Job Details: The IRACDA Program at the University of Kansas (KU) is accepting applications for postdoctoral scholarships. W

Protein tagging with CRISPR-Cas9 enables the investigation of protein function in its native environment but is limited by low homology-directed repair (HDR) efficiency. Here, we present a protocol for fast antibiotic resistance-based gene editing with CRISPR-Cas9 (FAB-CRISPR), which streamlines N/C-terminal tagging using an antibiotic resistance cassette for rapid selection and enrichment of gene-edited cells. We describe in detail guide RNA and HDR donor plasmid cloning, transfection of editin...

A growing anti-sunscreen movement on social media is causing concern among dermatologists, who warn that avoiding sunscreen increases long-term health risks.