Chris Ashwood
@cashwood.proteaglyco.com
Director of Protea Glycosciences
Accelerating glycoscience research
Accelerating glycoscience research
Pinned
Chris Ashwood
@cashwood.proteaglyco.com
· Nov 21
Given the influx of people at bsky, thought I would formally introduce myself 👋
I'm Chris, an Australian glycoscientist who has studied the field for >10 years now (across both AU and US), starting (MRes and PhD) in the lab of Prof. Nicki Packer.
I believe LC-MS glyco still has a lot to discover.
I'm Chris, an Australian glycoscientist who has studied the field for >10 years now (across both AU and US), starting (MRes and PhD) in the lab of Prof. Nicki Packer.
I believe LC-MS glyco still has a lot to discover.
Excited to share our (@maiakelly.bsky.social and @proteaglyco.bsky.social) most recent research, showing how negative ion supercharging achieves high quality oxidatively released glycan characterisation
TLDR: Singly charge MS2 bad, multiply charge MS2 good.
www.biorxiv.org/content/10.1...
#glycotime
TLDR: Singly charge MS2 bad, multiply charge MS2 good.
www.biorxiv.org/content/10.1...
#glycotime
Anion supercharging enables structural assignment of oxidatively released O-glycans
O-glycan analysis faces analytical challenges due to poor fragmentation characteristics of oxidatively released O-glycan acids, which can preserve labile O-acetyl modifications but suffer from charge ...
www.biorxiv.org
November 26, 2025 at 5:57 AM
Excited to share our (@maiakelly.bsky.social and @proteaglyco.bsky.social) most recent research, showing how negative ion supercharging achieves high quality oxidatively released glycan characterisation
TLDR: Singly charge MS2 bad, multiply charge MS2 good.
www.biorxiv.org/content/10.1...
#glycotime
TLDR: Singly charge MS2 bad, multiply charge MS2 good.
www.biorxiv.org/content/10.1...
#glycotime
Reposted by Chris Ashwood
ARC are considering a restructure in which jobs there will be lost, a source tells me.
This seems ridiculous when they're about to completely overhaul the entire grants system – they've never undertaken anything so large before!
I've long said ARC needs MANY MORE staff, & should do more, not less.
This seems ridiculous when they're about to completely overhaul the entire grants system – they've never undertaken anything so large before!
I've long said ARC needs MANY MORE staff, & should do more, not less.
November 25, 2025 at 10:26 PM
ARC are considering a restructure in which jobs there will be lost, a source tells me.
This seems ridiculous when they're about to completely overhaul the entire grants system – they've never undertaken anything so large before!
I've long said ARC needs MANY MORE staff, & should do more, not less.
This seems ridiculous when they're about to completely overhaul the entire grants system – they've never undertaken anything so large before!
I've long said ARC needs MANY MORE staff, & should do more, not less.
Reposted by Chris Ashwood
My former student 闫石 Yan Shi is now Privatdozent - he successfully held his Habilitation lecture today!
#ProudofAlumni
#ProudofAlumni
November 25, 2025 at 4:34 PM
My former student 闫石 Yan Shi is now Privatdozent - he successfully held his Habilitation lecture today!
#ProudofAlumni
#ProudofAlumni
Cannot hide my joy, relief, excitement being able to share the outcome of persevering (to say the least) collaborative work. So... the MS glyco(proteomics) guidelines are published!! www.mcponline.org/article/S153...
Many thanks to @beilstein-institut.bsky.social @glycosmos.bsky.social @sib.swiss
Many thanks to @beilstein-institut.bsky.social @glycosmos.bsky.social @sib.swiss
Update and new implementation of the MIRAGE reporting guidelines for mass spectrometry experiments in glycoscience
The MIRAGE (Minimum Information Required for A Glycomics Experiment) guidelines for
mass spectrometry (MS) data were initially developed to standardize the reporting
of instrumentation, data acquisiti...
www.mcponline.org
November 25, 2025 at 9:41 AM
Two #teammassspec MS grants for Australia!
🚨 #LIEF #LE26 announcement:
❗️Outcomes announced publicly for Linkage Infrastructure, Equipment and Facilities 2026❗️
See ARC's RMS for list ➡️ https://rms.arc.gov.au/RMS/Report/Download/Report/a3f6be6e-33f7-4fb5-98a6-7526aaa184cf/287
/bot
❗️Outcomes announced publicly for Linkage Infrastructure, Equipment and Facilities 2026❗️
See ARC's RMS for list ➡️ https://rms.arc.gov.au/RMS/Report/Download/Report/a3f6be6e-33f7-4fb5-98a6-7526aaa184cf/287
/bot
November 25, 2025 at 3:27 AM
Two #teammassspec MS grants for Australia!
