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Ask Zebrafish? @askzebrafish.bsky.social · 1d

Query from Slack: Looking for someone to share somitabun mutants in Europe? Any leads would be appreciated! #AskZebrafish

Ask Zebrafish? @askzebrafish.bsky.social · 1d

Query from Slack: Looking for people with experience in proximity labelling in zebrafish? Considering using miniTurbo instead of BioID or APEX, what do the community think of it? Also looking for anybody that has experience with targeting labeling enzymes to the ER/secretory pathway? #AskZebrafish

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Ask Zebrafish? @askzebrafish.bsky.social · 8d

🤔 #AskZebrafish: Would anyone within the EU have a mutant ybx1 line swimming around and willing to share? Any leads would be appreciated.

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Ask Zebrafish? @askzebrafish.bsky.social · 8d

🤔 #AskZebrafish: Looking for krt18a reporter fish in the UK or if anyone has a ready to inject construct for making one and are willing to share, that would be much appreciated!

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Ask Zebrafish? @askzebrafish.bsky.social · 8d

🤔 #AskZebrafish: Looking for Prom1a and/or Prom1b mutant lines and/or reporter lines that someone would be willing to share? Preferably in the USA.

Ask Zebrafish? @askzebrafish.bsky.social · Jun 20

🤔 Query from Slack: Looking for 5' myl7/cmlc2 promoter vector and someone willing to share it? Preferably in the US 🐟🫀 #AskZebrafish

Ask Zebrafish? @askzebrafish.bsky.social · Jun 9

Another query from Slack: Looking for ccdc40 mutant #zebrafish (aka locke). Preferably in the US. Any leads would be much appreciated! #AskZebrafish

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Ask Zebrafish? @askzebrafish.bsky.social · Jun 9

Query from Slack: Does anyone have good recommendations for antibodies that label mitochondrial DNA encoded proteins and work well in #zebrafish? #AskZebrafish

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Ask Zebrafish? @askzebrafish.bsky.social · May 15

Anonymous query from Slack: Does anyone have the TgBAC(foxp3a:TagCFP-NTR)vcc5 line swimming around, preferably in the US? Thanks in advance! #AskZebrafish 🤔

Ask Zebrafish? @askzebrafish.bsky.social · May 13

Still on the hunt for fish lines 🙏

Zebrafish Rock! @zebrafishrock.bsky.social · May 9

🤔 #AskZebrafish: Looking for a transgenic line that labels cranial muscles, preferably in the USA? #zebrafish

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Ask Zebrafish? @askzebrafish.bsky.social · May 1

Still searching for leads! 🙏

An image of a spotlight with a zebrafish during a dark, rainy night. Akin to the bat signal.

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Reposted by Ask Zebrafish?

Zebrafish Rock! @zebrafishrock.bsky.social · Apr 29

Head-scratcher from Slack: Does anyone know if it is possible to tell (by eye/microscope) if the developing oocytes in a zebrafish ovary are at the pre-, during, or post-vitellogenesis stage? 🤔 #AskZebrafish

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Reposted by Ask Zebrafish?

Zebrafish Rock! @zebrafishrock.bsky.social · Apr 26

Looking for anyone w/ experience in smRNA analysis who can provide someone with information or guidance? They already have their sequences, but can't find a clear methodology/protocol to help them analyze it. The sequences were obtained from NOVOGENE's Small RNA Sequencing service. 🤔 #AskZebrafish

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Ask Zebrafish? @askzebrafish.bsky.social · Apr 22

Any leads would be appreciated 🙏

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Ask Zebrafish? @askzebrafish.bsky.social · Apr 8

Any leads would be appreciated! Reposts welcome 🙏

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Ask Zebrafish? @askzebrafish.bsky.social · Apr 7

Still searching for leads 👀

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Ask Zebrafish? @askzebrafish.bsky.social · Apr 4

Still looking for leads 🕵️

Reposted by Ask Zebrafish?

Zebrafish Rock! @zebrafishrock.bsky.social · Apr 4

Query from our Slack Tankspace: Does anyone in Europe have the Tg(mpx: mcherry ) line from the Huttenlocher Lab or similar and would be willing to share? #AskZebrafish

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Ask Zebrafish? @askzebrafish.bsky.social · Apr 4

Still looking for leads 🕵️

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Ask Zebrafish? @askzebrafish.bsky.social · Mar 31

Re-upping

Zebrafish Rock! @zebrafishrock.bsky.social · Mar 18

Query from Slack: Does anyone have scn8aa and scn8ab #zebrafish mutants and would be willing to share some embryos? (Preferably in Europe, if possible) 🔎 #AskZebrafish

Ask Zebrafish? @askzebrafish.bsky.social · Mar 31

Ping @bazzinilab.bsky.social Didn’t your team do something like this?

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Ask Zebrafish? @askzebrafish.bsky.social · Mar 31

Did you find this yet?

Reposted by Ask Zebrafish?

Zebrafish Rock! @zebrafishrock.bsky.social · Mar 27

Another query from Slack: Does anyone (preferably in the US) have the Tg(GFAP:iGluSnFR) line and be willing to send them to another #zebrafish lab? 🤔 #AskZebrafish

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Ask Zebrafish? @askzebrafish.bsky.social · Mar 27

🤔 #AskZebrafish

Zebrafish Rock! @zebrafishrock.bsky.social · Mar 27

Query from Slack: Does anyone have a plasmid construct with a dominant negative form of protein kinase A (PKA) or experience using it with zebrafish embryos? Any help and/or leads would be appreciated!

Ask Zebrafish? @askzebrafish.bsky.social · Mar 21

Thanks but we don’t think this would be classified as ‘efficiently’