Alles Lab
@alleslab.bsky.social
Lab of Dr. Sascha Alles, Associate Professor, Anesthesia, Cincinnati Children's Hospital Medical Center. We study the molecular & cellular physiology of acute and chronic pain. Posts are my own. AllesLab.com
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Alles Lab
@alleslab.bsky.social
· Dec 17
Looks like all members of the @alleslab.bsky.social will be moving! Most of us to Cincy & one of us for a great postdoc opportunity elsewhere. Excited to see great things from each of you! 💪👩🔬🔬 #PainResearch @docnessmonster.bsky.social @goinsyeah.bsky.social @drjones26.bsky.social
Chatting about #painresearch in this recent podcast. Listen here www.scientifica.uk.com/podcasts/a-p...
A Podcast by Neuroverse: Tools for Looking into the Brain -…
Explore what pain really is and why it’s so complex. In this podcast, Prof. Sascha Alles unpacks how chronic pain develops and how the brain processes it.
www.scientifica.uk.com
October 23, 2025 at 10:01 AM
Chatting about #painresearch in this recent podcast. Listen here www.scientifica.uk.com/podcasts/a-p...
Very nice recent study from the Chidambaran Lab in our dept @cincychildrens.bsky.social suggesting potential immune cell signatures in acute vs chronic post-surgical pain #PainResearch pubmed.ncbi.nlm.nih.gov/41077098/
Potential role for immune cell signatures as predictors of acute and chronic pain in adolescents post major musculoskeletal surgery - PubMed
Our findings suggest that immune cell signatures are potentially useful as biomarkers, prognostic indicators, and guides for personalized surgical recovery in youth.
pubmed.ncbi.nlm.nih.gov
October 13, 2025 at 2:18 PM
Very nice recent study from the Chidambaran Lab in our dept @cincychildrens.bsky.social suggesting potential immune cell signatures in acute vs chronic post-surgical pain #PainResearch pubmed.ncbi.nlm.nih.gov/41077098/
Our final contribution to this collaboration is now online. We obtained electrophysiological recordings of DRG/TG neurons and Fos+ neurons of spinal dorsal horn & vlPAG in 2 models of neuropathic pain to study the actions of a humanized P2X4R scFv #PainResearch www.sciencedirect.com/science/arti...
Physiological actions of a humanized P2X4 scFv on peripheral and central neurons in male mice with neuropathic pain
Neuropathic pain remains a challenging clinical condition due to its resistance to conventional analgesics. The purinergic P2X4 receptor (P2X4R), an A…
www.sciencedirect.com
September 17, 2025 at 5:00 PM
Our final contribution to this collaboration is now online. We obtained electrophysiological recordings of DRG/TG neurons and Fos+ neurons of spinal dorsal horn & vlPAG in 2 models of neuropathic pain to study the actions of a humanized P2X4R scFv #PainResearch www.sciencedirect.com/science/arti...
Back in the saddle! #PainResearch #ephys
September 12, 2025 at 7:29 PM
Back in the saddle! #PainResearch #ephys
Reposted by Alles Lab
⚡We are finally electrocuting DRG neurons in the @alleslab.bsky.social since moving to @cincychildrens.bsky.social! #PainResearch #ephys #NeuroSkyence
September 10, 2025 at 5:51 PM
⚡We are finally electrocuting DRG neurons in the @alleslab.bsky.social since moving to @cincychildrens.bsky.social! #PainResearch #ephys #NeuroSkyence
First grant since joining @cincychildrens.bsky.social has been awarded!! Excited to get to work on NIH HEAL Initiative "Early-Stage Discovery of New Pain Targets Within the Understudied Druggable Proteome" with the de la Pena lab!!! #PainResearch
August 28, 2025 at 6:38 PM
First grant since joining @cincychildrens.bsky.social has been awarded!! Excited to get to work on NIH HEAL Initiative "Early-Stage Discovery of New Pain Targets Within the Understudied Druggable Proteome" with the de la Pena lab!!! #PainResearch
One of our final papers from this collaborative effort involving collaborators at UNM, Mayo Clinic, & McGill. Here we validate a humanized scFv targeting hP2XR. Kudos to first-author @docnessmonster.bsky.social & former lab member Mark S. for making this happen! journals.sagepub.com/doi/10.1177/...
Sage Journals: Discover world-class research
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journals.sagepub.com
August 26, 2025 at 12:37 PM
One of our final papers from this collaborative effort involving collaborators at UNM, Mayo Clinic, & McGill. Here we validate a humanized scFv targeting hP2XR. Kudos to first-author @docnessmonster.bsky.social & former lab member Mark S. for making this happen! journals.sagepub.com/doi/10.1177/...
