Lightnews — Scholar-powered news

Reposted by Daniel Durocher

CortésLab @corteslab.bsky.social · 6d

Kickstarting our Bluesky🦋 account with great news!
Our REPAIRome study finally out today in
@science.org
www.science.org/doi/10.1126/...

A comprehensive genetic catalog of human double-strand break repair

The analysis of DNA sequence outcomes provides molecular insights into double-strand break (DSB) repair mechanisms. Using parallel in-pool profiling of Cas9-induced insertions and deletions (indels) w...

www.science.org

7 26 71

Reposted by Daniel Durocher

Mcclelland lab @mcclellandlab.bsky.social · 8d

We are looking for our next PhD student to join us 🤩 www.mcclellandlab.com to understand cancer radiation therapy's impact on DNA damage repair and chromosomal instability. Prestigious funding from CRUK RadNet. Please contact me informally - deadline 5th Nov! crukradnet.colcc.ac.uk/phd-students...

8 16

Daniel Durocher @durocher1.bsky.social · 8d

I suppose it is a calibration tool for food chemistry labs (calories, texture, content) but it's wild indeed!

1 1

Daniel Durocher @durocher1.bsky.social · 14d

I really wonder what a $2K set of peanut butter jars from the NATIONAL INSTITUTES OF STANDARDS & TECHNOLOGY could be for?

2 11

Reposted by Daniel Durocher

Mathew Garnett @mgarnett.bsky.social · 20d

The Garnett Lab is excited to launch DepMap Miner, a new tool to explore cancer dependency data!
Built on the Sanger DepMap, the DepMap Miner brings together multi-omic and functional datasets for over 1300 cancer cell models.

Start mining data now: dataminer.depmap.sanger.ac.uk

2 23 55

Reposted by Daniel Durocher

Sylvie Noordermeer @sylvie-noordermeer.bsky.social · 22d

Happy to share our latest research on the BRCA1-BRCTs now online.

academic.oup.com/nar/article/...

Congrats to first author Venda for putting it all together! This project was a great collaboration with the @titiasixma.bsky.social lab who helped us give structural meaning to our observations.

Validate User

academic.oup.com

1 6 19

Daniel Durocher @durocher1.bsky.social · Sep 10

Kaima Tsukuda and @andrewblackford.bsky.social have a highly complementary study that indicates that mutations in DDIAS (and CIP2A) caused neurodevelopmental defects, pointing a role for this pathway in development. /fin

www.biorxiv.org/content/10.1...

DDIAS is a single-stranded DNA-binding effector of the TOPBP1-CIP2A complex in mitosis

DNA double-strand breaks and unresolved DNA replication intermediates are particularly dangerous during mitosis. Paradoxically, cells inactivate canonical DNA repair mechanisms during chromosome segre...

www.biorxiv.org

2

Daniel Durocher @durocher1.bsky.social · Sep 10

Thanks to all authors and collaborators, chiefly @yiboxue.bsky.social but that's not all!

1

Daniel Durocher @durocher1.bsky.social · Sep 10

Why is an ssDNA-binding protein involved in the CIP2A pathway? Our data suggests it may be to prevent ssDNA from accumulating in mitosis.

We propose that mitotic ssDNA is a liability for the integrity of chromosomes during cell division, and that a function of CIP2A-TOPBP1-DDIAS is suppress it.

1 1

Daniel Durocher @durocher1.bsky.social · Sep 10

We were puzzled by the fact that DDIAS has an OB-fold domain which often acts as an ssDNA-binding domain.

We find that DDIAS binds to ssDNA. This activity is not involved in its recruitment to DNA damage sites, but it is necessary for its function in genome maintenance.

1 1

Daniel Durocher @durocher1.bsky.social · Sep 10

Like CIP2A, DDIAS accumulates at DNA lesions specifically during mitosis, and it is recruited to the CIP2A-TOPBP1 complex via a phosho-dependent interaction with the TOPBP1 C-terminus.

DDIAS loss, like that of CIP2A, impairs the viability of BRCA1/2-deficient cells.

1 1

Daniel Durocher @durocher1.bsky.social · Sep 10

New work from the lab! 🔬

We report that DDIAS is a new component of the mitotic CIP2A-TOPBP1 pathway of genome maintenance. 1/n

www.biorxiv.org/content/10.1...

Mitotic single-stranded DNA suppression by DDIAS

Incomplete DNA replication and chromosome breakage during mitosis pose major threats to chromosome segregation. The CIP2A-TOPBP1 complex acts to mitigate this peril, but its exact role is not yet unde...

www.biorxiv.org

2 17 47

Reposted by Daniel Durocher

Andrew Blackford @andrewblackford.bsky.social · Sep 10

Excited to share our lab’s latest preprint! We identify DDIAS as a novel single-stranded DNA-binding component of the TOPBP1–CIP2A complex, which acts in a mitotic DNA damage response pathway to protect chromosome integrity. Short summary below, and read it here: www.biorxiv.org/content/10.1...

