Sofía C. Fajardo
@sofiacfajardo.bsky.social
PhD student at University of Bonn.
Molecular Biology for Lysosome Proteomics 🔬📊
Winter Lab, Research Center One Health Ruhr.
Molecular Biology for Lysosome Proteomics 🔬📊
Winter Lab, Research Center One Health Ruhr.
Reposted by Sofía C. Fajardo
Happy to share an exciting study from Yihong Ye’s lab at NIH, with a minor contribution from our lab: ceroid lipofuscinosis-4 (CLN4)-linked DNAJC5 mutations cause lysosomal damage as a driver of neurodegeneration in iPSC-derived neurons. CHIP safeguards lysosomes via microautophagy 👉 rdcu.be/eChof
CHIP protects lysosomes from CLN4 mutant-induced membrane damage
Nature Cell Biology - Lee et al. use an aggregation-prone CLN4 mutant that causes lysosomal damage in neurons and show that in non-neurons, the ubiquitin ligase CHIP prevents CLN4-dependent...
rdcu.be
August 25, 2025 at 1:23 PM
Happy to share an exciting study from Yihong Ye’s lab at NIH, with a minor contribution from our lab: ceroid lipofuscinosis-4 (CLN4)-linked DNAJC5 mutations cause lysosomal damage as a driver of neurodegeneration in iPSC-derived neurons. CHIP safeguards lysosomes via microautophagy 👉 rdcu.be/eChof
Reposted by Sofía C. Fajardo
Ooh nice preprint! Studying membrane nanodomains needs nanoscale lipid localization - current methods fall short. 'Lipid-CLEM', correlative light & EM with click chemistry to map lipids. In endosomes, it shows sphingomyelin’s differential partitioning beautifully.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Visualizing sub-organellar lipid distribution using correlative light and electron microscopy
Lipids and proteins compartmentalize biological membranes into nanoscale domains which are crucial for signaling, intracellular trafficking and many other cellular processes. Studying nanodomain funct...
www.biorxiv.org
January 29, 2025 at 10:52 PM
Ooh nice preprint! Studying membrane nanodomains needs nanoscale lipid localization - current methods fall short. 'Lipid-CLEM', correlative light & EM with click chemistry to map lipids. In endosomes, it shows sphingomyelin’s differential partitioning beautifully.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Sofía C. Fajardo
Don't miss the 2025 Gordon Conference on "Lysosomal Diseases" in California (March 2 - 7, 2025)! t.co/vwcNoDwvHg
December 19, 2024 at 9:11 AM
Don't miss the 2025 Gordon Conference on "Lysosomal Diseases" in California (March 2 - 7, 2025)! t.co/vwcNoDwvHg
Reposted by Sofía C. Fajardo
Hi Bluesky community! 👋
Are you looking forward to 2025 as much as we are? 👀
We just updated our 2025 Annual Poster to include even more events for you to join, so go and have a look!
➡️ s.embl.org/poster-bl
#EMBLEvents #molecularbiology #lifesciencetraining
Are you looking forward to 2025 as much as we are? 👀
We just updated our 2025 Annual Poster to include even more events for you to join, so go and have a look!
➡️ s.embl.org/poster-bl
#EMBLEvents #molecularbiology #lifesciencetraining
December 13, 2024 at 9:06 AM
Hi Bluesky community! 👋
Are you looking forward to 2025 as much as we are? 👀
We just updated our 2025 Annual Poster to include even more events for you to join, so go and have a look!
➡️ s.embl.org/poster-bl
#EMBLEvents #molecularbiology #lifesciencetraining
Are you looking forward to 2025 as much as we are? 👀
We just updated our 2025 Annual Poster to include even more events for you to join, so go and have a look!
➡️ s.embl.org/poster-bl
#EMBLEvents #molecularbiology #lifesciencetraining
Reposted by Sofía C. Fajardo
📢Now up on FocalPlane: Probes 101 – How to choose a fluorescent probe for your imaging experiment.
Lina El Hajji talks us through the optimal photophysical properties & weighs up the pros & cons of FPs versus small molecule dyes & the options in between!
focalplane.biologists.com/2024/12/14/p...
Lina El Hajji talks us through the optimal photophysical properties & weighs up the pros & cons of FPs versus small molecule dyes & the options in between!
focalplane.biologists.com/2024/12/14/p...
