🚨🧠🔬 A major breakthrough in molecular neuroscience:

I am excited to share a new story from our lab, published in accelerated format today by @nature.com:

"Delta-type glutamate receptors are ligand-gated ion channels"

Read more here (free article link): rdcu.be/eGIKz

Happy to share the molecular mechanism of activation of kainate-subtype ionotropic glutamate receptor GluK2 in complex with auxiliary subunits Neto2. In both closed/apo and open states, ligand-binding domain (LBD) layer has 2-fold rotational symmetry!
www.nature.com/articles/s41...

Happy to share a new preprint from the Clarke & Vallese (@fravallese.bsky.social) labs reporting #cryoem structures of two RBC stomatin complexes – with AQP1 & UT-B - continuing the SPFH theme from our recent vault preprint! This was a fun one and has been cooking for a while - read on for more! ⬇️

CryoDRGN-AI: a deep learning-based ab initio reconstruction method for dynamic biomolecular complexes from cryo-ET and cryo-EM data.

www.nature.com/articles/s41...

1/x New story - the structure of the Vault cap, at 2.3Å! Matching a beautiful preprint from @sjorsscheres.bsky.social, we show that the cap of the vault particle has C13 symmetry, in contrast to the 39-fold symmetry of the body. Study lead by talented postdoc @huanli00.bsky.social from my group! ⬇️

Don't say you know your transporter unless you've seen it in a dozen environments!🧪😍

Beautiful & important paper by @leahoffmann.bsky.social @anikabaier.bsky.social @dmitryshvarev.bsky.social @jhschaef.bsky.social @djanuliene.bsky.social @arnemoeller.bsky.social 🥳

www.cell.com/structure/fu...

MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony https://www.biorxiv.org/content/10.1101/2025.01.10.632437v1

Thermo‐TRP #ionchannels regulation by endogenous factors and its physiological function at core body temperature - Kashio - 2025 - Physiological Reports - Wiley Online Library https://buff.ly/3PumuZh

Calmodulin binding is required for calcium mediated TRPA1 desensitization https://www.biorxiv.org/content/10.1101/2024.12.11.627969v1

jobrxiv.org/job/cold-spr.... The HF_Lab has a postdoc position to study the structural and functional aspects of signaling complexes involved in neuroplasticity, cancer immunology, and autoimmunity. Candidates with experience in cryo-EM and/or electrophysiology are highly encouraged to apply.

Interestingly, the vanilloid, melatonin, and mucolipin subfamilies of TRP channels follow a similar pattern of conformational changes upon opening, but inactivation occurs differently across subfamilies. (5/5)

To date, several structures of TRP channels in complexes with interacting proteins were published: TRPV5/6 and TRPC4 with calmodulin, TRPV4 with RhoA, TRPM3 with Gβγ, and TRPC5 with Gαi3. (4/5)

In the apo state, in high-resolution structures, many of these ligand-binding sites are occupied by annular lipids. However, not all lipid sites were reported to be occupied by ligands, so these new sites might be interesting to explore pharmacologically. (3/5)

We compared all structures of TRP channels released on the RCSB by 2024-08-14 and identified 40 distinct ligand-binding sites. (2/5)

Happy to share our new review on the structural pharmacology of TRP channels!
Check out the supplementary table, which includes a list of all ligands, corresponding PDBs of complexes with TRP channels, and reported affinity values.

www.sciencedirect.com/science/arti... #ionchannel #TRPchannel (1/5)

A fun journey of full-length EGFR with Dan Leahy and
@edwardmarcotte.bsky.social at UT Austin @utaustin.bsky.social. See below:
Structure and organization of full-length Epidermal Growth Factor Receptor in extracellular vesicles by cryo-electron tomography www.biorxiv.org/content/10.1...