This study introduces single-cell transcription factor (TF) sequencing, a single-cell barcoded and doxycycline-inducible TF overexpression approach that reveals dose-sensitive functional classes of TFs and cellular heterogeneity by mapping TF dose-dependent transcriptomic changes during the reprogramming of mouse embryonic multipotent stromal cells.

Le développement embryonnaire ne requiert pas uniquement l’activation des gènes, mais également une régulation fine de leur répression.

Bioinformatician / Data scientist position in the Aztekin Lab focusing on Structural Regeneration and support the working group together with the other members

A small-molecule-only strategy enables reprogramming ESCs into embryo founder cells that give rise to a high-fidelity embryo model directly and efficiently. This embryo model replicates embryogenesis ...

Hanna and colleagues show that signaling pathway modulation amplifies the inherent flexibility of genetically unmodified mouse naive PSCs to adopt extra-embryonic cell fate potential. This endows them...

Hanna and colleagues show that signaling pathway modulation amplifies the inherent flexibility of genetically unmodified mouse naive PSCs to adopt extra-embryonic cell fate potential. This endows them with the competence to generate up to E8.5–E8.75 transgene-free stem cell-derived embryo models (TF-SEMs) that complete gastrulation and initiate organogenesis within complex extra-embryonic compartments ex utero.

Activation of an LTR retrotransposon inserted upstream of the Fgf8 gene produces viral-like particles in the mouse developing limb, triggering apoptosis and causing limb malformation. This phenotype c...

Here the authors show that reducing enhancer-promoter distance at the Pitx1 locus increases proportion of Pitx1 forelimb expressing cells, worsening skeletal defects in Liebenberg syndrome. They also ...

The REX element is associated with long-range enhancer–promoter interactions.