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Reposted by Maria Letizia Di Martino

Zeynep Baharoglu @zeynepbaharoglu.bsky.social · Sep 6

So excited our antibiotic potentiation story is out 🤩 Led by the extraordinary @manonlang.bsky.social with @fox-science.bsky.social & @amazeld.bsky.social +amazing collaborators @immunobladder.bsky.social @imaneelmeouche.bsky.social 🦠 We believe it can make a difference in #AMR infections!

Uridine as a potentiator of aminoglycosides through activation of carbohydrate transporters

Uridine boosts aminoglycoside treatment efficiency against antibiotic-susceptible as well as antibiotic-resistant E. coli strains.

www.science.org

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Reposted by Maria Letizia Di Martino

SellinLab @sellinlab.bsky.social · Aug 24

Mast cell subsets respond uniquely to Salmonella infection. Read all about it in our latest paper in EJI. Another chapter in our deciphering of mast cell interactions with enterobacteria, driven by Chris von Beek.

onlinelibrary.wiley.com/doi/10.1002/...

Mast Cell Phenotypic Heterogeneity Impacts the Interplay with Pathogenic Salmonella Typhimurium Bacteria

Mast cell phenotypic features influence anti-bacterial response. Connective tissue-like mast cells sense Salmonella through combined Toll-like-receptor (TLR) signaling and recognition of Type-III-sec....

onlinelibrary.wiley.com

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Reposted by Maria Letizia Di Martino

SellinLab @sellinlab.bsky.social · Aug 5

Such a rewarding collab it has been with our colleagues at #Novavax to dissect the MoA of their potent Matrix-M adjuvant. This project has really put the imaging skills of @erixoneriksson.bsky.social to the test. The fruits of this joint labour out now in NPJ Vaccines

www.nature.com/articles/s41...

Matrix-M adjuvant triggers inflammasome activation and enables antigen cross-presentation through induction of lysosomal membrane permeabilization - npj Vaccines

npj Vaccines - Matrix-M adjuvant triggers inflammasome activation and enables antigen cross-presentation through induction of lysosomal membrane permeabilization

www.nature.com

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Reposted by Maria Letizia Di Martino

Zeynep Baharoglu @zeynepbaharoglu.bsky.social · Aug 15

🦠 Launching a bimonthly digest on RNA modifications and related topics in bacteria!🤩 First issue: Summer 2025. Shared here + by email, future ones will be quicker reads! 😅 #rnasky #microsky #ribosome Subscribe and share if you are interested! rnamodifupdates.substack.com/p/rna-modifi...

RNA modifications digest Summer 2025

Bimonthly newsletter: RNA modifications and related topics, mostly in bacteria. Summer 2025, first issue!

rnamodifupdates.substack.com

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Reposted by Maria Letizia Di Martino

Nature Microbiology @natmicrobiol.nature.com · Aug 1

#NatMicroPicks

Decoding Shigella’s Virulence Blueprint 🦠🧬

Combining organoid infection models with genome-wide mutagenesis allows mapping the full set of Shigella genes required for human gut colonization

www.nature.com/articles/s41...

@sellinlab.bsky.social @natgenet.nature.com

A scalable gut epithelial organoid model reveals the genome-wide colonization landscape of a human-adapted pathogen - Nature Genetics

A genome-wide screen using human gut epithelial organoids combined with transposon-directed insertion sequencing identifies over 100 Shigella flexneri genes required for epithelial colonization.

www.nature.com

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Reposted by Maria Letizia Di Martino

Research & Innovation | University of Toronto Mississauga @utm-research.bsky.social · Jul 15

Could lab-grown mini-organs help scientists research a range of infections? A new study published in Nature Genetics shows how researchers have mapped how the Shigella bacterium infects the human gut.

Read our interview with study co-author — and UTM Biology prof — Lars Barquist at uoft.me/bI7.

