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Ellenberg Lab @ellenberglab.bsky.social · Sep 10

Our work on NPC biogenesis is now out! Wanlu Zhang investigated the role of FG-Nups in NPC reassembly, pore dilation, and the nuclear envelope remodelling using acute depletions, correlative EM, and MINFLUX imaging 💫.
Thanks, Wanlu and all the collaborators!

Wanlu Zhang @wanluzhang.bsky.social · Sep 9

Excited to share the preprint from my postdoc work @ellenberglab.bsky.social! 🌟
During mitotic exit, cells rebuild thousands of nuclear pores within minutes. How do tiny holes expand to selective channels, and how is this coordinated with the nuclear envelope remodeling? 🔗 doi.org/10.1101/2025...

Hydrophobic interactions of FG-nucleoporins are required for dilating nuclear membrane pores into selective transport channels after mitosis

Nuclear envelope (NE) reformation after mitosis is essential for daughter cell viability and requires tightly coordinated nuclear pore complex (NPC) assembly and nuclear membrane reformation. To revea...

doi.org

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Reposted by Ellenberg Lab

Florian Schueder @florianschueder.bsky.social · Jul 23

🔬🧬 New in @natcomms.nature.com‬: Nanoscale 3D DNA tracing in non‑denatured cells maps the #3Dgenome at nm‑resolution and reveals Cohesin‑dependent dynamic loops! 🚀 #OpenAccess 

www.nature.com/articles/s41...

@ellenberglab.bsky.social @jungmannlab.bsky.social
#Genomics #SuperResolution

Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ - Nature Communications

A nanoscale 3D DNA tracing workflow visualizes Cohesin-dependent loops in single, structurally well-preserved cells. Computer simulations based on the tracing data give further insight into how Cohesi...

www.nature.com

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Ellenberg Lab @ellenberglab.bsky.social · Jul 21

Thanks Aleksander, also for your paper link!

Ellenberg Lab @ellenberglab.bsky.social · Jul 21

Our new non-denaturing nanoscale 3D DNA tracing technology provides single-cell (and sister-chromatid specific!) readout of chromatin structure with 10kb genomic resolution - Check it out!

www.nature.com/articles/s41...

Congratulations to Kai and Oyvind!!

Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ - Nature Communications

A nanoscale 3D DNA tracing workflow visualizes Cohesin-dependent loops in single, structurally well-preserved cells. Computer simulations based on the tracing data give further insight into how Cohesi...

www.nature.com

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Reposted by Ellenberg Lab

Anders Sejr Hansen @andersshansen.bsky.social · Jul 20

This seems like a really important paper comparing chromosome tracing methods, demonstrating that classical denaturing DNA-FISH and chromosome tracing leads to substantial distortion of 3D genome structure, likely due to the high-temperatures involved (70-90C):
www.nature.com/articles/s41...

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Ellenberg Lab @ellenberglab.bsky.social · Mar 25

Thank you very much, Takashi!

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Ellenberg Lab @ellenberglab.bsky.social · Mar 24

Check out Andi's #Bluetorial for a summary of our findings 🙌

Andi Brunner @andibrunner.bsky.social · Mar 24

Mitotic chromosomes form through long, overlapping and self-repelling DNA loops, generated by the Condensin complexes!

Check out our newest paper here, and see below for a short Bluetorial!
www.cell.com/cell/fulltex...

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Reposted by Ellenberg Lab

EMBL @embl.org · Mar 24

Did you know that DNA must form overlapping loops to give rise to the well-known X-shape of chromosomes? 🧬🧪

New research from EMBL's Ellenberg Group provides new insights into the process of chromosome formation.

@ellenberglab.bsky.social
@andibrunner.bsky.social

www.embl.org/news/science...

How chromosomes shape up for cell division | EMBL

EMBL scientists have shown how overlapping loops of DNA stack upon each other in dividing cells to give rise to rod-shaped chromosomes

www.embl.org

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Reposted by Ellenberg Lab

Ellenberg Lab @ellenberglab.bsky.social · Mar 24

Our new findings on how chromosomes get ready for cell division are now published in @cellpress.bsky.social!

Congratulations, Kai, @andibrunner.bsky.social and everyone else involved! 🤩

www.sciencedirect.com/science/arti...

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Ellenberg Lab @ellenberglab.bsky.social · Mar 24

Our new findings on how chromosomes get ready for cell division are now published in @cellpress.bsky.social!

Congratulations, Kai, @andibrunner.bsky.social and everyone else involved! 🤩

www.sciencedirect.com/science/arti...

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Ellenberg Lab @ellenberglab.bsky.social · Mar 19

We are #hiring!

We have two open postdoc/research scientist positions to join our #ERC funded project MITOFOLD to resolve the organization of mitotic chromosomes!

1) experimental biologist shorturl.at/LjGEM
2) data scientist shorturl.at/nDdhQ
Please share! 🙌

We look forward to your application! 🤩

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Ellenberg Lab @ellenberglab.bsky.social · Jan 9

Congratulations to @andibrunner.bsky.social
and the team, and many thanks to the lab of Jan-Michael Peters for support!

We found that the interphase and mitotic chromatin loop organization have more in common than previously thought:

In both stages big loops are built first, small ones second

Journal of Cell Biology @jcb.org · Jan 9

@andibrunner.bsky.social et al. @ellenberglab.bsky.social show that interphase organization into Cohesin-driven loops occurs in a sequential and hierarchical manner - just like the mitotic organization by Condensins. https://buff.ly/426gIEu
@wanluzhang.bsky.social

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Reposted by Ellenberg Lab

Andi Brunner @andibrunner.bsky.social · Jan 9

My first own project is published:

Despite major differences in chromosome shape during #interphase and #mitosis, the underlying #loop-extruder based organization is highly similar

A new data resource and new insights into human chromatin biology! 🧬🥳

Journal of Cell Biology @jcb.org · Jan 9

@andibrunner.bsky.social et al. @ellenberglab.bsky.social show that interphase organization into Cohesin-driven loops occurs in a sequential and hierarchical manner - just like the mitotic organization by Condensins. https://buff.ly/426gIEu
@wanluzhang.bsky.social

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Ellenberg Lab @ellenberglab.bsky.social · Dec 16

Our excellent postdoc Wanlu Zhang is at #CellBio24 and will talk about her unpublished NPC biogensis work tomorrow!

Check it out and say hello 🙌🏼

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Ellenberg Lab @ellenberglab.bsky.social · Nov 25

We are now on Bluesky!

Follow us for exciting #Science around #CellDivision and nuclear organization, illuminated using advanced #imaging #technology - like our last preprint on mitotic chromosome structure and organization: tinyurl.com/yaznnkku

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