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Reposted by Rodrigues Lab

Jörg Stülke @joergstuelke.bsky.social · 4d

Identification of genetic interactions in Bacillus subtilis cell division. Congratulations, Byoung-Mo Koo, Carol Gross, and all involved!
@cp-cellsystems.bsky.social #subtiwiki

www.cell.com/cell-systems...

Comprehensive genetic interaction analysis of the Bacillus subtilis envelope using double-CRISPRi

Koo et al. apply genome-scale double-CRISPRi to map cell envelope gene interactions in Bacillus subtilis, revealing >1,000 genetic interactions and uncovering gene networks in envelope biogenesis and ...

www.cell.com

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Rodrigues Lab @crodrigueslab.bsky.social · Aug 30

www.biorxiv.org/content/10.1...

Engineering non-exponential proliferation in Escherichia coli using functionalized protein aggregates

Uncurbed exponential proliferation might not always be required for genetically modified microorganisms, and might even cause unpredictable liabilities in their behavior and impact. We therefore const...

www.biorxiv.org

Reposted by Rodrigues Lab

Ákos T Kovács @evolvedbiofilm.bsky.social · Aug 17

Cryo-EM identifies F-ENA of Bacillus thuringiensis as a widespread family of endospore appendages across Firmicutes

@natcomms.nature.com Mike Sleutel et al from Han Remaut
w/ Marina Aspholm @jerryknowsnothing.bsky.social @mkrupovic.bsky.social @dbaquero.bsky.social

www.nature.com/articles/s41...

Cryo-EM identifies F-ENA of Bacillus thuringiensis as a widespread family of endospore appendages across Firmicutes - Nature Communications

B. thuringiensis spores contain uncharacterized protein filaments that extend from the surface of the exosporium. Here, the authors show that these filaments feature conserved β-barrel neck domains an...

www.nature.com

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Reposted by Rodrigues Lab

Jörg Stülke @joergstuelke.bsky.social · Jul 25

Metals are essential for life, but they can also be toxic. Here, @johnhelmann.bsky.social gives an excellent overview on bacterial metal physiology!
@natrevmicro.nature.com #SubtiWiki

www.nature.com/articles/s41...

Microbial metal physiology: ions to ecosystems - Nature Reviews Microbiology

Metal ions are required for all cells, and their homeostasis relies on ancient mechanisms that facilitate their import, distribution and storage. In this Review, Helmann discusses the key chemical con...

www.nature.com

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Reposted by Rodrigues Lab

STCmicrobeblog @stcmicrobeblog.bsky.social · Jul 22

once upon a time "...the bacterial ribosome was thought to be composed of a fixed set of ribosomal proteins (RPs) and ribosomal RNA (rRNA), ensuring precise translation"

ribosomologists take note: the role of heterogeneity of the ribosomes in the bacterial stress response 👇

#RNASky #MicroSky

Fig 2 Molecular mechanism of differential compositions of RPs and rRNA to respond to external stress. (ⅰ) Antibiotics promote the formation of 61S ribosomes in E. coli both in vitro and in vivo. The specialized ribosomes lacking several proteins such as bS1 and bS21 exhibit structural modifications in the 16S rRNA and exhibit enhanced ability to selectively translate leaderless mRNAs. (ⅱ) Mutations in rpsD, which encodes ribosomal protein S4, have been shown to enhance resistance to oxidative stress and heat in both E. coli and S. typhimurium. (ⅲ) Under toxins, the unstable antitoxin is degraded, leading to the activation of MazF. The MazF system specifically targets the 16S rRNA, cleaving the 3´-terminal 43 nucleotides and anti-Shine-Dalgarno sequence to form 70SΔ43 stress ribosomes, which selectively translate newly generated leaderless mRNAs. (ⅳ) In E. coli, when cells cease growth, a portion of the ribosomal population undergoes dimerization, forming 100S ribosomal dimers that are translationally inactive and thought to be in a hibernation state. (ⅴ) E. coli and S. cerevisiae are prone to mutations in ribosomal proteins uS4 and uS5. Among them, mutations in uS12 are associated with enhanced translational fidelity and increased resistance to streptomycin, while mutations in uS4 usually lead to reduced translational accuracy.

