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Miro Astore

@miroastore.bsky.social

420 followers 510 following 20 posts

Flatiron Research Fellow @flatironinst. Studying temperature sensing ion channels with molecular dynamics and cryoEM❄️🔬. @sydney_physics allum. he/him

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Miro Astore @miroastore.bsky.social · 17d

Phew!

Miro Astore @miroastore.bsky.social · 18d

The text of the order says it's a one time charge for new petitions, renewals and transfers.

www.whitehouse.gov/presidential...

Restriction on Entry of Certain Nonimmigrant Workers

BY THE PRESIDENT OF THE UNITED STATES OF AMERICA A PROCLAMATION The H-1B nonimmigrant visa program was created to bring temporary workers into the United

www.whitehouse.gov

Reposted by Miro Astore

Oli Clarke @olibclarke.bsky.social · 26d

This one is a bit of a departure from the usual and definitely a work in progress!

We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.

Read on for details... 1/x

Miro Astore @miroastore.bsky.social · 24d

I'm very excited to announce that I've just joined Steve_Bonilla's lab at @rockefelleruniv.bsky.social. With the help of @pilarcossio.bsky.social and colleagues at @flatironinstitute.org. I'll be developing cryoEM into a quantitative method to study RNA biophysics.

Reposted by Miro Astore

Khaled_Selim Lab @selimlab.bsky.social · Jul 24

The results of the first #cryoEM heterogeneity challenge are up on biorxiv!
www.biorxiv.org/content/10.1...

Miro Astore @miroastore.bsky.social · Jul 24

Link that works
www.biorxiv.org/content/10.1...

The Inaugural Flatiron Institute Cryo-EM Conformational Heterogeneity Challenge

Despite the rise of single particle cryo-electron microscopy (cryo-EM) as a premier method for resolving macromolecular structures at atomic resolution, methods to address molecular heterogeneity in v...

www.biorxiv.org

Miro Astore @miroastore.bsky.social · Jul 23

Omg thank you. Oops

www.biorxiv.org/content/10.1...

The Inaugural Flatiron Institute Cryo-EM Conformational Heterogeneity Challenge

Despite the rise of single particle cryo-electron microscopy (cryo-EM) as a premier method for resolving macromolecular structures at atomic resolution, methods to address molecular heterogeneity in v...

www.biorxiv.org

Miro Astore @miroastore.bsky.social · Jul 23

Omg thank you. Oops

www.biorxiv.org/content/10.1...

The Inaugural Flatiron Institute Cryo-EM Conformational Heterogeneity Challenge

Despite the rise of single particle cryo-electron microscopy (cryo-EM) as a premier method for resolving macromolecular structures at atomic resolution, methods to address molecular heterogeneity in v...

www.biorxiv.org

Miro Astore @miroastore.bsky.social · Jul 23

You can also check out the analysis workflow and the datasets themselves if you'd like to learn more github.com/flatironinst...

www.simonsfoundation.org/flatiron/cen...

GitHub - flatironinstitute/Cryo-EM-Heterogeneity-Challenge-1: The Inaugural Flatiron Institute Cryo-EM Heterogeneity Community Challenge

The Inaugural Flatiron Institute Cryo-EM Heterogeneity Community Challenge - flatironinstitute/Cryo-EM-Heterogeneity-Challenge-1

Miro Astore @miroastore.bsky.social · Jul 23

Miro Astore @miroastore.bsky.social · Jul 23

This has been so much work from my coauthors Geoff Woollard and David Silva-Sanchez, mentors @sonyahanson.bsky.social
and @pilarcossio.bsky.social , Misha Kopylov at NYSBC, and all the participants who have continuously given us excellent feedback on the study and the manuscript. Thanks everybody!

Miro Astore @miroastore.bsky.social · Jul 23

The last metric we used calculates the likelihood that a given ensemble of volumes is contained in a set of images. This one is really exciting because we don't need any ground truth to compare to. We see the same trends between the simulated and experimental datasets.

