#AskZebrafish
@askzebrafish.bsky.social
Investigating questions/queries from the #zebrafish community! Affiliated with @zebrafishrock.bsky.social 🐟🎸
🤔 #AskZebrafish: Does anyone have experience extracting (tissue-specific) cell monolayers for culture from ZF embryos? Looking for a contact to pick their brain.
November 24, 2025 at 6:20 PM
🤔 #AskZebrafish: Does anyone have experience extracting (tissue-specific) cell monolayers for culture from ZF embryos? Looking for a contact to pick their brain.
🤔 #AskZebrafish: "Do any of you have a protocol or a kit recommendation to do subcellular fractionation of zebrafish embryos. Ideally I'd like to separate the mitochondria and the nucleus (and as a bonus the rest of the cell parts)"
November 18, 2025 at 4:09 PM
🤔 #AskZebrafish: "Do any of you have a protocol or a kit recommendation to do subcellular fractionation of zebrafish embryos. Ideally I'd like to separate the mitochondria and the nucleus (and as a bonus the rest of the cell parts)"
🤔 #AskZebrafish: “Wondering if anyone has recommendations on a relatively inexpensive device to measure water flow rates in a swim tunnel?”
November 15, 2025 at 3:25 PM
🤔 #AskZebrafish: “Wondering if anyone has recommendations on a relatively inexpensive device to measure water flow rates in a swim tunnel?”
#AskZebrafish from Slack: What is the best promoter to use to specifically tag microglia in #zebrafish? Thinking of using mpeg1.1 but just wanted to double check if there are any better options?
November 14, 2025 at 7:33 PM
#AskZebrafish from Slack: What is the best promoter to use to specifically tag microglia in #zebrafish? Thinking of using mpeg1.1 but just wanted to double check if there are any better options?
Query from Slack: Looking for Tg(pax3:GAL4) and any driver line for ascl1:GAL4 or Cre? Preferably in Europe. Any help and/or leads would be appreciated! #AskZebrafish
November 13, 2025 at 2:02 PM
Query from Slack: Looking for Tg(pax3:GAL4) and any driver line for ascl1:GAL4 or Cre? Preferably in Europe. Any help and/or leads would be appreciated! #AskZebrafish
🤔 #AskZebrafish: Looking for a zebrafish version of the Mito-RiboTag mouse line. Has anyone made one or might have a vector they could share? Any leads would be thoroughly appreciated!
November 10, 2025 at 10:07 AM
🤔 #AskZebrafish: Looking for a zebrafish version of the Mito-RiboTag mouse line. Has anyone made one or might have a vector they could share? Any leads would be thoroughly appreciated!
Question from Slack: Looking for antibodies against mscarlet-i that people have good experience with? #AskZebrafish
November 7, 2025 at 9:10 AM
Question from Slack: Looking for antibodies against mscarlet-i that people have good experience with? #AskZebrafish
Query from Slack: Looking for notch2 mutant zebrafish and someone willing to share them? Preferably in the US. 🔍 #AskZebrafish
November 6, 2025 at 8:15 PM
Query from Slack: Looking for notch2 mutant zebrafish and someone willing to share them? Preferably in the US. 🔍 #AskZebrafish
#AskZebrafish query from Slack: Looking for contact that has recently shipped transgenic eggs from Europe to the US. Re: any hurdles you or your lab had to go through. If this describes you or someone you know, please reach out 🙏
November 6, 2025 at 8:47 AM
#AskZebrafish query from Slack: Looking for contact that has recently shipped transgenic eggs from Europe to the US. Re: any hurdles you or your lab had to go through. If this describes you or someone you know, please reach out 🙏
Reposted by #AskZebrafish
Initial trawl came up empty 🤷♂️ Any leads or feedback appreciated!
🤔 #AskZebrafish from Slack: "Does anyone have a favorite protocol for staining myelin in the brain, especially in fish >21 dpf? We would like a dye, not a line. We're looking into CMC or fluoromyelin but are unsure about penetration into the brain." Any feedback or leads would be appreciated!
November 4, 2025 at 8:35 PM
Initial trawl came up empty 🤷♂️ Any leads or feedback appreciated!
Query from Slack: “I was wondering if anyone has a spi1b (pu.1) HCR probe that looks good in whole mount [zebrafish] tissues, the one I designed has a lot of pesky background in the embryo.” #AskZebrafish
November 4, 2025 at 8:38 PM
Query from Slack: “I was wondering if anyone has a spi1b (pu.1) HCR probe that looks good in whole mount [zebrafish] tissues, the one I designed has a lot of pesky background in the embryo.” #AskZebrafish
Query from Slack: Has anyone successfully used a c-fos antibody for whole mount immuostaining in zebrafish? #AskZebrafish 🤔
November 3, 2025 at 7:53 PM
Query from Slack: Has anyone successfully used a c-fos antibody for whole mount immuostaining in zebrafish? #AskZebrafish 🤔
Two queries from Slack:
Anyone know of any good antibodies that work well in whole mount 5-7dpf for labeling mitochondria?
Does anyone in the community work with mitochondria based assays for larval zebrafish? Such as Alamar blue or JC10? Or anything else?
Any leads appreciated!
#AskZebrafish
Anyone know of any good antibodies that work well in whole mount 5-7dpf for labeling mitochondria?
Does anyone in the community work with mitochondria based assays for larval zebrafish? Such as Alamar blue or JC10? Or anything else?
Any leads appreciated!
