Paula Ramiro-Martínez
@paularamiro.bsky.social
Predoctoral researcher at evodynamics lab in Madrid.
Bacterial and plasmid evolution 🫧🧬💻
Bacterial and plasmid evolution 🫧🧬💻
Pinned
Universal rules govern plasmid copy number - Nature Communications
Plasmids exhibit a broad range of sizes and copies per cell, and these two parameters appear to be negatively correlated. Here, Ramiro-Martínez et al. analyse the copy number of thousands of diverse b...
doi.org
Curious about plasmid biology? Our latest paper is out now in Nature Communications! 🚨
doi.org/10.1038/s414...
We analyzed thousands of diverse bacterial plasmids to shed light for the first time on a key aspect of plasmid biology: plasmid copy number. 1/7 👇
doi.org/10.1038/s414...
We analyzed thousands of diverse bacterial plasmids to shed light for the first time on a key aspect of plasmid biology: plasmid copy number. 1/7 👇
Reposted by Paula Ramiro-Martínez
A pleasure and an honour to have received this prestigious grant with my admired colleagues and friends @sanmillan.bsky.social and @asanchezlab.bsky.social . More about AMR under plasmid lens!. My sincere gratitude to @caixaresearch.bsky.social
@microryc.bsky.social @esgem-sg.bsky.social
@microryc.bsky.social @esgem-sg.bsky.social
November 21, 2025 at 5:19 AM
A pleasure and an honour to have received this prestigious grant with my admired colleagues and friends @sanmillan.bsky.social and @asanchezlab.bsky.social . More about AMR under plasmid lens!. My sincere gratitude to @caixaresearch.bsky.social
@microryc.bsky.social @esgem-sg.bsky.social
@microryc.bsky.social @esgem-sg.bsky.social
Reposted by Paula Ramiro-Martínez
A plasmid golden ratio? 🧬
Plasmid copy number ≈ 2.5% of chromosome size—consistent across bacterial species!
pmc.ncbi.nlm.nih.gov/articles/PMC... @jerorb.bsky.social
🧪 #microbesky
Plasmid copy number ≈ 2.5% of chromosome size—consistent across bacterial species!
pmc.ncbi.nlm.nih.gov/articles/PMC... @jerorb.bsky.social
🧪 #microbesky
September 9, 2025 at 3:54 AM
A plasmid golden ratio? 🧬
Plasmid copy number ≈ 2.5% of chromosome size—consistent across bacterial species!
pmc.ncbi.nlm.nih.gov/articles/PMC... @jerorb.bsky.social
🧪 #microbesky
Plasmid copy number ≈ 2.5% of chromosome size—consistent across bacterial species!
pmc.ncbi.nlm.nih.gov/articles/PMC... @jerorb.bsky.social
🧪 #microbesky
Reposted by Paula Ramiro-Martínez
In episode 337 TWiM explains a study that examines pathogen presence in ancient humans and concludes that zoonoses emerged 6500 years ago with the domestication of livestock, and determination of universal rules that govern plasmid copy number. This week's special guest: @markowenmartin.bsky.social
TWiM 337: Lifestyles of the plasmids
YouTube video by MicrobeTV
youtu.be
August 19, 2025 at 6:52 PM
In episode 337 TWiM explains a study that examines pathogen presence in ancient humans and concludes that zoonoses emerged 6500 years ago with the domestication of livestock, and determination of universal rules that govern plasmid copy number. This week's special guest: @markowenmartin.bsky.social
Reposted by Paula Ramiro-Martínez
This work is finally published! 🥳🧬
Plasmids are associated with very variable fitness costs in their different bacterial hosts. But, what is the contribution of each of the plasmid-genes in these host-specific effects? Study led by
@jorgesastred.bsky.social, @sanmillan.bsky.social and myself! 1/14
Plasmids are associated with very variable fitness costs in their different bacterial hosts. But, what is the contribution of each of the plasmid-genes in these host-specific effects? Study led by
@jorgesastred.bsky.social, @sanmillan.bsky.social and myself! 1/14
Dissecting pOXA-48 fitness effects in clinical Enterobacterales using plasmid-wide CRISPRi screens
Nature Communications - This study investigates the effects of the carbapenem resistance plasmid pOXA-48 in clinical enterobacteria. Using CRISPRi screens, the authors revealed that the...
