Hans Pfalzgraf
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scihans.bsky.social
Hans Pfalzgraf
@scihans.bsky.social
Research Scientist at ProImmune. Biochemistry to help make Ankyron® protein binders. Former crystallographer. I love #SciComm, #Sustainability, #Climbing and #EDM. Views are my own. He/Him.
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November 11, 2025 at 8:15 AM
Reposted by Hans Pfalzgraf
High resolution structural biology approaches and ChemShell QM/MM simulations reveal an unusual structural feature in a cytochrome P460 enzyme, in research published in @chemicalscience.rsc.org

For more details: chemshell.org/mcp460-doubl...
Identifying an unusual structural feature in a cytochrome P460 enzyme - ChemShell
Structural and computational studies indicate the presence of a haem-lysine double crosslink in Methylococcus capsulatus cytochrome P460.
chemshell.org
September 24, 2025 at 1:02 PM
That's one cursed colour scheme for anyone who has learned a little chemistry 😂
September 22, 2025 at 11:33 AM
Reposted by Hans Pfalzgraf
Fascinating insights this #FluorescenceFriday, into complement delay-accelerating factor (DAF, CD55), identified by our Ankyron™ AC40301 (red) in HeLa cells (nuclei, blue) on @discoverecho.bsky.social #microscope.
Find out more about our #animal-free Ankyrons™ at www.proimmune.com/ankyron
May 2, 2025 at 7:22 AM
My protein crystals getting imaged by the Formulatrix Rock Imager. I think it's pretty awesome! 🥰
And convenient to be able to check the images from my desk, including time points to check growth. Beats sitting at a microscope where you can't drink tea! ☕
#Crystallography #ResearchComplexAtHarwell
July 17, 2025 at 5:46 PM
They resealed the electron storage ring of the synchrotron @diamondlightsource.bsky.social during shutdown to get it ready for more experiments!
July 15, 2025 at 11:59 AM
This week I harvested about 450 beautiful protein crystals, more than in my 7 previous years of crystallography! These are crystals of a Covid target in which I soaked promising drug fragments to see what binds and how.
April 17, 2025 at 5:39 PM
I started a new job at @diamondlightsource.bsky.social! A 2nd protein crystallography postdoc, this time for antiviral drug discovery 🤩
It's only funded until October this year, but I'm sure I'll find something else after that 😁
April 16, 2025 at 9:34 AM
Our review is out! It's about β-sheet-based cytochromes, which are involved in the nitrogen cycle. We've now summarized their structures, spectra and ligand-binding. 🧪
doi.org/10.1007/s007...
Cytochromes P460 and c′-β: exploiting a novel fold for multiple functions - JBIC Journal of Biological Inorganic Chemistry
Two related classes of ligand-binding heme c-containing proteins with a high degree of structural homology have been identified and characterized over recent decades: cytochromes P460 (cyts P460), def...
doi.org
March 27, 2025 at 4:21 PM
Hello new followers! 👋
I'm a structural biologist 🧶🧬 currently having fun with unusual kinds of protein crystallography 💎 at Diamond Light Source. 😀
But my 3 years are almost over... 😢
So I'm looking for a lab-based biochemistry job 🧪 in the Oxford area. If you have any leads, please get in touch! 😁
December 5, 2024 at 10:13 AM
Iris in CCP4i2 is very useful to find amino acid residues that are particularly more mobile at room temperature than in cryogenic conditions! #protein #crystallography 🧪
@glycojones.bsky.social I assume that's not the use you had in mind when leading its development? 😁
🧶🧬
November 27, 2024 at 4:06 PM
Making this green protein has been pretty great 🧪 Easy to find it and estimate concentration!
But can you guess WHY it's green? 😋
🧶🧬
November 21, 2024 at 2:51 PM
Blink and you might miss it moving!
The Scorpion is my current favourite instrument. It can make my 96 conditions (2D gradient of concentrations) in half an hour. And look SUPER COOL doing it! 😆
#crystallography 🧪
November 15, 2024 at 12:06 PM
I inherited some dissolved metals from my colleague Peter Smyth and I think they're very pretty 🤩
We use them for long cultures of bacteria, so they don't run out of metal ions when making our favourite proteins 😊
December 19, 2023 at 2:18 PM