The Freeman Lab
@spencerfreeman.bsky.social
We’ve engineered stable cell systems to induce lipid scrambling of the plasma membrane with active forms of TMEM16F. Scrambling alone causes vesiculation (🔵TMEM16F-active; 🟠PS/Annexin V). Let us know if you want to try the cells out. Preprint: www.biorxiv.org/content/10.1...
May 14, 2025 at 10:46 PM
We’ve engineered stable cell systems to induce lipid scrambling of the plasma membrane with active forms of TMEM16F. Scrambling alone causes vesiculation (🔵TMEM16F-active; 🟠PS/Annexin V). Let us know if you want to try the cells out. Preprint: www.biorxiv.org/content/10.1...
Reposted by The Freeman Lab
Sorting nexin 10 regulates lysosomal ionic homeostasis via ClC-7 by controlling PI(3,5)P2, say Jing Ze Wu, Sergio Grinstein, Spencer Freeman and colleagues @sickkidsto.bsky.social: rupress.org/jcb/article/...
#Immunology #Membrane #lipid #lysosomes #osteopetrosis
#Immunology #Membrane #lipid #lysosomes #osteopetrosis
April 2, 2025 at 6:17 PM
Sorting nexin 10 regulates lysosomal ionic homeostasis via ClC-7 by controlling PI(3,5)P2, say Jing Ze Wu, Sergio Grinstein, Spencer Freeman and colleagues @sickkidsto.bsky.social: rupress.org/jcb/article/...
#Immunology #Membrane #lipid #lysosomes #osteopetrosis
#Immunology #Membrane #lipid #lysosomes #osteopetrosis
Reposted by The Freeman Lab
This work was done in tandem with cool work from the @spencerfreeman.bsky.social lab identifying novel regulators of PI3,5P2 in lysosomes
rupress.org/jcb/article-...
rupress.org/jcb/article-...
Sorting nexin 10 regulates lysosomal ionic homeostasis via ClC-7 by controlling PI(3,5)P2
Snx10 was found to determine the level of PI(3,5)P2 in (phago)lysosomes by controlling the supply of PI(3)P from upstream endocytic compartments. By regula
rupress.org
March 31, 2025 at 4:25 PM
This work was done in tandem with cool work from the @spencerfreeman.bsky.social lab identifying novel regulators of PI3,5P2 in lysosomes
rupress.org/jcb/article-...
rupress.org/jcb/article-...
Reposted by The Freeman Lab
Pemberton, Burke, Balla et al. report the generation & characterization of an engineered active fragment of PIKfyve and its use to generate extra PI(3,5)P2 in endomembrane compartments & test the function of PI(3,5)P2 #biosensors rupress.org/jcb/article/...
#lipids #CellSignaling
#lipids #CellSignaling
March 26, 2025 at 7:17 PM
Pemberton, Burke, Balla et al. report the generation & characterization of an engineered active fragment of PIKfyve and its use to generate extra PI(3,5)P2 in endomembrane compartments & test the function of PI(3,5)P2 #biosensors rupress.org/jcb/article/...
#lipids #CellSignaling
#lipids #CellSignaling
New tools and regulators of lysosomal PI(3,5)P2!
Lipidomics by Sin, Junko, Takehiko (Tokyo) and a new toolkit for PIKfyve/PI(3,5)P2 by Josh and Tamas (NIH). Check out JIm's paper here: hubs.la/Q03dym0b0 or: shorturl.at/yq9K8 and the paper by Balla, Burke, Pemberton et al. here: hubs.la/Q03dyfL60
Lipidomics by Sin, Junko, Takehiko (Tokyo) and a new toolkit for PIKfyve/PI(3,5)P2 by Josh and Tamas (NIH). Check out JIm's paper here: hubs.la/Q03dym0b0 or: shorturl.at/yq9K8 and the paper by Balla, Burke, Pemberton et al. here: hubs.la/Q03dyfL60
March 27, 2025 at 5:27 PM
New tools and regulators of lysosomal PI(3,5)P2!
Lipidomics by Sin, Junko, Takehiko (Tokyo) and a new toolkit for PIKfyve/PI(3,5)P2 by Josh and Tamas (NIH). Check out JIm's paper here: hubs.la/Q03dym0b0 or: shorturl.at/yq9K8 and the paper by Balla, Burke, Pemberton et al. here: hubs.la/Q03dyfL60
Lipidomics by Sin, Junko, Takehiko (Tokyo) and a new toolkit for PIKfyve/PI(3,5)P2 by Josh and Tamas (NIH). Check out JIm's paper here: hubs.la/Q03dym0b0 or: shorturl.at/yq9K8 and the paper by Balla, Burke, Pemberton et al. here: hubs.la/Q03dyfL60
Reposted by The Freeman Lab
Excited to post for the first time to present our exciting new paper @Nature. The fruit of massive work by Tommaso Vicanolo, @alazozcan.bsky.social and Jackson Li, and our superb collaborators Laiguan and Stefan Uderhardt. True teamwork!
Free link: rdcu.be/eeeA2
www.nature.com/articles/s41...
Free link: rdcu.be/eeeA2
www.nature.com/articles/s41...
Matrix-producing neutrophils populate and shield the skin
Nature - A population of neutrophils in the skin produces extracellular matrix, providing a defence strategy by reinforcing the barrier properties of the skin and helping to block the entry of...
rdcu.be
March 19, 2025 at 9:28 PM
Excited to post for the first time to present our exciting new paper @Nature. The fruit of massive work by Tommaso Vicanolo, @alazozcan.bsky.social and Jackson Li, and our superb collaborators Laiguan and Stefan Uderhardt. True teamwork!
Free link: rdcu.be/eeeA2
www.nature.com/articles/s41...
Free link: rdcu.be/eeeA2
www.nature.com/articles/s41...
Simultaneous CRISPR screening and spatial transcriptomics reveal intracellular, intercellular, and functional transcriptional circuits: Cell www.cell.com/cell/fulltex...
Simultaneous CRISPR screening and spatial transcriptomics reveal intracellular, intercellular, and functional transcriptional circuits
Perturb-FISH, a method that combines high-throughput imaging spatial transcriptomics
with in situ guide RNA detection, allows for the analysis of molecular and functional
states, with spatial resoluti...
www.cell.com
March 12, 2025 at 10:04 PM
Simultaneous CRISPR screening and spatial transcriptomics reveal intracellular, intercellular, and functional transcriptional circuits: Cell www.cell.com/cell/fulltex...
We've been studying mechanisms of resilience that protect lysosomes from damage and wrote a review on the topic. We consider lysosomal stress and damage on a spectrum. Please help me share as this is my first post 😀 and I don't have many followers! 🙏 Open Access: www.embopress.org/doi/full/10....
February 27, 2025 at 3:54 PM
We've been studying mechanisms of resilience that protect lysosomes from damage and wrote a review on the topic. We consider lysosomal stress and damage on a spectrum. Please help me share as this is my first post 😀 and I don't have many followers! 🙏 Open Access: www.embopress.org/doi/full/10....