Jamie Walters
@bioboatr.bsky.social
600 followers 610 following 180 posts
Professor, University of Kansas, Ecology & Evolutionary Biology Dept. Researching evolutionary genomics in Lepidoptera.
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Reposted by Jamie Walters
juliusbrennecke.bsky.social
PIWI clade Argonautes are essential for transposon silencing. Without them, animals are sterile due to massive transposon activity.

But how does piRNA-guided target interaction translate into silencing?

PhD student Júlia Portell Montserrat has an intriguing answer

www.cell.com/molecular-ce...
Reposted by Jamie Walters
tomsaunders.me
The Art of Data Visualization with ggplot2: A free online book by @nrennie.bsky.social that guides us through the entire process of creating plots, including why certain decisions were made, using real datasets that have been part of #TidyTuesday. Very excited to get stuck into this one. #rstats
The Art of Data Visualization with ggplot2
The TidyTuesday Cookbook
nrennie.rbind.io
Reposted by Jamie Walters
Reposted by Jamie Walters
johnlogsdon.bsky.social
1) Are you an Assistant Professor studying evolutionary biology?

2) Are you in the US?

3) Would you like to give a research seminar in Iowa?

4) Are you available to travel September 4-6?

If you can answer YES x4, please DM me ASAP, or comment below.
Reposted by Jamie Walters
evolvwing.bsky.social
Transgenic Plodia larvae expressing the very bright mBaoJin (mStayGold variant) in its silk glands.

Also some gorgeous 3xP3:mCherry staining of larval ocelli and glia.

(see revised preprint) by pantry moth wizards
@donyaniyaz.bsky.social & @lucalivraghi.bsky.social doi.org/10.1101/2025...
bioboatr.bsky.social
Do orally secreted REPATs to belong to a specific clade? use proteomics on saliva. Most proteins found in paralogous clades.

SelV1 infection reduces the abundance of some REPATS. SelV1 infection modulates plant response to caterpillars.
bioboatr.bsky.social
First perform manual annotations and phylogeny. MBF2 domain is absent in clade in Spodoptera only.
two clades: "one to one" and another clade "many to many" with extensive paralogs.
bioboatr.bsky.social
But many REPAT proteins were initially found in REsponse to PAThothens.
So REPATS are important in insect-plant and insect-pathogen interactions. Is there a 3-way interaction here?
bioboatr.bsky.social
Effector proteins from saliva interact with plants to suppress defense. A recently discovered class include REPAT.
REPAT are co-transcription factors, enhance gene action that suppress Jasmonic Acid pathways.
bioboatr.bsky.social
**REPAT proteins as mediators of pathogen-insect-plant interactions in Spodoptera exigua**
Cristina Crava, Universitat de Valencia

Plants have direct and indirect defenses to insect herbivory.
bioboatr.bsky.social
Gut microbiome overwhelmingly enterococcus. different bacterial enterococcus species are variably pathogenic or beneficial.
kmo-KO have worse adaptation to pepper plant, Higher susceptibility to other pathogens.
bioboatr.bsky.social
Examine bacterial composition via 16s on fore,mid,hind-gut.
kmo-KO have much less diversity in fore & mid, but not hind. Consistent with observation for more HQA in fore & mid gut.
bioboatr.bsky.social
8'-HQA, produced by moth, iron chelator. Primarily found in gut.
Target CRISPR at kmo gene, knocked out production of 8'-HQA. KO individuals have orange eyes, not brown.
bioboatr.bsky.social
**Kynurenine 3-monooxygenase disruption by CRISPR/Cas9 in Spodoptera exigua: phenotype, microbial diversity, and fitness costs**
Daniel Pinos, Universitat de Valencia
#lepgencrete

Focus on plant-insect-pathogen interactions. Some bacteria produce important/required compounds for host.
bioboatr.bsky.social
Considering more recent climates, and predicting future, suggests expansion northward even into southern UK. But also largely absent from Africa, in souther portion of range.
bioboatr.bsky.social
Can then use model to find limiting climatic factors. Suggests max summer temp is limiting to south (into Africa), but in winter, thermal radition & min temps limited northward range.
bioboatr.bsky.social
Deploy species distribution modeling, focusing on
- minimum winter temperature.
- Downward thermal radiation in winter
Analyzed climatic data 1960-1980. Model of suitability fits well the historical distribution before 1980.
bioboatr.bsky.social
Historically ranged in southern europe, to middle France, but is expanding northward notably in the last 15 years, closely following increases in minimum winter temperature.
bioboatr.bsky.social
Eggs and early larvae in summer, larvale development fall-winter, come February larvae "process" in head-tail lines, to find pupation sites underground.
bioboatr.bsky.social
**A climate change winner: How warmer and brighter winters trigger the expansion of the pine processionary moth**
Jean-Pierre Rossi, CBGP, INRAE, Montpelier, France
#lepgenecrete

Pine processionary moth produces urticating hairs producing strong allergic reactions in humans and other animals.
bioboatr.bsky.social
Also investigating medicinal capacity of butterfly detoxification of carcinogenic aristolochic acids.
bioboatr.bsky.social
Research efforts also include developing machine learning (SVMorph tool) for complex morphological classification, and deep learning for genetic introgression (ERICA tool).