Elegant work highlighting an important aspect of PA activity in sciatic nerve injury-related chronic pain! journals.lww.com/pain/fulltex... #PainResearch
A combined activity of peripheral nociceptive and... : PAIN
eptors. Using electrical and optogenetic approaches, we selectively activated different fiber subtypes without causing neuronal injury. Our findings demonstrate that only simultaneous activation of bo...
journals.lww.com
August 25, 2025 at 1:17 PM
Elegant work highlighting an important aspect of PA activity in sciatic nerve injury-related chronic pain! journals.lww.com/pain/fulltex... #PainResearch
Transporters in pain! Exciting work from @middletonsj.bsky.social & Bennett lab @ox.ac.uk www.nature.com/articles/s41... #PainResearch
SLC45A4 is a pain gene encoding a neuronal polyamine transporter - Nature
The SLC45A4 gene encodes a neuronal polyamine transporter and is linked to pain response in humans and mice.
www.nature.com
August 21, 2025 at 3:06 PM
Transporters in pain! Exciting work from @middletonsj.bsky.social & Bennett lab @ox.ac.uk www.nature.com/articles/s41... #PainResearch
Reposted by Alles Lab
📸 Big day in the lab!
Our new electrophysiology rig is officially up and running — huge thanks to everyone who helped make this happen. Excited for the science ahead! 🧠⚡️
@alleslab.bsky.social @cincychildrens.bsky.social #PainResearch
Our new electrophysiology rig is officially up and running — huge thanks to everyone who helped make this happen. Excited for the science ahead! 🧠⚡️
@alleslab.bsky.social @cincychildrens.bsky.social #PainResearch
August 20, 2025 at 1:51 PM
📸 Big day in the lab!
Our new electrophysiology rig is officially up and running — huge thanks to everyone who helped make this happen. Excited for the science ahead! 🧠⚡️
@alleslab.bsky.social @cincychildrens.bsky.social #PainResearch
Our new electrophysiology rig is officially up and running — huge thanks to everyone who helped make this happen. Excited for the science ahead! 🧠⚡️
@alleslab.bsky.social @cincychildrens.bsky.social #PainResearch
August 19, 2025 at 7:53 PM
Reposted by Alles Lab
I’m proud to share that our first paper from the lab has just been published in Nature Communications: www.nature.com/articles/s41...
This work uncovers a mechanism by which autoantibodies targeting CRMP5 contribute to neuropathic pain in paraneoplastic syndromes.
#PainResearch
This work uncovers a mechanism by which autoantibodies targeting CRMP5 contribute to neuropathic pain in paraneoplastic syndromes.
#PainResearch
Anti-CV2/CRMP5 autoantibodies as drivers of sensory neuron excitability and pain in rats - Nature Communications
Lung and thymoma cancer patients often suffer from autoimmunity and related painful neuropathies. Here the authors show that patient-derived anti-CRMP5 autoantibody binds to rat dorsal root ganglia to...
www.nature.com
August 8, 2025 at 7:36 AM
I’m proud to share that our first paper from the lab has just been published in Nature Communications: www.nature.com/articles/s41...
This work uncovers a mechanism by which autoantibodies targeting CRMP5 contribute to neuropathic pain in paraneoplastic syndromes.
#PainResearch
This work uncovers a mechanism by which autoantibodies targeting CRMP5 contribute to neuropathic pain in paraneoplastic syndromes.
#PainResearch
Thanks for the highlight of our recent work! #PainResearch @painresforum.bsky.social
painresearchforum.org/paper/expres...
painresearchforum.org/paper/expres...
EXPRESS: Gabapentin's effect on human dorsal root ganglia: Donor-specific electrophysiological and transcriptomic profiles - Pain Research Forum
Source: Molecular pain Authors: Jenna B Demeter, Nesia A Zurek, Maddy R Koch, Aleyah E Goins, Cristian O Holguin, Mark W Shilling, Michael S Davis, Reza Ehsanian, Sascha Ra Alles, June Bryan de la Peñ...
painresearchforum.org
July 31, 2025 at 7:27 PM
Thanks for the highlight of our recent work! #PainResearch @painresforum.bsky.social
painresearchforum.org/paper/expres...
painresearchforum.org/paper/expres...
Our collaborative study with the de la Peña lab is now online! Here we analyzed ephys & transcriptomic actions of gabapentin on hDRG neurons. Kudos to co-first authors Jenna & @docnessmonster.bsky.social. Special thks to our donors & their families #PainResearch journals.sagepub.com/doi/10.1177/...
