Mike Tisza
@miketisza.bsky.social
90 followers 91 following 15 posts
In a sequence gaze daze Bfx in HTX 🧬🖥️ Opinions my own 🦐 https://tiszalab.github.io/
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Reposted by Mike Tisza
karatasmustafa.bsky.social
New paper out!

We sampled indoor air for a year in a Belgian daycare and used shotgun metagenomics to track viruses. We recovered many viral genomes of interest.

What would you sample next?

Read at @eurosurveillance.org
@emmanuel-microb.bsky.social, @jellematthijnssens.bsky.social
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Figure 3. (A–N) Sequencing coverage plots of genetic sequences of viruses detected in indoor air samples of a daycare and phylogenetic analyses of sequences of (O) two human bocaviruses and (P) one RSV identified in some samples, Belgium, January–December 2022
Reposted by Mike Tisza
medrxivpreprint.bsky.social
Wastewater Sequencing Reveals Persistent Circulation and Rising Prevalence of Several Oncogenic Viruses Across Texas https://www.medrxiv.org/content/10.1101/2025.09.17.25335998v1
Reposted by Mike Tisza
bioinf.bsky.social
How do you long-read sequence metagenomes? I would argue it starts with the right sample storage & DNA extraction, to enable efficient @nanoporetech.com /@pacbio.bsky.social sequencing, which we investigated in our new paper: www.biorxiv.org/content/10.1...

Massive thanks to Klara for driving this
Reposted by Mike Tisza
jimshaw.bsky.social
Preprint out for myloasm, our new nanopore / HiFi metagenome assembler!

Nanopore's getting accurate, but

1. Can this lead to better metagenome assemblies?
2. How, algorithmically, to leverage them?

with co-author Max Marin @mgmarin.bsky.social, supervised by Heng Li @lh3lh3.bsky.social

1 / N
biorxiv-bioinfo.bsky.social
High-resolution metagenome assembly for modern long reads with myloasm https://www.biorxiv.org/content/10.1101/2025.09.05.674543v1
Reposted by Mike Tisza
apcamargo.bsky.social
A BLAST update adding support for compressed files and csv output with headers is a Good Friday night surprise!

blast.ncbi.nlm.nih.gov/doc/blast-ne...
2025 BLAST NEWS — BlastNews 0.1.1 documentation
blast.ncbi.nlm.nih.gov
miketisza.bsky.social
I want to thank my excellent co-authors and collaborators for the fantastic teamwork. If you want to test Cenote-Taker 3 yourself, please see the installation instructions on the GitHub repo: github.com/mtisza1/Ceno...
GitHub - mtisza1/Cenote-Taker3: Discover and annotate the virome
Discover and annotate the virome. Contribute to mtisza1/Cenote-Taker3 development by creating an account on GitHub.
github.com
miketisza.bsky.social
I'm very excited to share our manuscript for Cenote-Taker 3. We show that (1) CT3 quickly and accurately annotates previously uncatalogued virus genomes and (2) finds divergent virus genomes in contiguous assemblies. Virome benchmarks are SO hard, but I think we came up with clever and useful ones.
Cenote-Taker 3 benchmarks
miketisza.bsky.social
Thanks so much, Ludo! Again, these both look great, and i'm hoping to utilize them soon.
Reposted by Mike Tisza
viromegirl.bsky.social
Do you love phage? I have an opening for a postdoc in my lab at the University of South Florida College of Marine Science (@cmarinescience.bsky.social) looking at interactions between marine phage and iron, which is an important limiting trace metal in the oceans (1/5) 🧵#phagesky 🦠🌊
Electron micrograph of two phage infecting a host cell side-by-side, with hearts between them
Reposted by Mike Tisza
caleblareau.bsky.social
Excited to share a new preprint from the lab with @ryandhindsa.bsky.social ! www.biorxiv.org/content/10.1...

Led by @sherrynyeo.bsky.social, @erinmayc.bsky.social, and friends, we continue our journey to find viral DNA in our favorite place-- the overlooked and discarded reads in existing data! 1/
the treasure trove of all sequencing datasets
Reposted by Mike Tisza
simrouxvirus.bsky.social
New pre-print out \o/ All about CRISPR, metagenomes, and what you learn when you collect (a lot of) spacers from natural communities, with @apcamargo.bsky.social @urineri.bsky.social @lhug.bsky.social but also Uri Gophna, Nikhil George (not on Bsky I think) & others at JGI doi.org/10.1101/2025...
doi.org
Reposted by Mike Tisza
evelienadri.bsky.social
Join my lab as a postdoctoral research scientist to investigate the diversity of drug-resistant #Salmonella and how #bacteriophages can help future food safety.

Apply by 2 July, details below!
quadraminstitute.bsky.social
🆕 Vacancy! We’re looking for a Research Scientist to join Dr @evelienadri.bsky.social‬ 's group to study bacteriophages, drug resistance and genetic evolution of Salmonella 🦠

💷 £36,720 to £39,750
🗓️ Apply by 2 July 2025
➡️ buff.ly/7M2mDaa
A social card with an illustration of a bacteriophage and bacteria and the text "Research Scientist to join Dr Evelien Adrianssen's group to study bacteriophage, drug resistance and genetic evolution of Salmonella, Salary: 36,720 to 39,750, Contract length: 1.5 years, Apply by 2 July 2025
Reposted by Mike Tisza
daanspeth.bsky.social
I'm happy to announce the latest release of the GlobDB, available at globdb.org.