Three #glycotime grants!
🚨 #DECRA #DE26 announcement:
❗️Outcomes announced publicly for Discovery Early Career Researcher Award 2026❗️
See ARC's RMS for list ➡️ https://rms.arc.gov.au/RMS/Report/Download/Report/a3f6be6e-33f7-4fb5-98a6-7526aaa184cf/285
/bot
❗️Outcomes announced publicly for Discovery Early Career Researcher Award 2026❗️
See ARC's RMS for list ➡️ https://rms.arc.gov.au/RMS/Report/Download/Report/a3f6be6e-33f7-4fb5-98a6-7526aaa184cf/285
/bot
November 25, 2025 at 12:39 AM
Three #glycotime grants!
Reposted by Chris Ashwood
Hello #glycotime! GlycoShape makes its debut on bsky today 🦋🎉 Follow us for news, updates and fun-facts (I take full responsibility for those, Ojas @ojas-singh.bsky.social 😆) below ⬇️
Hello bsky 🦋 GlycoShape is now here to give you updates on our glycan 3D database, toolbox, glyco fun-facts and news!
We just updated server, so clear your browser's cache, and build some glycoproteins with us 🍬
We'll have great new tools coming up soon, so follow us for some exciting #glycotime
We just updated server, so clear your browser's cache, and build some glycoproteins with us 🍬
We'll have great new tools coming up soon, so follow us for some exciting #glycotime
a close up of a feather on a rock with the words `` hello '' written in white letters .
ALT: a close up of a feather on a rock with the words `` hello '' written in white letters .
media.tenor.com
November 24, 2025 at 4:53 PM
Hello #glycotime! GlycoShape makes its debut on bsky today 🦋🎉 Follow us for news, updates and fun-facts (I take full responsibility for those, Ojas @ojas-singh.bsky.social 😆) below ⬇️
Reposted by Chris Ashwood
Meeting Report on the 1st GlyCosmos Symposium & Jamboree —Community-Driven Expansion of Glycan-Binding Molecules Resource#glycotimem#Glycobiologygy
Meeting Report on the 1st GlyCosmos Symposium & Jamboree —Community-Driven Expansion of Glycan-Binding Molecules Resources
Chiaki Nagai-Okatani, Masae Hosoda, Winnie Hsiung, Takashi Sato, Hiroaki Sakaue, Sayaka Fuseya, Eriko Fukuda, Issaku Yamada, Kiyoko F Aoki-Kinoshita, Atsus
academic.oup.com
November 23, 2025 at 1:39 PM
Meeting Report on the 1st GlyCosmos Symposium & Jamboree —Community-Driven Expansion of Glycan-Binding Molecules Resource#glycotimem#Glycobiologygy
Reposted by Chris Ashwood
Interesting paper by a team around Takahiro Takayama in Analytical Chemistry. A systematic study of the effect of deuteration on retention time and an optimization of stationary and liquid phases to reduce the effect for better quantitative analysis. doi.org/10.1021/acs....
#Chromatography #LCMS
#Chromatography #LCMS
Mechanistic Study of the Deuterium Effect in Chromatographic Separation for Chemical-Tagging Metabolomics and Its Application to Biomarker Discovery in Metabolic Dysfunction-Associated Steatohepatitis
Over the past decade, numerous metabolomics techniques have been developed using liquid chromatography–mass spectrometry (LC-MS). These methodologies have yielded significant findings and facilitated ...
doi.org
November 23, 2025 at 4:06 PM
Interesting paper by a team around Takahiro Takayama in Analytical Chemistry. A systematic study of the effect of deuteration on retention time and an optimization of stationary and liquid phases to reduce the effect for better quantitative analysis. doi.org/10.1021/acs....
#Chromatography #LCMS
#Chromatography #LCMS
Reposted by Chris Ashwood
In fermented foods microbes convert some of the food substrate into microbial protein. We found that in some fermented foods up to 10% of the total protein is microbial and that some of these microbial proteins have functions that might explain some of the beneficial effects of fermented foods.
Assessing the diversity and functional profile of the “microbial proteome” in fermented foods
Fermented foods are staples in diets worldwide and are known for their health benefits. Microorganisms are the key to fermented food production as they convert raw substrates into digestible, nutritio...
www.biorxiv.org
November 23, 2025 at 8:09 PM
In fermented foods microbes convert some of the food substrate into microbial protein. We found that in some fermented foods up to 10% of the total protein is microbial and that some of these microbial proteins have functions that might explain some of the beneficial effects of fermented foods.
