Landon Clark
@landonclark.bsky.social
MD/PhD Student at Vanderbilt University School of Medicine interested in Germinal Centers, B cells, and Autoimmunity | MPhil @ University of Cambridge | Vision Zero Nashville
Reposted by Landon Clark
I am excited to share that our work, in which we have dissected the signals driven by the nucleoside-modified mRNA and LNP components of mRNA vaccines that regulate germinal center (GC) responses, is now published in Cell! www.cell.com/cell/fulltex...
Distinct components of mRNA vaccines cooperate to instruct efficient germinal center responses
This study dissects the individual contributions of the different components of mRNA
vaccines to immune responses. Nucleoside-modified mRNA promotes the production of
type I interferons that act on de...
www.cell.com
December 16, 2025 at 5:40 PM
I am excited to share that our work, in which we have dissected the signals driven by the nucleoside-modified mRNA and LNP components of mRNA vaccines that regulate germinal center (GC) responses, is now published in Cell! www.cell.com/cell/fulltex...
Reposted by Landon Clark
T follicular helper cells adopt alter egos and localize to diverse niches - what gene expression programs distinguish Tfh in the germinal center? After dissociating tissue samples into single-cell suspension, can we infer which Tfh were positioned in GCs? Check out our new preprint! bit.ly/3XVsrCI
Multimodal analysis defines GNG4 as a distinguishing feature of germinal center-positioned CD4 T follicular helper cells in humans
CD4 T follicular helper (Tfh) cells coordinate humoral immune responses within germinal centers (GC) of lymphoid tissue. Despite their critical roles in vaccination and autoimmunity, the gene expressi...
bit.ly
December 15, 2025 at 5:03 PM
T follicular helper cells adopt alter egos and localize to diverse niches - what gene expression programs distinguish Tfh in the germinal center? After dissociating tissue samples into single-cell suspension, can we infer which Tfh were positioned in GCs? Check out our new preprint! bit.ly/3XVsrCI
Reposted by Landon Clark
This #Preprint reports that B6 (but not wild mice) have a truncated PDL2, missing an active intracellular signalling domain:
"Surprisingly, in C57BL/6J (B6) mice PD-L2 has only a 5-amino acid intracellular domain, and the same is true for several other laboratory mouse strains"
#Immunology
"Surprisingly, in C57BL/6J (B6) mice PD-L2 has only a 5-amino acid intracellular domain, and the same is true for several other laboratory mouse strains"
#Immunology
PD-L2 Regulates Natural Antibody and IL-10 Secretion by B-1 cells
B-1 cells are innate-like lymphocytes that play a critical role in homeostasis by secreting natural antibodies, typically IgM, and immunosuppressive molecules such as IL-10. However, the regulation of...
www.biorxiv.org
December 15, 2025 at 6:03 AM
This #Preprint reports that B6 (but not wild mice) have a truncated PDL2, missing an active intracellular signalling domain:
"Surprisingly, in C57BL/6J (B6) mice PD-L2 has only a 5-amino acid intracellular domain, and the same is true for several other laboratory mouse strains"
#Immunology
"Surprisingly, in C57BL/6J (B6) mice PD-L2 has only a 5-amino acid intracellular domain, and the same is true for several other laboratory mouse strains"
#Immunology
Reposted by Landon Clark
Review @natrevrheum.nature.com @clevelandclinic.bsky.social
Emerging and underrecognized viral triggers of autoimmune inflammatory rheumatic disease flares
www.nature.com/articles/s41...
Emerging and underrecognized viral triggers of autoimmune inflammatory rheumatic disease flares
www.nature.com/articles/s41...
December 8, 2025 at 6:44 PM
Review @natrevrheum.nature.com @clevelandclinic.bsky.social
Emerging and underrecognized viral triggers of autoimmune inflammatory rheumatic disease flares
www.nature.com/articles/s41...
Emerging and underrecognized viral triggers of autoimmune inflammatory rheumatic disease flares
www.nature.com/articles/s41...
Reposted by Landon Clark
Altered immune and metabolic molecular pathways drive islet cell dysfunction in human type 1 diabetes @jci.org @bcellorg.bsky.social @ualberta.bsky.social #T1D
www.jci.org/articles/vie...
www.jci.org/articles/vie...
www.jci.org/articles/vie...
www.jci.org/articles/vie...
