Leah Smith
@leahmsmith.bsky.social
Research Fellow at University of Otago NZ interested in phages, biofilms, genomics, CRISPR & phage therapy
Reposted by Leah Smith
#NewResearch
Streptococcus thermophilus phages circumvent host CRISPR defences via AcrIIIA2, which complexes with enolase, a highly abundant glycolysis enzyme, to block phage RNA binding
#MicroSky #CRISPR 🦠
www.nature.com/articles/s41...
Streptococcus thermophilus phages circumvent host CRISPR defences via AcrIIIA2, which complexes with enolase, a highly abundant glycolysis enzyme, to block phage RNA binding
#MicroSky #CRISPR 🦠
www.nature.com/articles/s41...
A phage-encoded anti-CRISPR protein co-opts host enolase to prevent type III CRISPR immunity - Nature Microbiology
Streptococcus thermophilus phages circumvent host CRISPR defences via AcrIIIA2, which complexes with enolase, a highly abundant glycolysis enzyme, to block phage RNA binding.
www.nature.com
November 11, 2025 at 3:49 PM
#NewResearch
Streptococcus thermophilus phages circumvent host CRISPR defences via AcrIIIA2, which complexes with enolase, a highly abundant glycolysis enzyme, to block phage RNA binding
#MicroSky #CRISPR 🦠
www.nature.com/articles/s41...
Streptococcus thermophilus phages circumvent host CRISPR defences via AcrIIIA2, which complexes with enolase, a highly abundant glycolysis enzyme, to block phage RNA binding
#MicroSky #CRISPR 🦠
www.nature.com/articles/s41...
Reposted by Leah Smith
Today in @nature.com , we highlight how a cousin of CRISPR-Cas10, mCpol, establishes an evolutionary trap in anti-phage immune systems.
Check out @erinedoherty.bsky.social and my work from @doudna-lab.bsky.social lab here:
www.nature.com/articles/s41...
Check out @erinedoherty.bsky.social and my work from @doudna-lab.bsky.social lab here:
www.nature.com/articles/s41...
A miniature CRISPR–Cas10 enzyme confers immunity by inhibitory signalling - Nature
Panoptes, an anti-phage defence system against virus-mediated immune suppression, is revealed.
www.nature.com
October 1, 2025 at 5:57 PM
Today in @nature.com , we highlight how a cousin of CRISPR-Cas10, mCpol, establishes an evolutionary trap in anti-phage immune systems.
Check out @erinedoherty.bsky.social and my work from @doudna-lab.bsky.social lab here:
www.nature.com/articles/s41...
Check out @erinedoherty.bsky.social and my work from @doudna-lab.bsky.social lab here:
www.nature.com/articles/s41...
Nice write up by Kalani Gast and Rodolphe Barrangou previewing our latest paper 🧫 ✂️ 🧬 out now in @cp-cellhostmicrobe.bsky.social
All systems go: CRISPR crosstalk for enhanced immunity
Barrangou Previews papers led by @peterfineran.bsky.social & Meeske demonstrating CRISPR-Cas systems have unprecedented level of cooperative crosstalk between different subtypes, enabling primed spacer acquisition
www.cell.com/cell-host-mi...
Barrangou Previews papers led by @peterfineran.bsky.social & Meeske demonstrating CRISPR-Cas systems have unprecedented level of cooperative crosstalk between different subtypes, enabling primed spacer acquisition
www.cell.com/cell-host-mi...
All systems go: CRISPR crosstalk for enhanced immunity
In this issue of Cell Host & Microbe, companion manuscripts from Margolis & Meeske
and Smith & Fineran demonstrate that CRISPR-Cas systems have an unprecedented level
of cooperative crosstalk between ...
www.cell.com
September 15, 2025 at 9:02 PM
Nice write up by Kalani Gast and Rodolphe Barrangou previewing our latest paper 🧫 ✂️ 🧬 out now in @cp-cellhostmicrobe.bsky.social
Reposted by Leah Smith
Applied research is digging for gold, basic research is figuring out where to dig. Marsden used to fund it all. Each grant is rigorously assessed in 2 rounds by a panel of local & international experts. It is risky research, with potentially high rewards.
