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Reposted by Fidel Ramírez-Amador

Sven T. Stripp @stripplab.bsky.social · 2d

Happy to share our latest review article, this time joining forces with 📌Seigo Shima, 📌James Birrell, 📌Giorgio Caserta, and 📌Oli Lenz. We wrote about hydrogenase catalysis because this is what we do best 😎
doi.org/10.1002/9783...

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Mike Jetten @msmjetten.bsky.social · 19d

#Methanosarcina acetivorans requires methanol:coenzyme M methyltransferases for #ethane formation from ethanol #methane
link.springer.com/article/10.1...

Methanosarcina acetivorans requires methanol:coenzyme M methyltransferases for ethane formation from ethanol - Antonie van Leeuwenhoek

Corrinoid-dependent enzymes either catalyze methyltransfer reactions, or they generate substrate radicals using adenosylcobalamin for subsequent rearrangement reactions. The corrinoid-dependent methyl...

link.springer.com

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Evgenii Protasov @evgenii-protasov.bsky.social · 12d

New frontiers in short-chain alkyl-coenzyme M reductases
#methanogenesis #methane #microbiology
www.sciencedirect.com/science/arti...

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bioRxiv Microbiology @biorxiv-microbiol.bsky.social · 24d

Carbon monoxide metabolism in freshwater anaerobic methanotrophic archaea https://www.biorxiv.org/content/10.1101/2025.09.16.676500v1

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Evgenii Protasov @evgenii-protasov.bsky.social · Sep 9

Atomic resolution structures of the methane-activating enzyme in anaerobic methanotrophy reveal extensive post-translational modifications
#microbiology #methane #archaea
@natcomms.nature.com
www.nature.com/articles/s41...

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James Lingford @jameslingford.bsky.social · Sep 1

Fantastic review on methodological developments in protein structural phylogenetics
academic.oup.com/gbe/article/...

Protein Structural Phylogenetics

Abstract. Protein structural phylogenetics is an interdisciplinary branch of molecular evolution that (i) uses 3D structural data to trace evolutionary his

academic.oup.com

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Sven T. Stripp @stripplab.bsky.social · Aug 21

Stepwise and reversible assembly of [2Fe–2S] rhombs to [8Fe–8S] clusters (Mougel et al. via @natchem.nature.com)
www.nature.com/articles/s41...

Stepwise and reversible assembly of [2Fe–2S] rhombs to [8Fe–8S] clusters and their topological interconversions - Nature Chemistry

Elucidating the nature of the metallocofactors in nitrogenase enzymes, and preparing synthetic analogues of these clusters, is a classic target for bioinorganic chemists. Now the transformation of [Fe...

www.nature.com

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Chris Greening @greening.bsky.social · Aug 8

1/3 Electricity from air! In the top journal Nano Energy today, we report the first fuel cell that makes power from air alone. We achieved this by using the enzyme Huc to oxidize ambient hydrogen. This results in a continuous low-level power from air and higher outputs with industrial waste gases.

Nanoengineered bioanode with oxygen-insensitive hydrogenase for sustainable energy harvesting from atmospheric hydrogen and waste gases

Enzymatic biofuel cells (EBFCs) utilizing hydrogenases to oxidize hydrogen offer a sustainable approach to energy conservation. However, the oxygen se…

www.sciencedirect.com

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John Helmann @johnhelmann.bsky.social · Jul 28

Metal ions are universally required for life, and many of the foundational principles of metal homeostasis have emerged from studies of microbial systems. In this review, I provide a introductory overview targeted to those new to the field.
#MicroSky #Metals

rdcu.be/eycU2

Microbial metal physiology: ions to ecosystems

Nature Reviews Microbiology - Metal ions are required for all cells, and their homeostasis relies on ancient mechanisms that facilitate their import, distribution and storage. In this Review,...

rdcu.be

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Evgenii Protasov @evgenii-protasov.bsky.social · Jul 3

Our new study shows how nickel limitation changes electron flow in methanogens.
@nature.com
#methanogens #methane #archaea
www.nature.com/articles/s41...

Electron flow in hydrogenotrophic methanogens under nickel limitation - Nature

Two distantly related methanogens shift the methanogenic pathway under nickel limitation, suggesting that methanogens adapt to changing bioavailability of nickel by rerouting electron flow between two...

www.nature.com

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georghochberg.bsky.social @georghochberg.bsky.social · Jun 11

New paper from the lab from Sriram Garg in my group. We introduce a general substitution matrix for structural phylogenetics. I think this is a big deal, so read on below if you think deep history is important. academic.oup.com/mbe/advance-...

A general substitution matrix for structural phylogenetics.

