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Alles Lab

@alleslab.bsky.social

460 followers 480 following 44 posts

Lab of Dr. Sascha Alles, Associate Professor, Anesthesia, Cincinnati Children's Hospital Medical Center. We study the molecular & cellular physiology of acute and chronic pain. Posts are my own. AllesLab.com

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Alles Lab @alleslab.bsky.social · Dec 17

Looks like all members of the @alleslab.bsky.social will be moving! Most of us to Cincy & one of us for a great postdoc opportunity elsewhere. Excited to see great things from each of you! 💪👩‍🔬🔬 #PainResearch @docnessmonster.bsky.social @goinsyeah.bsky.social @drjones26.bsky.social

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Alles Lab @alleslab.bsky.social · 21d

Our final contribution to this collaboration is now online. We obtained electrophysiological recordings of DRG/TG neurons and Fos+ neurons of spinal dorsal horn & vlPAG in 2 models of neuropathic pain to study the actions of a humanized P2X4R scFv #PainResearch www.sciencedirect.com/science/arti...

Physiological actions of a humanized P2X4 scFv on peripheral and central neurons in male mice with neuropathic pain

Neuropathic pain remains a challenging clinical condition due to its resistance to conventional analgesics. The purinergic P2X4 receptor (P2X4R), an A…

www.sciencedirect.com

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Alles Lab @alleslab.bsky.social · 26d

Back in the saddle! #PainResearch #ephys

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Nesia Zurek, PhD @docnessmonster.bsky.social · 28d

⚡We are finally electrocuting DRG neurons in the @alleslab.bsky.social since moving to @cincychildrens.bsky.social! #PainResearch #ephys #NeuroSkyence

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Alles Lab @alleslab.bsky.social · Aug 28

First grant since joining @cincychildrens.bsky.social has been awarded!! Excited to get to work on NIH HEAL Initiative "Early-Stage Discovery of New Pain Targets Within the Understudied Druggable Proteome" with the de la Pena lab!!! #PainResearch

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Alles Lab @alleslab.bsky.social · Aug 26

One of our final papers from this collaborative effort involving collaborators at UNM, Mayo Clinic, & McGill. Here we validate a humanized scFv targeting hP2XR. Kudos to first-author @docnessmonster.bsky.social & former lab member Mark S. for making this happen! journals.sagepub.com/doi/10.1177/...

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Alles Lab @alleslab.bsky.social · Aug 25

Elegant work highlighting an important aspect of PA activity in sciatic nerve injury-related chronic pain! journals.lww.com/pain/fulltex... #PainResearch

A combined activity of peripheral nociceptive and... : PAIN

eptors. Using electrical and optogenetic approaches, we selectively activated different fiber subtypes without causing neuronal injury. Our findings demonstrate that only simultaneous activation of bo...

journals.lww.com

Alles Lab @alleslab.bsky.social · Aug 21

Transporters in pain! Exciting work from @middletonsj.bsky.social & Bennett lab @ox.ac.uk www.nature.com/articles/s41... #PainResearch

SLC45A4 is a pain gene encoding a neuronal polyamine transporter - Nature

The SLC45A4 gene encodes a neuronal polyamine transporter and is linked to pain response in humans and mice.

www.nature.com

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Reposted by Alles Lab

Nesia Zurek, PhD @docnessmonster.bsky.social · Aug 20

📸 Big day in the lab!
Our new electrophysiology rig is officially up and running — huge thanks to everyone who helped make this happen. Excited for the science ahead! 🧠⚡️
@alleslab.bsky.social @cincychildrens.bsky.social #PainResearch

Three lab members standing proudly in front of a newly assembled electrophysiology rig. The setup includes a micromanipulator, amplifier, and monitor, with cables neatly arranged. Everyone is smiling in casual lab attire, celebrating the milestone in a well-lit lab space.

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Alles Lab @alleslab.bsky.social · Aug 19

Almost ready to rumble! #ephys #PainResearch @cincychildrens.bsky.social

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Aubin Moutal @aubinmoutal.bsky.social · Aug 8

I’m proud to share that our first paper from the lab has just been published in Nature Communications: www.nature.com/articles/s41...
This work uncovers a mechanism by which autoantibodies targeting CRMP5 contribute to neuropathic pain in paraneoplastic syndromes.
#PainResearch

Anti-CV2/CRMP5 autoantibodies as drivers of sensory neuron excitability and pain in rats - Nature Communications

Lung and thymoma cancer patients often suffer from autoimmunity and related painful neuropathies. Here the authors show that patient-derived anti-CRMP5 autoantibody binds to rat dorsal root ganglia to...

www.nature.com

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Alles Lab @alleslab.bsky.social · Jul 31

Thanks for the highlight of our recent work! #PainResearch @painresforum.bsky.social
painresearchforum.org/paper/expres...