rdcu.be
August 20, 2025 at 1:25 PM
This work is finally published! 🥳🧬
Plasmids are associated with very variable fitness costs in their different bacterial hosts. But, what is the contribution of each of the plasmid-genes in these host-specific effects? Study led by
@jorgesastred.bsky.social, @sanmillan.bsky.social and myself! 1/14
Plasmids are associated with very variable fitness costs in their different bacterial hosts. But, what is the contribution of each of the plasmid-genes in these host-specific effects? Study led by
@jorgesastred.bsky.social, @sanmillan.bsky.social and myself! 1/14
Reposted by Paula Ramiro-Martínez
Our new manuscript is out! A bit of everything cool:
Plasmids ✅
Insertion Sequences ✅
AMR Evolution ✅
Microbial Communities ✅
Databases analyses ✅
Mathematical modeling ✅
See the scientific thread below of Jorge Sastre, who has brilliantly led this work with @palomarodera.bsky.social
Plasmids ✅
Insertion Sequences ✅
AMR Evolution ✅
Microbial Communities ✅
Databases analyses ✅
Mathematical modeling ✅
See the scientific thread below of Jorge Sastre, who has brilliantly led this work with @palomarodera.bsky.social
New paper out! 🔈🔈📣📣
Plasmids promote antimicrobial resistance through Insertion Sequence-mediated gene inactivation.
Combining experimental and computational approaches, we unveil how two of the most prevalent bacterial MGE accelerate the evolution of AMR. 🧵👇🏻
www.biorxiv.org/content/10.1...
Plasmids promote antimicrobial resistance through Insertion Sequence-mediated gene inactivation.
Combining experimental and computational approaches, we unveil how two of the most prevalent bacterial MGE accelerate the evolution of AMR. 🧵👇🏻
www.biorxiv.org/content/10.1...
Plasmids promote antimicrobial resistance through Insertion Sequence-mediated gene inactivation
Antimicrobial Resistance (AMR) is a major threat to public health. Plasmids are mobile genetic elements that can rapidly spread across bacterial populations, promoting the dissemination of AMR genes i...
www.biorxiv.org
August 13, 2025 at 3:04 PM
Our new manuscript is out! A bit of everything cool:
Plasmids ✅
Insertion Sequences ✅
AMR Evolution ✅
Microbial Communities ✅
Databases analyses ✅
Mathematical modeling ✅
See the scientific thread below of Jorge Sastre, who has brilliantly led this work with @palomarodera.bsky.social
Plasmids ✅
Insertion Sequences ✅
AMR Evolution ✅
Microbial Communities ✅
Databases analyses ✅
Mathematical modeling ✅
See the scientific thread below of Jorge Sastre, who has brilliantly led this work with @palomarodera.bsky.social
Reposted by Paula Ramiro-Martínez
New paper out! 🔈🔈📣📣
Plasmids promote antimicrobial resistance through Insertion Sequence-mediated gene inactivation.
Combining experimental and computational approaches, we unveil how two of the most prevalent bacterial MGE accelerate the evolution of AMR. 🧵👇🏻
www.biorxiv.org/content/10.1...
Plasmids promote antimicrobial resistance through Insertion Sequence-mediated gene inactivation.
Combining experimental and computational approaches, we unveil how two of the most prevalent bacterial MGE accelerate the evolution of AMR. 🧵👇🏻
www.biorxiv.org/content/10.1...
