies on organoids and gastruloids.
@campaslab.bsky.social @jesseveenvliet.bsky.social labs use #gastruloids to explore axial extension in mammalian embryos
www.biorxiv.org/content/10.1...
They find novel mechanisms involving and interplay of proliferation and mechanical constraining.
#NotInTheGenes #CellsRUs
www.biorxiv.org/content/10.1...
They find novel mechanisms involving and interplay of proliferation and mechanical constraining.
#NotInTheGenes #CellsRUs
November 6, 2025 at 6:40 AM
@campaslab.bsky.social @jesseveenvliet.bsky.social labs use #gastruloids to explore axial extension in mammalian embryos
www.biorxiv.org/content/10.1...
They find novel mechanisms involving and interplay of proliferation and mechanical constraining.
#NotInTheGenes #CellsRUs
www.biorxiv.org/content/10.1...
They find novel mechanisms involving and interplay of proliferation and mechanical constraining.
#NotInTheGenes #CellsRUs