Probes 101 – How to choose a fluorescent probe for your imaging experiment - FocalPlane
Probes 101 – How to choose a fluorescent probe for your imaging experiment - Tools
focalplane.biologists.com
December 16, 2024 at 11:46 AM
📢Now up on FocalPlane: Probes 101 – How to choose a fluorescent probe for your imaging experiment.
Lina El Hajji talks us through the optimal photophysical properties & weighs up the pros & cons of FPs versus small molecule dyes & the options in between!
focalplane.biologists.com/2024/12/14/p...
Lina El Hajji talks us through the optimal photophysical properties & weighs up the pros & cons of FPs versus small molecule dyes & the options in between!
focalplane.biologists.com/2024/12/14/p...
Great lecture from Prof. Neefjes in his visit to the Winter Lab!
November 29, 2024 at 5:11 PM
Great lecture from Prof. Neefjes in his visit to the Winter Lab!
Reposted by Sofía C. Fajardo
I was looking for a starter pack for #WomenInSTEM working on #CellBiology and didn't find any - so I created one. And since I am hoping to not misgender anyone I made it #FLINTA (fingers crossed). Let me know if you want to be added!
go.bsky.app/5fp61o6
go.bsky.app/5fp61o6
November 20, 2024 at 11:03 AM
I was looking for a starter pack for #WomenInSTEM working on #CellBiology and didn't find any - so I created one. And since I am hoping to not misgender anyone I made it #FLINTA (fingers crossed). Let me know if you want to be added!
go.bsky.app/5fp61o6
go.bsky.app/5fp61o6
Reposted by Sofía C. Fajardo
Are you an #ECR who would like to attend a #microscopy or #bioimageanalysis training course in the next ~6 months but don't have sufficient funding, check to see if you are eligible for our JCS-FocalPlane Training Grant.
Next deadline: 22 November 2024
www.biologists.com/grants/jcs-f...
Next deadline: 22 November 2024
www.biologists.com/grants/jcs-f...
November 19, 2024 at 2:02 PM
Are you an #ECR who would like to attend a #microscopy or #bioimageanalysis training course in the next ~6 months but don't have sufficient funding, check to see if you are eligible for our JCS-FocalPlane Training Grant.
Next deadline: 22 November 2024
www.biologists.com/grants/jcs-f...
Next deadline: 22 November 2024
www.biologists.com/grants/jcs-f...
Reposted by Sofía C. Fajardo
Q: What's wrong with these plots?
A: Pooling cell-level measurements across multiple runs conceals experimental reproducibility.
Read more in JCB (with @fritzlaylin.bsky.social @katrinavelle.bsky.social & Dyche Mullins): rupress.org/jcb/article/...
A: Pooling cell-level measurements across multiple runs conceals experimental reproducibility.
Read more in JCB (with @fritzlaylin.bsky.social @katrinavelle.bsky.social & Dyche Mullins): rupress.org/jcb/article/...
November 18, 2024 at 7:02 PM
Q: What's wrong with these plots?
A: Pooling cell-level measurements across multiple runs conceals experimental reproducibility.
Read more in JCB (with @fritzlaylin.bsky.social @katrinavelle.bsky.social & Dyche Mullins): rupress.org/jcb/article/...
A: Pooling cell-level measurements across multiple runs conceals experimental reproducibility.
Read more in JCB (with @fritzlaylin.bsky.social @katrinavelle.bsky.social & Dyche Mullins): rupress.org/jcb/article/...
Reposted by Sofía C. Fajardo
Reposted by Sofía C. Fajardo
Here are some free/open-source sites for the preparation of scientific illustrations, including icon libraries: bioicons.com, scidraw.io, bioart.niaid.nih.gov, inkscape.org, biogdp.com, reactome.org/icon-lib
November 17, 2024 at 4:04 PM
Here are some free/open-source sites for the preparation of scientific illustrations, including icon libraries: bioicons.com, scidraw.io, bioart.niaid.nih.gov, inkscape.org, biogdp.com, reactome.org/icon-lib
Barely started on Twitter but seems like the real fun is here!
November 18, 2024 at 8:25 PM
Barely started on Twitter but seems like the real fun is here!