Plasmids promote antimicrobial resistance through Insertion Sequence-mediated gene inactivation
Antimicrobial Resistance (AMR) is a major threat to public health. Plasmids are mobile genetic elements that can rapidly spread across bacterial populations, promoting the dissemination of AMR genes i...
www.biorxiv.org
August 13, 2025 at 6:26 AM
New paper out! 🔈🔈📣📣
Plasmids promote antimicrobial resistance through Insertion Sequence-mediated gene inactivation.
Combining experimental and computational approaches, we unveil how two of the most prevalent bacterial MGE accelerate the evolution of AMR. 🧵👇🏻
www.biorxiv.org/content/10.1...
Plasmids promote antimicrobial resistance through Insertion Sequence-mediated gene inactivation.
Combining experimental and computational approaches, we unveil how two of the most prevalent bacterial MGE accelerate the evolution of AMR. 🧵👇🏻
www.biorxiv.org/content/10.1...
Reposted by Paula Ramiro-Martínez
🚨🚨 New paper in @narjournal.bsky.social! 🍾
Excludons are pairs of overlapping genes that block each other’s expression (basically, reverse operons).
We built a tool to identify them in bacterial genomes using transcriptomic data, in an awesome collab led by Iñigo Lasa and Álvaro San Martín.
👇
Excludons are pairs of overlapping genes that block each other’s expression (basically, reverse operons).
We built a tool to identify them in bacterial genomes using transcriptomic data, in an awesome collab led by Iñigo Lasa and Álvaro San Martín.
👇
ExcludonFinder: mapping transcriptional overlaps between neighboring genes
Abstract. Bacteria regulate neighboring genes via overlapping transcription in untranslated regions (UTRs), forming excludons. This overlap leads to transc
academic.oup.com
July 25, 2025 at 9:26 PM
🚨🚨 New paper in @narjournal.bsky.social! 🍾
Excludons are pairs of overlapping genes that block each other’s expression (basically, reverse operons).
We built a tool to identify them in bacterial genomes using transcriptomic data, in an awesome collab led by Iñigo Lasa and Álvaro San Martín.
👇
Excludons are pairs of overlapping genes that block each other’s expression (basically, reverse operons).
We built a tool to identify them in bacterial genomes using transcriptomic data, in an awesome collab led by Iñigo Lasa and Álvaro San Martín.
👇
Reposted by Paula Ramiro-Martínez
Had an amazing time at #FEMS2025 last week, where I presented our latest findings on collateral sensitivity mediated by beta-lactamases ☯️ Big thanks to everyone who came by! So proud of my colleagues for their brilliant talks and posters ✨
July 23, 2025 at 6:43 AM
Had an amazing time at #FEMS2025 last week, where I presented our latest findings on collateral sensitivity mediated by beta-lactamases ☯️ Big thanks to everyone who came by! So proud of my colleagues for their brilliant talks and posters ✨
Reposted by Paula Ramiro-Martínez
Do plasmids evolve faster 🐇, slower 🐢, or just like chromosomes 🧬?
In our new paper, we tackled this question using theory, simulations, bioinformatics, and experiments!
👇 Check out all the details in Paula’s thread!
Hint: 🐇 (most of the time)
In our new paper, we tackled this question using theory, simulations, bioinformatics, and experiments!
👇 Check out all the details in Paula’s thread!
Hint: 🐇 (most of the time)
July 22, 2025 at 3:05 PM
Do plasmids evolve faster 🐇, slower 🐢, or just like chromosomes 🧬?
In our new paper, we tackled this question using theory, simulations, bioinformatics, and experiments!
👇 Check out all the details in Paula’s thread!
Hint: 🐇 (most of the time)
In our new paper, we tackled this question using theory, simulations, bioinformatics, and experiments!
👇 Check out all the details in Paula’s thread!
Hint: 🐇 (most of the time)
Reposted by Paula Ramiro-Martínez
The must-read of this Summer! 🤩 Congrats to all the authors for this beautiful work 🥳
New paper alert! 🚨
Plasmids promote bacterial evolution through a copy number-driven increase in mutation rate.
