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Reposted by Lennard Ostendorf

Dimitrios L. Wagner @dlwagner.bsky.social · 19d

You like engineering T cells, but you are missing the right flavor?

Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1

We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪

Versatile and efficient non-viral integration of large transgenes in human T cells via CRISPR knock-in and engineered integrases

Current gene transfer methods often lack the precision, versatility, or efficiency when integrating large transgenes, limiting the ability to engineer therapeutic T-cells with more complex payloads. H...

www.biorxiv.org

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c0nc0rdance @c0nc0rdance.bsky.social · Aug 30

A conference of three primates, discussing videography and their varying perspectives on nature documentaries.

Jane Goodall and husband Hugo van Lawick, 1974.
Third primate's name unknown.

Jane Goodall and husband Hugo van Lawick share a moment with a monkey in this January 1974 photo. It is crawling up a tripod to inspect the lens of the camera while the two humans look on.

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Reposted by Lennard Ostendorf

Los Angeles Times @latimes.com · 7d

Jane Goodall, the trailblazing naturalist whose intimate observations of chimpanzees in the African wild produced powerful insights that transformed basic conceptions of humankind, has died. She was 91.

Read more about her legacy www.latimes.com/obituaries/s...

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Lennard Ostendorf @lostendorf.bsky.social · 11d

Really enjoy your threads, thank you! 🔥

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Lori O'Brien @llobrienlab.bsky.social · 12d

For this #FluorescenceFriday, a gorgeous image of an adult mouse kidney labeled with AQP2 and alpha SMA antibodies. AQP2 (green) marks the collecting duct and distal connecting segment while SMA marks the arterial tree. Courtesy of talented postdoc Sarah McLarnon.

Image of a fluorescently labeled adult mouse kidney showing AQP2 staining of collecting ducts and connecting segment in green and alpha SMA staining of the arterial tree in magenta. The collecting ducts look like squiggly branches.

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Lennard Ostendorf @lostendorf.bsky.social · 12d

Rarely is a taped live performance so much better than the original. Love this Maribou State recording start to finish 🔥
Great for doing annoying point and click data analysis today.

www.youtube.com/watch?v=oQjg2QEvEX0

Maribou State Live From Alexandra Palace, London

YouTube video by DJ Mag

www.youtube.com

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Reposted by Lennard Ostendorf

Adrian Liston @labliston.bsky.social · 19d

For our #flowcytometry peeps, would you like to have a single fix/perm protocol that is optimised for everything? One that preserves fluorophores while allowing simultaneous TF and cytokine staining? How about 100-fold cheaper?

You got it:
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...

currentprotocols.onlinelibrary.wiley.com

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Lennard Ostendorf @lostendorf.bsky.social · 24d

So proud of these guys. Amazing final game, the turkish team played impressive basketball, but in the end we prevailed 🔥🏀

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immunoboys @immunoboys.bsky.social · 28d

Plasmacytoid dendritic cells can transition to become cDC2s under to influence of TNF, while type-I interferon reduces TNFR expression and thereby inhibits transition. These transitioned cDC2 seem fully functional. 🧪 ⑂

Hornero et al 2025 in Nat Immunol
www.nature.com/articles/s41...

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immunoboys @immunoboys.bsky.social · Sep 6

Horisberger et al separate Lupus nephritis patients in 3 groups by blood:
healthy-like, interferon-dominant and CD8 T cell-dominant.
The CD8 T cell-dominant group had the highest biopsy activity score and highest response rate after 1 year; the healthy-like the most chronic damage. 🧪 ⑂ #NephSky
1/

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Lennard Ostendorf @lostendorf.bsky.social · Sep 4

Nice, Herzlichen Glückwunsch! 🔥

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Adrian Liston @labliston.bsky.social · Sep 3

🧵How to write and manage your first research budgets

The point of funding is to convert it into quality research. A well-spent research budget should fund the idea it was raised on, plus revision experiments, plus preliminary data for the next grant. So you need to spend, while avoiding waste.

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Lennard Ostendorf @lostendorf.bsky.social · Sep 3

Was ne Mannschaft! 🔥🏀

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Reposted by Lennard Ostendorf

Ivan Zanoni @lozanzi.bsky.social · Sep 3

Finally officially out in the latest issue of @cp-cell.bsky.social as a #free #featured #LeadingEdge paper: everything you wanted to know about #InterferonPower! ❤️ @danielboehmer.bsky.social @bostonchildrens.bsky.social @harvardmed.bsky.social www.cell.com/cell/fulltex...

