Lightnews — Scholar-powered news

Reposted by Lander Lab

Jan-Hannes Schaefer @jhschaef.bsky.social · 3d

Helical Reconstruction of Amyloids in cryoSPARC?
We present guidelines, limitations & future perspectives for processing amyloids in cryoSPARC @structurabio.bsky.social.
Great collaboration of the Kelly Lab and @landerlab.bsky.social #cryoEM #amyloid www.biorxiv.org/content/10.1...

3 14 41

Lander Lab @landerlab.bsky.social · 25d

So happy to see these developments! Particularly the structure of the 29 kDa heterodimer of hemoglobin! Well done Oli & co! Of course can’t overlook the importance of sample prep and data collection - credit to Mark Herzik and Mengyu Wu

Oli Clarke @olibclarke.bsky.social · 26d

This one is a bit of a departure from the usual and definitely a work in progress!

We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.

Read on for details... 1/x

1 4 27

Reposted by Lander Lab

Jan-Hannes Schaefer @jhschaef.bsky.social · Aug 1

Check out our new preprint from the @landerlab.bsky.social, Stagg-Lab and Cianfrocco-Lab: CryoSift - An accessible and automated CNN-driven tool for cryo-EM 2D class selection www.biorxiv.org/content/10.1...
Happy testing: cryosift.org #cryoEM #CNN

CryoSift – An accessible and automated CNN-driven
tool for cryo-EM 2D class selection

1 7 17

Reposted by Lander Lab

Jan-Hannes Schaefer @jhschaef.bsky.social · Aug 14

Thanks for highlighting this great story! @landerlab.bsky.social and I wrote a PREreview about the preprint: prereview.org/reviews/1581...

PREreview of “High-resolution cryo-EM structures of small protein–ligand complexes near the theoretical size limit”

Authored by Jan-Hannes Schäfer and Gabriel C. Lander

prereview.org

1 3 11

Reposted by Lander Lab

Jan-Hannes Schaefer @jhschaef.bsky.social · Jun 23

Exited to share my first PostDoc contribution! Like Magellan explored the globe, you can explore your cryoEM data with Magellon. Congrats to all involved. Scott Stagg Lab & Michael Cianfrocco Lab @landerlab.bsky.social www.biorxiv.org/content/10.1... 🌎❄️🔬

11 22

Reposted by Lander Lab

BioTechniques @biotechniques.bsky.social · May 8

In this feature, Gabe Lander (@landerlab.bsky.social) and Adrian Koh discuss how they are working to improve cryo-EM technology >>> www.biotechniques.com/proteomics/s...

@scripps.edu 🧪

Seeking structure: an insight into cryo-EM, structural biology and drug development

Gabe Lander and Adrian Koh talk all things cryo-EM and its pivotal role in structural biology research in this feature.

www.biotechniques.com

1 4

Reposted by Lander Lab

Danielle Grotjahn @nanigrotjahn.bsky.social · May 1

🚨New preprint!🚨 #teamtomo
We expanded Surface Morphometrics to quantify membrane thickness from cryo-ET—revealing local variation across organelles.

Led by the lab’s first grad student, @mmedina300kv.bsky.social (defending Monday! 🍾) w/ @attychang.bsky.social @hamid13r.bsky.social & @tomo.science

bioRxivpreprint @biorxivpreprint.bsky.social · May 1

Surface morphometrics reveals local membrane thickness variation in organellar subcompartments https://www.biorxiv.org/content/10.1101/2025.04.30.651574v1

5 31 75

Lander Lab @landerlab.bsky.social · May 1

Our latest review is out in COSB! Led by Zeba Rizvi @zrizvi.bsky.social,‬ we explore how cryo-EM has brought new insights to Cullin RING ligases - from conformations to allosteric control, cryo-EM has massively improved our understanding of these dynamic molecular machines. tinyurl.com/y4sza8dh

1 22

Lander Lab @landerlab.bsky.social · Apr 23

3

Lander Lab @landerlab.bsky.social · Apr 21

~110 Å from side to side. We don’t see any particles that resemble the p97 dodecamer though, surely that orientation would appear in negative stain.

