TJ Krzystek
@lakeerieneuro.bsky.social
PhD in #CellBiology || Senior Scientist 🔬
#Microscopy #Neuroscience #DiseaseModeling #hiPSCs
#Phenomics #TargetBiology #DrugDiscovery #LiveImaging
#BillsMafia || Posts represent only myself
#Microscopy #Neuroscience #DiseaseModeling #hiPSCs
#Phenomics #TargetBiology #DrugDiscovery #LiveImaging
#BillsMafia || Posts represent only myself
Pinned
TJ Krzystek
@lakeerieneuro.bsky.social
· Oct 19
I feel the science-to-nonsense ratio is much better on this site:
Enjoy these jittery mitochondria sifting through axons of patient-derived motor neurons thanks to the pkMito probe from @spirochrome.com
#neuroscience #science #microscopy #bioimaging #mitochondria
Enjoy these jittery mitochondria sifting through axons of patient-derived motor neurons thanks to the pkMito probe from @spirochrome.com
#neuroscience #science #microscopy #bioimaging #mitochondria
Reposted by TJ Krzystek
We just released a new major version of TrackMate (v8), the cell and organelle tracking plugin of Fiji.
It ships many new features, detailed below, but that are articulated around the following:
It ships many new features, detailed below, but that are articulated around the following:
November 20, 2025 at 11:42 AM
We just released a new major version of TrackMate (v8), the cell and organelle tracking plugin of Fiji.
It ships many new features, detailed below, but that are articulated around the following:
It ships many new features, detailed below, but that are articulated around the following:
Pleased to share my latest review article with @tandfresearch.bsky.social, taking a deep dive into neuronal autophagy, how Huntingtin coordinates it, and how the process goes awry in Huntington’s disease.
doi.org/10.1080/27694127.2025.2472450
#Neuroscience #Autophagy #HuntingtonsDisease
doi.org/10.1080/27694127.2025.2472450
#Neuroscience #Autophagy #HuntingtonsDisease
June 3, 2025 at 10:27 PM
Pleased to share my latest review article with @tandfresearch.bsky.social, taking a deep dive into neuronal autophagy, how Huntingtin coordinates it, and how the process goes awry in Huntington’s disease.
doi.org/10.1080/27694127.2025.2472450
#Neuroscience #Autophagy #HuntingtonsDisease
doi.org/10.1080/27694127.2025.2472450
#Neuroscience #Autophagy #HuntingtonsDisease
Always exciting when a dissertation chapter makes it to publication.
Thrilled to share this work in Cell Death & Disease, exploring putative roles of GSK3β and ERK1 phosphorylation in Huntington’s disease.
Full article: doi.org/10.1038/s414...
Huge thanks to all co-authors & contributors! 🦾
Thrilled to share this work in Cell Death & Disease, exploring putative roles of GSK3β and ERK1 phosphorylation in Huntington’s disease.
Full article: doi.org/10.1038/s414...
Huge thanks to all co-authors & contributors! 🦾
Opposing roles for GSK3β and ERK1-dependent phosphorylation of huntingtin during neuronal dysfunction and cell death in Huntington’s disease - Cell Death & Disease
Cell Death & Disease - Opposing roles for GSK3β and ERK1-dependent phosphorylation of huntingtin during neuronal dysfunction and cell death in Huntington’s disease
doi.org
April 23, 2025 at 10:11 PM
Always exciting when a dissertation chapter makes it to publication.
Thrilled to share this work in Cell Death & Disease, exploring putative roles of GSK3β and ERK1 phosphorylation in Huntington’s disease.
Full article: doi.org/10.1038/s414...
Huge thanks to all co-authors & contributors! 🦾
Thrilled to share this work in Cell Death & Disease, exploring putative roles of GSK3β and ERK1 phosphorylation in Huntington’s disease.
Full article: doi.org/10.1038/s414...
