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John Burke @jburkevic.bsky.social · 28d

This was part of a special issue to celebrate 50 years since the landmark review from Bob Michell on phosphoinositide signalling. Looking forward to reading the full cohort of papers from this issue

John Burke @jburkevic.bsky.social · 28d

New review out from my lab on class I PI3Ks www.sciencedirect.com/science/arti..., was fun to write where we are and where we are going in PI3K research
Great work from Isobel and Alex in my lab @alexlouiseshaw.bsky.social

Molecular basis for regulation of the class I phosphoinositide 3-kinases (PI3Ks), and their targeting in human disease

The class I phosphoinositide 3-kinase pathway (PI3K) is a master regulator of cellular growth, and plays essential roles in controlling immune cell fu…

www.sciencedirect.com

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Reposted by John Burke

Gerry Hammond @gerryhammond.bsky.social · 28d

Announcing our new PA biosensor, PILS-Nir1 – seen here in HeLa cells during PLC activation #lipidtime #phospholipids

Read the OA paper in JCB

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John Burke @jburkevic.bsky.social · Sep 6

Congrats James

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Reposted by John Burke

Gerry Hammond @gerryhammond.bsky.social · Aug 22

How do swelling lysosomes not burst? Because they literally suck more lipids into their membranes from the ER via this protein straw. Amazing piece of this particular molecular puzzle from my colleague jay tab!

Cell Biology J-Club @cellclub.bsky.social · Aug 21

LYVAC/PDZD8 is a lysosomal vacuolator | Science www.science.org/doi/10.1126/...

LYVAC/PDZD8 is a lysosomal vacuolator

Lysosomal vacuolation is commonly found in many pathophysiological conditions, but its molecular mechanisms and functions remain largely unknown. Here, we show that the endoplasmic reticulum (ER)–anch...

www.science.org

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Reposted by John Burke

tafesselab.bsky.social @tafesselab.bsky.social · Aug 12

Have you published lipid-protein interactome data? Please let us know, we’d love to include it in the repository. The goal is to build a centralized hub for the scientific community.

Huge thanks to Gaelen Guzman, a graduate student/postdoc in the lab who built it from scratch.

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Reposted by John Burke

Alexandria Shaw @alexlouiseshaw.bsky.social · Jul 7

New review out with @jburkevic.bsky.social!
We highlight recent advances in our understanding of the role the phosphoinositide PIP3 plays in regulating the protein kinases Akt, PDK1, and BTK from a structural perspective.
#kinase #phosphoinositides

portlandpress.com/biochemsoctr...

Molecular insight on the role of the phosphoinositide PIP3 in regulating the protein kinases Akt, PDK1, and BTK

Protein kinases are master regulators of myriad processes in eukaryotic cells playing critical roles in growth, metabolism, cellular differentiation, and motility. A subclass of protein kinases is reg...

portlandpress.com

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Reposted by John Burke

tafesselab.bsky.social @tafesselab.bsky.social · Jun 18

Excited to share our new preprint 🥳. We dev’ed a high-affinity nanobody that binds MR1 & blocks MR1-restricted T cell activation, a powerful tool to dissect MR1 biology in infection, inflammation & cancer. @jburkevic.bsky.social @ohsunews.bsky.social

www.biorxiv.org/content/10.1...

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Reposted by John Burke

Peter Mace @petermace.bsky.social · Jun 17

Check out the view today from the venue—scoping for the IUBMB Focussed meeting on (pseudo)kinases! Abstracts and early bird registrations closes this week!

www.ivvy.com.au/event/IUBMB25/

@pseudokinase.bsky.social @iubmb.bsky.social

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Reposted by John Burke

Peter Mace @petermace.bsky.social · Jun 12

Only one week left to submit your abstract and register at early bird rates for the IUBMB Focussed Meeting - (pseudo)kinases in signal transduction. Sign up and register by June 20!! Join @pseudokinase.bsky.social Natalia Jura, and a who's who of kinase research!

www.ivvy.com.au/event/IUBMB25/

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Reposted by John Burke

Gerry Hammond @gerryhammond.bsky.social · Apr 12

We have some #lipids talks at #asbmb25 on PI signaling that will blow your mind! Join us Sunday at 9 a.m. in room W179

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Reposted by John Burke

Carolyn Bertozzi @carolynbertozzi.bskyverified.social · Apr 11

SCAM ALERT. This is a fake conference that ripped off my and other people’s names to trick people into registering. There is no such conference that I or the others shown were invited or agreed to. We are working to try to get this fraud taken down from the web.

chemsymposia.com

Join Chemsymposia 2026: Research & Innovation | ChemSymposia 2026

Join us at Chemsymposia 2026 to discover groundbreaking research in physics applications. Connect with experts, engage in insightful discussions, and explore our venue, registration details, and speak...

chemsymposia.com

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John Burke @jburkevic.bsky.social · Apr 3

A really exciting new preprint out from the Jura and Verba labs looking at conformational dynamics of PI3K on membrane surfaces using cryo-EM. This is truly groundbreaking, and provides a ton of exciting insight into PI3K activation
www.biorxiv.org/content/10.1...