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Rodrigues Lab @crodrigueslab.bsky.social · Jul 10

Nice to see many aspects of my team's research in this review.

www.sciencedirect.com/science/arti...

Compartmentalization during bacterial spore formation

Here, we explore the recent advancements in understanding cellular compartmentalization during bacterial spore formation, primarily focusing on the mo…

www.sciencedirect.com

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Reposted by Rodrigues Lab

Susan Schlimpert @s-lab.bsky.social · Jul 9

Happy to see our collaborative work on the contractile injection system (CIS) in Streptomyces published. We show that the membrane protein CisA gives Streptomyces CIS a licence to kill.
@pilhoferlab.bsky.social & Bastien Casu, @joesallmen.bsky.social #MicroSky

elifesciences.org/articles/104...

Function and firing of the Streptomyces coelicolor contractile injection system requires the membrane protein CisA

CryoEM, genetic, and cell biological analyses reveal that the conserved membrane protein CisA mediates membrane recruitment of contractile injection systems Streptomyces coelicolor, enabling their act...

elifesciences.org

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Rodrigues Lab @crodrigueslab.bsky.social · Jul 4

Thanks Jörg.

Rodrigues Lab @crodrigueslab.bsky.social · Jul 4

Thanks Dirk!

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Rodrigues Lab @crodrigueslab.bsky.social · Jul 4

Thanks for that. Will definitely do that next time!

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Reposted by Rodrigues Lab

Jan-Willem Veening @veeninglab.com · Jun 10

New tenure track assistant professor position in molecular microbiology in our department @dmf-unil.bsky.social @unil.bsky.social! We are casting a wide net for an experimental molecular microbiologist. Apply here: wwwfbm.unil.ch/releve/appli...

DMF: Tenure Track Assistant Professor towards Associate Professor in Molecular Microbiology - Site des postulations FBM

wwwfbm.unil.ch

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Rodrigues Lab @crodrigueslab.bsky.social · Jul 3

Checkout the latest paper from our lab in PLOS Genetics: dx.plos.org/10.1371/jour...

Thanks to our wonderful collaborators, @sgribaldo.bsky.social, @cavalab.bsky.social, @perbullough.bsky.social and Cecile Morlot.

SpoIIIL is a forespore factor required for efficient cell-cell signalling during Bacillus subtilis sporulation

Author summary Bacterial endospores allow bacteria to survive stress, persist in the environment and seed new and recurring infections. Cell-cell signalling is a cornerstone of how bacteria develop in...

dx.plos.org

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Reposted by Rodrigues Lab

Suckjoon Jun’s lab at UCSD @junlab.bsky.social · May 5

Our paper on the E. coli Min system in vivo with @physicsoflifelmu.bsky.social & Judy Kim (UCSD) has been published in Nature Physics today. Tight integration of experiment and theory, and physiology and biophysics. Incredible perseverance of our young students. rdcu.be/ekIpO

Robust and resource-optimal dynamic pattern formation of Min proteins in vivo

Nature Physics - Oscillatory Min protein patterns prevent abnormal bacterial cell division. Now it is shown that Min pattern formation is resource efficient and involves wavelength-invariant...

rdcu.be

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Reposted by Rodrigues Lab

PLOS Biology @plosbiology.org · May 6

How do intracellular #bacteria breach vacuoles to enter the host cytosol? @leaswistak.bsky.social @matthijnvos.bsky.social @enningalab.bsky.social &co show that #Shigella uses its T3SS system to damage endomembranes via mechanoporation to initiate cytosolic access @plosbiology.org 🧪 plos.io/4jXrr9Z