Abstract. Sequence-based maximum likelihood (ML) phylogenetics is a widely used method for inferring evolutionary relationships, which has illuminated the

academic.oup.com

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Schuller Jan @schullerjm.bsky.social · Jun 4

Proud to present the lab's latest work. The full structure of the sodium translocating methyltransferase (Mtr) bound to a small oxygen-responsive small protein MtrI. Work by Tristan and together with @schmitzstreitslab.bsky.social .

www.biorxiv.org/content/10.1101/2025.06.02.657420v1

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Sven T. Stripp @stripplab.bsky.social · Jun 4

CLUSTERING FOR METHANE
Nice write-up @natcatal.nature.com of our recent article on MCR activation 🙏🏻
www.nature.com/articles/s41...

Clustering for methane - Nature Catalysis

Nature Catalysis - Clustering for methane

www.nature.com

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Johannes Rebelein @rebeleinlab.bsky.social · Jun 1

Leveraging spectroscopic and structural approaches we obtained new insights into the role of #ferredoxins in N2 fixation. Just published in Chemistry – A European Journal: doi.org/10.1002/chem...! Great work by Holly Addison. Fantastic collaboration with Antonio Pierik on the EPR characterization.

Two Key Ferredoxins for Nitrogen Fixation Have Different Specificities and Biophysical Properties

Two ferredoxins, FdC and FdN, are collectively essential for the process of nitrogen fixation by the iron nitrogenase in R. capsulatus. We explore the biophysical factors, through spectroscopic and s....

doi.org

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Schuller Jan @schullerjm.bsky.social · May 12

Happy to present the lab latest work, today from the green-side. The ultimate PSII assembly intermediate that directly precedes incorporation of the Mn4O5Ca cluster and formation of the OEC. Psb32 binds to PsbV, challenging the assumption the extrinsic subunits all associate spontaneously in-vivo.

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Duncan Kountz @dkountz.bsky.social · May 2

My lab mates and their collaborators have a really cool paper you should check out: the structure of catalytically active (Ni(I)) methyl-coM reductase: www.biorxiv.org/content/10.1...

www.biorxiv.org

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Sven T. Stripp @stripplab.bsky.social · Apr 17

Short press piece @unipotsdam.bsky.social about our new article @nature.com on methyl coenzyme M reductase (MCR)
www.uni-potsdam.de/en/headlines...

Early life – Enzyme for biological methane production decoded

www.uni-potsdam.de

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Technische Universität Berlin @tuberlin.bsky.social · Apr 25

🧪 Durchbruch in der biochemischen Grundlagenforschung mit zentraler Bedeutung für #Energie & #Klima:
Ein Team der @unimarburg.bsky.social & der #TUBerlin/ @unisyscat.bsky.social hat erstmals den Aktivierungsmechanismus des Enzyms MCR aufgeklärt – zentral für die biologische Produktion von #Methan. 👇

Durchbruch in der Methan-Forschung: Aktivierungsmechanismus des Methan-produzierenden Enzyms entschlüsselt

„Nature“-Publikation offenbart die evolutionäre Verbindung zwischen zwei fundamentalen biologischen Prozessen

www.tu.berlin

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Fidel Ramírez-Amador @fidelormz.bsky.social · Apr 16

This project was possible thanks to the great team formed with my shared co-first authors @sophia-pa.bsky.social and @rnfr2d2.bsky.social and the supervision of @schullerjm.bsky.social plus awesome contributions, specially from @stripplab.bsky.social @georghochberg.bsky.social and Silvan Scheller.

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Fidel Ramírez-Amador @fidelormz.bsky.social · Apr 16

Amazingly, our phylogenetic reconstructions traced the origin of these clusters and their protein scaffolds in methanogens before being repurposed for nitrogen fixation.

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Fidel Ramírez-Amador @fidelormz.bsky.social · Apr 16

Last but not least, three iron-sulfur clusters shape an electron transfer pathway towards the exposed coenzyme F430. Unexpectedly, these clusters are reminiscent of the [8Fe-9S-C] cofactors (or L-clusters) that so far had only been found in the nitrogenase family.

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Fidel Ramírez-Amador @fidelormz.bsky.social · Apr 16

These changes allow CoB-SH to descend and form the heterodisulfide by-product with CoM. Whereas the more distal active site is still rigid while holding the substrates.

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Fidel Ramírez-Amador @fidelormz.bsky.social · Apr 16

But how is the catalytic pocket of MCR remodelled during activation? When complexed to the other components, the more 'proximal' half of MCR undergoes drastic conformational changes to expose the Ni-center in coenzyme F430, likely facilitating its further reduction.

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Fidel Ramírez-Amador @fidelormz.bsky.social · Apr 16

To show the ATP-dependency activation of MCR, we followed the CH4 production at different scenarios (as isolated, ATP depletion, non-hydrolyzable ATP analogues). The external addition of ATP not only boosts the generation of CH4 but also promotes the interaction of A2 with MCR.

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Fidel Ramírez-Amador @fidelormz.bsky.social · Apr 16

We used redox-controlled cryoEM to obtain different functional states (local resolutions as high as 1.8 angstrom!) of the activation complex asymetrically bound to MCR. The small McrC along with several methanogenic marker proteins (Mmp) and A2 (an ATPase) compose the complex.

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