Huge thanks to all co-authors & contributors! 🦾
Reposted by TJ Krzystek
Do you want to hear the latest and greatest about the neuronal cytoskeleton? Join us in Chile next April for #NeuroCyto2025, organized by @monicamsousa.bsky.social, Kassandra Ori-McKenney and Tomás Falzone! Registration is open until March 5: neurocytoskeleton.com/2025/
January 12, 2025 at 2:57 PM
Do you want to hear the latest and greatest about the neuronal cytoskeleton? Join us in Chile next April for #NeuroCyto2025, organized by @monicamsousa.bsky.social, Kassandra Ori-McKenney and Tomás Falzone! Registration is open until March 5: neurocytoskeleton.com/2025/
Exciting work! 🎉
Nucleoids are known to be regularly spaced along mitochondria, important for their inheritance and maintenance of the local proteome. We identified pearling, a Plateau-Rayleigh type instability described since the 1980s, to be responsible: t.ly/KKmGK. Collab with JLS lab @hhmijanelia.bsky.social
December 24, 2024 at 1:42 PM
Exciting work! 🎉
Likely my final #FluorescenceFriday post of 2024! 🔬
Here’s a kinetic showcasing pkMito dynamics and the heterogeneity of axonal mitochondria
I'm looking forward to all of the incredible microscopy from this community in 2025.
Happy Holidays everyone! ☃️❄️
Here’s a kinetic showcasing pkMito dynamics and the heterogeneity of axonal mitochondria
I'm looking forward to all of the incredible microscopy from this community in 2025.
Happy Holidays everyone! ☃️❄️
December 20, 2024 at 6:02 PM
Likely my final #FluorescenceFriday post of 2024! 🔬
Here’s a kinetic showcasing pkMito dynamics and the heterogeneity of axonal mitochondria
I'm looking forward to all of the incredible microscopy from this community in 2025.
Happy Holidays everyone! ☃️❄️
Here’s a kinetic showcasing pkMito dynamics and the heterogeneity of axonal mitochondria
I'm looking forward to all of the incredible microscopy from this community in 2025.
Happy Holidays everyone! ☃️❄️
Some #MicroscopyMonday to kick off the week! 🔬⛄
I paired #lysosome and #mitochondria kinetics side-by-side to highlight their distinct axonal motility.
I thought this nicely shows the need for organelle-specific metrics when designing high-throughput screening assays.
#Neuroscience #CellBiology
I paired #lysosome and #mitochondria kinetics side-by-side to highlight their distinct axonal motility.
I thought this nicely shows the need for organelle-specific metrics when designing high-throughput screening assays.
#Neuroscience #CellBiology
December 16, 2024 at 3:04 PM
Some #MicroscopyMonday to kick off the week! 🔬⛄
I paired #lysosome and #mitochondria kinetics side-by-side to highlight their distinct axonal motility.
I thought this nicely shows the need for organelle-specific metrics when designing high-throughput screening assays.
#Neuroscience #CellBiology
I paired #lysosome and #mitochondria kinetics side-by-side to highlight their distinct axonal motility.
I thought this nicely shows the need for organelle-specific metrics when designing high-throughput screening assays.
#Neuroscience #CellBiology
The cell biology team at ALS Therapy Development Institute will be sharing some exciting work today at the 2024 International Symposium on ALS/MND. #endALS #Neuroscience #CellBiology
If you're here in Montreal, come chat with us during theme05: human cell biology and pathology (details below): 🧵
If you're here in Montreal, come chat with us during theme05: human cell biology and pathology (details below): 🧵
December 6, 2024 at 11:58 AM
The cell biology team at ALS Therapy Development Institute will be sharing some exciting work today at the 2024 International Symposium on ALS/MND. #endALS #Neuroscience #CellBiology
If you're here in Montreal, come chat with us during theme05: human cell biology and pathology (details below): 🧵
If you're here in Montreal, come chat with us during theme05: human cell biology and pathology (details below): 🧵
Reposted by TJ Krzystek
Important article that challenges the classic view of axons as simple tubes. To what extent they fluctuate in diameter is up for debate - see some thoughts in this commentary piece in @science.org. Exciting time for axon scientists!
December 2, 2024 at 10:33 AM
Important article that challenges the classic view of axons as simple tubes. To what extent they fluctuate in diameter is up for debate - see some thoughts in this commentary piece in @science.org. Exciting time for axon scientists!
Axonal mRNA studies always catch my attention.
Great preprint from the Redemann and Hermann labs: www.biorxiv.org/content/10.1...
". . . only very few studies have paid attention to the important roles of distinct mRNA expression profiles of the axon and soma . . ." #MoreAxonSeq #Neuroscience
Great preprint from the Redemann and Hermann labs: www.biorxiv.org/content/10.1...
