very much in the spirit of sharing something small, a portion of my own opinion on LLPS is here. I offer it only as one view among many, with sincere respect for others who think differently and with openness to further discussion, clarification, or correction of emphasis.

doi.org/10.5281/zeno...

If lipid raft supporters would please engage meaningfully with these critiques in their recent publications, I would no longer feel compelled to debate them outside of print.

I admit that I have taken advantage of your threads to attract attention to the lipid raft issue in membrane biology, which in modified forms, often use LLPS terminology. This is because they do not respond to me otherwise.

doi.org/10.5281/zeno...

www.researchgate.net/publication/...

Yes, if properly defined I think it could absolutely be falsifiable. Apologies, this debate got heated so I deleted most of my replies to avoid more mayhem.

Apologies to anyone seeing this: it got ugly. Definitely not the proudest moment for both of us.

I have read some but not all your work. My understanding now is that you are using a personalized definition for phase separation, which is the right thing to do. Could you post it here? Please don't make this personal: I'm not attacking your work; I just don't like pseudoscience.

Oh, so we weren't on the same page. Then let me spell it out: equilibrium is a precondition for canonical phase separation.

You misinterpreted again: I asked for the opposite of this; for you to show me a falsifiable claim demonstrating the phase separation is scientific.

You misinterpret: I want you show me a falsifiable claim that demonstrates phase separation is scientific

Excellent start. How much is the temperature shift? Aren't all cells in hysteresis?

As always: I demand that you present a falsifiable claim. Absent of any, there is no scientific substance.

Sorry, what are you referring to? I do not see which comment this one is attached to. What words am I putting in your mouth?

I demand falsifiability. Tell me specifically what I can and cannot call phase separation.

Please also include this one in your journal club, at least the lipid raft section:

Kervin, T.A., Mangalam, M. Lessons from pseudoscience in biology. (2025). www.researchgate.net/publication/...

Having not read this yet: could they not have just been akin to the typical machinery that creates membranes zones without trafficking? I mean, assuming they bind lipids well

Agreed: nice and testable. Not an unfalsifiable metaphor.

I disagree, a scientific theory must be falsifiable. Phase separation, equilibrium, etc. are just concepts, not theories. Who is to say whether they are true or false if they cannot be falsified?

Precisely; it's philosophy. The basic definition requires detailed balance, which is a concept that does not exist in the physical world. Instead, detailed balance, etc. are analytical concepts, not scientific theories.