We combine theory, simulations, experimental evolution, and bioinformatics to demonstrate that mutation rates scale with plasmid copy number.
Let's dive in! 🧵👇
Plasmids promote bacterial evolution through a copy number-driven increase in mutation rate.
We combine theory, simulations, experimental evolution, and bioinformatics to demonstrate that mutation rates scale with plasmid copy number.
Let's dive in! 🧵👇
Plasmids promote bacterial evolution through a copy number-driven increase in mutation rate
Plasmids are autonomously replicating DNA molecules that stably coexist with chromosomes in bacterial cells. These genetic elements drive horizontal gene transfer and play a fundamental role in bacter...
www.biorxiv.org
July 22, 2025 at 10:28 AM
The must-read of this Summer! 🤩 Congrats to all the authors for this beautiful work 🥳
New paper alert! 🚨
Plasmids promote bacterial evolution through a copy number-driven increase in mutation rate.
We combine theory, simulations, experimental evolution, and bioinformatics to demonstrate that mutation rates scale with plasmid copy number.
Let's dive in! 🧵👇
Plasmids promote bacterial evolution through a copy number-driven increase in mutation rate.
We combine theory, simulations, experimental evolution, and bioinformatics to demonstrate that mutation rates scale with plasmid copy number.
Let's dive in! 🧵👇
Plasmids promote bacterial evolution through a copy number-driven increase in mutation rate
Plasmids are autonomously replicating DNA molecules that stably coexist with chromosomes in bacterial cells. These genetic elements drive horizontal gene transfer and play a fundamental role in bacter...
www.biorxiv.org
July 22, 2025 at 9:38 AM
New paper alert! 🚨
Plasmids promote bacterial evolution through a copy number-driven increase in mutation rate.
We combine theory, simulations, experimental evolution, and bioinformatics to demonstrate that mutation rates scale with plasmid copy number.
Let's dive in! 🧵👇
Plasmids promote bacterial evolution through a copy number-driven increase in mutation rate.
We combine theory, simulations, experimental evolution, and bioinformatics to demonstrate that mutation rates scale with plasmid copy number.
Let's dive in! 🧵👇
Reposted by Paula Ramiro-Martínez
Plasmids promote bacterial evolution through a copy number-driven increase in mutation rate https://www.biorxiv.org/content/10.1101/2025.07.21.665944v1
July 22, 2025 at 7:18 AM
Plasmids promote bacterial evolution through a copy number-driven increase in mutation rate https://www.biorxiv.org/content/10.1101/2025.07.21.665944v1
Reposted by Paula Ramiro-Martínez
This is a legitimately incredible result that hints at something fundamental about plasmids that we don’t yet understand
🚨🚨New paper out in @natcomms.nature.com!!
Come for the first large-scale analysis of plasmid copy number across species,
stay for one of the most intriguing results of my lab: universal scaling laws in plasmid biology! 📈🧬
👉 www.nature.com/articles/s41...
Come for the first large-scale analysis of plasmid copy number across species,
stay for one of the most intriguing results of my lab: universal scaling laws in plasmid biology! 📈🧬
👉 www.nature.com/articles/s41...
Universal rules govern plasmid copy number - Nature Communications
Plasmids exhibit a broad range of sizes and copies per cell, and these two parameters appear to be negatively correlated. Here, Ramiro-Martínez et al. analyse the copy number of thousands of diverse b...
www.nature.com
July 20, 2025 at 4:27 PM
This is a legitimately incredible result that hints at something fundamental about plasmids that we don’t yet understand
Reposted by Paula Ramiro-Martínez
Super proud of the lab for their outstanding presentations at #FEMS2025 in Milano!
Science is better when you work with such a stellar team!🚀✨
@femsmicro.org
Science is better when you work with such a stellar team!🚀✨
@femsmicro.org
July 17, 2025 at 8:21 PM
Super proud of the lab for their outstanding presentations at #FEMS2025 in Milano!
