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@myosinactncrazy.bsky.social
930 followers 81 following 300 posts
#1 molecular motor,
cytoskeletal motors, cytoskeletal filaments, filament dynamics, cell migration, mechanobiology, morphogenesis
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rupress.org/jcb/article/...
High-resolution mapping of the actin fusion focus reveals myosin V–dependent formin transport for aster formation
Thomas et al. use centroid tracking to provide a live, 8-nm precision map of the actin fusion focus that underlies cell–cell fusion in Schizosaccharomyces
rupress.org
December 3, 2025 at 8:20 PM
Reposted
drmichaelway.bsky.social
SPIN90 and #YanCao strike again! First NPF to only activate a subset of Arp2/3 iso-complexes to regulate lamellipodial actin & protrusion efficiency. Great collaboration with @romet-jegou-lab.bsky.social #ManosMavrakis polarized fluorescence microscopy rocks 🤘💥🎉
www.biorxiv.org/content/10.6...
SPIN90 modulates the architecture of lamellipodial actin in an ARPC5L dependent fashion.
When stimulated by nucleation-promoting factors such as WAVE, the Arp2/3 complex generates branched actin networks. In contrast, when activated by SPIN90, the Arp2/3 complex generates linear actin fil...
www.biorxiv.org
December 3, 2025 at 8:30 AM
www.biorxiv.org/content/10.6...
Structural mechanisms of drebrin-mediated F-actin network modulation
Drebrin modulates F-actin networks and links them to other intracellular components, regulating crucial processes including neuritogenesis, synaptic plasticity, virus internalisation and cancer invasi...
www.biorxiv.org
December 3, 2025 at 12:26 PM
www.cell.com/current-biol...
Cell contractility: Myosin-II-mediated contraction of fast-crawling amoebae
Myosin-II-based cell contractility is essential for cell motility and cytokinesis in species within the Amorphea eukaryotic supergroup. New work demonstrates myosin-II-based contractility in evolution...
www.cell.com
December 3, 2025 at 12:20 PM
Reposted
drubin.bsky.social
Today, our animation synthesizing decades of research on actin-mediated endocytosis in budding yeast was published:
journals.biologists.com/jcs/article/...

The result of a fantastic Iwasa-Drubin lab collaboration.

@margotriggi.bsky.social @jiwasa.bsky.social
movie.biologists.com/video/10.124...
December 2, 2025 at 9:02 PM
www.nature.com/articles/s41...
Assaying the myosin super-relaxed state across muscle types, cells and proteins for understanding muscle biology and use in drug discovery - Nature Protocols
The Mant-ATP assay is a simple and accessible means for rapid quantitative assessment of the ratio of super-relaxed myosin to disordered relaxed myosin. This protocol provides a standardized methodolo...
www.nature.com
December 2, 2025 at 8:06 PM
Reposted
oliverio.bsky.social
Thanks nature news for featuring our recent preprint on 𝘐𝘯𝘤𝘦𝘯𝘥𝘪𝘢𝘮𝘰𝘦𝘣𝘢 led by @hbrappap.bsky.social

(check out preprint here: doi.org/10.1101/2025...) #protistsonsky
December 2, 2025 at 3:15 PM
www.biorxiv.org/content/10.1...
Dynamic protrusions mediate unique crawling motility in Asgard Archaea (Promethearchaeota)
Crawling motility is a hallmark of eukaryotic cells and requires a dynamic actin cytoskeleton, regulated adhesion, and spatially organized signalling pathways. Asgard archaea (phylum Promethearchaeota...
www.biorxiv.org
December 2, 2025 at 1:30 PM
www.nature.com/articles/s41...
Nuclear actin and DNA replication stress regulate telomere maintenance by telomerase - Nature Communications
Telomerase recruitment to telomeres is a tightly regulated process which is stimulated by replication stress. Here, the authors identify that nuclear filamentous actin is important for interaction bet...
www.nature.com
December 2, 2025 at 1:28 PM
www.nature.com/articles/s41...
Measuring cellular force using DNA-based tension probes: from ensemble to single-molecule studies - Nature Protocols
The authors present a protocol for the use of two advanced DNA-based tension probes, the reversible shearing DNA-based tension probe (RSDTP) and ForceChrono probe, which provide powerful tools for stu...
www.nature.com
November 29, 2025 at 2:34 PM
www.cell.com/current-biol...
Calponin isoforms define the cell-type-specific organization and dynamics of actomyosin bundles
Kokate et al. show that calponins regulate the architecture of actomyosin bundles. Their loss results in abnormal distribution of α-actinin, reduced thickness of actomyosin bundles, and impaired cell ...
www.cell.com
November 28, 2025 at 3:22 PM
www.cell.com/cell-reports...
Actin cage for the synaptic vesicle liquid phase
Brodin and Shupliakov demonstrate that synaptic activity triggers the formation of an actin cage that encapsulates the synaptic vesicle cluster. Disruption of this cage leads to the detachment of the ...
www.cell.com
November 27, 2025 at 11:35 PM
Reposted
mag2art.bsky.social
Today’s data drop from our alpha-actinin-4 paper!
1A: Cardiac myocytes don’t just express muscle alpha-actinin (ACTN2)… they also express ACTN4; a “non-muscle” paralog. 1B: ACTN4 is sitting right at the Z-disc, shoulder-to-shoulder with ACTN2.
www.ahajournals.org/doi/10.1161/...
November 27, 2025 at 3:41 PM
Reposted
juanmagararc.bsky.social
New paper from the @rouxlab.bsky.social on Nature Communications! We study how membrane tension is spatially organized in cells. Using the mechanosensitive probe Flipper-TR to visualize tension across the plasma membrane of adherent cells and to dissect the conditions needed for a gradient to happen
November 27, 2025 at 2:14 PM
Reposted
ijmonod.bsky.social
📣 2nd meeting of France-BioImaging Mechanobiology Working Group