In case you missed it, Thermo announced a new TSQ - the TSQ Certis. A few minor improvements:
- 900 SRM/s
- Compatible with the hotter HESI ion source
Specification sheets absent, so unsure if it is an improved Altis Plus, or another TSQ.
www.thermofisher.com/au/en/home/i...
- 900 SRM/s
- Compatible with the hotter HESI ion source
Specification sheets absent, so unsure if it is an improved Altis Plus, or another TSQ.
www.thermofisher.com/au/en/home/i...
www.thermofisher.com
November 20, 2025 at 10:10 PM
In case you missed it, Thermo announced a new TSQ - the TSQ Certis. A few minor improvements:
- 900 SRM/s
- Compatible with the hotter HESI ion source
Specification sheets absent, so unsure if it is an improved Altis Plus, or another TSQ.
www.thermofisher.com/au/en/home/i...
- 900 SRM/s
- Compatible with the hotter HESI ion source
Specification sheets absent, so unsure if it is an improved Altis Plus, or another TSQ.
www.thermofisher.com/au/en/home/i...
Reposted by Chris Ashwood
Absolutely, and membership is still cheap. People could easily make an argument for their employer paying their dues and saving money. Back when I was a contractor I got a society membership benefit, so I paid ASMS dues myself (and had my employer pay the much-more-expensive ACS dues)
November 20, 2025 at 8:40 PM
Absolutely, and membership is still cheap. People could easily make an argument for their employer paying their dues and saving money. Back when I was a contractor I got a society membership benefit, so I paid ASMS dues myself (and had my employer pay the much-more-expensive ACS dues)
Reposted by Chris Ashwood
Delighted to share our new pre-print from PhD student Ziyun Zhang joint with @tracyh9494.bsky.social demonstrating that lung alveolar macrophages have a dense glycocalyx and interstitial macrophages do not, @mcrcellmatrix.bsky.social @lydiabeckeriii.bsky.social www.biorxiv.org/content/10.1...
Airway macrophage specific expression of a dense glycocalyx and its remodelling following viral infection
The glycocalyx is best studied for its regulation of immune cell trafficking from the vasculature into inflamed tissues. The glycocalyx is composed of a range of glycans and glyco-proteins that form a...
www.biorxiv.org
November 20, 2025 at 8:13 PM
Delighted to share our new pre-print from PhD student Ziyun Zhang joint with @tracyh9494.bsky.social demonstrating that lung alveolar macrophages have a dense glycocalyx and interstitial macrophages do not, @mcrcellmatrix.bsky.social @lydiabeckeriii.bsky.social www.biorxiv.org/content/10.1...
This is pretty big, the membership definitely pays for itself now
A major new @asms.org member benefit is now live - expanded access to all the Wiley MS journals! ASMS members can now see RCMS, JMS, and MS Reviews issues one year old on a rolling basis. (Members also get new issues of JASMS and IJMS.) Your dues at work! Access through asms.org/publications...
November 20, 2025 at 8:12 PM
This is pretty big, the membership definitely pays for itself now
Reposted by Chris Ashwood
Quick heads up - there will soon be a job posting for a Staff Scientist / Senior Staff Scientist in our lab in Copenhagen to support all our mass spec proteomics adventures!
Great colleagues, lots of cool projects… and you’ll get to work with me 😅
Please DM if you’re interested!
Great colleagues, lots of cool projects… and you’ll get to work with me 😅
Please DM if you’re interested!
November 20, 2025 at 5:09 PM
Quick heads up - there will soon be a job posting for a Staff Scientist / Senior Staff Scientist in our lab in Copenhagen to support all our mass spec proteomics adventures!
Great colleagues, lots of cool projects… and you’ll get to work with me 😅
Please DM if you’re interested!
Great colleagues, lots of cool projects… and you’ll get to work with me 😅
Please DM if you’re interested!
Tried Hypercarb PGC stationary phase but have been looking for another vendor's take on it? Millipore Sigma have just released their Carbon LC capillary flow columns recently:
Available in 1 mm and 0.3 mm ID formats.
www.sigmaaldrich.com/AU/en/substa...
Available in 1 mm and 0.3 mm ID formats.
www.sigmaaldrich.com/AU/en/substa...
www.sigmaaldrich.com
November 20, 2025 at 1:17 PM
Tried Hypercarb PGC stationary phase but have been looking for another vendor's take on it? Millipore Sigma have just released their Carbon LC capillary flow columns recently:
Available in 1 mm and 0.3 mm ID formats.
www.sigmaaldrich.com/AU/en/substa...
