We are still accepting abstracts for selected short talks and posters. The abstract deadline has been extended to this Friday, 1st Aug. More information and registration here: www.lorneubiquitin.org .

📣📣 Lorne Ubiquitin – The Australian Ubiquitin Summit 📣📣 20-23 Nov 2025 in Lorne, Australia Join us this Nov for the first ubiquitin meeting in Australia. Submit your abstract by 18 July 2025 for oral and poster presentations: www.lorneubiquitin.org.
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We hope this will be useful for the field. A plasmid for expressing the constitutively active HOIL-1 variant is available via @addgene.bsky.social: www.addgene.org/229539/

Excited to share our latest work, now out in @lsajournal.org. we show that the E3 ligase HOIL-1 can ubiquitinate many different saccharides and describe a chemoenzymatic platform to generate ubiquitinated sugars as tools for studying non-proteinaceous ubiquitination.

For those interested in ubiquitinating their own sugars: our plasmid for bacterial expression of the constitutive HOIL-1 variant is now available via Addgene: www.addgene.org/229539/

The RBR E3 ubiquitin ligase HOIL-1 can ubiquitinate diverse non-proteinaceous substrates in vitro https://www.biorxiv.org/content/10.1101/2024.11.29.626046v1

Thanks to all the members of my lab as well as collaborators and co-authors in the Komander (WEHI), Goddard-Borger (WEHI) and Payne (Uni Sydney) labs.

www.biorxiv.org/content/10.1...

We hope that our protocol will enable us and others to generate various ubiquitinated saccharides as tools to further develop the non-proteinaceous ubiquitin field. A plasmid for bacterial expression of the HOIL-1 variant will be available at @addgene.bsky.social soon.

We utilise HOIL's broad saccharide substrate specificity to develop a simple protocol for the chemoenzymatic generation of preparative amounts of ubiquitinated saccharides. Our approach is vastly simplified by our engineered constitutively active HOIL-1 variant.

We find that HOIL-1 efficiently ubiquitinates hydroxyl groups in Ser and a wide variety of mono- and disaccharides. We show that HOIL-1's His510 residues is critical for this activity and that mutation of His510 to Ala switches HOIL-1 activity from hydroxyl groups to targeting amines.

HOIL-1 is an E3 ubiquitin ligase that can ubiquitinate non-proteinaceous substrates such as glycogen and maltose as first shown in work by @iankelsall.bsky.social. In our work, we systematically compare HOIL-1's in vitro activity against a number of amino acids (Lys, Ser, Thr) and saccharides.

Excited to share our latest preprint on @biorxivpreprint.bsky.social: "The RBR E3 ubiquitin ligase HOIL-1 can ubiquitinate diverse non-protein substrates in vitro".

www.biorxiv.org/content/10.1...

Check out this list of ubiquitin and Ubl profiles to follow. Repost if you want me to add you to it. Let’s create a good community here ☁️ go.bsky.app/3VPQofn