Chi-Min Ho
@cmholab.bsky.social
Structural Biologist • Biochemist • Parasitologist • Co-host of ThePlungePodcast theplunge.org • Assistant Professor @ Columbia • Cryo electron microscopy/tomography of malarial membrane protein complexes. #teamtomo #cryoET #cryoEM #malaria cmholab.org
Reposted by Chi-Min Ho
Mechanism of Ribosome Stalling by the AMD1 C-terminal Tail Arrest Peptide https://www.biorxiv.org/content/10.1101/2025.11.16.688537v1
November 17, 2025 at 12:50 AM
Mechanism of Ribosome Stalling by the AMD1 C-terminal Tail Arrest Peptide https://www.biorxiv.org/content/10.1101/2025.11.16.688537v1
Reposted by Chi-Min Ho
New work from Tahirov x Lim collaboration! @qixianghe.bsky.social from my lab contributed the cryo-EM structures for this work. We are excited to help explain how anti-HSV drugs work and to guide their future development.
www.science.org/doi/10.1126/...
@unmc.bsky.social @uwbiochem.bsky.social
www.science.org/doi/10.1126/...
@unmc.bsky.social @uwbiochem.bsky.social
Structural basis of herpesvirus helicase-primase inhibition by pritelivir and amenamevir
Structural studies of HSV-1 helicase-primase revealed how pritelivir and amenamevir bind and block its helicase activity.
www.science.org
November 10, 2025 at 12:14 AM
New work from Tahirov x Lim collaboration! @qixianghe.bsky.social from my lab contributed the cryo-EM structures for this work. We are excited to help explain how anti-HSV drugs work and to guide their future development.
www.science.org/doi/10.1126/...
@unmc.bsky.social @uwbiochem.bsky.social
www.science.org/doi/10.1126/...
@unmc.bsky.social @uwbiochem.bsky.social
Reposted by Chi-Min Ho
Seeing is believing-Plasmodium falciparum translation in action pubmed.ncbi.nlm.nih.gov/41202797/ #cryoem
November 8, 2025 at 9:58 PM
Seeing is believing-Plasmodium falciparum translation in action pubmed.ncbi.nlm.nih.gov/41202797/ #cryoem
Reposted by Chi-Min Ho
Excited to share our latest @nature.com: How does naloxone (Narcan) stop an opioid overdose? We determined the first GDP-bound μ-opioid receptor–G protein structures and found naloxone traps a novel "latent” state, preventing GDP release and G protein activation.💊🧪 🧵👇 www.nature.com/articles/s41...
November 5, 2025 at 4:23 PM
Excited to share our latest @nature.com: How does naloxone (Narcan) stop an opioid overdose? We determined the first GDP-bound μ-opioid receptor–G protein structures and found naloxone traps a novel "latent” state, preventing GDP release and G protein activation.💊🧪 🧵👇 www.nature.com/articles/s41...
Reposted by Chi-Min Ho
I love AlphaFold—but please include PAE (Predicted Aligned Error) plots for every “interaction.” Pretty PDBs ≠ proof. If the PAE doesn’t show an interface, it ain’t one. Show the plots. Structural biology's reputation is on the line.
October 24, 2025 at 5:44 PM
I love AlphaFold—but please include PAE (Predicted Aligned Error) plots for every “interaction.” Pretty PDBs ≠ proof. If the PAE doesn’t show an interface, it ain’t one. Show the plots. Structural biology's reputation is on the line.
Congrats Paul!! Excited to see this out! 🥳
October 28, 2025 at 11:01 PM
Congrats Paul!! Excited to see this out! 🥳
Reposted by Chi-Min Ho
Thrilled our study on heme crystal motion in #malaria @parasitesrule.org is now published @pnas.org, support from NIH, @bwfund.bsky.social, @uofutahcihd.bsky.social, UofU 3i. With Fu and Bidone labs @price.utah.edu, @uofubiochem.bsky.social, @uofuhealth.bsky.social
www.pnas.org/doi/10.1073/...
www.pnas.org/doi/10.1073/...