#AskZebrafish
November 2, 2025 at 3:18 PM
Two queries from Slack:
Anyone know of any good antibodies that work well in whole mount 5-7dpf for labeling mitochondria?
Does anyone in the community work with mitochondria based assays for larval zebrafish? Such as Alamar blue or JC10? Or anything else?
Any leads appreciated!
#AskZebrafish
Anyone know of any good antibodies that work well in whole mount 5-7dpf for labeling mitochondria?
Does anyone in the community work with mitochondria based assays for larval zebrafish? Such as Alamar blue or JC10? Or anything else?
Any leads appreciated!
#AskZebrafish
🤔 #AskZebrafish from Slack: "Does anyone have a favorite protocol for staining myelin in the brain, especially in fish >21 dpf? We would like a dye, not a line. We're looking into CMC or fluoromyelin but are unsure about penetration into the brain." Any feedback or leads would be appreciated!
October 31, 2025 at 3:06 PM
🤔 #AskZebrafish from Slack: "Does anyone have a favorite protocol for staining myelin in the brain, especially in fish >21 dpf? We would like a dye, not a line. We're looking into CMC or fluoromyelin but are unsure about penetration into the brain." Any feedback or leads would be appreciated!
Reposted by #AskZebrafish
Does anyone in Europe have Tg(UAS:iGluSnFR) and Tg(UAS:GCaMP) zebrafish lines available? Looking to share or obtain embryos if possible. Thanks in advance! 🐟🔬
@zebrafishrock.bsky.social
@zebrafishrock.bsky.social
October 30, 2025 at 3:50 PM
#AskZebrafish from Slack: For folks studying metabolism, have you done chemical screens in recent years? If so, which libraries will you recommend? 🤔
October 24, 2025 at 4:17 PM
#AskZebrafish from Slack: For folks studying metabolism, have you done chemical screens in recent years? If so, which libraries will you recommend? 🤔
#AskZebrafish: Does anyone know of a good antibody recognizing mTagBFP2? Preferably available in North America. Any help would be appreciated 🙏
October 23, 2025 at 9:10 AM
#AskZebrafish: Does anyone know of a good antibody recognizing mTagBFP2? Preferably available in North America. Any help would be appreciated 🙏
Query from Slack: Looking for any transgenic lines that have integrated and expressed Cas9. Preferably in the US. 🤔 #AskZebrafish
October 15, 2025 at 5:39 PM
Query from Slack: Looking for any transgenic lines that have integrated and expressed Cas9. Preferably in the US. 🤔 #AskZebrafish
Query from Slack: Looking for a line that labels the nuclei of basal epithelial (and migratory) cells. Preferably in Europe. The best line seems to be one the Sagasti Lab made: p63[BAC]:Gal4 crossed to a line for expression in the nucleus (e.g., UAS:nls-GFP). Would consider others. 🙏 #AskZebrafish
October 15, 2025 at 7:19 AM
Query from Slack: Looking for a line that labels the nuclei of basal epithelial (and migratory) cells. Preferably in Europe. The best line seems to be one the Sagasti Lab made: p63[BAC]:Gal4 crossed to a line for expression in the nucleus (e.g., UAS:nls-GFP). Would consider others. 🙏 #AskZebrafish
Query from Slack: Does anyone know of good commercial centromere and/or kinetochore antibodies that work well in zebrafish? #AskZebrafish
October 13, 2025 at 4:41 PM
Query from Slack: Does anyone know of good commercial centromere and/or kinetochore antibodies that work well in zebrafish? #AskZebrafish
Brainbuster from Slack: Has anyone done agilent seahorse mito stress test on #zebrafish embryos (2-3dpf) and be willing to share a protocol? #AskZebrafish
October 13, 2025 at 4:38 PM
Brainbuster from Slack: Has anyone done agilent seahorse mito stress test on #zebrafish embryos (2-3dpf) and be willing to share a protocol? #AskZebrafish
Query from Slack: Does anyone have an anti-S100b antibody that they are successfully using for IHC in whole-mount larval brains. The one most commonly used one (Z0311 by Dako) seems to have been discontinued. Any pointers to a good one to use in fish would very helpful! #AskZebrafish
October 13, 2025 at 4:26 PM
Query from Slack: Does anyone have an anti-S100b antibody that they are successfully using for IHC in whole-mount larval brains. The one most commonly used one (Z0311 by Dako) seems to have been discontinued. Any pointers to a good one to use in fish would very helpful! #AskZebrafish
Query from Slack: Looking for someone to share somitabun mutants in Europe? Any leads would be appreciated! #AskZebrafish
October 9, 2025 at 4:37 PM
Query from Slack: Looking for someone to share somitabun mutants in Europe? Any leads would be appreciated! #AskZebrafish
Query from Slack: Looking for people with experience in proximity labelling in zebrafish? Considering using miniTurbo instead of BioID or APEX, what do the community think of it? Also looking for anybody that has experience with targeting labeling enzymes to the ER/secretory pathway? #AskZebrafish
October 9, 2025 at 4:35 PM
Query from Slack: Looking for people with experience in proximity labelling in zebrafish? Considering using miniTurbo instead of BioID or APEX, what do the community think of it? Also looking for anybody that has experience with targeting labeling enzymes to the ER/secretory pathway? #AskZebrafish
🤔 #AskZebrafish: Would anyone within the EU have a mutant ybx1 line swimming around and willing to share? Any leads would be appreciated.
October 2, 2025 at 6:38 PM
🤔 #AskZebrafish: Would anyone within the EU have a mutant ybx1 line swimming around and willing to share? Any leads would be appreciated.