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Reposted by Andi Brunner

Ellenberg Lab @ellenberglab.bsky.social · Sep 10

Our work on NPC biogenesis is now out! Wanlu Zhang investigated the role of FG-Nups in NPC reassembly, pore dilation, and the nuclear envelope remodelling using acute depletions, correlative EM, and MINFLUX imaging 💫.
Thanks, Wanlu and all the collaborators!

Wanlu Zhang @wanluzhang.bsky.social · Sep 9

Excited to share the preprint from my postdoc work @ellenberglab.bsky.social! 🌟
During mitotic exit, cells rebuild thousands of nuclear pores within minutes. How do tiny holes expand to selective channels, and how is this coordinated with the nuclear envelope remodeling? 🔗 doi.org/10.1101/2025...

Hydrophobic interactions of FG-nucleoporins are required for dilating nuclear membrane pores into selective transport channels after mitosis

Nuclear envelope (NE) reformation after mitosis is essential for daughter cell viability and requires tightly coordinated nuclear pore complex (NPC) assembly and nuclear membrane reformation. To revea...

doi.org

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Andi Brunner @andibrunner.bsky.social · Sep 10

Congratulations Wanlu!! 🚀🚀

Wanlu Zhang @wanluzhang.bsky.social · Sep 9

Excited to share the preprint from my postdoc work @ellenberglab.bsky.social! 🌟
During mitotic exit, cells rebuild thousands of nuclear pores within minutes. How do tiny holes expand to selective channels, and how is this coordinated with the nuclear envelope remodeling? 🔗 doi.org/10.1101/2025...

Hydrophobic interactions of FG-nucleoporins are required for dilating nuclear membrane pores into selective transport channels after mitosis

Nuclear envelope (NE) reformation after mitosis is essential for daughter cell viability and requires tightly coordinated nuclear pore complex (NPC) assembly and nuclear membrane reformation. To revea...

doi.org

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Jeremy Cliffe @jeremycliffe.bsky.social · Aug 24

Just got round to watching Mario Draghi's speech in Rimini in the original Italian. It is easily the most important speech on Europe so far this year and I hope someone (@grandcontinent.bsky.social?‬) publishes it in English and other EU languages soon.

www.youtube.com/watch?v=YMME...

Mario Draghi’s Full Speech: Europe’s Harsh Reality — Powerless on the Global Stage! -ITALIAN-

YouTube video by EU Debates | eudebates.tv

www.youtube.com

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Andi Brunner @andibrunner.bsky.social · Jul 21

Indeed as Ilya says, BrdU should only be added for a single S-phase. The longer you incubate with BrdU (the more replication cycles incorporate BrdU) the less available DNA you will habe after the RASER-FISH procedure :)

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Ellenberg Lab @ellenberglab.bsky.social · Jul 21

Our new non-denaturing nanoscale 3D DNA tracing technology provides single-cell (and sister-chromatid specific!) readout of chromatin structure with 10kb genomic resolution - Check it out!

www.nature.com/articles/s41...

Congratulations to Kai and Oyvind!!

Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ - Nature Communications

A nanoscale 3D DNA tracing workflow visualizes Cohesin-dependent loops in single, structurally well-preserved cells. Computer simulations based on the tracing data give further insight into how Cohesi...

www.nature.com

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Andi Brunner @andibrunner.bsky.social · Jul 21

Indeed difficult! Only the replicated strands are available for degradation, do ideal would be to BrdU-label in the S-phase prior to fixation. I guess for zebrafish embryos there is no uptake from outside?

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Andi Brunner @andibrunner.bsky.social · Jul 20

BrdU incorporation during S-phase, UV-induced single-strand nicking after cell fixation and Exonuclease based digestion of the nicked DNA strand! :)

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Claas Gefroi @claasgefroi.bsky.social · May 10

"Nicht einen Millimeter überlassen..."
Ich habe das jetzt erst gesehen und was soll ich sagen, was Igor Levit da anlässlich des Todes von Margot Friedländer aus dem Stand heraus sagt, ist das Berührendste, Wahrste und Richtigste, was ich seit ewig im deutschen Fernsehen gehört habe.

Igor Levit über Margot Friedländer beim Deutschen Filmpreis 2025 -

YouTube video by Rederei FM

www.youtube.com

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Laura Rustarazo-Calvo @laura-rustarazo.bsky.social · Mar 27

🎉 Excited to share our new work: “Adhesion-driven tissue rigidification triggers epithelial cell polarity”, now on @biorxivpreprint.bsky.social !

A huge thank you to @nicolettapetridou.bsky.social, Bernat, @crisp-c.bsky.social, Adrián, and everyone involved! 🙌

🧵⤵️

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Deutscher Wetterdienst (DWD) @dwdderwetterdienst.bsky.social · Apr 15

DWD-Fakten zur aktuellen #Trockenheit in #Deutschland: Noch nie seit Beginn der Auswertung 1931 war es in Deutschland im Zeitraum von Anfang Februar bis Mitte April so trocken wie 2025. Mehr Details auch zu #Europa in der heutigen Pressemitteilung unter www.dwd.de/presse

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John Scalzi @scalzi.com · Apr 9

Spoiler: It is absolutely market manipulation

Aaron Rupar @atrupar.com · Apr 9

Rep. Steven Horsford to Greer: "So the trade representative hasn't spoken to the POTUS about a global reordering of trade, but yet he announced it on a tweet? WTF! Who is in charge? It looks like your boss just pulled the rug out from under you. There is no strategy ... is this market manipulation?"