Prof. Lars Barquist. Story title: One the Map: Experimental Research Uses Cultured Mini-Organs to Study Infections

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Maria Letizia Di Martino @mldm.bsky.social · Jun 24

Excited to be at #EESMicrobiology this week presenting our latest work on #Shigella gut #organoid infection screen from our recent Nature Genetics paper (see below)!
Stop by poster 109 or just look for me, happy to chat and connect!
#Microbiology #Shigella #Organoids #TraDIS

Nature Genetics @natgenet.nature.com · Jun 23

📢ONLINE @natgenet.nature.com

📰A scalable gut epithelial organoid model reveals the genome-wide colonization landscape of a human-adapted pathogen.

By @mldm.bsky.social, @lbarquist.bsky.social, Mikael E. Sellin and colleagues.

⬇️

www.nature.com/articles/s41...

A scalable gut epithelial organoid model reveals the genome-wide colonization landscape of a human-adapted pathogen - Nature Genetics

A genome-wide screen using human gut epithelial organoids combined with transposon-directed insertion sequencing identifies over 100 Shigella flexneri genes required for epithelial colonization.

www.nature.com

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Reposted by Maria Letizia Di Martino

bioRxiv Microbiology @biorxiv-microbiol.bsky.social · Jun 17

The pcnB gene sustains Shigella flexneri virulence https://www.biorxiv.org/content/10.1101/2025.06.16.659909v1

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Reposted by Maria Letizia Di Martino

Nature Portfolio @natureportfolio.nature.com · Jun 17

A study in Nature Genetics establishes the comprehensive genome-wide map of Shigella genes required to infect human intestinal epithelium. go.nature.com/4e7fdKp 🧬 🧪

This is figure 2, which shows a genome-wide map of the Shigella geneset required to invade the human enteroid infection model.

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Maria Letizia Di Martino @mldm.bsky.social · Jun 17

Huge congratulations to Thibault Frisch for leading this work to completion with remarkable insight and drive, and thanks to all co-authors for making it happen! @sellinlab.bsky.social, @lbarquist.bsky.social, @andrenmateus.bsky.social, @holmqvist-lab.bsky.social

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Maria Letizia Di Martino @mldm.bsky.social · Jun 17

pcnB (poly-A polymerase I) sustains replication of the Shigella virulence plasmid at levels optimal for virulence and boosts intraepithelial expansion in intestinal organoid monolayers.

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Maria Letizia Di Martino @mldm.bsky.social · Jun 17

New preprint out: www.biorxiv.org/content/10.1...
We followed up on our recent Nature Genetics paper mapping Shigella colonization in intestinal organoids with a deep dive into one of the screen hits: pcnB.

#Shigella #virulence #microsky

www.biorxiv.org

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Reposted by Maria Letizia Di Martino

SellinLab @sellinlab.bsky.social · Jun 13

Thanks @sydneylmiles.bsky.social and @sergemostowylab.bsky.social for writing such a sharp and thoughtful editorial, which covers all the main points and potential caveats of our study published yesterday.

www.nature.com/articles/s41...

Organoid models advance dysentery control - Nature Genetics

Shigella is a major human pathogen with a stark lack of pipelines linking genome content to clinical pathogenesis. An innovative study using large-scale organoid models, combined with genome-wide muta...

www.nature.com

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Maria Letizia Di Martino @mldm.bsky.social · Jun 13

Thank you so much!

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Reposted by Maria Letizia Di Martino

SellinLab @sellinlab.bsky.social · Jun 12

Our latest work combines human gut epithelial #organoid culture with experimental #Shigella #infections, transposon mutagenesis, and statistical modeling. This has led to the mapping of the comprehensive geneset that drives Shigella epithelial colonization.

Out @natgenet.nature.com:

rdcu.be/eqGgf

A scalable gut epithelial organoid model reveals the genome-wide colonization landscape of a human-adapted pathogen

Nature Genetics - A genome-wide screen using human gut epithelial organoids combined with transposon-directed insertion sequencing identifies over 100 Shigella flexneri genes required for...

rdcu.be

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Maria Letizia Di Martino @mldm.bsky.social · Jun 12

Stay tuned for a follow up story coming out soon!

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Reposted by Maria Letizia Di Martino

Lars Barquist @lbarquist.bsky.social · Jun 12

Very excited about our latest combining advanced cell culture, transposon-insertion sequencing, and statistical modeling to map Shigella virulence determinants in organoid models. Great collaboration w/ @mldm.bsky.social @sellinlab.bsky.social in @natgenet.nature.com

www.nature.com/articles/s41...