December 2, 2025 at 6:32 PM
Altered immune and metabolic molecular pathways drive islet cell dysfunction in human type 1 diabetes @jci.org @bcellorg.bsky.social @ualberta.bsky.social #T1D
www.jci.org/articles/vie...
www.jci.org/articles/vie...
www.jci.org/articles/vie...
www.jci.org/articles/vie...
Reposted by Landon Clark
Joint chromatin accessibility and transcriptomic profiling of human and mouse pancreatic lymph nodes uncovers distinct features of CD4 T cells in T1D @sciimmunology.bsky.social @vahedilab.bsky.social
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
November 21, 2025 at 7:41 PM
Joint chromatin accessibility and transcriptomic profiling of human and mouse pancreatic lymph nodes uncovers distinct features of CD4 T cells in T1D @sciimmunology.bsky.social @vahedilab.bsky.social
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Officially PhD candidate, passed my qual 🥳🥳🥳
November 21, 2025 at 9:51 PM
Officially PhD candidate, passed my qual 🥳🥳🥳
Reposted by Landon Clark
@biorxivpreprint.bsky.social B-cell follicles act as a critical microanatomical niche for sustained viral replication, virus-host interaction, and tissue damage during SIV chronic infection
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
November 21, 2025 at 2:30 AM
@biorxivpreprint.bsky.social B-cell follicles act as a critical microanatomical niche for sustained viral replication, virus-host interaction, and tissue damage during SIV chronic infection
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Landon Clark
🧪For anyone interested in EBV in autoimmunity, I strongly urge you to read this incredible study by @shady-myounis.bsky.social, Bill Robinson, and @tobiaslanz.bsky.social. It is not only a scientific tour de force, it has a deeply provocative discussion that everyone in the field should consider 1/
Epstein-Barr virus reprograms autoreactive B cells as antigen-presenting cells in systemic lupus erythematosus
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Epstein-Barr virus reprograms autoreactive B cells as antigen-presenting cells in systemic lupus erythematosus
Epstein-Barr virus reprograms autoreactive B cells as antigen-presenting cells to promote pathogenic antinuclear T and B cell responses in lupus.
www.science.org
November 19, 2025 at 5:24 PM
🧪For anyone interested in EBV in autoimmunity, I strongly urge you to read this incredible study by @shady-myounis.bsky.social, Bill Robinson, and @tobiaslanz.bsky.social. It is not only a scientific tour de force, it has a deeply provocative discussion that everyone in the field should consider 1/
Reposted by Landon Clark
November 18, 2025 at 4:00 PM
Reposted by Landon Clark
Antigen presenting cells play a critical role in determining response to regulatory T cell therapy in type 1 diabetes https://www.biorxiv.org/content/10.1101/2025.11.15.688620v1
November 16, 2025 at 11:17 PM
Antigen presenting cells play a critical role in determining response to regulatory T cell therapy in type 1 diabetes https://www.biorxiv.org/content/10.1101/2025.11.15.688620v1
Reposted by Landon Clark
CD21 initiates extrafollicular differentiation of autoantibody-secreting cells in an SLE model driven by TLR7 signaling @sciimmunology.bsky.social @bostonchildrens.bsky.social
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
November 15, 2025 at 3:24 AM
CD21 initiates extrafollicular differentiation of autoantibody-secreting cells in an SLE model driven by TLR7 signaling @sciimmunology.bsky.social @bostonchildrens.bsky.social
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Landon Clark
www.science.org/doi/10.1126/...
“These EBV-infected B cells with antigen-presenting abilities had the capacity to activate autoreactive helper T cells, setting off a chain reaction where those T cells could activate other autoreactive B cells, including uninfected ones.”
#SLE #Bcell #EBV
“These EBV-infected B cells with antigen-presenting abilities had the capacity to activate autoreactive helper T cells, setting off a chain reaction where those T cells could activate other autoreactive B cells, including uninfected ones.”
#SLE #Bcell #EBV
Epstein-Barr virus reprograms autoreactive B cells as antigen-presenting cells in systemic lupus erythematosus
Epstein-Barr virus reprograms autoreactive B cells as antigen-presenting cells to promote pathogenic antinuclear T and B cell responses in lupus.
www.science.org
November 13, 2025 at 2:46 AM
www.science.org/doi/10.1126/...