EXPRESS: Gabapentin's effect on human dorsal root ganglia: Donor-specific electrophysiological and transcriptomic profiles - Pain Research Forum

Source: Molecular pain Authors: Jenna B Demeter, Nesia A Zurek, Maddy R Koch, Aleyah E Goins, Cristian O Holguin, Mark W Shilling, Michael S Davis, Reza Ehsanian, Sascha Ra Alles, June Bryan de la Peñ...

painresearchforum.org

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Alles Lab @alleslab.bsky.social · Jul 14

Our collaborative study with the de la Peña lab is now online! Here we analyzed ephys & transcriptomic actions of gabapentin on hDRG neurons. Kudos to co-first authors Jenna & @docnessmonster.bsky.social. Special thks to our donors & their families #PainResearch journals.sagepub.com/doi/10.1177/...

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Alles Lab @alleslab.bsky.social · Jun 27

This is really concerning.

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Alles Lab @alleslab.bsky.social · Jun 17

Now online - our studies of mouse and human sensory neurons in the context of visceral pain! #PainResearch journals.sagepub.com/doi/10.1177/...

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Subscription and open access journals from Sage, the world's leading independent academic publisher.

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Patrik Ernfors @patrikernfors.bsky.social · Jun 13

A read only for the DRG nerd: "Spatial organization, chromatin accessibility and gene-regulatory programs defining mouse sensory neurons". www.nature.com/articles/s42...

Spatial organization, chromatin accessibility and gene-regulatory programs defining mouse sensory neurons - Communications Biology

Single cell RNA-sequencing, spatial transcriptomics and chromatin analyses of sensory neurons provide insights into cell type organization of the dorsal root ganglion and the enhancer-driven co-regula...

www.nature.com

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Alles Lab @alleslab.bsky.social · May 30

Great week for Nav channel work - this time it's Nav1.9 from the Bosmans lab www.science.org/doi/10.1126/...

A robust expression system reveals distinct gating mechanisms and calmodulin regulation of NaV1.9 channels

An expression system enables detailed study of NaV1.9 channel gating and its regulation by calmodulin.

www.science.org

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Alles Lab @alleslab.bsky.social · May 29

Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine pubmed.ncbi.nlm.nih.gov/40424150/ #PainResearch

Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine - PubMed

Nav1.8 voltage-gated sodium channels are strongly expressed in human primary pain-sensing neurons (nociceptors) and a selective Nav1.8 inhibitor VX-548 (suzetrigine) has shown efficacy for treating ac...

pubmed.ncbi.nlm.nih.gov

Alles Lab @alleslab.bsky.social · May 25

Lab outing for a Reds game and we beat the Cubs... go @reds.com!

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Alles Lab @alleslab.bsky.social · May 16

Good times at the Cincy Neuroscience Retreat connecting with neuro people @cincychildrens.bsky.social & UC and exploring trails of Ohio! #neuroskyence

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Alles Lab @alleslab.bsky.social · May 12

Lovely preprint with some thought-provoking findings from Diane Lipscombe's group www.biorxiv.org/content/10.1...

Trpv1-dependent Cacna1b gene inactivation reveals cell-specific functions of CaV2.2 channels in vivo

Voltage-gated CaV2.2 channels underlie the N-type current, and they regulate calcium entry at many presynaptic nerve endings to control transmitter release. A role for CaV2.2 channels has been well-es...

www.biorxiv.org

Alles Lab @alleslab.bsky.social · Apr 29

First day! @cincychildrens.bsky.social #PainResearch

Reposted by Alles Lab

Sergiu P. Pasca @sergiuppasca.bsky.social · Apr 9

www.nature.com/articles/s41...

Human assembloid model of the ascending neural sensory pathway - Nature

A human ascending somatosensory assembloid model was developed, which integrates multiple organoids to simulate the spinothalamic pathway, demonstrating functional connectivity and responsiveness to s...

www.nature.com

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tBerta Lab @tbertalab.bsky.social · Apr 8

🔬 Exciting discovery! We've been curious about the function of unique IBA1+ cell staining we observed around healthy human DRG neurons in the lab (indicated by red arrows). A new paper @cellpress.bsky.social finally provides some answers. Link: www.cell.com/cell/fulltex...

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Alles Lab @alleslab.bsky.social · Apr 8

Woah 🤯

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Alles Lab @alleslab.bsky.social · Apr 7

Very cool! Congrats Greg and team!