". . . only very few studies have paid attention to the important roles of distinct mRNA expression profiles of the axon and soma . . ." #MoreAxonSeq #Neuroscience
December 2, 2024 at 12:46 PM
Axonal mRNA studies always catch my attention.
Great preprint from the Redemann and Hermann labs: www.biorxiv.org/content/10.1...
". . . only very few studies have paid attention to the important roles of distinct mRNA expression profiles of the axon and soma . . ." #MoreAxonSeq #Neuroscience
Great preprint from the Redemann and Hermann labs: www.biorxiv.org/content/10.1...
". . . only very few studies have paid attention to the important roles of distinct mRNA expression profiles of the axon and soma . . ." #MoreAxonSeq #Neuroscience
Sharing #FluorescenceFriday early before the food coma hits this weekend! 🦃
Here are longitudinal kinetics showing dose-dependent degeneration from an exogenous stressor: part of an assay developed for drug screening with iPSC-derived neurons. 🔬
#Microscopy #Neuroscience #Bioimaging #CellBiology
Here are longitudinal kinetics showing dose-dependent degeneration from an exogenous stressor: part of an assay developed for drug screening with iPSC-derived neurons. 🔬
#Microscopy #Neuroscience #Bioimaging #CellBiology
November 28, 2024 at 8:50 AM
Sharing #FluorescenceFriday early before the food coma hits this weekend! 🦃
Here are longitudinal kinetics showing dose-dependent degeneration from an exogenous stressor: part of an assay developed for drug screening with iPSC-derived neurons. 🔬
#Microscopy #Neuroscience #Bioimaging #CellBiology
Here are longitudinal kinetics showing dose-dependent degeneration from an exogenous stressor: part of an assay developed for drug screening with iPSC-derived neurons. 🔬
#Microscopy #Neuroscience #Bioimaging #CellBiology
Reposted by TJ Krzystek
I just created a Microscopy feed that tracks anything that has a cute 🔬 emoji on it as well as the simple #microscopy and #imaging tags. Enjoy!
bsky.app/profile/did:...
bsky.app/profile/did:...
November 23, 2024 at 7:48 PM
I just created a Microscopy feed that tracks anything that has a cute 🔬 emoji on it as well as the simple #microscopy and #imaging tags. Enjoy!
bsky.app/profile/did:...
bsky.app/profile/did:...
Reposted by TJ Krzystek
Interested in microscopy and/or image analysis? Check out my newest starter pack of super folks.(which also links to the previous, 150-awesome-full one! Both also link to other imaging-y starter packs as I find them)
go.bsky.app/MU8c2A2
go.bsky.app/MU8c2A2
November 14, 2024 at 2:00 PM
Interested in microscopy and/or image analysis? Check out my newest starter pack of super folks.(which also links to the previous, 150-awesome-full one! Both also link to other imaging-y starter packs as I find them)
go.bsky.app/MU8c2A2
go.bsky.app/MU8c2A2
Reposted by TJ Krzystek
ICYMI: The October FocalPlane features… webinar included talks from @helenajambor.bsky.social (‘How not to lie with image data’) and Kota Miura ('Reproducible bioimage analysis for ensuring #scientificintegrity').
Watch the recording at
bit.ly/4fyB2SU
#PublicationIntegrityWeek #COPE
Watch the recording at
bit.ly/4fyB2SU
#PublicationIntegrityWeek #COPE
FocalPlane features... reproducibility in imaging
YouTube video by The Company of Biologists
www.youtube.com
November 21, 2024 at 10:48 AM
ICYMI: The October FocalPlane features… webinar included talks from @helenajambor.bsky.social (‘How not to lie with image data’) and Kota Miura ('Reproducible bioimage analysis for ensuring #scientificintegrity').
Watch the recording at
bit.ly/4fyB2SU
#PublicationIntegrityWeek #COPE
Watch the recording at
bit.ly/4fyB2SU
#PublicationIntegrityWeek #COPE
Reposted by TJ Krzystek
My turn to create a starter pack for scientists using live imaging in their research! Of course, I’ve likely missed many—please let me know who else I should include. Thanks!
go.bsky.app/KYYr7xc
#liveimaging #livevellimaging #microscopy
go.bsky.app/KYYr7xc
#liveimaging #livevellimaging #microscopy
November 20, 2024 at 9:45 AM
My turn to create a starter pack for scientists using live imaging in their research! Of course, I’ve likely missed many—please let me know who else I should include. Thanks!
go.bsky.app/KYYr7xc
#liveimaging #livevellimaging #microscopy
go.bsky.app/KYYr7xc
#liveimaging #livevellimaging #microscopy
Some highlights from optimizing AAV transduction efficiency of an FL-reporter in iPSC-derived motor neurons.