Available in 1 mm and 0.3 mm ID formats.
www.sigmaaldrich.com/AU/en/substa...
Reposted by Chris Ashwood
Akkermansia muciniphila has potential as a next-generation #probiotic, but can it claim a niche and persist in a pre-existing, complex microbial community (in vitro)? 🦠🔍
#microsky
#microbiomesky
#glycotime
link.springer.com/article/10.1...
#microsky
#microbiomesky
#glycotime
link.springer.com/article/10.1...
Probiotic Engraftment of Akkermansia muciniphila in an In Vitro Synthetic Microbial Community - Microbial Ecology
Akkermansia muciniphila is a specialist mucin glycan-degrader that is common in the human gut. A. muciniphila is associated with host health and therefore proposed as a next-generation probiotic. Howe...
link.springer.com
November 20, 2025 at 11:27 AM
Akkermansia muciniphila has potential as a next-generation #probiotic, but can it claim a niche and persist in a pre-existing, complex microbial community (in vitro)? 🦠🔍
#microsky
#microbiomesky
#glycotime
link.springer.com/article/10.1...
#microsky
#microbiomesky
#glycotime
link.springer.com/article/10.1...
An alternative to TMTpro:
"Comparison of the single-cell proteomics revealed that the relative quantification made by IBT-16plex was comparable with that produced from TMTpro-16plex."
"Comparison of the single-cell proteomics revealed that the relative quantification made by IBT-16plex was comparable with that produced from TMTpro-16plex."
IBT-16plex-Based Quantitative Proteomics at the Single-Cell Level: Enabling Protein Profiles toward the Single Cells from Mouse Spleen pubs.acs.org/doi/10....
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#proteomics #prot-paper
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#proteomics #prot-paper
November 20, 2025 at 11:46 AM
An alternative to TMTpro:
"Comparison of the single-cell proteomics revealed that the relative quantification made by IBT-16plex was comparable with that produced from TMTpro-16plex."
"Comparison of the single-cell proteomics revealed that the relative quantification made by IBT-16plex was comparable with that produced from TMTpro-16plex."
Reposted by Chris Ashwood
We're so excited to share our published new paper on MUC17-targeting CAR-T cells for gastric cancer!
MUC17 is a safe, tumor-specific mucin. Our MUC17 CAR-T cells show strong antigen-specific killing and in vivo tumor control. Thank you, Marcela, for the support!
jitc.bmj.com/content/13/1...
MUC17 is a safe, tumor-specific mucin. Our MUC17 CAR-T cells show strong antigen-specific killing and in vivo tumor control. Thank you, Marcela, for the support!
jitc.bmj.com/content/13/1...
CAR-T cell therapy targeting MUC17 in gastric tumors
Background Chimeric antigen receptor (CAR)-T cell therapy has achieved significant success in hematologic malignancies; however, its efficacy in solid tumors remains limited. A major limitation is the...
jitc.bmj.com
November 20, 2025 at 3:53 AM
We're so excited to share our published new paper on MUC17-targeting CAR-T cells for gastric cancer!
MUC17 is a safe, tumor-specific mucin. Our MUC17 CAR-T cells show strong antigen-specific killing and in vivo tumor control. Thank you, Marcela, for the support!
jitc.bmj.com/content/13/1...
MUC17 is a safe, tumor-specific mucin. Our MUC17 CAR-T cells show strong antigen-specific killing and in vivo tumor control. Thank you, Marcela, for the support!
jitc.bmj.com/content/13/1...
Reposted by Chris Ashwood
New preprint! We measured temperature- and pH-induced aggregation for over 18,000 natural and de novo designed protein domains!
November 19, 2025 at 9:16 PM
New preprint! We measured temperature- and pH-induced aggregation for over 18,000 natural and de novo designed protein domains!
Exciting new paper from Sciex:
“We have developed an electron-activated dissociation (EAD) device with product isolation functionality for multistage tandem mass spectrometry (MSn)”
Hopefully commercially available soon, the ability to do MSn is invaluable in characterisation workflows.
“We have developed an electron-activated dissociation (EAD) device with product isolation functionality for multistage tandem mass spectrometry (MSn)”
Hopefully commercially available soon, the ability to do MSn is invaluable in characterisation workflows.