Left: Group of three T3SS localizing in the same zone of the bacteria surface. T3SS n°1 and 2 contact, deform, and perforate the vacuole membrane at the same spot. T3SS n°1 needle (83 nm) is long and seems to deviate from basal body axis showing that strong constraints between T3SS and the vacuole at are play. The locally relaxed vacuole to bacteria space (~50–55 nm) potentially limits additional damage by the T3SS n°3 needle (56 nm) that only slightly deforms the membrane. Right: Model of initial vacuolar membrane breaching by T3SS-induced mechanoporation. Shigella actively enters host cells in a specialized endomembrane compartment, the vacuole, that is rapidly injured and ruptured for cytosolic access. After host cell entry, the vacuole membrane is intact and tightly juxtaposed to the surface of Shigella, permitting contact between T3SSs and the endomembrane. In the authors’ proposed model, vacuole membrane injury is supported both by the vacuole tightness and the length of individual T3SSs.

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Rodrigues Lab @crodrigueslab.bsky.social · May 6

Checkout out our computational framework for studying toroidal membranes of biological dimensions, present at division and fission sites. We studied lipid behaviour at these sites but also simulated membrane fission. Collaboration with @chrislbgraham.bsky.social and @pstansfeld.bsky.social

Chris LB Graham @chrislbgraham.bsky.social · May 3

Just released a pre-print: www.biorxiv.org/content/10.1... We simulate lipid membrane toroids of bacteria, representing the point where one cell splits into two. We see they are stable and then look at how a key protein might behave at them @crodrigueslab.bsky.social @pstansfeld.bsky.social

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Reposted by Rodrigues Lab

PLOS Biology @plosbiology.org · Apr 22

The unique #CellEnvelope of #corynebacteria: @tbharat-lab.bsky.social &co map the C. glutamicum cell surface, revealing a patchy S-layer & specific assembly of the PS2 protein, thereby informing our understanding of cell envelopes that contain #MycolicAcids @plosbiology.org 🧪 plos.io/3Y6JNgw

Left: The atomic model of the PS2 lattice is shown in top and side views. PS2 hexamers are repeated in the 2D sheet with a lattice constant of 176 Å. Each PS2 monomer is colored and labeled separately within the hexamer, which repeats throughout the lattice (with pore dimensions highlighted). The side view of the lattice shows the smooth surface facing the extracellular space and rough surface with coiled-coil segments protruding towards the MM. Top right: Interactions at the hexameric and trimeric interfaces are stabilized by both hydrophilic and hydrophobic residues. Bottom right: A cell envelope tomogram was used to overlay the cryo-EM structure of the S-layer onto the in situ S-layer tomographic density. A single slice of the tomogram is shown, with the overlayed PS2 S-layer lattice in green and the tomogram in grayscale. The S-layer (surface layer), MM (mycomembrane), IM (inner membrane), and Cytosol are labeled.

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Reposted by Rodrigues Lab

Eduardo Rocha @epcrocha.bsky.social · Apr 16

New postdoc position in our lab (2 y+): evolutionary genomics of integrons and MGEs with focus on vibrio-phage interactions. Great environment @pasteur.fr for science, career building. Super collaborators @celineloot.bsky.social @amazeld.bsky.social @fredoleroux.bsky.social 3 weeks to apply!

