hansenjn.bsky.social
hansenjn
@hansenjn.bsky.social
87 followers 160 following 4 posts
Shedding light on #cilia, #flagella, and subcellular biology. Developing open-source image analysis for subcellular structures and whole cells (adipocytes, #microglia, #macrophage, and all kinds of other cells): CiliaQ, SpermQ, AdipoQ, MotiQ, FreQ, ...
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hansenjn.bsky.social
hansenjn @hansenjn.bsky.social · 11d
So proud and happy to share that our study on the #proteome, heterogeneity, and cell-type specificity of primary #cilia is published now at Cell 🎉

Big thank you to all the many people that made this possible, at the Lundberg Lab, Human Protein Atlas, Lindstrand lab, and Hsien-Sheu lab.
cp-cell.bsky.social
Cell - a Cell Press journal @cp-cell.bsky.social · 12d
Now online! Intrinsic heterogeneity of primary cilia revealed through spatial proteomics
Intrinsic heterogeneity of primary cilia revealed through spatial proteomics
An antibody-based spatial proteomics screen on human primary cilia explores 715 proteins across single cilia of different cell types and reveals extensive cell-type specificity, single-cilium heterogeneity, ciliary microdomains, CFAP300 as a new cilium marker protein, 91 novel proteins including CREB3, and a CREB3 variant associated to ciliopathy phenotypes.
dlvr.it
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Reposted by hansenjn
proteinatlas.bsky.social
Human Protein Atlas @proteinatlas.bsky.social · 13d
In the journal Cell a deep analysis of the HPA primary cilia section is published. The primary cilia is portrayed as the most heterogeneous organelles, customized by the cell for fine-tuned environmental sensing, inspiring new views on this tiny antenna.

www.proteinatlas.org/news/2025-09...
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hansenjn.bsky.social
hansenjn @hansenjn.bsky.social · Mar 3
Could I join as well?
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hansenjn.bsky.social
hansenjn @hansenjn.bsky.social · Feb 2
Or, if the protein exists in humans, you could check whether the human protein atlas contains antibody stainings for the protein on human tissues with motile cilia (www.proteinatlas.org/humanproteom...) and whether you can observe a similar phenomenon there as well.
The human proteome - The Human Protein Atlas
www.proteinatlas.org
hansenjn.bsky.social
hansenjn @hansenjn.bsky.social · Feb 2
Exciting! I'd be curious whether a corresponding human protein exists. If yes, you may check whether it was by chance stained in ciliated human cells in the human protein atlas and whether you see diversity between different cells: www.proteinatlas.org. Preprint: www.biorxiv.org/content/10.1....
Intrinsic Diversity In Primary Cilia Revealed Through Spatial Proteomics
Primary cilia are a critical organelle found on most human cells, and their dysfunction is linked to hereditary ciliopathies with diverse symptoms. Despite their significance, the specific roles of ci...
www.biorxiv.org
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Reposted by hansenjn
mick-lab.bsky.social
David Mick @mick-lab.bsky.social · Jan 11
Proud to share our work spear-headed by PhD student Tommy Sroka with major contributions by #xenopus expert Kerstin Feistel.

Our iAPEX #ProximityLabeling method for #MassSpec based subcellular #proteomics works by locally generating H2O2 using a D-amino acid oxidase that activates APEX2 in situ.
iAPEX: Improved APEX-based proximity labeling for subcellular proteomics using an enzymatic reaction cascade
Ascorbate peroxidase (APEX) is a versatile labeling enzyme used for live-cell proteomics at high spatial and temporal resolution. However, toxicity of its substrate hydrogen peroxide and background la...
www.biorxiv.org
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Reposted by hansenjn
cilialab.bsky.social
Mill_lab @cilialab.bsky.social · Dec 30
Calendars out- just over two weeks until our 🎉50th🥳 @bscb-official.bsky.social @gensocuk.bsky.social @ukcilianetwork.bsky.social e-symposium 14/01/2025 15:00-17:30- everything hot in #cilia #centrosomes free & open to all! Register to access this 🔥 series: www.eventbrite.co.uk/e/bscb-genso... /1
Advert for the e-symposium with list of speaker, affiliations and imagegs of cilia and centrioles. Line up of our speakers, affiliations and talk titles for our 50th event!
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