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Hamish Stewart @hamishs.bsky.social · 12d

Congratulations!

Reposted by Hamish Stewart

kmechtler.bsky.social @kmechtler.bsky.social · Aug 28

It is an honor to inform you that our manuscript with low resolution FAIMS is now online

www.biorxiv.org/content/10.1...

Low-resolution FAIMS for increased peptide coverage in low-load and single-cell proteomics

Field Asymmetric Ion Mobility Spectrometry (FAIMS) enhances signal to noise ratio filtering ions based on their differential mobility, making it an indispensable tool for single-cell and low-input pro...

www.biorxiv.org

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Hamish Stewart @hamishs.bsky.social · Aug 27

👀 A simple optimization giving substantial benefits. A little like widening an over-tight quadrupole window to help transmission.

IonOpticks @ionopticks.bsky.social · Aug 27

A great showcase of Aurora Series columns at Thermo's user group meeting at #ESCP2025: lowering FAIMS resolution on the Orbitrap Astral can increase peptide IDs by up to 25% for HeLa digests and 17% for real H460 cells when coupled to a Vanquish Neo with Aurora Rapid 8x75 columns.
bit.ly/4g1jrUW

Low resolution FAIMS: Up to 25 % more peptide IDs with low resolution FAIMS at OrbiTrap Astral! What’s your opinion about it? Many thanks to Dominic Hoch for the excellent cooperation. TFS user… | ...

Low resolution FAIMS: Up to 25 % more peptide IDs with low resolution FAIMS at OrbiTrap Astral! What’s your opinion about it? Many thanks to Dominic Hoch for the excellent cooperation. TFS user mee...

bit.ly

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Hamish Stewart @hamishs.bsky.social · Aug 22

That's wild.

Hamish Stewart @hamishs.bsky.social · Aug 16

My great colleague Tabi is featured in JASMS. Check out his story.

BioMassSpec @realbiomassspec.bsky.social · Aug 16

Faces of Mass Spectrometry/Tabiwang Arrey #JASMS pubs.acs.org/doi/10.1021/...

Faces of Mass Spectrometry/Tabiwang Arrey

You have not visited any articles yet, Please visit some articles to see contents here.

pubs.acs.org

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Hamish Stewart @hamishs.bsky.social · Aug 15

Is a lager shandy on the list.

Hamish Stewart @hamishs.bsky.social · Aug 11

The biggest use case for MS3 has been TMT, so presumably the Gygi groups papers on the Ascend, Eclipse and previous instruments are most relevant.

pubs.acs.org/doi/10.1021/...

Deep Proteomic Compound Profiling with the Orbitrap Ascend Tribrid Mass Spectrometer Using Tandem Mass Tags and Real-Time Search

Tandem mass tags (TMT) and tribrid mass spectrometers are a powerful combination for high-throughput proteomics with high quantitative accuracy. Increasingly, this technology is being used to map the ...

pubs.acs.org

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Hamish Stewart @hamishs.bsky.social · Aug 11

Depends on timescale and applications I guess. MS3 is currently limited to the tribrid line, where there have been big improvements recently with parallel ion accumulation for MS3, and the new Stellar quad-ion trap where it can run 40Hz MS3.

assets.thermofisher.cn/TFS-Assets/C...

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Reposted by Hamish Stewart

Bavarian Center for Biomolecular Mass Spectrometry @baybioms.bsky.social · Jul 22

🎉 BayBioMS turns 10! 🎉

2025 marks our 10th anniversary of Mapping Molecular Mountains.

Thus, we’re excited to invite you to the 10 Years of #BayBioMS #Symposium – a celebration of a decade of accelerating #MassSpectrometry research at the @tum.de!

🧵(1/6)

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Hamish Stewart @hamishs.bsky.social · Jul 22

We had to rewrite AGC control completely and lost some of the legacy functionality.

The eDR/multiplexed SIM implementation, while ultimately relatively simple, took quite a lot of effort to implement.

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Hamish Stewart @hamishs.bsky.social · Jul 21

One learns something every day

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Hamish Stewart @hamishs.bsky.social · Jul 21

That's interesting, I would've assumed all Exploris instruments (aside MX) would have the same rules for multiplexed SIM.

Actually surprising older "helpful" restrictions didn't stick across generations.