Science is better when you work with such a stellar team!🚀✨
@femsmicro.org
Science is better when you work with such a stellar team!🚀✨
@femsmicro.org
Reposted by Paula Ramiro-Martínez
It was an honor and a joy to receive the National Research Prize for Young (!) Researchers. The highlight? My grandma greeting the King of Spain.
What a day!
www.youtube.com/watch?v=oSQP...
What a day!
www.youtube.com/watch?v=oSQP...
July 3, 2025 at 7:52 PM
It was an honor and a joy to receive the National Research Prize for Young (!) Researchers. The highlight? My grandma greeting the King of Spain.
What a day!
www.youtube.com/watch?v=oSQP...
What a day!
www.youtube.com/watch?v=oSQP...
Reposted by Paula Ramiro-Martínez
Oh this is fantastic 🦠🧫
Curious about plasmid biology? Our latest paper is out now in Nature Communications! 🚨
doi.org/10.1038/s414...
We analyzed thousands of diverse bacterial plasmids to shed light for the first time on a key aspect of plasmid biology: plasmid copy number. 1/7 👇
doi.org/10.1038/s414...
We analyzed thousands of diverse bacterial plasmids to shed light for the first time on a key aspect of plasmid biology: plasmid copy number. 1/7 👇
Universal rules govern plasmid copy number - Nature Communications
Plasmids exhibit a broad range of sizes and copies per cell, and these two parameters appear to be negatively correlated. Here, Ramiro-Martínez et al. analyse the copy number of thousands of diverse b...
doi.org
July 2, 2025 at 2:19 PM
Oh this is fantastic 🦠🧫
Reposted by Paula Ramiro-Martínez
If you're into plasmid biology, you can't miss this back-to-back by two inspiring teams!
🚨🚨New paper out in @natcomms.nature.com!!
Come for the first large-scale analysis of plasmid copy number across species,
stay for one of the most intriguing results of my lab: universal scaling laws in plasmid biology! 📈🧬
👉 www.nature.com/articles/s41...
Come for the first large-scale analysis of plasmid copy number across species,
stay for one of the most intriguing results of my lab: universal scaling laws in plasmid biology! 📈🧬
👉 www.nature.com/articles/s41...
Universal rules govern plasmid copy number - Nature Communications
Plasmids exhibit a broad range of sizes and copies per cell, and these two parameters appear to be negatively correlated. Here, Ramiro-Martínez et al. analyse the copy number of thousands of diverse b...
www.nature.com
July 2, 2025 at 3:51 PM
If you're into plasmid biology, you can't miss this back-to-back by two inspiring teams!
Reposted by Paula Ramiro-Martínez
🚨🚨New paper out in @natcomms.nature.com!!
Come for the first large-scale analysis of plasmid copy number across species,
stay for one of the most intriguing results of my lab: universal scaling laws in plasmid biology! 📈🧬
👉 www.nature.com/articles/s41...
Come for the first large-scale analysis of plasmid copy number across species,
stay for one of the most intriguing results of my lab: universal scaling laws in plasmid biology! 📈🧬
👉 www.nature.com/articles/s41...
Universal rules govern plasmid copy number - Nature Communications
Plasmids exhibit a broad range of sizes and copies per cell, and these two parameters appear to be negatively correlated. Here, Ramiro-Martínez et al. analyse the copy number of thousands of diverse b...
www.nature.com
July 2, 2025 at 11:08 AM
🚨🚨New paper out in @natcomms.nature.com!!
Come for the first large-scale analysis of plasmid copy number across species,
stay for one of the most intriguing results of my lab: universal scaling laws in plasmid biology! 📈🧬
👉 www.nature.com/articles/s41...
Come for the first large-scale analysis of plasmid copy number across species,
stay for one of the most intriguing results of my lab: universal scaling laws in plasmid biology! 📈🧬
👉 www.nature.com/articles/s41...
Curious about plasmid biology? Our latest paper is out now in Nature Communications! 🚨
doi.org/10.1038/s414...
