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Tal Laviv @lavivtal.bsky.social · Feb 21

Thanks Kasia!

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Tal Laviv @lavivtal.bsky.social · Feb 21

Thanks Vidhya!

Tal Laviv @lavivtal.bsky.social · Feb 21

Thanks Hyungbae!

Tal Laviv @lavivtal.bsky.social · Feb 20

Thanks!

Tal Laviv @lavivtal.bsky.social · Feb 20

Thanks so much Tommaso!

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Tal Laviv @lavivtal.bsky.social · Feb 20

Thanks!

Tal Laviv @lavivtal.bsky.social · Feb 20

Thanks Nicolas!

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Tal Laviv @lavivtal.bsky.social · Feb 20

Thanks so much!!

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Tal Laviv @lavivtal.bsky.social · Feb 20

Thank you!

Tal Laviv @lavivtal.bsky.social · Feb 20

Thanks Rita!

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Tal Laviv @lavivtal.bsky.social · Feb 20

Thanks Jonas!!

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Tal Laviv @lavivtal.bsky.social · Feb 20

Thanks Onur!

Tal Laviv @lavivtal.bsky.social · Feb 20

This project was spearheaded by a Tomar Kagan, a talented PhD student in my lab. special thanks for @ritastrack.bsky.social and nature methods team for their professional and supportive process to improve the paper to its present form, it was a real pleasure to work with them.

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Tal Laviv @lavivtal.bsky.social · Feb 20

This highlights the need to explore PTEN signaling in a cell-specific manner, and to further explore the original findings and associate PTEN signaling during early brain development! I would like to thank all the collaborators that helped us in this project, and my lab members for their hard work.

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Tal Laviv @lavivtal.bsky.social · Feb 20

I will end with our favorite and perhaps most exciting finding. We used a red shifted PTEN sensor, and combined it to a dual color PTEN imaging in excitatory and Inhibitory cells. We found differential experience dependent changes in E and I cells!

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Tal Laviv @lavivtal.bsky.social · Feb 20

But how about interfering with PTEN function? For this we used a LOF mutation previously identified in a PTEN screen. This manipulation still maintains flexible conformational change of PTEN but completely mitigates the effect on downstream signaling and cellular structure and function.

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Tal Laviv @lavivtal.bsky.social · Feb 20

Luckily for us, nature already made PTEN an ideal substrate- since it undergoes a conformational transition upon activation, from closed to open state. We use this to tag PTEN with FP and dynamically monitor PTEN conformation a proxy for its activity state.

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Tal Laviv @lavivtal.bsky.social · Feb 20

Despite this important role, there were no tools to directly measure PTEN activity, in particular in single cell resolution and in intact neuronal circuits. We engineered a FRET based PTEN sensor, which is optimized for 2pFLIM.

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Tal Laviv @lavivtal.bsky.social · Feb 20

Numerus human cancers are associate with PTEN mutations. In the brain, mutations in PTEN lead to epilepsy, autism and macrocephaly.
Work in mouse models showed that both an increase and a decrease in PTEN levels has detrimental neuronal consequences.

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Tal Laviv @lavivtal.bsky.social · Feb 20

We have developed a biosensor to monitor PTEN activity in living cells, tissue and even whole organisms!! PTEN is a vital phosphatase found in every cell, and acts as an inhibitor of cellular tropism, essential to protect cells from excessive growth and proliferation.

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Tal Laviv @lavivtal.bsky.social · Feb 20

I started my lab more then 4 years ago, and we have endured some very challenging times ever since.…I am very excited to share the first paper from my lab, published now in @naturemethods.bsky.social
www.nature.com/articles/s41...

Genetically encoded biosensor for fluorescence lifetime imaging of PTEN dynamics in the intact brain - Nature Methods

A first-in-class fluorescence lifetime-based FRET sensor for phosphatase and tensin homolog (PTEN) enables the dynamic monitoring of PTEN activity in cultured cells and in vivo with high spatiotempora...

www.nature.com

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Reposted by Tal Laviv

Inna Slutsky @innaslutsky.bsky.social · Jan 15

Yesterday, we held the 2024 Sieratzki Prizes Ceremony at the ISFN meeting in Eilat. We are so proud of our 2024 winners: @yoavlivneh.bsky.social , Arseny Finkelstein, David Omer, @lavivtal.bsky.social, and Inbal Ben-Ami Bartal!

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Reposted by Tal Laviv

Eric Topol @erictopol.bsky.social · Dec 19

How to improve memory and cognitive function in aged mice
Fix the mitochondrial EC (excitation-coupling)
@science.org
representing a new potential path for replenishing neuronal energy reserves to counter brain aging
www.science.org/doi/10.1126/...

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Tal Laviv @lavivtal.bsky.social · Dec 20

Beautiful work by my the lab of Huan Ma, which I had the pleasure to contribute to. A fascinating link between neuronal activity, and transcription-excitation coupling in mitochodria to brain function and ageing www.science.org/doi/10.1126/...

Boosting neuronal activity-driven mitochondrial DNA transcription improves cognition in aged mice

Deciphering the complex interplay between neuronal activity and mitochondrial function is pivotal in understanding brain aging, a multifaceted process marked by declines in synaptic function and mitoc...

www.science.org

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Tal Laviv @lavivtal.bsky.social · Dec 13

Congratulations!!!

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