A scalable gut epithelial organoid model reveals the genome-wide colonization landscape of a human-adapted pathogen - Nature Genetics

A genome-wide screen using human gut epithelial organoids combined with transposon-directed insertion sequencing identifies over 100 Shigella flexneri genes required for epithelial colonization.

www.nature.com

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Reposted by Maria Letizia Di Martino

SciLifeLab @scilifelab.se · Jun 12

“we used mini organs, specifically mini-intestines, and infected them with a dangerous bacterium called Shigella” says @mldm.bsky.social‬

@sellinlab.bsky.social‬ (SciLifeLab & @uu.se) shows that cultured mini-organs can uncover how pathogens cause disease

youtu.be/xG_66ZGd_fk

Lab-grown mini-organs help uncover how pathogens cause disease

YouTube video by SciLifeLab

youtu.be

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Reposted by Maria Letizia Di Martino

Helmholtz Institute Würzburg @helmholtz-hiri.bsky.social · Jun 12

Thanks to lab-grown miniature intestines, researchers at #HIRI & Uppsala University have mapped how Shigella bacteria infect the human gut. This @natgenet.nature.com study opens the door to using cultured mini-organs to investigate a wide range of infections. 📍🦠
www.nature.com/articles/s41...

A scalable gut epithelial organoid model reveals the genome-wide colonization landscape of a human-adapted pathogen - Nature Genetics

A genome-wide screen using human gut epithelial organoids combined with transposon-directed insertion sequencing identifies over 100 Shigella flexneri genes required for epithelial colonization.

www.nature.com

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Maria Letizia Di Martino @mldm.bsky.social · Jun 12

Finally thanks @sydneylmiles.bsky.social and @sergemostowylab.bsky.social for for highlighting our work with this cool editorial: www.nature.com/articles/s41...

Organoid models advance dysentery control - Nature Genetics

Shigella is a major human pathogen with a stark lack of pipelines linking genome content to clinical pathogenesis. An innovative study using large-scale organoid models, combined with genome-wide muta...

www.nature.com

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Maria Letizia Di Martino @mldm.bsky.social · Jun 12

Deeply grateful to @sellinlab.bsky.social for the unwavering support! And special thanks to our lab members Anjeela Bhetwal, @erixoneriksson.bsky.social, Ana Lopes, Angelika Ntokaki, and Martina Pasqua for their contributions. Also grateful to @andrenmateus.bsky.social and our clinical collaborators

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Maria Letizia Di Martino @mldm.bsky.social · Jun 12

This study was co-led with our outstanding collaborators @lbarquist.bsky.social and Laura Jenniches — whose computational expertise was key to making this possible.

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Maria Letizia Di Martino @mldm.bsky.social · Jun 12

One of the most exciting insights: Shigella uses tRNA-modifying enzymes and codon usage bias to fine-tune its virulence!

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Maria Letizia Di Martino @mldm.bsky.social · Jun 12

Thrilled to share our work is finally out at @natgenet.nature.com ! rdcu.be/eqGgf
Combining a scalable human gut organoid model with TraDIS and statistical modeling, we mapped how Shigella colonizes the intestine at genome-wide resolution.

A scalable gut epithelial organoid model reveals the genome-wide colonization landscape of a human-adapted pathogen

Nature Genetics - A genome-wide screen using human gut epithelial organoids combined with transposon-directed insertion sequencing identifies over 100 Shigella flexneri genes required for...

rdcu.be

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Reposted by Maria Letizia Di Martino

SellinLab @sellinlab.bsky.social · May 30

Out now @mbio.bsky.social !: We provide a time-resolved map comparing #Salmonella and #Shigella epithelial colonization strategies in human #enteroids and #colonoids. Connecting the dots among decades of molecular findings reg. underlying virulence factors.

journals.asm.org/doi/10.1128/...

Determinants of divergent Salmonella and Shigella epithelial colonization strategies resolved in human enteroids and colonoids | mBio

Pathogenic bacteria employ partially overlapping sets of virulence factor functions to colonize host epithelia but can differ markedly in their pathogenesis and disease kinetics. This work combines ba...

journals.asm.org

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