We analyzed thousands of diverse bacterial plasmids to shed light for the first time on a key aspect of plasmid biology: plasmid copy number. 1/7 👇
doi.org/10.1038/s414...
We analyzed thousands of diverse bacterial plasmids to shed light for the first time on a key aspect of plasmid biology: plasmid copy number. 1/7 👇
Universal rules govern plasmid copy number - Nature Communications
Plasmids exhibit a broad range of sizes and copies per cell, and these two parameters appear to be negatively correlated. Here, Ramiro-Martínez et al. analyse the copy number of thousands of diverse b...
doi.org
July 2, 2025 at 10:07 AM
Curious about plasmid biology? Our latest paper is out now in Nature Communications! 🚨
doi.org/10.1038/s414...
We analyzed thousands of diverse bacterial plasmids to shed light for the first time on a key aspect of plasmid biology: plasmid copy number. 1/7 👇
doi.org/10.1038/s414...
We analyzed thousands of diverse bacterial plasmids to shed light for the first time on a key aspect of plasmid biology: plasmid copy number. 1/7 👇
Reposted by Paula Ramiro-Martínez
New mini-review on the respiratory microbiome and its contribution to human health from biotechnology 🫁🦠
@tmcoque.bsky.social @microbiotech.bsky.social
enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/...
@tmcoque.bsky.social @microbiotech.bsky.social
enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/...
The Respiratory Tract Microbiome and Human Health
The respiratory tract microbiome (RTM) trains the immune system and protects against infections. Changes in the RTM are linked to various respiratory diseases. High-throughput methodologies and compu...
enviromicro-journals.onlinelibrary.wiley.com
April 29, 2025 at 3:33 PM
New mini-review on the respiratory microbiome and its contribution to human health from biotechnology 🫁🦠
@tmcoque.bsky.social @microbiotech.bsky.social
enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/...
@tmcoque.bsky.social @microbiotech.bsky.social
enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/...
Reposted by Paula Ramiro-Martínez
Happy International Day of Women and Girls in Science 👩🔬🧬 #womeninscience #girlsinscience
February 11, 2025 at 8:29 AM
Happy International Day of Women and Girls in Science 👩🔬🧬 #womeninscience #girlsinscience
Reposted by Paula Ramiro-Martínez
Just migrated here from twitter! Hello everyone.
Also, I've just put up a super simple (and free) to use APP to streamline experiment design and result analysis of 96 (or 384) wells plate experiments for my lab pals.
You can try it here: rosettier.streamlit.app
Feedback is very welcome 😁!
Also, I've just put up a super simple (and free) to use APP to streamline experiment design and result analysis of 96 (or 384) wells plate experiments for my lab pals.
You can try it here: rosettier.streamlit.app
Feedback is very welcome 😁!
Rosettier
A free, user-friendly web application for planning 96 or 384-well plate experiments. Rosettier si...
rosettier.streamlit.app
January 28, 2025 at 2:39 PM
Just migrated here from twitter! Hello everyone.
Also, I've just put up a super simple (and free) to use APP to streamline experiment design and result analysis of 96 (or 384) wells plate experiments for my lab pals.
You can try it here: rosettier.streamlit.app
Feedback is very welcome 😁!
Also, I've just put up a super simple (and free) to use APP to streamline experiment design and result analysis of 96 (or 384) wells plate experiments for my lab pals.
You can try it here: rosettier.streamlit.app
Feedback is very welcome 😁!
Reposted by Paula Ramiro-Martínez
Really beautiful day receiving the “Margarita Salas” Award from the Madrid Region!
Really proud to share it with my colleagues, friends and family!!
Really proud to share it with my colleagues, friends and family!!
January 21, 2025 at 5:49 PM
Really beautiful day receiving the “Margarita Salas” Award from the Madrid Region!
Really proud to share it with my colleagues, friends and family!!
Really proud to share it with my colleagues, friends and family!!