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The Freeman Lab @spencerfreeman.bsky.social · May 14

We’ve engineered stable cell systems to induce lipid scrambling of the plasma membrane with active forms of TMEM16F. Scrambling alone causes vesiculation (🔵TMEM16F-active; 🟠PS/Annexin V). Let us know if you want to try the cells out. Preprint: www.biorxiv.org/content/10.1...

HeLa cells stably expressing a dox-inducible active TMEM16F-GFP (blue). Once expressed, lipid scrambling of the plasma membrane bilayer causes vesiculation, shown with Annexin V staining of the exposed PS (gold).

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Reposted by The Freeman Lab

Journal of Cell Biology @jcb.org · Apr 2

Sorting nexin 10 regulates lysosomal ionic homeostasis via ClC-7 by controlling PI(3,5)P2, say Jing Ze Wu, Sergio Grinstein, Spencer Freeman and colleagues @sickkidsto.bsky.social: rupress.org/jcb/article/...

#Immunology #Membrane #lipid #lysosomes #osteopetrosis

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Reposted by The Freeman Lab

John Burke @jburkevic.bsky.social · Mar 31

This work was done in tandem with cool work from the @spencerfreeman.bsky.social lab identifying novel regulators of PI3,5P2 in lysosomes
rupress.org/jcb/article-...

Sorting nexin 10 regulates lysosomal ionic homeostasis via ClC-7 by controlling PI(3,5)P2

Snx10 was found to determine the level of PI(3,5)P2 in (phago)lysosomes by controlling the supply of PI(3)P from upstream endocytic compartments. By regula

rupress.org

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Reposted by The Freeman Lab

Journal of Cell Biology @jcb.org · Mar 26

Pemberton, Burke, Balla et al. report the generation & characterization of an engineered active fragment of PIKfyve and its use to generate extra PI(3,5)P2 in endomembrane compartments & test the function of PI(3,5)P2 #biosensors rupress.org/jcb/article/...

#lipids #CellSignaling

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The Freeman Lab @spencerfreeman.bsky.social · Mar 27

New tools and regulators of lysosomal PI(3,5)P2!
Lipidomics by Sin, Junko, Takehiko (Tokyo) and a new toolkit for PIKfyve/PI(3,5)P2 by Josh and Tamas (NIH). Check out JIm's paper here: hubs.la/Q03dym0b0 or: shorturl.at/yq9K8 and the paper by Balla, Burke, Pemberton et al. here: hubs.la/Q03dyfL60

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Reposted by The Freeman Lab

Andres Hidalgo @drusqui.bsky.social · Mar 19

Excited to post for the first time to present our exciting new paper @Nature. The fruit of massive work by Tommaso Vicanolo, @alazozcan.bsky.social and Jackson Li, and our superb collaborators Laiguan and Stefan Uderhardt. True teamwork!
Free link: rdcu.be/eeeA2
www.nature.com/articles/s41...

Matrix-producing neutrophils populate and shield the skin

Nature - A population of neutrophils in the skin produces extracellular matrix, providing a defence strategy by reinforcing the barrier properties of the skin and helping to block the entry of...

rdcu.be

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The Freeman Lab @spencerfreeman.bsky.social · Mar 12

Simultaneous CRISPR screening and spatial transcriptomics reveal intracellular, intercellular, and functional transcriptional circuits: Cell www.cell.com/cell/fulltex...

Simultaneous CRISPR screening and spatial transcriptomics reveal intracellular, intercellular, and functional transcriptional circuits

Perturb-FISH, a method that combines high-throughput imaging spatial transcriptomics with in situ guide RNA detection, allows for the analysis of molecular and functional states, with spatial resoluti...

www.cell.com

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The Freeman Lab @spencerfreeman.bsky.social · Feb 27

We've been studying mechanisms of resilience that protect lysosomes from damage and wrote a review on the topic. We consider lysosomal stress and damage on a spectrum. Please help me share as this is my first post 😀 and I don't have many followers! 🙏 Open Access: www.embopress.org/doi/full/10....

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