“These EBV-infected B cells with antigen-presenting abilities had the capacity to activate autoreactive helper T cells, setting off a chain reaction where those T cells could activate other autoreactive B cells, including uninfected ones.”
#SLE #Bcell #EBV
“These EBV-infected B cells with antigen-presenting abilities had the capacity to activate autoreactive helper T cells, setting off a chain reaction where those T cells could activate other autoreactive B cells, including uninfected ones.”
#SLE #Bcell #EBV
Reposted by Landon Clark
In our latest study in @cp-immunity.bsky.social, we followed dynamics of vaccine-specific Tfh cells for 60+ weeks after immunization.
authors.elsevier.com/a/1m3mV3qNrU...
We captured >500,000 CD4 T cells including >36,000 vaccine-specific Tfh by scRNAseq, giving an unprecedented longitudinal detail.
authors.elsevier.com/a/1m3mV3qNrU...
We captured >500,000 CD4 T cells including >36,000 vaccine-specific Tfh by scRNAseq, giving an unprecedented longitudinal detail.
November 12, 2025 at 7:45 PM
In our latest study in @cp-immunity.bsky.social, we followed dynamics of vaccine-specific Tfh cells for 60+ weeks after immunization.
authors.elsevier.com/a/1m3mV3qNrU...
We captured >500,000 CD4 T cells including >36,000 vaccine-specific Tfh by scRNAseq, giving an unprecedented longitudinal detail.
authors.elsevier.com/a/1m3mV3qNrU...
We captured >500,000 CD4 T cells including >36,000 vaccine-specific Tfh by scRNAseq, giving an unprecedented longitudinal detail.
Reposted by Landon Clark
Epstein-Barr virus–mediated B cell reprogramming may initiate systemic lupus erythematosus
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/... @stanfordmedicine.bsky.social @shady-myounis.bsky.social
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/... @stanfordmedicine.bsky.social @shady-myounis.bsky.social
November 12, 2025 at 11:21 PM
Epstein-Barr virus–mediated B cell reprogramming may initiate systemic lupus erythematosus
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/... @stanfordmedicine.bsky.social @shady-myounis.bsky.social
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/... @stanfordmedicine.bsky.social @shady-myounis.bsky.social
Reposted by Landon Clark
Vaccine-specific Tfh undergo robust early expansion, sustained proliferation, months-long persistence w/o exhaustion, and functional specialization associated w/ enhanced germinal centers & humoral [email protected] @profshanecrotty.bsky.social
www.cell.com/immunity/ful... @lji.org
www.cell.com/immunity/ful... @lji.org
November 9, 2025 at 10:29 PM
Vaccine-specific Tfh undergo robust early expansion, sustained proliferation, months-long persistence w/o exhaustion, and functional specialization associated w/ enhanced germinal centers & humoral [email protected] @profshanecrotty.bsky.social
www.cell.com/immunity/ful... @lji.org
www.cell.com/immunity/ful... @lji.org
Reposted by Landon Clark
A small 🧵 encouraging people to publish protocols.
Our #AutoSpectral release has made me reflect on how much the community tends to appreciate protocol papers. People rarely present protocols as their main achievements, but they are often the most impactful work.
www.biorxiv.org/content/10.1...
Our #AutoSpectral release has made me reflect on how much the community tends to appreciate protocol papers. People rarely present protocols as their main achievements, but they are often the most impactful work.
www.biorxiv.org/content/10.1...
AutoSpectral improves spectral flow cytometry accuracy through optimised spectral unmixing and autofluorescence-matching at the cellular level
The advent of spectral flow cytometry has seen a rapid rise in the complexity of flow cytometry experiments, allowing the construction of assays with at least 50 fluorescent parameters. To correctly d...
www.biorxiv.org
October 31, 2025 at 9:09 AM
A small 🧵 encouraging people to publish protocols.
Our #AutoSpectral release has made me reflect on how much the community tends to appreciate protocol papers. People rarely present protocols as their main achievements, but they are often the most impactful work.
www.biorxiv.org/content/10.1...
