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Luke Gamon

@lgamon.bsky.social

700 followers 300 following 340 posts

All things teammassspec/realtimechem. Survives on only the fanciest coffee and beer. Laser capture microdissection | spatial proteomics | protein modification | atherosclerosis | inflammation and oxidation

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Luke Gamon @lgamon.bsky.social · Jun 23

Pumped to finally show off our spatial proteomics work presented a few weeks ago at the EAS Congress @eassociety.bsky.social and now live on @biorxivpreprint.bsky.social 🥳

Time for a science thread! #TeamMassSpec #SpatialProteomics #AtheroSky 🧵

www.biorxiv.org/content/10.1...

www.biorxiv.org

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Luke Gamon @lgamon.bsky.social · 1d

Upvote for this one. It’s mainly just a little annoying that it’s not in the output file directly but not ‘so’ difficult to write a script to do it yourself.

Reposted by Luke Gamon

Ana Martínez del Val @anamdv.bsky.social · 2d

Do you have an exciting experiment that you'd love to do using proteomics in the context of your work? YPIC funds 5000 eur for your research idea to come to life!

⬇️⬇️⬇️⬇️⬇️

ypic.bsky.social @ypic.bsky.social · 15d

🎉 Exciting opportunity for students in proteomics! 🎉
The Student Proteomics Fund (SPF) is back!

📅 Key dates:
Call opens: October 1, 2025
Submission deadline: November 30, 2025
Don’t miss your chance to bring your research idea to life!
New boost
#SPF #YPIC #Proteomics

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Reposted by Luke Gamon

Cancer Research UK Manchester Institute @cruk-mi.bsky.social · 5d

Finally, we're looking for someone to join our #MassSpec Core Facility to support all the protein/peptide MS & development needs of our research groups utilising:

🧪Protein chemistry
📈Liquid chromatography
⚛️Tandem mass spectrometry
🖥️Data analysis

More at:
www.cruk.manchester.ac.uk/career/mi-25...

Mass Spec

xkcd.com

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Luke Gamon @lgamon.bsky.social · 4d

Definite flashbacks to my PhD days. If there weren’t chunks settling in the bottom you know you’d have to book an overnight 13C. Ain’t nobody got time for that 😇

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Luke Gamon @lgamon.bsky.social · 6d

Join a department that gives you surprise slushies for the yearly department seminar day.

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Luke Gamon @lgamon.bsky.social · 7d

4th grant application of the year sent out into the ether. Number 5 will leave the coop next week.🤞

Reposted by Luke Gamon

Arne Elofsson @bioinfo.se · 8d

Come work with me. Excellent startup package. su.varbi.com/en/what:job/...

Assistant Professor in Data-driven cell and molecular biology

At Stockholm University the Department of Biochemistry and Biophysics (DBB) and the Department of Molecular Biosciences, The Wenner-Gren Institute (MBW) are looking to fill one position as DDLS fellow

su.varbi.com

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Luke Gamon @lgamon.bsky.social · 11d

I’m seeing some chatter about a new centralised ID system in the UK.

As someone who lives in a country where basically your entire identity is digitally linked to a single ID I have mixed feelings but damnnnnn is life smooth when everything is integrated.

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Luke Gamon @lgamon.bsky.social · 13d

Is it that expensive? Can you dilute it? Don’t need that much signal, at least for a calibration?

Luke Gamon @lgamon.bsky.social · 16d

Sodium formate doesn’t quite get to the same m/z range for proteomics with the usual MS settings, so yes, not ideal. There must be something you can use… even a mix of PEG and sodium formate 😅?

Luke Gamon @lgamon.bsky.social · 16d

Sodium formate is probably the cheapest option. Great for low m/z especially.

Luke Gamon @lgamon.bsky.social · 19d

We typically run at equivalent of 60-100 SPD with ~80% instrument utilisation (ie 80% of the time is spent analysing peptides, 20% equilibration/washing of the analytical column). Very happy with it.

Luke Gamon @lgamon.bsky.social · 20d

DTSC is the answer? 😇

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Reposted by Luke Gamon

Michael MacCoss @maccoss.bsky.social · 23d

Proteomics Webinar: DIA with FragPipe, DIA-NN, and Skyline
Presenters: Eduard Sabidó and Brendan MacLean
When: Tuesday, September 16, 8am (Pacific Time)
Register Now ... skyline.ms/project/home...

#massspec #proteomics

Start Page: /home/software/Skyline/events/2025 Webinars/Webinar 26

skyline.ms

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Luke Gamon @lgamon.bsky.social · 25d

Our new spatial proteomics workflow is a game changer. Prepped 40 samples on Friday in 6hrs all the way from tissue on a slide to peptides in the autosampler.

Almost reminds me of my organic chem PhD days when you could think up an experiment in the morning and have the result by the afternoon.

Luke Gamon @lgamon.bsky.social · 26d

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Luke Gamon @lgamon.bsky.social · 26d

In the end it turned out the PTM we thought we had was additionally cleaved by trypsin. Could never find it in a standard search and hadn’t thought hard enough to realise it might be post-processed (it had a peptide like amide bond)

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Luke Gamon @lgamon.bsky.social · 26d

If you have a +/- PTM sample comparison I you could try a trick that worked wonders for me. Put the mzml’s through XCMSonline to identify ions that were differentially abundant across the gradient, then ran the samples multiple times with different collision energies to improve b/y ion coverage.

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Luke Gamon @lgamon.bsky.social · 26d

A little early morning science with the 2.5yo. Always been obsessed with gloves for some reason? Probably even better with PPE than I am

Luke Gamon @lgamon.bsky.social · 27d

Congrats Frederik!! Such nice work and especially stunning images 👌

Reposted by Luke Gamon

CNIC Proteomics Lab @cnicproteomics.bsky.social · 27d

@cnicproteomics.bsky.social is finally in Bluesky!!

Follow us to learn about our quest to advance proteomics research with a special focus on open search strategies and the study of post-translational modifications (PTMs).

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Reposted by Luke Gamon

Lasse Bach Steffensen @lasse-bs.bsky.social · Sep 6

📢 My lab has a 2.5-year Postdoc position available!

If you're interested in smooth muscle cell biology in atherosclerosis, then check out the Postdoc call here:

fa-eosd-saasfaprod1.fa.ocs.oraclecloud.com/hcmUI/Candid...

#postdoc #research #hiring #atherosclerosis

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Reposted by Luke Gamon

Lindsay K Pino @lindsaykpino.com · Sep 6

Do you or your lab use the Skyline software for #MassSpectrometry #proteomics? I'm looking for instructors to help with this year's Skyline Online, a virtual workshop/crash-course for all things Skyline! I'm especially looking for early career researchers for this opportunity. Please DM!

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Luke Gamon @lgamon.bsky.social · Sep 7

First author is a close friend BTW. He was my first PhD student!