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Tristan Croll @crolltristan.bsky.social · Aug 13

Mashing Pumpkins

Tristan Croll @crolltristan.bsky.social · Jul 6

Oooh, I know lots of those!

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Tristan Croll @crolltristan.bsky.social · Jun 12

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Tristan Croll @crolltristan.bsky.social · May 5

Possibly the most evergreen Onion article in history: theonion.com/recession-pl...

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Tristan Croll @crolltristan.bsky.social · Mar 28

“What is biology?”

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Tristan Croll @crolltristan.bsky.social · Mar 3

This brings back memories… climbing at Mt Arapiles, near the top-out about 30m off the ground I came across a crack very like this, with an odd white spot deep inside. Got closer to look, and realised it was a teeny tiny pair of fangs… unforgettable moment.

A small, scared bat showing off its fangs.

Tristan Croll @crolltristan.bsky.social · Jan 30

I’m guessing your problem is the dependency on the “sanity” package. Unfortunately that stopped being maintained a while back, and won’t install in the latest Python version.

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Reposted by Tristan Croll

Sam "The Mug Guy" Horrell @drhorrell.bsky.social · Jan 8

Had a very long social media break after the last #SynchrotronMugMadness and the bird site's decent into horror.

So hello bluesky, I'm a staff structural biologist helping @imperiallifesci.bsky.social solve protein structures with #crystallography and #CryoEM.

Please enjoy these protein crystals 💎

Several microscopic iron containing protein crystals in a variety of cookies (blue, yellow, orange, green)

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Tristan Croll @crolltristan.bsky.social · Jan 4

🎶 When a muppet with teef finds a home on the reef, that’s a moray 🎵

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Tristan Croll @crolltristan.bsky.social · Dec 31

(this is actually the old laptop I used for most of my original work on ISOLDE. Poor little thing… will try to look after it better in future)

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Tristan Croll @crolltristan.bsky.social · Dec 31

Kids were complaining that their gaming laptop was getting a bit sluggish… 😳🤢

A solid wall of accumulated dust. Somewhere underneath it is a laptop cooling fan.

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Tristan Croll @crolltristan.bsky.social · Dec 22

Interviewer: Can you explain this gap on your CV?

Evolutionary biology: Well, yeah… but then I’ll have two *new* gaps you’ll want me to explain.

Nessa Carson @supersciencegrl.co.uk · Dec 22

Interviewer: Can you explain this gap on your CV?

Chemist: Yeah, that's what's between the HOMO and the LUMO #chemsky

Leo C. Stein @duetosymmetry.com · Dec 22

Interviewer: Can you explain this gap in your resume?

Gravitational-wave astronomer: It depends on the maximum neutron star mass and details of the supernova mechanism ... or it could just be a selection effect

🧪⚛🔭

Tristan Croll @crolltristan.bsky.social · Dec 21

Lady Cassandra??

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Tristan Croll @crolltristan.bsky.social · Dec 21

Could of course fire up a “proper” docking package for that, but often this way seems quicker and easier (and helps get a better feel for the site, to boot). Take out the map, “isolde restrain distances” to maintain local conformation, then just play with it.

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Tristan Croll @crolltristan.bsky.social · Dec 21

Both true. :) Although if it’s a novel ligand (or just something not in the CCD) the first point isn’t such a big issue. I don’t use it *that* often, but I do occasionally use it quickly assess hypotheses (“I wonder if{ligand} might plausibly fit this pocket?”).

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Reposted by Tristan Croll

Clausen Lab @clausenlab.bsky.social · Dec 21

🎄 xmas preprint 🎄 we are excited to share our cryo-EM structure of UBR4 in complex with KCMF1 and CALM1. the PQC ligase forms a massive ubiquitination arena, primed to amplify ubiquitin chains (E4 activity) and boost degradation of defective proteins. www.biorxiv.org/content/10.1...

Architecture of the 1.3 MDa complex of human UBR4 (2 x UBR4/KCMF1/CALM1). Picture shows top and side views of the cryo-EM density map and the modeled structure, colored by protein components and domains.

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Tristan Croll @crolltristan.bsky.social · Dec 20

Still trying to work that out, I’m afraid. While we have some interesting “ISOLDE-adjacent” stuff in the works, my day job currently doesn’t leave a lot of (well… any, really) time free for development of ISOLDE itself. Exploring options to support it in a more sustainable way.

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Tristan Croll @crolltristan.bsky.social · Dec 20

One nice quality-of-life addition: all “open” commands now have an optional “cofr” argument. So for example…

open ligand.mol2 center cofr

or

open {smiles string} from smiles center cofr

… will put the ligand on ISOLDE’s crosshairs ready to move around and merge in.

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Tristan Croll @crolltristan.bsky.social · Dec 20

While I haven’t had a chance to add anything really substantial to ISOLDE, the @www.cgl.ucsf.edu team have added a bunch of great new stuff to ChimeraX itself.

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Tristan Croll @crolltristan.bsky.social · Dec 20

For people who managed to install older versions of ISOLDE into ChimeraX and found they promptly failed… sorry, that’s my fault. I didn’t RTFM about Python’s ‘~=‘ specifier. Thought “~=1.8.rc0” meant “>=1.8.rc0”, ==1.8.* when it actually means “>=1.8.rc0, ==1.*”.

🙃

Anyway, update to fix.

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Tristan Croll @crolltristan.bsky.social · Dec 20

You know what they say about never releasing on a Friday… what does it say about me that I’m releasing on the last Friday before Christmas? 😬

Anyway, ISOLDE builds for ChimeraX 1.9 are now up. Enjoy!

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Reposted by Tristan Croll

Derek Lowe @dereklowe.bsky.social · Dec 18

An interesting finding that some types of HPV infection might be preventing cancer (as opposed to the ones that can cause it).

Also features G.K. Chesterton and a brief cameo by RFK Jr.

HPV Infections: Friend and Foe

www.science.org

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Tristan Croll @crolltristan.bsky.social · Dec 16

Man… the fact that there’s a *second* ring galaxy visible in the gap? The sci-fi story (where these are the still-growing explosions from the past firing of some unimaginably powerful galaxy-killing beam weapon) practically writes itself.

UniverseDiscovery🌌🔭 @creatorbluesky.bsky.social · Dec 16

Hoag’s Object is a rare ring galaxy located over 600 million light-years from Earth.

A nearly perfect circle full of young blue stars surrounds a spheroid of mostly older red giant stars. In between is a gap that appears almost completely empty.

Delightfully distinctive.

📸 Credit: NASA/STScl/AURA

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Tristan Croll @crolltristan.bsky.social · Dec 16

I’m afraid I honestly don’t remember that. 🤷‍♂️

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Tristan Croll @crolltristan.bsky.social · Dec 16

I remember Jane Richardson and her team doing a fair bit of work trying to sort out “total junk” from “partially correct” down in the very-low-pLDDT regime. Don’t know if/where it was published, though.

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