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Reposted by Rodrigues Lab

Pooja Gupta @poojag96.bsky.social · Mar 18

The preprint is out now - we describe how Bacillus spores restart energy metabolism as they exit dormancy! Dormant spores are dehydrated, very robust for long periods, but when they sense nutrient availability, they can germinate rapidly to make the most of the favourable environmental conditions 1/

bioRxiv Biophysics @biorxiv-biophys.bsky.social · Mar 16

Bioenergetic metabolism restarts alongside germinant sensing and hydration in bacterial spore germination https://www.biorxiv.org/content/10.1101/2025.03.13.642030v1

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Reposted by Rodrigues Lab

davidroper2.bsky.social @davidroper2.bsky.social · Apr 8

Easter is approaching but so is the abstract deadline for the Great Wall Symposium: May 1st!
If you want a chance to present at the best conference, focused on the Bacterial Cell Envelope, crack on!
Sicily, September 15-17th 2025

thegreatwall-symposium.org

The Greatwall-symposium

The Great Wall Symposium will take place from 18th to 20th September 2023 at the venue in The Vila Galé Sintra Hotel, in Portugal.

thegreatwall-symposium.org

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Reposted by Rodrigues Lab

Anjana Badrinarayanan @anjbadri.bsky.social · Apr 4

Happy to share Asha Joseph and Inchara Adhikashreni's latest work from the lab, describing replisome speed variation in Caulobacter!

www.cell.com/current-biol...

Live tracking of replisomes reveals nutrient-dependent regulation of replication elongation rates in Caulobacter crescentus

Adhikashreni et al. use quantitative live-cell imaging to track cell-cycle dynamics in hundreds of individual bacteria. They observe nutrient-dependent regulation of replication elongation rates that ...

www.cell.com

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Reposted by Rodrigues Lab

MRC Laboratory of Molecular Biology @mrclmb.bsky.social · Mar 31

How do SMC complexes capture DNA? 🧪

Led by Frank Bürmann, Jan Löwe’s & Mark Dillingham’s groups have identified the directional loading mechanism the bacterial SMC complex MukBEF uses to capture & ingest DNA, & found a phage inhibitor to this pathway.

Read more: tinyurl.com/4s8nrrkk

#LMBResearch

Schematic illustrating the structural changes undergone by MukBEF SMC complex during DNA capture and ingestion.

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Reposted by Rodrigues Lab

Molecular Microbiology Editors @molecularmicro.bsky.social · Mar 31

Competent streptococci release hydrolases associated with fratricide (bacteria kill closely related cells to release DNA). The ScrI protein substitutes Thr for Ala in peptidoglycan cross bridges to confer immunity!
#MicroSky #Peptidoglycan #Competence

onlinelibrary.wiley.com/doi/10.1111/...

Self‐Immunity Towards a Novel Competence‐Induced Streptococcal Peptidoglycan Hydrolase is Mediated by a Fem‐Transferase‐Like Protein

ScrM is a novel competence-induced peptidoglycan hydrolase with activity against pyogenic streptococci. ScrM binds specifically to midcell of sensitive bacteria. Immunity to ScrM is mediated by a ded...

onlinelibrary.wiley.com

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Reposted by Rodrigues Lab

Ákos T Kovács @evolvedbiofilm.bsky.social · Apr 1

Bacterial growth under pressure: the surprising role of membrane microdomains

Behind the paper by Dennis Claessen on their recent @naturecomms.bsky.social publication

communities.springernature.com/posts/bacter...

Kitasatospora viridifaciens

Bacterial growth under pressure: the surprising role of membrane microdomains

communities.springernature.com

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Reposted by Rodrigues Lab

PLOS Biology @plosbiology.org · Mar 26

The term "reviewer fatigue” has become familiar in scientific publishing. In this Editorial, @droutledge.bsky.social & @npariente.bsky.social discuss how we can make the peer review system more sustainable & ways that PLOS Biology is already helping to ease the burden on reviewers🧪
▶️ plos.io/41YKIRa

On improving the sustainability of peer review

The term “reviewer fatigue” has become only too familiar in scientific publishing. This editorial discusses how we can ease the burden on reviewers to make the peer review system more sustainable, whi...

plos.io

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Reposted by Rodrigues Lab

Ákos T Kovács @evolvedbiofilm.bsky.social · Mar 23

Have you ever seen such a huge biofilm deficiency with bslA mutant of 3610, @bacteriacities.bsky.social?

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