Multi-scale Molecular Imaging of Human Cells reveals COPI and COPII Vesicles at ER Exit Sites https://www.biorxiv.org/content/10.1101/2025.07.29.667472v1

Kangfu Peng, Hong Zhang et al. show that scramblase-attenuated #Celegans ATG-9 mutants rescue the #autophagy defect and lysosome damage caused by nondegradative autolysosomes. This uncovers the role of ATG-9 in regulating #lysosome function & integrity. rupress.org/jcb/article/...

@ellieattridge.bsky.social @philipparosewell.bsky.social @kleoal.bsky.social @ealmacellas.bsky.social @alinaueffing.bsky.social @satooze.bsky.social

I’m so grateful for all my wonderful colleagues in the Molecular Cell Biology of Autophagy Lab, who inspire, challenge and support me every day. Together we’re making an impact, breaking barriers and shaping the future of science #womeninSTEM #internationaldayofwomanandgirlsinscience

It’s incredibly hard to study lipids in biological membranes on the nanoscale. You need near-perfect information on both membrane ultrastructure and lipid density, which usually isn’t attainable. Lipid-CLEM brought to you by @mathilda95.bsky.social changes that.
www.biorxiv.org/content/10.1...

Just before the New Year: BindCraft v1.5! Please fill out this form to request new features and help us make BindCraft even better.
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How proximal is proximity labeling❓ Using DNA nanorulers we found that TurboID/BioID are largely contact labeling, while APEX2 also bears a major contact labeling pathway in parallel with diffusive labeling.
www.biorxiv.org/content/10.1...

Special thanks to @aliceyting.bsky.social for discussions

Look!
Mechanistic studies of autophagic cargo recruitment and membrane expansion through in vitro reconstitution

biorxiv.org/cgi/content/...