Chemical propulsion of hemozoin crystal motion in malaria parasites | PNAS
Malaria parasites infect red blood cells where they digest host hemoglobin and release
free heme inside a lysosome-like organelle called the food v...
www.pnas.org
October 28, 2025 at 7:55 PM
Thrilled our study on heme crystal motion in #malaria @parasitesrule.org is now published @pnas.org, support from NIH, @bwfund.bsky.social, @uofutahcihd.bsky.social, UofU 3i. With Fu and Bidone labs @price.utah.edu, @uofubiochem.bsky.social, @uofuhealth.bsky.social
www.pnas.org/doi/10.1073/...
www.pnas.org/doi/10.1073/...
Congrats Misha, looks gorgeous! 🥳Can't wait to dig in 😍
October 28, 2025 at 10:48 PM
Congrats Misha, looks gorgeous! 🥳Can't wait to dig in 😍
Reposted by Chi-Min Ho
We have a new preprint for you. Naked mole rats are very long-lived rodents and have increased translational fidelity.
Interestingly, 28S ribosomal RNA is split. Here we investigated the structure of the ribosome by single particle cryo-EM. Take a look.
www.biorxiv.org/content/10.1...
Interestingly, 28S ribosomal RNA is split. Here we investigated the structure of the ribosome by single particle cryo-EM. Take a look.
www.biorxiv.org/content/10.1...
Cryo-EM structure of the naked mole-rat ribosome reveals a stabilized split 28S rRNA
The naked mole-rat (Heterocephalus glaber) is a long-lived mammal with remarkable resistance to cancer and hypoxia, suggesting the evolution of robust proteostasis networks. The ribosome, the central ...
www.biorxiv.org
October 28, 2025 at 3:24 PM
We have a new preprint for you. Naked mole rats are very long-lived rodents and have increased translational fidelity.
Interestingly, 28S ribosomal RNA is split. Here we investigated the structure of the ribosome by single particle cryo-EM. Take a look.
www.biorxiv.org/content/10.1...
Interestingly, 28S ribosomal RNA is split. Here we investigated the structure of the ribosome by single particle cryo-EM. Take a look.
www.biorxiv.org/content/10.1...
Learn how our hunt for the native structure of top antimalarial target PfATP4🧂led to the discovery of PfABP, an unknown essential binding partner, out now! @natcomms.nature.com 👉 rdcu.be/eLRlH 🦠🔬❄️
A team effort led by @mehsehret.bsky.social & Anurag Shukla @akhilvaidya.bsky.social! #cryoEM #malaria
A team effort led by @mehsehret.bsky.social & Anurag Shukla @akhilvaidya.bsky.social! #cryoEM #malaria
Endogenous structure of antimalarial target PfATP4 reveals an apicomplexan-specific P-type ATPase modulator
Nature Communications - Here, the authors present the 3.7 Å cryoEM structure of native sodium efflux pump PfATP4 from Plasmodium falciparum, revealing a bound protein that they term...
rdcu.be
October 20, 2025 at 12:55 PM
Learn how our hunt for the native structure of top antimalarial target PfATP4🧂led to the discovery of PfABP, an unknown essential binding partner, out now! @natcomms.nature.com 👉 rdcu.be/eLRlH 🦠🔬❄️
A team effort led by @mehsehret.bsky.social & Anurag Shukla @akhilvaidya.bsky.social! #cryoEM #malaria
A team effort led by @mehsehret.bsky.social & Anurag Shukla @akhilvaidya.bsky.social! #cryoEM #malaria
Reposted by Chi-Min Ho
It was so fun to work on this with @gkocker.bsky.social and @clienkaemper.bsky.social! It is out @pnas.org
If you love olfaction and love math, this one is for you!
🦟👃🧮
When noncanonical olfaction is optimal | PNAS www.pnas.org/doi/10.1073/...
If you love olfaction and love math, this one is for you!
🦟👃🧮
When noncanonical olfaction is optimal | PNAS www.pnas.org/doi/10.1073/...
When noncanonical olfaction is optimal | PNAS
The early olfactory system is canonically described by a “one-receptor-to-one-neuron”
model: each olfactory sensory neuron (OSN) expresses a single...
www.pnas.org
October 8, 2025 at 12:42 PM
It was so fun to work on this with @gkocker.bsky.social and @clienkaemper.bsky.social! It is out @pnas.org
If you love olfaction and love math, this one is for you!
