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Reposted by Alfred Millett-Sikking

Torsten Wittmann @microtubule.bsky.social · 10h

Playing around with how to represent volume in #Snouty light sheet data. Here is a dividing hiPSC with endogenously tagged histone in all three orthogonal projections. Bluer is further away. If anything, it distracts from politics.

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Reposted by Alfred Millett-Sikking

Reto Fiolka @retof.bsky.social · 2d

We present multi-immersion Oblique Plane microscope (miOPM), a light-sheet platform that can be adapted to a wide range of applications, from sensitive live cell imaging to imaging organs and cleared tissues.
www.biorxiv.org/content/10.1...

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Alfred Millett-Sikking @amsikking.bsky.social · 9d

Done! 👍

Alfred Millett-Sikking @amsikking.bsky.social · 10d

The culmination of many years work. One of the most advanced light-sheet microscopes in the world!

-> A large field of view Snouty light-sheet microscope, with any immersion remote refocus and real-time multi-angle projections!

For details see previous posts and here: github.com/amsikking/HT...

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Alfred Millett-Sikking @amsikking.bsky.social · 10d

Celebrating science with art! Every 2 weeks for 1 year, the full alphabet. Like, reply or repost with your favorite "P" scientist!

(16/26) -> "P" is for Pelican, Pineapple, and...Planck?

en.wikipedia.org/wiki/Max_Pla...

Art by @annesikking.bsky.social (my Mum).

Alfred Millett-Sikking @amsikking.bsky.social · 21d

Queen? ;)

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Reposted by Alfred Millett-Sikking

Kari Herrington @kahsage.bsky.social · 21d

@tanner-fadero.bsky.social told me there was a discussion about the unofficial name for #snouty V3. We have had Mr. Snouty and King Snouty, and there were people suggesting Emperor Snouty. I think it is time for a Lady or EMPRESS snouty. I have created Art to support this.

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Alfred Millett-Sikking @amsikking.bsky.social · 24d

This is a great addition to any microscope that uses remote refocus optics (like a Snouty light-sheet microscope).

-> It makes for a very versatile 3D imaging platform with minimal impact on transmission efficiency.

For more details see the publication here:
amsikking.github.io/any_immersio...

Any_immersion_remote_refocus_microscopy_by_AMSikking

amsikking.github.io

Alfred Millett-Sikking @amsikking.bsky.social · 24d

So whereas normally we arrange remote refocus optics as a series of static lenses that are optimized for a particular sample RI (e.g. 1.51 for oily samples).

-> Here we show a zoom lens design that can dynamically adjust to any sample refractive index from water to oil! (i.e. RI range 1.33-1.51)

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Alfred Millett-Sikking @amsikking.bsky.social · 24d

What if we could get good 3D imaging in 'any' sample refractive index? Previously we showed how remote refocus optics can get good 3D imaging in a 'watery' sample (RI=1.33) with any immersion objective.

-> Here we show how a zoom lens can adjust remote refocus optics to any sample refractive index!

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Alfred Millett-Sikking @amsikking.bsky.social · 24d

Celebrating science with art! Every 2 weeks for 1 year, the full alphabet. Like, reply or repost with your favorite "O" scientist!

(15/26) -> "O" is for Octopus, Orange, and...Ohm?

en.wikipedia.org/wiki/Georg_Ohm

Art by @annesikking.bsky.social (my Mum).

Reposted by Alfred Millett-Sikking

Kevin Dean @kevin-dean.bsky.social · Sep 2

Thrilled to share our new work in Cell Reports Methods: MCT-ASLM, a multiscale light-sheet microscopy platform that captures biology from tissue landscapes down to organelles, revealing rare events like sparse neurons and micrometastases. www.cell.com/cell-reports...

Feature-driven whole-tissue imaging with subcellular resolution

Lin et al. present a self-driving light-sheet microscope that integrates whole-organ surveys with automated, high-resolution targeting of either user-specified or autonomously identified features. By ...

www.cell.com

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Alfred Millett-Sikking @amsikking.bsky.social · Sep 1

I called this technique 'any immersion remote refocus' (AIRR) microscopy.