Interferons in health and disease

The cytokine messenger proteins known as interferons are central to protective immune responses against infections, but they are also involved in inflammatory and autoimmune diseases. This review maps...

www.cell.com

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Lennard Ostendorf @lostendorf.bsky.social · Sep 2

Such a neat story – I learned that this happens because mitochondria have their own set of tRNAs with only one per amino acid.

Congratulations Patrick, @leifludwig.bsky.social and everyone involved!

immunoboys @immunoboys.bsky.social · Sep 2

A synonymous mitochondrial variant is negatively selected against in short-lived CD8 effectors – How?
The variant changes tRNA pairing from Watson-Crick-Franklin to wobble, thereby reducing translational efficiency in high energy demand. 🧪 ⑂
Lareau et al – 2025
www.pnas.org/doi/10.1073/...

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Lennard Ostendorf @lostendorf.bsky.social · Aug 28

I think there is a difference here between clinical papers (where I mostly agree) and more basic science/translational research (where I enjoy reading the introduction/discussion).

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Reposted by Lennard Ostendorf

Mark A. Hanson @hansonmark.bsky.social · Aug 14

A reminder you/your lab can support FlyBase at Cambridge through the following link. Every bit helps. Please share if you yourself can't donate.

www.philanthropy.cam.ac.uk/give-to-camb...

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immunoboys @immunoboys.bsky.social · Aug 10

Effector CD8 T cells have punctate mitochondria with loose cristae, favoring glycolysis and memory T cells elongated mitochondria with tight cristae, favoring OxPhos.
Pharmacologic remodeling of mitochondria improves Teffs in adoptive transfer. 🧪 ⑂

Buck et al 2016 doi.org/10.1016/j.ce...

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Lennard Ostendorf @lostendorf.bsky.social · Aug 7

In Lupus nephritis patients, neutrophils have increased surface LAMP1, which is externalized after activation. Soluble LAMP1 is increased both in the blood and urine of patients, correlating with histological activity. #NephSky 🧪
Our paper in @eurjimmunol.bsky.social
doi.org/10.1002/eji....

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immunoboys @immunoboys.bsky.social · Jul 30

Resting MAIT cells generally express the non-cytotoxic Granzyme K, but rapidly switch to the cytotoxic Granzyme B upon stimulation. Consequently, previously stimulated MAITs kill much more target cells than resting ones. 🧪

Kurioka et al – 2015 – @mucosalimmunol.bsky.social
doi.org/10.1038/mi.2...

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Lennard Ostendorf @lostendorf.bsky.social · Jul 27

What a crazy 🔥 arrangement:
www.youtube.com/watch?v=1Qp4...

Pitch, Please! - Flesh & Bone

YouTube video by Pitch, Please! A Cappella

www.youtube.com

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Lennard Ostendorf @lostendorf.bsky.social · Jul 23

For me, that's called a cafè. Love to do writing work with some background noise.

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immunoboys @immunoboys.bsky.social · Jul 20

MR1-presented cholic acid 7-sulfate + 3-sulfate are endogenous stimulatory ligands for MAIT cells. Stimulation is much weaker than by traditional ligand 5-OP-RU, potentially contributing to steady state activation and thymic generation of MAITs.

Ito et al 2024
www.science.org/doi/10.1126/...

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Emily Moin @emilymoin.com · Jul 15

Tastes like calculating the serum osm gap

Cabel Sasser @cabel.panic.com · Jul 12

i’m not a drinker so i was unaware that we had invented BIG SIPZ JUGZ, a 16% wine cocktail that looks like a cute little poison milk jug and includes a “my name is” label on the side to break the party ice and is, yes, ‘MERICA flavored.

maybe i should start drinking after all. what could go wrong

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Reposted by Lennard Ostendorf

Nephrology Journal Club @nephjc.bsky.social · Jul 16

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Last #NephJC we discussed the combination therapy of empagliflozin and finerenone 💊 #TenPostNephJC

🌵From a therapeutic dessert to a flood of options in diabetic kidney disease (DKD) - what is the best timing to keep the kidneys afloat

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