Our #AutoSpectral release has made me reflect on how much the community tends to appreciate protocol papers. People rarely present protocols as their main achievements, but they are often the most impactful work.
www.biorxiv.org/content/10.1...
Reposted by Landon Clark
Pre-print alert! And this one really is a must read for anyone that does spectral #flowcytometry. It is a complete, fully-automated spectral unmixing pipeline that reduces error up to 9000-fold.
Want to get a tough sample, like lung, looking like this? Read more!
www.biorxiv.org/content/10.1...
Want to get a tough sample, like lung, looking like this? Read more!
www.biorxiv.org/content/10.1...
October 28, 2025 at 8:06 AM
Pre-print alert! And this one really is a must read for anyone that does spectral #flowcytometry. It is a complete, fully-automated spectral unmixing pipeline that reduces error up to 9000-fold.
Want to get a tough sample, like lung, looking like this? Read more!
www.biorxiv.org/content/10.1...
Want to get a tough sample, like lung, looking like this? Read more!
www.biorxiv.org/content/10.1...
Reposted by Landon Clark
Tertiary lymphoid structures in a subset of gliomas that show signs of functional adaptive immune activation and are associated with longer overall patient survival and an immune-permissive tumor microenvironment @cp-immunity.bsky.social
www.cell.com/immunity/ful...
www.cell.com/immunity/ful...
October 21, 2025 at 3:22 PM
Tertiary lymphoid structures in a subset of gliomas that show signs of functional adaptive immune activation and are associated with longer overall patient survival and an immune-permissive tumor microenvironment @cp-immunity.bsky.social
www.cell.com/immunity/ful...
www.cell.com/immunity/ful...
Reposted by Landon Clark
@biorxivpreprint.bsky.social Tertiary lymphoid structures support the development of allergen-specific progenitor CD4+ T cells @marionpepper.bsky.social
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
October 7, 2025 at 3:02 PM
@biorxivpreprint.bsky.social Tertiary lymphoid structures support the development of allergen-specific progenitor CD4+ T cells @marionpepper.bsky.social
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Landon Clark
Pre-division TCF1 drop determines long-term CD8 T cell fates @biorxivpreprint.bsky.social
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
October 7, 2025 at 3:21 PM
Pre-division TCF1 drop determines long-term CD8 T cell fates @biorxivpreprint.bsky.social
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Landon Clark
A small primer on the #NobelPrize awarded to Mary E. Brunkow, Fred Ramsdell and Shimon Sakaguchi today. This prize was for combining two separate fields of immunology research - genetic research on IPEX and immunology research of regulatory T cells (#Tregs), with enormous impact on biology/medicine
October 6, 2025 at 11:21 AM
A small primer on the #NobelPrize awarded to Mary E. Brunkow, Fred Ramsdell and Shimon Sakaguchi today. This prize was for combining two separate fields of immunology research - genetic research on IPEX and immunology research of regulatory T cells (#Tregs), with enormous impact on biology/medicine
Reposted by Landon Clark
Germinal centers can override peripheral tolerance, recruiting previously silent autoreactive clones and facilitating diversification of autoantibodies @jimmunol.bsky.social @harvardmed.bsky.social
academic.oup.com/jimmunol/adv...
academic.oup.com/jimmunol/adv...
October 3, 2025 at 3:52 AM
Germinal centers can override peripheral tolerance, recruiting previously silent autoreactive clones and facilitating diversification of autoantibodies @jimmunol.bsky.social @harvardmed.bsky.social
academic.oup.com/jimmunol/adv...
academic.oup.com/jimmunol/adv...
Reposted by Landon Clark
Divergent cytokine and transcriptional signatures control functional T follicular helper cell heterogeneity @natimmunol.nature.com @groomlab.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
September 9, 2025 at 5:59 PM
Divergent cytokine and transcriptional signatures control functional T follicular helper cell heterogeneity @natimmunol.nature.com @groomlab.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Landon Clark
CD11c+ Tbet+ B cells constrain obesity- and vaccination-induced germinal center B cells and T helper cells @biorxivpreprint.bsky.social @eleadbetter.bsky.social
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
September 6, 2025 at 12:46 AM
CD11c+ Tbet+ B cells constrain obesity- and vaccination-induced germinal center B cells and T helper cells @biorxivpreprint.bsky.social @eleadbetter.bsky.social
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...