Excited about striking a balance between sparse-labeling and 'painted' coverage.
#Neuroscience #Microscopy #CellBiology #AAV #iPSC
Excited about striking a balance between sparse-labeling and 'painted' coverage.
#Neuroscience #Microscopy #CellBiology #AAV #iPSC
November 20, 2024 at 1:21 PM
Some highlights from optimizing AAV transduction efficiency of an FL-reporter in iPSC-derived motor neurons.
Excited about striking a balance between sparse-labeling and 'painted' coverage.
#Neuroscience #Microscopy #CellBiology #AAV #iPSC
Excited about striking a balance between sparse-labeling and 'painted' coverage.
#Neuroscience #Microscopy #CellBiology #AAV #iPSC
Reposted by TJ Krzystek
Made a starter pack for microscopy and image analysis - please (self-)nominate additions!
go.bsky.app/Nwa91pC
go.bsky.app/Nwa91pC
November 6, 2024 at 4:32 PM
Made a starter pack for microscopy and image analysis - please (self-)nominate additions!
go.bsky.app/Nwa91pC
go.bsky.app/Nwa91pC
I feel the science-to-nonsense ratio is much better on this site:
Enjoy these jittery mitochondria sifting through axons of patient-derived motor neurons thanks to the pkMito probe from @spirochrome.com
#neuroscience #science #microscopy #bioimaging #mitochondria
Enjoy these jittery mitochondria sifting through axons of patient-derived motor neurons thanks to the pkMito probe from @spirochrome.com
#neuroscience #science #microscopy #bioimaging #mitochondria
October 19, 2024 at 7:19 PM
I feel the science-to-nonsense ratio is much better on this site:
Enjoy these jittery mitochondria sifting through axons of patient-derived motor neurons thanks to the pkMito probe from @spirochrome.com
#neuroscience #science #microscopy #bioimaging #mitochondria
Enjoy these jittery mitochondria sifting through axons of patient-derived motor neurons thanks to the pkMito probe from @spirochrome.com
#neuroscience #science #microscopy #bioimaging #mitochondria
Reposted by TJ Krzystek
I am excited to announce that the Journal of Cell Science will have a special issue on imaging cell architecture and dynamics, guest-edited by myself and Lucy Collinson. Consider this issue to publish your work.
Find out more: journals.biologists.com/jcs/pages/im...
Find out more: journals.biologists.com/jcs/pages/im...
October 9, 2023 at 4:07 PM
I am excited to announce that the Journal of Cell Science will have a special issue on imaging cell architecture and dynamics, guest-edited by myself and Lucy Collinson. Consider this issue to publish your work.
Find out more: journals.biologists.com/jcs/pages/im...
Find out more: journals.biologists.com/jcs/pages/im...
Fall is Elite 🍂😎
#EchoLake #MtCannon
#EchoLake #MtCannon
October 9, 2023 at 8:31 PM
Fall is Elite 🍂😎
#EchoLake #MtCannon
#EchoLake #MtCannon
Always a good feeling to find a positive hit in my axonal regeneration assay🔬 #neuroskyence
September 29, 2023 at 5:39 PM
Always a good feeling to find a positive hit in my axonal regeneration assay🔬 #neuroskyence
Once in a while,
glia (cyan) migrate out of organoids and completely steal the show from neurons (green) and their axons (magenta) 🔬
glia (cyan) migrate out of organoids and completely steal the show from neurons (green) and their axons (magenta) 🔬
September 18, 2023 at 5:45 PM
Once in a while,
glia (cyan) migrate out of organoids and completely steal the show from neurons (green) and their axons (magenta) 🔬
glia (cyan) migrate out of organoids and completely steal the show from neurons (green) and their axons (magenta) 🔬
Reposted by TJ Krzystek
September 9, 2023 at 3:19 PM