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Ellenberg Lab @ellenberglab.bsky.social · Mar 24

Our new findings on how chromosomes get ready for cell division are now published in @cellpress.bsky.social!

Congratulations, Kai, @andibrunner.bsky.social and everyone else involved! 🤩

www.sciencedirect.com/science/arti...

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EMBL @embl.org · Mar 24

Did you know that DNA must form overlapping loops to give rise to the well-known X-shape of chromosomes? 🧬🧪

New research from EMBL's Ellenberg Group provides new insights into the process of chromosome formation.

@ellenberglab.bsky.social
@andibrunner.bsky.social

www.embl.org/news/science...

How chromosomes shape up for cell division | EMBL

EMBL scientists have shown how overlapping loops of DNA stack upon each other in dividing cells to give rise to rod-shaped chromosomes

www.embl.org

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Andi Brunner @andibrunner.bsky.social · Mar 24

This has been an excellent collaboration together with Kai Beckwith in the @ellenberglab.bsky.social @embl.org. Thanks a lot to Kai, Jan, Natalia, and our collaborator Ralf Jungmann!!

A summary of the work can also be read at the @embl.org press release:
www.embl.org/news/science...

How chromosomes shape up for cell division | EMBL

EMBL scientists have shown how overlapping loops of DNA stack upon each other in dividing cells to give rise to rod-shaped chromosomes

www.embl.org

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Andi Brunner @andibrunner.bsky.social · Mar 24

We thus propose that mitotic chromosome shape is dictated by Condensin-driven extrusion of long and overlapping loops up to a density set by chromatin self-repulsion.

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Andi Brunner @andibrunner.bsky.social · Mar 24

Our model perfectly recapitulated our tracing data & the Condensin localization pattern as quantified recently in Walther et al. 2018.
rupress.org/jcb/article/...

A quantitative map of human Condensins provides new insights into mitotic chromosome architecture | Journal of Cell Biology | Rockefeller University Press

Walther et al. systematically fluorescently tag endogenous Condensin subunits and map their abundance, physical spacing, and mitotic dynamics by fluorescen

rupress.org

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Andi Brunner @andibrunner.bsky.social · Mar 24

Feature 1: Condensins pass each other. This leads to the formation of large, extensively overlapping loops with sizes up to 6-8 Mb.

Feature 2: DNA self-repulsion. This leads to self-organization and stacking of loops into the rod-shape.

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Andi Brunner @andibrunner.bsky.social · Mar 24

Using a dynamic polymer constrained only by available quantitative Condensin chromatin-binding data, we could recapitulate the formation of native mitotic chromosome rods!

Two features were essential for that ->

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Andi Brunner @andibrunner.bsky.social · Mar 24

We found that Condensins are also responsible for the backfolding of DNA at much larger genomic distances: up to 6-8 megabases.

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Andi Brunner @andibrunner.bsky.social · Mar 24

Analyzing these traces, we found evidence for continuously growing loops, and our degron-based experiments showed that these loops are built by the Condensin complexes.

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Andi Brunner @andibrunner.bsky.social · Mar 24

Kai and I used the recently established LoopTrace technology to trace the structure of single mitotic chromosomes in human cells in a very mild & structure pre-serving manner.

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Andi Brunner @andibrunner.bsky.social · Mar 24

Mitotic chromosomes form through long, overlapping and self-repelling DNA loops, generated by the Condensin complexes!

Check out our newest paper here, and see below for a short Bluetorial!
www.cell.com/cell/fulltex...

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Andi Brunner @andibrunner.bsky.social · Mar 24

SO excited to see this out!

EMBL @embl.org · Mar 24

Did you know that DNA must form overlapping loops to give rise to the well-known X-shape of chromosomes? 🧬🧪

New research from EMBL's Ellenberg Group provides new insights into the process of chromosome formation.

@ellenberglab.bsky.social
@andibrunner.bsky.social

www.embl.org/news/science...

How chromosomes shape up for cell division | EMBL

EMBL scientists have shown how overlapping loops of DNA stack upon each other in dividing cells to give rise to rod-shaped chromosomes

www.embl.org

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Ellenberg Lab @ellenberglab.bsky.social · Mar 19

We are #hiring!

We have two open postdoc/research scientist positions to join our #ERC funded project MITOFOLD to resolve the organization of mitotic chromosomes!

1) experimental biologist shorturl.at/LjGEM
2) data scientist shorturl.at/nDdhQ
Please share! 🙌

We look forward to your application! 🤩

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Liz Cheney @lizcheney.bsky.social · Feb 28

History will remember this day— when an American President and Vice President abandoned all we stand for.

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