Laramie (Smith) Aközbek
            
            @laramiemckenna.bsky.social
          
          220 followers
          550 following
          20 posts
        
          Comparative & Evolutionary Genomics in Myricaceae + Fagaceae, Sex Chromosomes, and Centromeres — PhD Candidate in the Harkess Lab || #NSFGRFP fellow || B.S. in Plant Bio from @uga_plantbio || 🐞
      
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              Ming Tommy Tang
              @tommytang.bsky.social
          
              · Feb 15
        
        
        
            What is a Differentially Expressed Gene?
            The concept of ‘Differentially Expressed Genes’ (DEGs) is central to RNA-Seq studies, yet their identification suffers from reproducibility issues. This is largely a consequence of the inherent biological and technical variation that cannot be captured with small numbers of replicates. When thresholds for p-values and log2 fold changes are introduced, this variability can propagate an incomplete description of the data, leading to differing interpretations. Here, we compare traditional binary DEG classification with a rank-based method, grounded in Bayesian statistics, using a published yeast dataset comprising over 40 replicates. This analysis reveals how the choice of thresholds and number of replicates results in discrepancies between studies and potentially interesting genes being overlooked. Furthermore, by comparing wild-type with wild-type samples, we show how variability in gene expression can be mistaken for differential expression. Evaluating current practices for navigating 
          
            
            www.biorxiv.org
          
        
      
    
        
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      I’m an NSF panel reviewer that was scheduled to meet today. Just got notice that all NSF panels were canceled today. I reviewed some innovative proposals in support of students. Devastating if these scholars don’t get to do this work. For the love of science & students I hope this is just a delay.💔
    
  
        
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