🦟👃🧮
When noncanonical olfaction is optimal | PNAS www.pnas.org/doi/10.1073/...
If you love olfaction and love math, this one is for you!
🦟👃🧮
When noncanonical olfaction is optimal | PNAS www.pnas.org/doi/10.1073/...
Congrats, Halil and Team! 🥳
September 30, 2025 at 10:22 PM
Congrats, Halil and Team! 🥳
Reposted by Chi-Min Ho
Excited to share that our paper on mitochondrial cardiolipin dynamics is published in @natcomms.nature.com today! We provide molecular explanations for unique aspects of mitochondrial morphology and the mechanisms underlying cardiolipin-related Barth Syndrome.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Cardiolipin dynamics promote membrane remodeling by mitochondrial OPA1 - Nature Communications
This study reveals how cardiolipin governs mitochondrial morphology by modulating the activity of human OPA1 and how its replacement by monolyso-cardiolipin, as observed in Barth syndrome, impacts mitochondrial membrane-shaping mechanisms.
www.nature.com
September 30, 2025 at 8:09 PM
Excited to share that our paper on mitochondrial cardiolipin dynamics is published in @natcomms.nature.com today! We provide molecular explanations for unique aspects of mitochondrial morphology and the mechanisms underlying cardiolipin-related Barth Syndrome.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Chi-Min Ho
Behind every scientist is an army of workers who keep the biomedical research ecosystem running. Hear from these workers and how they contribute at www.nycures.org/ and SIGN UP to have your voice heard!
September 25, 2025 at 12:16 AM
Behind every scientist is an army of workers who keep the biomedical research ecosystem running. Hear from these workers and how they contribute at www.nycures.org/ and SIGN UP to have your voice heard!
Reposted by Chi-Min Ho
We have a preprint of a book chapter for you: Structural Analysis of Membrane Proteins in Cell-Derived Microvesicles.
We describe how to image membrane proteins in mid-sized vesicles using cryo-electron tomography
zenodo.org/records/1717...
We describe how to image membrane proteins in mid-sized vesicles using cryo-electron tomography
zenodo.org/records/1717...
Structural Analysis of Membrane Proteins in Cell-Derived Microvesicles
zenodo.org
September 22, 2025 at 12:36 PM
We have a preprint of a book chapter for you: Structural Analysis of Membrane Proteins in Cell-Derived Microvesicles.
We describe how to image membrane proteins in mid-sized vesicles using cryo-electron tomography
zenodo.org/records/1717...
We describe how to image membrane proteins in mid-sized vesicles using cryo-electron tomography
zenodo.org/records/1717...
Reposted by Chi-Min Ho
🚀 Check out our new preprint on extending the scope of single-particle cryo-EM with 2D template matching!
Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching is now available on bioRxiv!
www.biorxiv.org/content/10.1...
Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching is now available on bioRxiv!
www.biorxiv.org/content/10.1...
September 17, 2025 at 5:07 PM
🚀 Check out our new preprint on extending the scope of single-particle cryo-EM with 2D template matching!
Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching is now available on bioRxiv!
www.biorxiv.org/content/10.1...
Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching is now available on bioRxiv!
www.biorxiv.org/content/10.1...
Reposted by Chi-Min Ho
This one is a bit of a departure from the usual and definitely a work in progress!
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
September 13, 2025 at 12:05 AM
This one is a bit of a departure from the usual and definitely a work in progress!
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
Reposted by Chi-Min Ho
🚀 NY-CURES Launch — Championing State-Wide Investment in Science! 🚀
Our new coalition advocates for sustained public support of New York’s vibrant biomedical research ecosystem — creating cures, economic gains & strengthening communities.
Sign up. Stay informed. Join the movement: www.nycures.org/
Our new coalition advocates for sustained public support of New York’s vibrant biomedical research ecosystem — creating cures, economic gains & strengthening communities.
