@neilblackledge.bsky.social
Reposted
Excited to share our new preprint from the Klose lab, where we investigate how SET1/MLL complexes shape H3K4me3 and regulate transcription in mouse ES cells. www.biorxiv.org/content/10.6...
SET1/MLL complexes control transcription independently of H3K4me3
Histone H3 lysine 4 trimethylation (H3K4me3) at gene promoters is thought to play a central role in gene transcription. H3K4 methylation is deposited by the SET1 (A/B) and MLL (1-4) multi-protein comp...
www.biorxiv.org
December 11, 2025 at 4:07 PM
Excited to share our new preprint from the Klose lab, where we investigate how SET1/MLL complexes shape H3K4me3 and regulate transcription in mouse ES cells. www.biorxiv.org/content/10.6...
Reposted
An early Christmas present for those interested in chromatin and transcription! Fantastic work from @au-ho-yu.bsky.social and @aleksszczurek.bsky.social . Thanks to Inge and Michiel for their help. Please repost!
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
SET1/MLL complexes control transcription independently of H3K4me3
Histone H3 lysine 4 trimethylation (H3K4me3) at gene promoters is thought to play a central role in gene transcription. H3K4 methylation is deposited by the SET1 (A/B) and MLL (1-4) multi-protein comp...
www.biorxiv.org
December 11, 2025 at 6:09 AM
An early Christmas present for those interested in chromatin and transcription! Fantastic work from @au-ho-yu.bsky.social and @aleksszczurek.bsky.social . Thanks to Inge and Michiel for their help. Please repost!
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Reposted
We have had multiple discussions about why people have not previously observed the very dramatic reduction in transcription upon PNUTS depletion. So I looked at our own TT-seq data and analysed it without including the spike-in. The numbers speak for themselves! Spike-in calibration is important!
December 12, 2024 at 10:50 AM
We have had multiple discussions about why people have not previously observed the very dramatic reduction in transcription upon PNUTS depletion. So I looked at our own TT-seq data and analysed it without including the spike-in. The numbers speak for themselves! Spike-in calibration is important!
Reposted
We don’t think the impact factor is a good thing. But this is not the fight we want right now. We are trying something amazing and it’s working. We don’t want authors or other journals to fear trying it. Clarivate know they have this control and are using it to squash innovation. It is bullying.
I have been trying to figure out whether eLife's position is that they approve or disapprove of the Clarivate decision to no longer give them an impact factor.
This makes it seem like they disapprove of it, although it doesn't exactly say that.
This makes it seem like they disapprove of it, although it doesn't exactly say that.
As a long-term signatory of the Declaration on Research Assessment, we thank DORA for this supportive message. There has been an ongoing move away from journal-level metrics; we hope this will only accelerate now.
November 30, 2024 at 7:10 AM
We don’t think the impact factor is a good thing. But this is not the fight we want right now. We are trying something amazing and it’s working. We don’t want authors or other journals to fear trying it. Clarivate know they have this control and are using it to squash innovation. It is bullying.
Reposted
Latest from the Klose lab: we discover a new role for the PNUTS-PP1 phosphatase complex in regulation of PolII pause release. www.cell.com/molecular-ce...
The PNUTS phosphatase complex controls transcription pause release
Kelley et al. discover that the PNUTS phosphatase complex plays an essential role
in gene transcription by controlling RNA polymerase II pause release. PNUTS achieves
this through its TND, which recog...
www.cell.com
November 26, 2024 at 10:08 PM
Latest from the Klose lab: we discover a new role for the PNUTS-PP1 phosphatase complex in regulation of PolII pause release. www.cell.com/molecular-ce...
Fantastic to see the latest story from the Klose lab out in Molecular Cell today! Thoroughly enjoyed being part of a team effort spearheaded by @jesskelley.bsky.social and @edimitrova.bsky.social! A project that briefly dragged us away from CpG islands before leading us back to the 5’ end of genes!
Very excited that our paper describing a critical role for the PNUTS-PP1 phosphatase complex in transcription pause release is up online! This was a massive team effort with @edimitrova.bsky.social and the rest of the @robklose.bsky.social lab www.cell.com/molecular-ce... A short thread: (1/8)
The PNUTS phosphatase complex controls transcription pause release
Kelley et al. discover that the PNUTS phosphatase complex plays an essential role
in gene transcription by controlling RNA polymerase II pause release. PNUTS achieves
this through its TND, which recog...
www.cell.com
November 26, 2024 at 5:06 PM
Fantastic to see the latest story from the Klose lab out in Molecular Cell today! Thoroughly enjoyed being part of a team effort spearheaded by @jesskelley.bsky.social and @edimitrova.bsky.social! A project that briefly dragged us away from CpG islands before leading us back to the 5’ end of genes!