Multistage Tandem Mass Spectrometry Using an Electron-Activated Dissociation Device with a Linear Ion Trap and Structural Identification of Cardiolipins #AC pubs.acs.org/doi/10.1021/...
pubs.acs.org
November 19, 2025 at 8:55 PM
Exciting new paper from Sciex:
“We have developed an electron-activated dissociation (EAD) device with product isolation functionality for multistage tandem mass spectrometry (MSn)”
Hopefully commercially available soon, the ability to do MSn is invaluable in characterisation workflows.
“We have developed an electron-activated dissociation (EAD) device with product isolation functionality for multistage tandem mass spectrometry (MSn)”
Hopefully commercially available soon, the ability to do MSn is invaluable in characterisation workflows.
Reposted by Chris Ashwood
Happy to share our lab’s latest paper in Analytical Chemistry! pubmed.ncbi.nlm.nih.gov/41084887/
Our first foray into top-down proteomics led by Dr. Mozhgan Boroumand sought to identify proteoforms of the Senescent Secretome (SASP)-a stepping stone toward human proteoform biomarker candidates. 1/n
Our first foray into top-down proteomics led by Dr. Mozhgan Boroumand sought to identify proteoforms of the Senescent Secretome (SASP)-a stepping stone toward human proteoform biomarker candidates. 1/n
Characterization of the Intact Proteomic Profile of Senescent-Associated Secretory Phenotype by Top-Down Mass Spectrometry - PubMed
Cellular senescence is a stable state of cell-cycle arrest characterized by extensive remodeling of the secretome, known as the senescence-associated secretory phenotype (SASP). The SASP profoundly influences tissue microenvironments and contributes to chronic inflammation and age-related diseases. …
pubmed.ncbi.nlm.nih.gov
November 19, 2025 at 11:20 AM
Happy to share our lab’s latest paper in Analytical Chemistry! pubmed.ncbi.nlm.nih.gov/41084887/
Our first foray into top-down proteomics led by Dr. Mozhgan Boroumand sought to identify proteoforms of the Senescent Secretome (SASP)-a stepping stone toward human proteoform biomarker candidates. 1/n
Our first foray into top-down proteomics led by Dr. Mozhgan Boroumand sought to identify proteoforms of the Senescent Secretome (SASP)-a stepping stone toward human proteoform biomarker candidates. 1/n
Reposted by Chris Ashwood
Reposted by Chris Ashwood
We wrapped up #THEProteomicsShow Alt Proteomics season with Tim Cleland @paleoproteomicist.bsky.social talking paleoproteomics with me and Ben @proteomicsnews.bsky.social. Won't lie, this season was a blast talking about less typical "proteomics". Find it wherever you find fine podcasts or here.
Ep 94 - Alt Proteomics - Dr. Timothy Cleland by The Proteomics Show
For the final episode of the US HUPO sponsored "Alt Proteomics" series highlighting alternative proteomics things, Ben and Ben sit down to talk with Dr. Timothy Cleland, Smithsonian Institution.Keywor...
creators.spotify.com
November 18, 2025 at 7:13 PM
We wrapped up #THEProteomicsShow Alt Proteomics season with Tim Cleland @paleoproteomicist.bsky.social talking paleoproteomics with me and Ben @proteomicsnews.bsky.social. Won't lie, this season was a blast talking about less typical "proteomics". Find it wherever you find fine podcasts or here.
An insightful patent, just from the drawings alone:
ELECTRO SPRAY IONIZATION WITH CONDUCTIVE PEEK VOLTAGE APPLICATION
patentscope.wipo.int/search/en/de...
- Features an exchangeable emitter for prolonged column lifetime
- Replacing the metal aspect of the emitter with carbon makes it biocompatible
ELECTRO SPRAY IONIZATION WITH CONDUCTIVE PEEK VOLTAGE APPLICATION
patentscope.wipo.int/search/en/de...
- Features an exchangeable emitter for prolonged column lifetime
- Replacing the metal aspect of the emitter with carbon makes it biocompatible
November 18, 2025 at 12:33 PM
An insightful patent, just from the drawings alone:
ELECTRO SPRAY IONIZATION WITH CONDUCTIVE PEEK VOLTAGE APPLICATION
patentscope.wipo.int/search/en/de...
- Features an exchangeable emitter for prolonged column lifetime
- Replacing the metal aspect of the emitter with carbon makes it biocompatible
ELECTRO SPRAY IONIZATION WITH CONDUCTIVE PEEK VOLTAGE APPLICATION
patentscope.wipo.int/search/en/de...
- Features an exchangeable emitter for prolonged column lifetime
- Replacing the metal aspect of the emitter with carbon makes it biocompatible