Structures of the PI3Kα/KRas complex on lipid bilayers reveal the molecular mechanism of PI3Kα activation

PI3Kα is a potent oncogene that converts PIP2 to PIP3 at the plasma membrane upon activation by receptor tyrosine kinases and Ras GTPases. In the absence of any structures of activated PI3Kα, the mole...

www.biorxiv.org

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John Burke @jburkevic.bsky.social · Mar 31

This work was done in tandem with cool work from the @spencerfreeman.bsky.social lab identifying novel regulators of PI3,5P2 in lysosomes
rupress.org/jcb/article-...

Sorting nexin 10 regulates lysosomal ionic homeostasis via ClC-7 by controlling PI(3,5)P2

Snx10 was found to determine the level of PI(3,5)P2 in (phago)lysosomes by controlling the supply of PI(3)P from upstream endocytic compartments. By regula

rupress.org

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John Burke @jburkevic.bsky.social · Mar 31

New work out from my lab by my graduate student Isobel in collaboration with Josh Pemberton and Tamas Balla. Provides some new cool tools to manipulate PI3,5P2 levels, as well as some new insight into PIKfyve regulation.
rupress.org/jcb/article-...

An advanced toolset to manipulate and monitor subcellular phosphatidylinositol 3,5-bisphosphate

In this paper, Pemberton et al. describe the generation and characterization of an engineered active fragment of the PIKfyve enzyme and its use to generate

rupress.org

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John Burke @jburkevic.bsky.social · Mar 5

Thanks Fikadu, so cool to see everything you and your lab are up to. Best seminar of the year so far!

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Reposted by John Burke

Peter Mace @petermace.bsky.social · Feb 26

Really exciting to share the peer-reviewed version of our study of the DET1 adaptor for the CUL4 Ub ligase. A cryptic E2-binding site and recruitment mechanism for COP1—another E3!

www.science.org/doi/10.1126/...

DET1 dynamics underlie cooperative ubiquitination by CRL4DET1-COP1 complexes

A dynamic substrate adapter (DET1) recruits COP1 to Cullin ubiquitin ligase complexes assisted by a noncatalytic E2 enzyme.

www.science.org

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Reposted by John Burke

Simon Cook @drsimoncook.bsky.social · Feb 12

I'm looking forward to this Signalling Symposium which celebrates the achievements of Len Stephens and Phill Hawkins during their 30-yr tenure at the Babraham Institute @babrahaminst.bsky.social
Registration details below

Babraham Institute @babrahaminst.bsky.social · Feb 12

📢 Calling all Signallers! Join us for this celebratory smorgasbord of signalling research on 1-2 May with sessions on #proteostasis, #PI3K, #autophagy, #redox biology and #nuclear signalling. 🧪

Latest Institute research with special guests ⤵️

www.eventbrite.co.uk/e/cellular-s...

Cellular signals for health & disease

A symposium marking the contributions of Len Stephens and Phillip Hawkins

www.eventbrite.co.uk

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Reposted by John Burke

Gerry Hammond @gerryhammond.bsky.social · Feb 11

The most frequently asked question we get: do #lipidtime genetically encoded lipid biosensors compete out cells’ native effector proteins?

Victoria Holmes and Morgan Ricci led a paper in JCB that answers this question:

rupress.org/jcb/article/...

Read on for a brief thread.

Single-molecule lipid biosensors mitigate inhibition of endogenous effector proteins | Journal of Cell Biology | Rockefeller University Press

Holmes and colleagues show that AKT activation is blocked by commonly used PIP3 biosensors, but that this can be overcome by expressing biosensors at singl

rupress.org

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Reposted by John Burke

Peter Mace @petermace.bsky.social · Jan 29

Exciting to open registration for the IUBMB Focussed Meeting on Emerging roles of (pseudo)kinases! Fantastic speakers in an unmatched location, lots of speaking slots available for ECRS.
Interested in kinases or pseudokinases? We would love to see you in Queenstown!

www.ivvy.com.au/event/IUBMB2...

Poster showing a kinase domain emerging from Lake Wakatipu, with a list of speakers

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John Burke @jburkevic.bsky.social · Dec 21

I think this might be my nominee for coolest paper I have seen all year (coming in at the buzzer)

John Burke @jburkevic.bsky.social · Dec 21

new paper using an optopharmacology approach to target the opioid receptor gpcr out in biorxiv today. This will be a perfect addition to the teaching of the gpcr section of my 4th year course. www.biorxiv.org/content/10.1...
Fun fact I worked in Dirk Trauner’s lab during my senior year at Berkeley

An opioid efficacy switch for reversible optical control of peripheral analgesia

The mu-opioid receptor (MOR) is a major target for the treatment of pain. However, opioids are prone to side effects which limit their effectiveness as analgesics and can lead to opioid use disorders ...

www.biorxiv.org

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John Burke @jburkevic.bsky.social · Dec 20

Josh Pemberton and Tamas Balla were able to do some very cool in cell BRET assays to validate this interface. Overall, this work all together provides some new insight into how PI4P production at the PM is regulated.

John Burke @jburkevic.bsky.social · Dec 20

Alex and Sushant reconstituted this tetrameric complex and solved its structure by cryo EM. Intriguingly, EFR3 forms a V shaped interface with both the TTC7-FAM126 subunits. Lots of PTMs of previously unannotated function map to this site.

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