Sage Journals: Discover world-class research
Subscription and open access journals from Sage, the world's leading independent academic publisher.
journals.sagepub.com
July 14, 2025 at 2:39 PM
Our collaborative study with the de la Peña lab is now online! Here we analyzed ephys & transcriptomic actions of gabapentin on hDRG neurons. Kudos to co-first authors Jenna & @docnessmonster.bsky.social. Special thks to our donors & their families #PainResearch journals.sagepub.com/doi/10.1177/...
Now online - our studies of mouse and human sensory neurons in the context of visceral pain! #PainResearch journals.sagepub.com/doi/10.1177/...
Sage Journals: Discover world-class research
Subscription and open access journals from Sage, the world's leading independent academic publisher.
journals.sagepub.com
June 17, 2025 at 2:26 PM
Now online - our studies of mouse and human sensory neurons in the context of visceral pain! #PainResearch journals.sagepub.com/doi/10.1177/...
Reposted by Alles Lab
A read only for the DRG nerd: "Spatial organization, chromatin accessibility and gene-regulatory programs defining mouse sensory neurons". www.nature.com/articles/s42...
Spatial organization, chromatin accessibility and gene-regulatory programs defining mouse sensory neurons - Communications Biology
Single cell RNA-sequencing, spatial transcriptomics and chromatin analyses of sensory neurons provide insights into cell type organization of the dorsal root ganglion and the enhancer-driven co-regula...
www.nature.com
June 13, 2025 at 11:30 AM
A read only for the DRG nerd: "Spatial organization, chromatin accessibility and gene-regulatory programs defining mouse sensory neurons". www.nature.com/articles/s42...
Great week for Nav channel work - this time it's Nav1.9 from the Bosmans lab www.science.org/doi/10.1126/...
A robust expression system reveals distinct gating mechanisms and calmodulin regulation of NaV1.9 channels
An expression system enables detailed study of NaV1.9 channel gating and its regulation by calmodulin.
www.science.org
May 30, 2025 at 12:14 PM
Great week for Nav channel work - this time it's Nav1.9 from the Bosmans lab www.science.org/doi/10.1126/...
Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine pubmed.ncbi.nlm.nih.gov/40424150/ #PainResearch
Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine - PubMed
Nav1.8 voltage-gated sodium channels are strongly expressed in human primary pain-sensing neurons (nociceptors) and a selective Nav1.8 inhibitor VX-548 (suzetrigine) has shown efficacy for treating ac...
pubmed.ncbi.nlm.nih.gov
May 29, 2025 at 5:54 PM
Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine pubmed.ncbi.nlm.nih.gov/40424150/ #PainResearch
Good times at the Cincy Neuroscience Retreat connecting with neuro people @cincychildrens.bsky.social & UC and exploring trails of Ohio! #neuroskyence
May 16, 2025 at 10:36 PM
Good times at the Cincy Neuroscience Retreat connecting with neuro people @cincychildrens.bsky.social & UC and exploring trails of Ohio! #neuroskyence
Lovely preprint with some thought-provoking findings from Diane Lipscombe's group www.biorxiv.org/content/10.1...
Trpv1-dependent Cacna1b gene inactivation reveals cell-specific functions of CaV2.2 channels in vivo
Voltage-gated CaV2.2 channels underlie the N-type current, and they regulate calcium entry at many presynaptic nerve endings to control transmitter release. A role for CaV2.2 channels has been well-es...
www.biorxiv.org
May 12, 2025 at 12:21 PM
Lovely preprint with some thought-provoking findings from Diane Lipscombe's group www.biorxiv.org/content/10.1...
First day! @cincychildrens.bsky.social #PainResearch
April 29, 2025 at 2:56 PM
First day! @cincychildrens.bsky.social #PainResearch
Reposted by Alles Lab
Reposted by Alles Lab
🔬 Exciting discovery! We've been curious about the function of unique IBA1+ cell staining we observed around healthy human DRG neurons in the lab (indicated by red arrows). A new paper @cellpress.bsky.social finally provides some answers. Link: www.cell.com/cell/fulltex...
April 8, 2025 at 2:33 PM
🔬 Exciting discovery! We've been curious about the function of unique IBA1+ cell staining we observed around healthy human DRG neurons in the lab (indicated by red arrows). A new paper @cellpress.bsky.social finally provides some answers. Link: www.cell.com/cell/fulltex...
This is shaping up to be a pretty cool collection of papers! If you're interested in submitting something, give me a shout. #PainResearch www.frontiersin.org/research-top...
Frontiers | Pharmacological Perspectives into Transient Receptor Potential Channels
Transient Receptor Potential (TRP) channels constitute a diverse family of ion channels found in various cell types and tissues throughout the body. These ch...
www.frontiersin.org
April 4, 2025 at 3:27 PM
This is shaping up to be a pretty cool collection of papers! If you're interested in submitting something, give me a shout. #PainResearch www.frontiersin.org/research-top...