More cuts to the Marsden Fund. This time $20 million from the annual budget cut starting next year. This is on top of previous cuts by this government. They are trying to skip the 'priming the pump' step of public research as a driver of the economy. How well that is going to work?
#NZPOL
#NZPOL
Fewer Marsden Fund grants after government cuts
A major research fund has been forced to slash its grant allocation by more than $20 million next year.
www.rnz.co.nz
September 6, 2025 at 9:35 PM
Applied research is digging for gold, basic research is figuring out where to dig. Marsden used to fund it all. Each grant is rigorously assessed in 2 rounds by a panel of local & international experts. It is risky research, with potentially high rewards.
Reposted by Leah Smith
Can CRISPR-Cas systems target any phage? Yes, no, it depends? What does it depend on? The second paper from my PhD came out today in a special issue of Phil Trans B on the ecology and evolution of bacterial immune systems
doi.org/10.1098/rstb...
doi.org/10.1098/rstb...
Phage susceptibility to a minimal, modular synthetic CRISPR-Cas system in Pseudomonas aeruginosa is nutrient dependent | Philosophical Transactions of the Royal Society B: Biological Sciences
CRISPR-Cas systems can provide adaptive, heritable immunity to their prokaryotic hosts
against invading genetic material such as phages. It is clear that the importance
of acquiring CRISPR-Cas immunit...
doi.org
September 4, 2025 at 11:32 AM
Can CRISPR-Cas systems target any phage? Yes, no, it depends? What does it depend on? The second paper from my PhD came out today in a special issue of Phil Trans B on the ecology and evolution of bacterial immune systems
doi.org/10.1098/rstb...
doi.org/10.1098/rstb...
Reposted by Leah Smith
Stoked to finally have a preprint out for Phold, our tool that uses protein structural information to enhance phage genome annotation #phagesky 1/n
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Protein Structure Informed Bacteriophage Genome Annotation with Phold
Bacteriophage (phage) genome annotation is essential for understanding their functional potential and suitability for use as therapeutic agents. Here we introduce Phold, an annotation framework utilis...
www.biorxiv.org
August 8, 2025 at 7:11 AM
Stoked to finally have a preprint out for Phold, our tool that uses protein structural information to enhance phage genome annotation #phagesky 1/n
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
In a multi-CRISPR-Cas system host, @peterfineran.bsky.social & I found that type I priming promotes spacer 🧬 uptake & maintenance by a type III system & that multiple systems minimize phage escape ⬇️ @cp-cellhostmicrobe.bsky.social @otagomicroimmuno.bsky.social
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Type I CRISPR-Cas immunity primes type III spacer acquisition
CRISPR-Cas systems are diverse, with microbes harboring multiple classes and subtypes. Type I DNA-targeting and type III RNA-targeting systems often c…
www.sciencedirect.com
August 20, 2025 at 2:06 AM
In a multi-CRISPR-Cas system host, @peterfineran.bsky.social & I found that type I priming promotes spacer 🧬 uptake & maintenance by a type III system & that multiple systems minimize phage escape ⬇️ @cp-cellhostmicrobe.bsky.social @otagomicroimmuno.bsky.social
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Reposted by Leah Smith
Still open for applications! We are looking for prospective new faculty doing exciting research in virology or any vector borne diseases
The Wadsworth Center is expanding it's research focus on #virology /vector-borne diseases!
www.asmcareerconnections.org/job/research-scientist-5-g-31/78488664/
This is a unicorn assistant prof equivalent position with 12-month hard money salary and no teaching commitments (!)
Reposts appreciated 🙏
www.asmcareerconnections.org/job/research-scientist-5-g-31/78488664/
This is a unicorn assistant prof equivalent position with 12-month hard money salary and no teaching commitments (!)
Reposts appreciated 🙏
July 25, 2025 at 11:04 PM
Still open for applications! We are looking for prospective new faculty doing exciting research in virology or any vector borne diseases
Reposted by Leah Smith
Please share! 🦠
Workshop on Phage Therapy in Liverpool on 25th November organised by Stineke van Houte, @jojofoth.bsky.social, @brockhurstlab.bsky.social, & Edze Westra.
