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Reposted by Constantinos Patinios

eLife @elife.bsky.social · 13d

Openness means more than access.

We’re pleased to announce that we now publish Replication Studies, helping make science more reliable, transparent, and trustworthy.
buff.ly/Gebypvq

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Constantinos Patinios @copabio.bsky.social · Sep 7

Thank you Jens

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Constantinos Patinios @copabio.bsky.social · Sep 5

Thank you Nicholas 😊

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Constantinos Patinios @copabio.bsky.social · Sep 5

Amazing Martin!! All the best with the new lab and the amazing research you do!!!

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Reposted by Constantinos Patinios

Nature Biotechnology @natbiotech.nature.com · Sep 4

Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes - @helmholtz-hiri.bsky.social go.nature.com/4n7zDpG

Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes - Nature Biotechnology

Append editing of ADP-ribosyl to thymine is used for precise modifications in bacteria and eukaryotes.

go.nature.com

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Constantinos Patinios @copabio.bsky.social · Sep 4

Big shout-out to first authors Darshana Gupta, (myself :) ) & Harris V. Bassett, senior author Chase L. Beisel, and project initiator Scott P. Collins 🙌

Constantinos Patinios @copabio.bsky.social · Sep 4

This opens up brand-new possibilities for precision editing and studying DNA repair!

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Constantinos Patinios @copabio.bsky.social · Sep 4

We developed append editing, a genome-editing strategy that installs ADP-ribose groups onto DNA to unlock new repair outcomes:
🔹 In bacteria → precise, scar-free edits via homologous recombination
🔹 In eukaryotes → unique T→A or T→C base substitutions not possible with current editors

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Constantinos Patinios @copabio.bsky.social · Sep 4

🚀 New publication alert! 🚀

We just published in @natbiotech.nature.com:
👉 Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes
doi.org/10.1038/s415...

Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes - Nature Biotechnology

Append editing of ADP-ribosyl to thymine is used for precise modifications in bacteria and eukaryotes.

doi.org

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Constantinos Patinios @copabio.bsky.social · May 7

Learn more ➡️ www.gmc.vu.lt/en/lsc-embl/...

The Laboratory of Dr. Constantinos Patinios

VU Gyvybės mokslų centras jungia tris VU padalinius – Biomokslų, Biotechnologijos ir Biochemijos institutus. Centras įkurtas vystant integruotą mokslo, studijų ir verslo centrą (slėnį) „Saulėtekis“.

www.gmc.vu.lt

Constantinos Patinios @copabio.bsky.social · May 7

Deadline: Open until a suitable candidate is found.
📧 Send your CV + Motivation Letter to: [email protected]

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Constantinos Patinios @copabio.bsky.social · May 7

🧬 Our work focuses on next-generation genome editing tools for bacteria, yeasts, and phages — with a special focus on DNA replication stalling and repair!

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Constantinos Patinios @copabio.bsky.social · May 7

🌍 Based in beautiful Vilnius, Lithuania
🔬 Access to state-of-the-art equipment and facilities
💼 Competitive salary, personalized mentorship, and cutting-edge research!

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Constantinos Patinios @copabio.bsky.social · May 7

If you’re passionate about genome editing or synthetic biology…
If you want tailored mentorship to drive independent research and launch your career…
This is the place for you! ✨

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Constantinos Patinios @copabio.bsky.social · May 7

🚀 Join the Patinios Lab at the VU LSC - EMBL Partnership Institute for Genome Editing Technologies!
We are looking for a Postdoctoral Researcher to join our growing team! 🔎

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Reposted by Constantinos Patinios

European Research Council (ERC) @erc.europa.eu · Mar 11

Good news on this 35th anniversary of the #Restoration of #Independence in #Lithuania!

A new ERC project from a Lithuanian researcher can now go ahead, as they were able to secure alternative EU funding for her ERC Consolidator Grant.

How did this happen? Find out in the thread 🧵👇

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Constantinos Patinios @copabio.bsky.social · Mar 14

Excited to see our SIBR work out in Trends in Biotechnology :)
doi.org/10.1016/j.ti...

This is the first study I have coordinated from start to finish, and it brings me immense satisfaction to see it published.

For a brilliant description of the tech check this: www.linkedin.com/feed/update/...

Redirecting

doi.org

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Constantinos Patinios @copabio.bsky.social · Nov 25

Huge thanks to Simona Della Valle, Enrico Orsi and Sjoerd Creutzburg for driving this work.

Happy editing :)

Constantinos Patinios @copabio.bsky.social · Nov 25

If you want to use SIBR2.0 for your gene/protein of interest, you can visit the SIBR Site Finder site: colab.research.google.com/drive/162gIZ...

Google Colab

colab.research.google.com

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Constantinos Patinios @copabio.bsky.social · Nov 25

SIBR2.0 can virtually control the expression of any gene/protein of interest, in a wide range of (if not all) mesophilic prokaryotes

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Constantinos Patinios @copabio.bsky.social · Nov 25

By using SIBR2.0-Cas12a we achieved ~70% editing efficiency, in just 48h after electroporation

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Constantinos Patinios @copabio.bsky.social · Nov 25

We show that SIBR2.0 is great for inhibiting the translation of the T7 RNAP in E. coli and then we used it to tightly and inducibly express the Cas12a protein in C. necator

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Constantinos Patinios @copabio.bsky.social · Nov 25

his means that even with the alternative translation site present in the SIBR sequence, a non-functional and split protein will be produced

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Constantinos Patinios @copabio.bsky.social · Nov 25

This is where magic happened!! We developed SIBR2.0 :)
SIBR2.0 includes the introduction of SIBR within the coding sequence of the gene of interest, splitting it into 2 distinct exons

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Constantinos Patinios @copabio.bsky.social · Nov 25

The alternative site for translation appeared to be gene- and organism- specific

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