Sign up. Stay informed. Join the movement: www.nycures.org/
September 4, 2025 at 4:32 PM
🚀 NY-CURES Launch — Championing State-Wide Investment in Science! 🚀
Our new coalition advocates for sustained public support of New York’s vibrant biomedical research ecosystem — creating cures, economic gains & strengthening communities.
Sign up. Stay informed. Join the movement: www.nycures.org/
Our new coalition advocates for sustained public support of New York’s vibrant biomedical research ecosystem — creating cures, economic gains & strengthening communities.
Sign up. Stay informed. Join the movement: www.nycures.org/
Reposted by Chi-Min Ho
Stopping Ribosomes in Action!: a publication highlight from @keandreyam.bsky.social featuring the lab of @cmholab.bsky.social of @columbiauniversity.bsky.social & colleagues.
Read more here: buff.ly/jWYKq7r
#SBGrid #CryoET
Read more here: buff.ly/jWYKq7r
#SBGrid #CryoET
Stopping Ribosomes in Action!
SBGrid member Chi-Min Ho uses cryo-electron tomography to map malaria’s ribosomes at work.
buff.ly
September 8, 2025 at 4:05 PM
Stopping Ribosomes in Action!: a publication highlight from @keandreyam.bsky.social featuring the lab of @cmholab.bsky.social of @columbiauniversity.bsky.social & colleagues.
Read more here: buff.ly/jWYKq7r
#SBGrid #CryoET
Read more here: buff.ly/jWYKq7r
#SBGrid #CryoET
Reposted by Chi-Min Ho
Want to know how lipidation of some nascent chains takes place by NMT2-- Check our latest work on how NAC couples Protein Synthesis with Nascent Polypeptide Myristoylation on the Ribosome out today @embojournal.org: www.embopress.org/doi/full/10....
August 26, 2025 at 11:00 AM
Want to know how lipidation of some nascent chains takes place by NMT2-- Check our latest work on how NAC couples Protein Synthesis with Nascent Polypeptide Myristoylation on the Ribosome out today @embojournal.org: www.embopress.org/doi/full/10....
Thanks Judy! Hope to see you again at 3DEM GRC next year :)
August 19, 2025 at 8:44 PM
Thanks Judy! Hope to see you again at 3DEM GRC next year :)
Thank you Freddy!! Miss seeing you at BoP, I got used to hanging out every year! Hoping to attend BioMalPar next spring, so hopefully we get a chance to catch up then :)
August 19, 2025 at 8:38 PM
Thank you Freddy!! Miss seeing you at BoP, I got used to hanging out every year! Hoping to attend BioMalPar next spring, so hopefully we get a chance to catch up then :)
Reposted by Chi-Min Ho
Cryo-EM of fertilisation complex Pfs230-Pfs48/45 from malaria parasites! 💕
FREE ACCESS reprint link found here: findaresearcher.wehi.edu.au/tham
📱click on 🌎 and last link
💻 last link on the left hand side
#malaria #transmission #cryoEM #ScienceResearch
www.science.org/doi/10.1126/...
FREE ACCESS reprint link found here: findaresearcher.wehi.edu.au/tham
📱click on 🌎 and last link
💻 last link on the left hand side
#malaria #transmission #cryoEM #ScienceResearch
www.science.org/doi/10.1126/...
Cryo-EM structure of endogenous Plasmodium falciparum Pfs230 and Pfs48/45 fertilization complex
Malaria parasite fertilization occurs in the midgut of a female Anopheles mosquito. Blocking fertilization within the mosquito can prevent malaria transmission. Plasmodium falciparum Pfs230 and Pfs48/...
www.science.org
August 8, 2025 at 10:48 PM
Cryo-EM of fertilisation complex Pfs230-Pfs48/45 from malaria parasites! 💕
FREE ACCESS reprint link found here: findaresearcher.wehi.edu.au/tham
📱click on 🌎 and last link
💻 last link on the left hand side
#malaria #transmission #cryoEM #ScienceResearch
www.science.org/doi/10.1126/...
FREE ACCESS reprint link found here: findaresearcher.wehi.edu.au/tham
📱click on 🌎 and last link
💻 last link on the left hand side
#malaria #transmission #cryoEM #ScienceResearch
www.science.org/doi/10.1126/...