Miro Astore @miroastore.bsky.social · Jul 23

The next is based on optimal transport. By calculating the differences between maps in a submission and the ground truth, we can calculate how close a submission is to the truth. We can also see how far from optimal participants estimated their populations.

Miro Astore @miroastore.bsky.social · Jul 23

Now comes the real work. Comparing all this amazing data! We came up with three novel ways of comparing series of volumes. With the first we simply perform PCA on the voxel maps and compare their bases. Turns out to be a super useful way of comparing motions between submissions.

Miro Astore @miroastore.bsky.social · Jul 23

We had amazing participation. Almost every method in the literature submitted to this challenge. We've chosen to keep the identity of the submissions anonymous (you'll see them in the manuscript denoted by icecream flavor hehe).

Miro Astore @miroastore.bsky.social · Jul 23

We asked each participant to give us two things. 80 volume maps which they thought gave a good representation of the molecular states in the data. And relative populations between those molecular states.

Miro Astore @miroastore.bsky.social · Jul 23

That's why we launched this challenge. We made two datasets, one experimental and one simulated. The simulated one was made with structures from molecular dynamics simulations. Here we hid a triple Gaussian for participants to discover. (Movie credit David Herreros).

Miro Astore @miroastore.bsky.social · Jul 23

There has been an explosion of methods to determine molecular ensembles from single particle cryo-EM datasets. However, these methods all use totally different algorithms and it's currently unknown how to assess their accuracy without ground truth structures to compare with.

Miro Astore @miroastore.bsky.social · Jul 23

That's a wrap! The results of the first #cryoEM heterogeneity challenge are up on biorxiv!
biorxiv.org/content/10.110

Miro Astore @miroastore.bsky.social · Mar 30

Super excited for this one! Great job guys.

pilarcossio.bsky.social @pilarcossio.bsky.social · Mar 30

Check out our recent preprint 👇on counting particles to estimate populations in #cryoem: noise can bias the estimates; with Luke Evans & a great team.

bioRxiv Biophysics @biorxiv-biophys.bsky.social · Mar 29

Counting particles could give wrong probabilities in Cryo-Electron Microscopy https://www.biorxiv.org/content/10.1101/2025.03.27.644168v1

Reposted by Miro Astore

Lander Lab @landerlab.bsky.social · Mar 19

That’s a wrap on the 2025 Cold Spring Harbor CryoEM course! Huge thanks to the amazing students for your enthusiasm, the TAs for your dedication, and the invited lecturers for sharing your expertise. Keep pushing the boundaries of single particle EM - excited to see what you all accomplish next!

Miro Astore @miroastore.bsky.social · Mar 16

Watch out world. I can pipette now.

Lander Lab @landerlab.bsky.social · Mar 16

We’ve seen some good grids and some bad at the CSHL #CryoEM course, but I think we all agree that @miroastore.bsky.social should be awarded the 2025 “worst grid” award. But for someone who never held a pipette prior to this course he also deserves the “most improved” award - keep it up Miro!

Reposted by Miro Astore

Lander Lab @landerlab.bsky.social · Mar 16

Saturday night and the lab is in full swing as the participants of the CSHL #CryoEM course prepare for the final competition - solve the structure of a mystery protein in less than 4 hours.

Miro Astore @miroastore.bsky.social · Feb 17

Come work in a great place with great people. Loads of fun projects and ideas.

Sonya @sonyahanson.bsky.social · Feb 17

We are hiring a Flatiron Research Fellow in the Structural Molecular Biophysics team! Experience with cryoEM and/or membrane protein simulations are a plus! Happy to chat in person with anyone at #bps2025 ! apply.interfolio.com/160227

Apply - Interfolio {{$ctrl.$state.data.pageTitle}} - Apply - Interfolio

apply.interfolio.com

Reposted by Miro Astore

RCSB Protein Data Bank @rcsbpdb.bsky.social · Feb 5

Join leaders from CCDC, ICDD, ICSD, and RCSB PDB on February 12 to learn how data management impacts future research
register.gotowebinar...