The transcription factor CarD facilitates the activation of transcription in many bacteria and in Rhodobacter sphaeroides, CarD compensates for suboptimal promoter DNA sequences. Here, the authors sho...

Desiccation tolerance is central to the pathogenic success of the opportunistic pathogen Acinetobacter baumannii, allowing its survival on hospital surfaces in the absence of water and nutrients for m...

PhD Project - Next-generation phage therapeutics for combating antibiotic-resistant infections at University of Southampton, listed on FindAPhD.com

Bacteria must distinguish phage attack from normal homeostatic processes, yet the danger signals that trigger many defence systems remain unknown. Here, we show that a PUA-Calcineurin-CE-HAD module from Escherichia coli ECOR28 confers broad anti-phage protection by binding Dam-methylated deoxyadenosine monophosphate (m6-dAMP) generated during phage-induced chromosome degradation. Ligand binding converts a preassembled PUA-Calcineurin-CE hexamer loaded with six HAD phosphatases into a polymerising filament. The filament acts as a high-flux dNTP sink through a two-enzyme cascade: HAD first dephosphorylates dATP to dADP, and Calcineurin-CE then converts dADP to dAMP. dNTP collapse halts phage replication and enforces abortive infection. Multiple mobile-element DNA mimic proteins block filament assembly, revealing a direct phage counter-defence. More broadly, our findings extend a conserved, cross-kingdom paradigm of immune filament assembly to nucleotide-depletion antiviral defence and suggest modified-nucleotide sensing by related PUA-Calcineurin-CE modules as a widespread, underappreciated bacterial strategy. ### Competing Interest Statement The authors have declared no competing interest. NIHR Southampton Biomedical Research Centre, https://ror.org/01qqpzg67, Postdoctoral Bridging Fellowship F.L.N. is supported by a Wessex Health Partners (WHP) and National Institute for Health and Care Research Wessex Experimental Medicine Network (NIHR WEMN), Seed fund National Institutes of Health, GM145888, U24 GM129539) Maloris Foundation Memorial Sloan Kettering Cancer Center, P30-CA008748 Simons Foundation, SF349247 New York State Assembly

Introduction. Chronic wounds are notoriously difficult to treat and are associated with decreased limb function, reduced quality of life and significant morbidity. Their recurrent nature, despite aggr...

AbstractBackground. The global rise of antimicrobial resistance requires innovative and affordable wound care solutions. Moreover, managing wounds infected

Please note that job descriptions are not exhaustive, and you may be asked to take on additional duties that align with the key responsibilities ment...

PhD Project - Advancing urinary tract infection treatment through on-chip technological innovation at University of Southampton, listed on FindAPhD.com

Four world-class teams awarded major BBSRC sLoLa grants to push the frontiers of discovery in microbiology, photosynthesis, gene regulation and quantum biology.

Data also shows an average of nearly 400 newly reported cases of antibiotic-resistant infections a week last year

Bacteria employ a range of competition systems that deliver toxins to inhibit competing strains. This study shows that these systems are particularly important for the ecology of virulent and antibiot...

PhD Project - MRC DiMeN Doctoral Training Partnership: Hiding in plain sight: How multi-drug resistant pathogens evade detection and killing by human neutrophils at Newcastle University, listed on Fin...