Control of IRT1 cell surface levels is critical for plant iron uptake from the soil and was previously shown to be regulated by ubiquitin-mediated endocytosis upon non-iron metal excess. Here, we she...

Phytohormone auxin and its directional transport mediate much of the remarkably plastic development of higher plants. Positive feedback between auxin …

The Venus flytrap, a carnivorous plant, catches prey by sensing touch. Here, the authors show that DmMSL10 functions as a high-sensitivity mechanosensor in sensory hairs, initiating long-range calcium and electrical signals required for trap closure.

La pépinière Caussat mise sur des arbres résistants au climat de demain. Leader régional dans la culture d’arbres d’ornement, la pépinière Caussat, située au nord de Toulouse, s’engage dans une course...

Plant ERAD machinery governs the turnover of auxin transporters involved in developmental and stress responses.

Expressing a transport-deficient portion of a calcium transporter protein restores stress sensitivity in plants, revealing a signaling role independent of

Nature Protocols, Published online: 09 September 2025; doi:10.1038/s41596-025-01249-2This protocol presents a versatile approach for the expression and purification of diverse recombinant protein complexes for structural studies, leveraging transient expression in Nicotiana benthamiana leaves to achieve high yield and purity.