📆 March 26-27th, 2026
📍Institut Jacques Monod, Buffon amphitheater

Registration are open until December 31st 🔗 buff.ly/cVzaoId

@upcite.bsky.social @sbcf.bsky.social @gdraqv.bsky.social @cnrsbiologie.bsky.social @psl-univ.bsky.social
2nd meeting of France-BioImaging Mechanobiology Working Group
The second meeting of the FBI Mechanobiology WG will take place on the 26 and 27 of March 2026 at the Institut de Biologie Paris Seine and the Institut Jacques Monod in Paris. The programme will in…
france-bioimaging.org
November 27, 2025 at 3:26 PM
Effect of Load on Non-Muscle Myosin 2 Paralog Filaments in a Biomimetic Contractile Actin Array

onlinelibrary.wiley.com/doi/10.1002/...
Effect of Load on Non‐Muscle Myosin 2 Paralog Filaments in a Biomimetic Contractile Actin Array
The main paralogs of non-muscle myosin 2 are studied on a micropatterned array of anti-parallel actin filaments. This sarcomere-like platform reveals myosin's load-dependent behavior, and the resulti...
onlinelibrary.wiley.com
November 27, 2025 at 12:59 PM
Reposted
ichikawa-lab.bsky.social
Our paper on dynein-2's track recognition mechanism is now published in The EMBO Journal @embojournal.org!!

Cryo-ET and MD simulations reveal that dynein-2 is tuned for binding to the A-tubule of the ciliary doublet | The EMBO Journal www.embopress.org/doi/full/10....
Cryo-ET and MD simulations reveal that dynein-2 is tuned for binding to the A-tubule of the ciliary doublet | The EMBO Journal
imageimageDynein-2 is a motor protein essential for retrograde intraflagellar transport (IFT) on the A-tubule of ciliary doublet microtubules. Here, biochemical and cryo-ET analyses demonstrate that d...
www.embopress.org
November 26, 2025 at 4:57 PM
www.pnas.org/doi/10.1073/...
Capped high-force integrin bond lifetimes and spacing-tuned binding frequency drive rapid fibroblast migration | PNAS
Cell migration relies on balancing focal adhesion (FA) stability—necessary for traction generation—and turnover—essential for forward translocation...
www.pnas.org
November 26, 2025 at 12:01 PM
Reposted
hbrappap.bsky.social
So happy to announce our new preprint, “A geothermal amoeba sets a new upper temperature limit for eukaryotes.” We cultured a novel amoeba from Lassen Volcanic NP (CA, USA) that divides at 63°C (145°F) 🔥 - a new record for euk growth!
#protistsonsky 🧵
November 25, 2025 at 8:41 PM
www.biorxiv.org/content/10.1...
Form and function of actin impacts actin health and aging.
The actin cytoskeleton is a fundamental and highly conserved structure that functions in diverse cellular processes, yet its direct contribution to organismal aging remains unclear. Here, we systemati...
www.biorxiv.org
November 23, 2025 at 3:28 PM
interesting work revealing differences between evolutionary distant actins!

"..... ADP-actin filaments depolymerize faster and are mechanically more flexible in Saccharomyces cerevisiae compared to Schizosaccharomyces pombe. "

www.biorxiv.org/content/10.1...
Rapid aging and disassembly of actin filaments from two evolutionary distant yeasts
Actin is an essential cytoskeletal protein that is extremely well conserved across the tree of life. Similarities and differences in the way actin from different species self-assembles into filaments ...
www.biorxiv.org
November 22, 2025 at 1:31 PM
www.biorxiv.org/content/10.1...
Enhanced Processivity and Collective Force Production of Kinesin-1 at Low Radial Forces
Kinesin-1 is a robust motor that carries intracellular cargos towards the plus ends of microtubules. However, optical trapping studies reported that kinesin-1 is a slippery motor that quickly detaches...
www.biorxiv.org
November 22, 2025 at 1:25 PM
Reposted
ryotaiino.bsky.social
Active fluctuations of cytoplasmic actomyosin networks facilitate dynein-driven transport: iScience www.cell.com/iscience/ful...
Active fluctuations of cytoplasmic actomyosin networks facilitate dynein-driven transport
Inside cells, molecular motors transport cargo through a highly crowded cytoplasmic environment. While such an environment is assumed to hinder transport, its precise effect remains unclear. Here, we ...
www.cell.com
November 22, 2025 at 1:39 AM
www.pnas.org/doi/10.1073/...
ATP-dependent actin barbed-end fluctuations at steady state | PNAS
Actin networks in cells are dynamic and constantly turning over as actin subunits exchange between monomer and polymer pools. Understanding these d...
www.pnas.org
November 21, 2025 at 12:12 PM
elifesciences.org/reviewed-pre...
Kinesin-1 conformational dynamics are controlled by a cargo-sensitive TPR switch
elifesciences.org
November 21, 2025 at 3:16 AM