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Hamish Stewart @hamishs.bsky.social · Jul 20

Ah! This one's definitely aimed at Orbitrap.

For Astral analyser itself there's some debate whether multiplexing will be useful. Maybe multiple scans with different mass ranges, but usually scan averaging alone should be preferable.

Perhaps ideally the combination of Astral and OT full MS scans...

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Hamish Stewart @hamishs.bsky.social · Jul 20

I'm sorry, I don't follow. Which patent do you mean here?

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Hamish Stewart @hamishs.bsky.social · Jul 20

This paper would have been largely complete by the time the MAP-MS preprint came out, barely two months ago. A very easy oversight.

I think I'm the only one with input into both projects, and this didn't even occur to me on my reviewed.

The common root of the Boxcar method is cited however.

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Reposted by Hamish Stewart

JesperOlsenLab @jesperolsenlab.bsky.social · Jul 19

🚀 Check out our preprint on the new Thermo Scientific Orbitrap Astral Zoom MS 💫👇🏼

www.biorxiv.org/content/10.1...

Higher-Throughput Proteome Profiling Enabled by Parallelized Pre-Accumulation and Optimized Ion Processing in the Orbitrap Astral Zoom Mass Spectrometer

High-throughput proteomics is critical for understanding biological processes, enabling large-scale studies such as biomarker discovery and systems biology. However, current mass spectrometry technolo...

www.biorxiv.org

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Reposted by Hamish Stewart

JesperOlsenLab @jesperolsenlab.bsky.social · Jul 1

Boosting Orbitrap MS/MS speed to ∼70 Hz? 🚀

Read how we did it in our latest paper 👇

pubs.acs.org/doi/10.1021/...

Enhancing Tandem MS Sensitivity and Peptide Identification via Ion Preaccumulation in an Orbitrap Mass Spectrometer

High-throughput mass spectrometry-based proteomics has gained increasing interest for both academic and industrial applications. As implementation of faster gradients has facilitated higher sample thr...

pubs.acs.org

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Hamish Stewart @hamishs.bsky.social · Jun 29

It was simply that with high enough resolution mobility separation then one may gain sufficient selectivity in DIA without quadrupole isolation.

It's not a new concept, but mobility separators in the past weren't suitable (though there's a pasef derivative with very wide quad windows)

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Reposted by Hamish Stewart

Royal Society of Chemistry @rsc.org · Jun 25

🎉 Today we reveal the 2025 RSC Prize winners! 🎉

Celebrating excellence across research, diversity, tech roles, apprentices and partnerships. Join us in congratulating the remarkable individuals and teams advancing chemistry for society. buff.ly/K2un4O1 #Chemistry #ChemSky

Congratulations banner from the Royal Society of Chemistry featuring a medallion with symbolic icons representing various scientific elements and achievements, surrounded by a stardust background with the text "#RSCPrizes".

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Reposted by Hamish Stewart

Chris Ashwood @cashwood.proteaglyco.com · Jun 25

Orbitrap Astral Team receive the RSC 2025 Analytical Science Horizon Prize: Robert Boyle Prize.

Interesting information about what each role does at TFS.

A good read:
www.rsc.org/standards-an...

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Hamish Stewart @hamishs.bsky.social · Jun 13

A great loss.

Reposted by Hamish Stewart

Mike Goodwin @massspecpro.bsky.social · Jun 12

RIP Alan Marshall, one of the greatest mass spectrometrists ever, and a great human to boot.

nationalmaglab.org/careers/meet...

Alan Marshall: A scientist and a Gentleman - MagLab

Meet one of the greatest innovators in the history of mass spectrometry, hard at work.

nationalmaglab.org

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Hamish Stewart @hamishs.bsky.social · Jun 5

Very keen on the promise of showing what different instrument classes really mean by their intensity classification.

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Hamish Stewart @hamishs.bsky.social · Jun 3

Cheers! 😀

Hamish Stewart @hamishs.bsky.social · Jun 2

I feel this is somewhat unfair. It's well understood that Orbitrap resolution is transient length dependent.

What allows shorter transients on the Excedion Pro MS is that we've implemented a parallel pre-accumulation process that prevents the otherwise unacceptable loss of duty cycle at such speed.

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