1

Lander Lab @landerlab.bsky.social · Apr 21

Social media has been great in helping us identify contaminants or cofactors in the past - Any ideas what this complex might be in our HEK293 FLAG pulldown? (We should put together a repository of common contaminants in different cell types)

zrizvi.bsky.social @zrizvi.bsky.social · Apr 21

First post here- Hello Bluesky!
Quick science query: These 2D classes are from negative stain of
a FLAG pulldown (HEK cell eluate). Does this look like a real complex or a common contaminant? Would live to hear from anyone who’s worked on similar prep!
#negativestainEM #pulldown #HEKcells

4 3 7

Reposted by Lander Lab

Lauren Anderson @lauanderson.bsky.social · Mar 23

Finally recovered from 2 weeks of #cryoEM What a blast! After 3 times TA-ing for CSHL, I can’t begin to describe the joy it has brought me to teach and learn at this amazing course. Thanks to @jencash.bsky.social @landerlab.bsky.social @matthijnvos.bsky.social et al. for bringing this to life!

1 10

Reposted by Lander Lab

Eric Topol @erictopol.bsky.social · Mar 23

A pie graph worth keeping in mind as the NIH budget plummets jamanetwork.com/journals/jam... for 356 new FDA drugs approved

62 1.7K 4K

Reposted by Lander Lab

Jan-Hannes Schaefer @jhschaef.bsky.social · Mar 22

Read of the week. Is this future of freezing cryo-EM grids - Foam Film Vitrification without blotting? Congrats to Russo and Löwe labs!

bioRxiv Biophysics @biorxiv-biophys.bsky.social · Mar 12

Foam film vitrification for cryo-EM https://www.biorxiv.org/content/10.1101/2025.03.10.642366v1

3 9

Lander Lab @landerlab.bsky.social · Mar 21

Big congrats to Dr. Kelly Chen on completing her PhD! She wrangled human ClpXP into submission with CryoEM, and now she's off to tackle new challenges at Genentech. We couldn’t be more proud of all she’s accomplished, and we all wish her all the best in this next chapter of her scientific journey.

27

Lander Lab @landerlab.bsky.social · Mar 19

That’s a wrap on the 2025 Cold Spring Harbor CryoEM course! Huge thanks to the amazing students for your enthusiasm, the TAs for your dedication, and the invited lecturers for sharing your expertise. Keep pushing the boundaries of single particle EM - excited to see what you all accomplish next!

4 25

Lander Lab @landerlab.bsky.social · Mar 19

Congrats to Group 1, winners of the 2025 CSHL #CryoEM course! They reconstructed a 1.9 A structure from 100 images, the built and refined an outstanding atomic model with great validation metrics. They are the recipients of the new Cryo Champion Chalice, adorned with $2,467 worth of used autogrids!

1 25

Lander Lab @landerlab.bsky.social · Mar 18

CSHL 2025 #CryoEM course update - final hour of the competition and the teams are refining their atomic models. Amazing what these students have done with 100 micrographs in just a few hours!

2 6

Lander Lab @landerlab.bsky.social · Mar 18

It’s the last full day of the CSHL #CryoEM course! Last leg of the competition - solve the structure of a mystery protein and generate an atomic model with a complete Table 1 (sequence not provided). Students are learning that ChatGPT isn’t much help when trying to identify symmetry of a structure…

4 18

Lander Lab @landerlab.bsky.social · Mar 18

Brace yourselves, Matthijn Vos @matthijnvos.bsky.social has arrived! EM expert & head of the NanoImaging Core at the Institut Pasteur, he's known for his razor-sharp critiques and brutal honesty. But beneath the grumpiness? A brilliant scientist and an invaluable colleague. Welcome, Matthijn!

1 9

Lander Lab @landerlab.bsky.social · Mar 17

Happy St. Patrick’s Day from the CSHL #cryoEM course! Data collection day for the final competition, but it looks like some mischievous leprechauns got into the microscope and messed up the autoloader… A tough but valuable lesson for the students: sometimes your resolution is limited by bad luck…

1 8

Lander Lab @landerlab.bsky.social · Mar 17

Day 11 of the CSHL #CryoEM course: depositing data and new developments at the EMDB with Jack Turner, @jencash.bsky.social showed how to make graphene oxide grids, and learned how to make local resolution maps. Competitive data collection starts tomorrow!

1 5

Lander Lab @landerlab.bsky.social · Mar 16

We’ve seen some good grids and some bad at the CSHL #CryoEM course, but I think we all agree that @miroastore.bsky.social should be awarded the 2025 “worst grid” award. But for someone who never held a pipette prior to this course he also deserves the “most improved” award - keep it up Miro!

1 2 22

Lander Lab @landerlab.bsky.social · Mar 16

Saturday night and the lab is in full swing as the participants of the CSHL #CryoEM course prepare for the final competition - solve the structure of a mystery protein in less than 4 hours.

1 3 29

Lander Lab @landerlab.bsky.social · Mar 14

Outstanding lecture on EM validation by Oli Clarke @olibclarke.bsky.social this morning at the CSHL #cryoEM course.

2 16