Limited FREE tickets & more info here: sites.exeter.ac.uk/vanhoutelab/...
#MicroSky
Workshop on Phage Therapy in Liverpool on 25th November organised by Stineke van Houte, @jojofoth.bsky.social, @brockhurstlab.bsky.social, & Edze Westra.
Limited FREE tickets & more info here: sites.exeter.ac.uk/vanhoutelab/...
#MicroSky
July 22, 2025 at 7:03 PM
Please share! 🦠
Workshop on Phage Therapy in Liverpool on 25th November organised by Stineke van Houte, @jojofoth.bsky.social, @brockhurstlab.bsky.social, & Edze Westra.
Limited FREE tickets & more info here: sites.exeter.ac.uk/vanhoutelab/...
#MicroSky
Workshop on Phage Therapy in Liverpool on 25th November organised by Stineke van Houte, @jojofoth.bsky.social, @brockhurstlab.bsky.social, & Edze Westra.
Limited FREE tickets & more info here: sites.exeter.ac.uk/vanhoutelab/...
#MicroSky
Reposted by Leah Smith
📣 New preprint alert!
Happy to share the first results from our effort to reannotate Pseudomonas #phage proteins of unknown function, using structural bioinformatics.
📄 doi.org/10.1101/2025...
Happy to share the first results from our effort to reannotate Pseudomonas #phage proteins of unknown function, using structural bioinformatics.
📄 doi.org/10.1101/2025...
Fold first, ask later: structure-informed function annotation of Pseudomonas phage proteins
Phages, the viruses of bacteria, harbor an incredibly diverse repertoire of proteins capable of manipulating their bacterial hosts, inspiring many medical and biotechnological applications. However, t...
doi.org
July 21, 2025 at 6:51 AM
📣 New preprint alert!
Happy to share the first results from our effort to reannotate Pseudomonas #phage proteins of unknown function, using structural bioinformatics.
📄 doi.org/10.1101/2025...
Happy to share the first results from our effort to reannotate Pseudomonas #phage proteins of unknown function, using structural bioinformatics.
📄 doi.org/10.1101/2025...
Reposted by Leah Smith
Next up from our lab - a ring nuclease with a novel fold which cleaves all cOA species involved in Type III CRISPR defence. It's also found in plasmid and phage genomes, where it presumably functions as an anti-CRISPR.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
July 18, 2025 at 7:53 AM
Next up from our lab - a ring nuclease with a novel fold which cleaves all cOA species involved in Type III CRISPR defence. It's also found in plasmid and phage genomes, where it presumably functions as an anti-CRISPR.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Great to see this comprehensive work on phage DNA arabinosylation & defense finally out! 🧬🚫✂️ A major collaborative effort & stellar PhD work by Marina Mahler, whom I had the pleasure of co-supervising at Otago 👩🏻🔬 @peterfineran.bsky.social @universityofotago.bsky.social @cellpress.bsky.social #mm
Phage DNA modifications evade defense
Phage DNA modification adds arabinose to cytosines via hydroxy linkage (5ara-hC) w/ potential double & triple arabinosylation. Arabinosylated phages are protected from DNA-targeting CRISPR-Cas & restriction modification
www.cell.com/cell-host-mi...
Phage DNA modification adds arabinose to cytosines via hydroxy linkage (5ara-hC) w/ potential double & triple arabinosylation. Arabinosylated phages are protected from DNA-targeting CRISPR-Cas & restriction modification
www.cell.com/cell-host-mi...
Phage arabinosyl-hydroxy-cytosine DNA modifications result in distinct evasion and sensitivity responses to phage defense systems
Mahler et al. describe a phage DNA modification that adds arabinose to cytosines via
a hydroxy linkage. This 5ara-hC modification can be further modified to double and
triple arabinosylation by phage-...
www.cell.com
June 26, 2025 at 10:44 PM
Great to see this comprehensive work on phage DNA arabinosylation & defense finally out! 🧬🚫✂️ A major collaborative effort & stellar PhD work by Marina Mahler, whom I had the pleasure of co-supervising at Otago 👩🏻🔬 @peterfineran.bsky.social @universityofotago.bsky.social @cellpress.bsky.social #mm