🚨 New preprint out!
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.

www.biorxiv.org/content/10.1...

Thank you for the highlight! 🙌

Can’t wait for the BioMatter Meeting in beautiful Arolla 🇨🇭⛰️
Amazing lineup of speakers + cutting-edge science on cytoskeleton dynamics from cells to tissues.
See you there!

(5/5)
Impaired adhesion is driven by changes in tubulin levels and microtubule stability.
Reducing tubulin (T007-1) or mild destabilization (nocodazole) rescues the phenotype, while hyperstabilization (taxol) worsens it.
📌Tubulin homeostasis supports multicellular organization.

(4/5)
How?
Loss of autoregulation disrupts localization of adhesion molecules like N-Cadherin, Fibronectin, and Integrin α5: cells round up and lose anchorage.
📌Restoring adhesion (via E-Cadherin over expression or ECM) rescues tissue integrity and viability.

(3/5)
Tubulin autoregulation is essential for spheroid architecture & viability. Without it:
- cells detach (corona forms)
- cell death increases
📌This phenotype isn’t due to cell cycle defects or migration — it reflects impaired cell adhesion.

(2/5)
Loss or mutation of TTC5, the key mediator of tubulin autoregulation, increases tubulin mRNA and protein levels in 3D spheroids. This leads to microtubule hyperstability and reorganization of the network.
📌Tubulin levels matter (even in steady state!)

🔎New preprint!
We reveal a physiological role for tubulin autoregulation, fine-tuning microtubule dynamics and preserving 3D cellular architecture.
Big thanks to @ivanagasic.bsky.social and team!
Link below for all #cytoskeleton & #spheroid enthusiasts👇(1/5)
www.biorxiv.org/content/10.1...

(5/5)
Impaired adhesion is driven by changes in tubulin levels and microtubule stability.
Reducing tubulin (T007-1) or mild destabilization (nocodazole) rescues the phenotype, while hyperstabilization (taxol) worsens it.
📌Tubulin homeostasis supports multicellular organization.

(4/5)
How?
Loss of autoregulation disrupts localization of adhesion molecules like N-Cadherin, Fibronectin, and Integrin α5: cells round up and lose anchorage.
📌Restoring adhesion (via E-Cadherin over expression or ECM) rescues tissue integrity and viability.

(3/5)
Tubulin autoregulation is essential for spheroid architecture & viability. Without it:
- cells detach (corona forms)
- cell death increases
📌This phenotype isn’t due to cell cycle defects or migration — it reflects impaired cell adhesion.

2/5)
Loss or mutation of TTC5, the key mediator of tubulin autoregulation, increases tubulin mRNA and protein levels in 3D spheroids. This leads to microtubule hyperstability and reorganization of the network.
📌Tubulin levels matter (even in steady state!)

EMBO Fellows Meeting 2025 just wrapped up!
4 intense days, 110+ talks, amazing postdocs, mentoring & networking!
Huge thanks to @embo.org & the organizers 👏
Feeling proud to be part of the next gen of scientists ready to push science forward.
Sharing a few highlights 📸

📢We are live everyone ! ! !

We are happy to announce that our official WEBSITE is now online - and early bird registration is OPEN 🎉

👀👇Come check it out🚀🌟

spaom2025.org
🚩Braga, Portugal
📅November 5-7, 2025

#microscopy #science #bioimaging #advancedmicroscopy #spatialbiology #photonics

Last call for abstracts! 👇
#postdocday

Two cells, one twist 🧬🔁
Myosin asymmetry breaks mirror symmetry & sparks spontaneous 3D rotations in doublets.
Adherens junctions🩶, focal adhesions🟢 & actomyosin🔴 Biology meets active matter physics.
#CellPolarity #Fluorescencefriday #ActiveMatter #Microscopy

Did you know the cell wall of mycobacteria isn’t just for protection? 🧱👀 Here, Mycobacterium marinum sheds its outer capsule right after being eaten by Dicty! A sneaky move that affects even nearby cells… #FluorescenceFriday #Mycobacteria #CellBiology #Infection #3Rmodels #Phagocytosis

New lab preprint! Unveiling the Molecular Architecture of T Cells and Immune Synapses with Cryo-Expansion Microscopy (Cryo-ExM). A fantastic collaboration with Dr. Benita Wolf. Congratulations to all authors, especially Florent Lemaitre, for leading this amazing work! www.biorxiv.org/content/10.1...

🔬 A new probe developed by Janelia researchers & collaborators that enables visualization of extracellular matrix structure in live tissues could also be useful in studying diseases linked to changes in the extracellular matrix and in diagnostic imaging ➡️ www.janelia.org/news/looking...

This stunning image was made by @ac-almeida.bsky.social , our brilliant President and microtubule enthusiast! She's passionate about how the cytoskeleton organizes itself in 3D—seriously cool stuff! 🧠🧵✨#Microscopy #WomenInSTEM #Cytoskeleton
@mocel.bsky.social

Behold: a human cancer spheroid! This 3D cell blob models solid tumors. DAPI (blue) marks nuclei, PI lights up the dead. Z-plane colors unveil a rainbow necrotic core wrapped in a moody, monochrome outer layer. #fluorescencefriday #microscopy #postdoc