-> For more details see the publication here: amsikking.github.io/any_immersio...

Any_immersion_remote_refocus_microscopy_by_AMSikking

amsikking.github.io

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Alfred Millett-Sikking @amsikking.bsky.social · Sep 1

In practice, using an air objective to image deep into a liquid sample is a powerful technique!

-> The air objective is easy to use and automate, and doesn't fail like liquid immersion (i.e. not sufficiently hydrated, bubbles, etc). It's also thermally isolated and allows high speed tilling!

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Alfred Millett-Sikking @amsikking.bsky.social · Sep 1

The different immersion options have different advantages.

-> Here I show that (with a watery sample) the water objective can image the deepest, the oil objective has the highest numerical aperture and the air objective avoids liquid immersion!

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Alfred Millett-Sikking @amsikking.bsky.social · Sep 1

This enables new and interesting imaging regimes with totally mismatched immersion and sample refractive index!

-> Here I show a remote refocus microscope imaging deep into a watery sample. The water immersion works well (as expected), but so does the air and oil immersion!

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Alfred Millett-Sikking @amsikking.bsky.social · Sep 1

What if we could use 'any' immersion objective and get good 3D imaging? Normally we can't as we must refractive index match the immersion to the sample (previous post).

-> However, I realized a few years ago that we can get good 3D imaging with any immersion using remote refocus optics!

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Alfred Millett-Sikking @amsikking.bsky.social · Sep 1

Celebrating science with art! Every 2 weeks for 1 year, the full alphabet. Like, reply or repost with your favorite "N" scientist!

(14/26) -> "N" is for Narwhal, Nasturtium, and...Newton?

en.wikipedia.org/wiki/Isaac_N...

Art by @annesikking.bsky.social (my Mum).

Alfred Millett-Sikking @amsikking.bsky.social · Sep 1

👍

Reposted by Alfred Millett-Sikking

Torsten Wittmann @microtubule.bsky.social · Aug 28

Some first raw (not deskewed) data of live human induced pluripotent stem cells from #Snouty now ready for some serious live imaging. Light sheet moving at a 38-degree angle through the cell layer; coverslip is on the bottom.

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Reposted by Alfred Millett-Sikking

BWJones @bwjones.bsky.social · Aug 27

Bluesky is now the platform of choice for the scientific community.

From @jenlucpiquant.bsky.social on @arstechnica.com

arstechnica.com/science/2025...

Bluesky now platform of choice for science community

It’s not just you. Survey says: “Twitter sucks now and all the cool kids are moving to Bluesky”…

arstechnica.com

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Reposted by Alfred Millett-Sikking

Amir Rahmani @astormic.bsky.social · Aug 27

👀 What if your flow cytometer could see the invisible?

Our new preprint introduces single-molecule flow cytometry (smFC), pushing detection sensitivity 10–80× beyond conventional flow cytometry.
www.biorxiv.org/content/10.1...

Single-molecule flow cytometry

Flow cytometry (FC) is a powerful tool for high-throughput characterisation of cell populations, yet its ensemble fluorescence detection and the autofluorescence of cells impose a sensitivity limit of...

www.biorxiv.org

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Alfred Millett-Sikking @amsikking.bsky.social · Aug 25

Yes, the appropriate correction collar can indeed correct an image with RI mismatch. Although, for 3D imaging (i.e. Z stacks) this approach is often slow/awkward and only fully corrects at 1 location in Z. It's good to have some responses so thanks for engaging 👍

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Alfred Millett-Sikking @amsikking.bsky.social · Aug 25

So this is why immersion objectives exist! I.e. water immersion, silicone oil immersion, oil immersion etc. Not just for improving resolution but for enabling 3D imaging!

-> In practice we can't always RI match the immersion to the sample and so sometimes we just get blurry 3D data!

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Alfred Millett-Sikking @amsikking.bsky.social · Aug 25

In practice it looks something like this...

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