The GlobDB is a database of "species dereplicated" microbial genomes, and as of release 226 contains twice the number of species-representative genomes (306,260) than the latest GTDB release.
home | GlobDB
globdb.org
Reposted by Mike Tisza
jimshaw.bsky.social
Announcing myloasm, a new long-read (ONT R10/PacBio) metagenome assembler that I've been working on during my postdoc in the Heng Li lab (@lh3lh3.bsky.social).

myloasm-docs.github.io
myloasm - metagenomic assembly with (noisy) long reads
myloasm-docs.github.io
Reposted by Mike Tisza
bcmfromthelabs.bsky.social
An innovative outbreak detection program that tracks viruses in #wastewater identified the #measles virus in #Houston in early January 2025, before cases were reported. #TailorLabs #saracregeen @miketisza.bsky.social @bcmhouston.bsky.social #TEPHITexas #RiceU @apha.org www.bcm.edu/news/measles...
Reposted by Mike Tisza
tailor-labs.bsky.social
Another example of how wastewater sequencing is going to positively impact Public Health. TEPHI's Outbreak Detection program shows once more the sensitivity and specificity of agnostic sequencing .... ajph.aphapublications.org/doi/abs/10.2...
Reposted by Mike Tisza
jcamthrash.bsky.social
Recombination and the Species Structure of the Genus Bacteroides www.biorxiv.org/content/10.1... #jcampubs
Reposted by Mike Tisza
karatasmustafa.bsky.social
🎉🎉 Happy to share our new paper! 🎉🎉

Have you ever wondered about the animal viruses living right alongside us in our cities?

Between our pets at home, rats in the sewers, pigeons on the streets, insects everywhere, and even farm animals nearby, it’s no surprise +++
Reposted by Mike Tisza
benadler.bsky.social
🧪🧪🧪10 incredible findings about Cas10-relative, mCpol:
result number 10 will surprise you!🧪🧪🧪

@erinedoherty.bsky.social and I teamed up to understand the role and function of Cas10-relative, mCpol, and its role in antiphage immunity. For more, check out Erin's thread 👇
erinedoherty.bsky.social
Preprint alert! ✨ In this project that I co-led with @benadler.bsky.social, we show that a miniature CRISPR-Cas10-like enzyme, mCpol, uses a novel inverse signaling mechanism to prevent the spread of viruses that attempt immune evasion by depleting host cyclic nucleotides.

Check it out:
A miniature CRISPR-Cas10 enzyme confers immunity by an inverse signaling pathway
Microbial and viral co-evolution has created immunity mechanisms involving oligonucleotide signaling that share mechanistic features with human anti-viral systems. In these pathways, including CBASS a...
www.biorxiv.org
Reposted by Mike Tisza
biorxiv-microbiol.bsky.social
Comparative wastewater virome analysis with different enrichment methods https://www.biorxiv.org/content/10.1101/2025.03.25.645222v1
Reposted by Mike Tisza
karatasmustafa.bsky.social
📢 Excited to share the first paper of my PhD: 14 years of rotavirus epidemiology in Belgium!

Read the full story on Eurosurveillance: @eurosurveillance.org

👉 www.eurosurveillance.org/content/10.2...

#Rotavirus #Virology #PublicHealth #Epidemiology #EpiSky #IDsky

Key messages -->
eurosurveillance.org
To answer their study question on the influence of the COVID-19 pandemic on rotavirus infections in Belgium, @karatasmustafa.bsky.social et al. used data collected 10 years before, 2 years during, and 2 years after the pandemic

#IDsky #EpiSky 🦠

👇🏽
jellematthijnssens.bsky.social
🚨 New paper alert! 🚨 How did the #COVID_19 pandemic impact #rotavirus epidemiology in Belgium? Read all about it in our latest paper in @Eurosurveillanc:
eurosurveillance.org/content/10.2...
@karatasmustafa.bsky.social @vanranstmarc.bsky.social @wollants.bsky.social
Reposted by Mike Tisza
evelienadri.bsky.social
🎓 #PhDPosition available in my group!

🫁 Understanding the microbiome and phageome in chronic lung infections for the development of phage therapy with me & Prof Eleanor Mishra

📅 Apply by Monday 14 April

➡️ buff.ly/7v9JVDU

Happy to answer questions, but don't send your CV through email.
Illustrations of the microbiome including bacteriophages with the text "Funded PhD, understanding the microbiome and phageome in chronic lung infections for the development of phage therapy with Dr Evelien Adriaenssens and Professor Eleanor Mishra. Apply by 14 April 2025" Logos of the Quadram Institute and Microbes Microbiomes Bioinformatics Doctoral Training Partnership
Reposted by Mike Tisza
richardsever.bsky.social
Big news: we are setting up a new non-profit organization to run bioRxiv and medRxiv. It's called openRxiv [no it's not a new preprint server; it's dedicated organization to oversee the servers] openrxiv.org 1/n
Homepage - openRxiv
openRxiv is an independent non-profit, the new organizational home for bioRxiv and medRxiv, enabling researchers to instantly share groundbreaking findings with the global scientific community.
openrxiv.org