DDIAS is a single-stranded DNA-binding effector of the TOPBP1-CIP2A complex in mitosis

DNA double-strand breaks and unresolved DNA replication intermediates are particularly dangerous during mitosis. Paradoxically, cells inactivate canonical DNA repair mechanisms during chromosome segre...

www.biorxiv.org

2 11 36

Reposted by Daniel Durocher

Research at Sinai Health @sinaihealth.bsky.social · Sep 9

Job Alert 🚨

Are you an emerging leader in biomedical discovery or population health data science?
We are looking for outstanding candidates to join our institute!

Learn more & apply:

Biomedical Discovery ➡️ apply.interfolio.com/172703

Population Health Data Science ➡️ apply.interfolio.com/172529

3 4

Daniel Durocher @durocher1.bsky.social · Sep 9

Wishing you many more!

1 1

Reposted by Daniel Durocher

Jim Mansfield @jrmansfield.bsky.social · Aug 23

New from @hartjackson.bsky.social: 104‑plex #IMC on 3 serial PDAC sections, 200+ patients, whole-slide & cellular modes. Spatial proteomics guides LCM + genomics to define tumor–immune–stromal spectra. A tour de force.

biorxiv.org/content/10.1...

@standardbiology.bsky.social #SpatialBio #IMC

Integrated spatial proteomics of human PDAC uncovers an expanded tumour–immune– stroma spectrum with genomic associations

Distinctively, pancreatic ductal adenocarcinoma (PDAC) consists of sparse tumour lesions intertwined with extensive desmoplastic stroma. The complexity of tumour–microenvironment interactions within t...

biorxiv.org

4 6

Daniel Durocher @durocher1.bsky.social · Aug 28

JOB ALERT 🚨 We are hiring TWO principal investigators in cell, molecular, systems, or chemical biology in Toronto, Canada at @sinaihealth.bsky.social. We provide a generous startup, fully funded salary and academic appointment at U of Toronto.

www.nature.com/naturecareer...

Please repost!

170 130

Daniel Durocher @durocher1.bsky.social · Aug 27

LOLSOB

Daniel Durocher @durocher1.bsky.social · Aug 22

Welcome Alissa! We are very excited to have you with us!

alissa greenwald @alissacg.bsky.social · Aug 22

I'm thrilled to share that I'll be starting my lab (www.greenwald-lab.org) at the Lunenfeld-Tanenbaum Research Institute @sinaihealth.bsky.social and joining @moleculargenetics.bsky.social at the University of Toronto as an assistant professor this fall. 1/

1 3

Reposted by Daniel Durocher

Ian Scott @icscott.bsky.social · Aug 21

Teaching stream faculty position in molecular genetics!
jobs.utoronto.ca/job/Toronto-...

Assistant Professor, Teaching Stream (CLTA) - Molecular Biology, Genetics, and Microbiology

jobs.utoronto.ca

4 4

Daniel Durocher @durocher1.bsky.social · Aug 16

Félicitations Amélie!!!

1 1

Reposted by Daniel Durocher

AmélieFT@AFTLab @amelieft.bsky.social · Aug 13

I am very excited to share the newest paper of the lab, a huge amount of work led by our talented PhD student Maxime Galloy, in close collaboration with Andréanne Blondeau, our dedicated research assistant for 10 years! 🙌

www.cell.com/molecular-ce...

Ubiquitination of the histone variant mH2A1.2 prevents toxic RAD18 accumulation at a subset of genomic loci upon replication stress

Using biochemical assays and a mutant disrupting RNF168-dependent ubiquitination of the histone variant macroH2A1.2, Galloy et al. identified an unexpected role for histone mH2A1.2 ubiquitination in p...

www.cell.com

2 13 29

Reposted by Daniel Durocher

Michael Erb @michael-erb.bsky.social · Jul 31

New work out today introducing PCIPs, heterobifunctional chemical inducers of proximity that inhibit DNA repair by recruiting BET proteins to PARP2. Great work uncovering a new form of event-driven pharmacology by Bryce/Eric/Erin and the rest of the team. (1/3)

www.biorxiv.org/content/10.1...

Rewiring DNA repair with PARP-based chemical inducers of proximity

Chemical inducers of proximity (CIPs) can elicit durable, and often neomorphic, biological effects through the formation of a ternary complex, even at low equilibrium occupancy of their targets. This ...

www.biorxiv.org

1 4 18

Reposted by Daniel Durocher

Ian Scott @icscott.bsky.social · Jul 27

Great opportunity to join the vibrant developmental biology in Toronto (and boss around me and @wbderry.bsky.social).

jobs.sciencecareers.org/job/673968/p...

Program Head – Developmental, Stem Cell, and Cancer Biology - Toronto (Region), Ontario (CA) job with The Hospital for Sick Children (SickKids) Research Institute | 673968

Provide leadership and mentorship to members of the Developmental, Stem Cell & Cancer and influence the scientific direction of the Research Institute

jobs.sciencecareers.org

8 9

Reposted by Daniel Durocher

John Rubinstein @johnrubinstein.bsky.social · Jul 25

🇨🇦🚨Job alert🚨🇨🇦
We're looking for a senior scientist (program head) in the developmental, stem cell, and cancer biology program at the Hospital for Sick Children

Amy Wong 🇨🇦 @dramywong.bsky.social · Jul 25

We are hiring 📣 Join our mighty team! See posting below 👇

www.sickkids.ca/en/research/...

www.sickkids.ca

5 5