Rob Klose
@robklose.bsky.social
Professor of Genetics
Department of Biochemistry
University of Oxford
kloselab.co.uk
Department of Biochemistry
University of Oxford
kloselab.co.uk
Reposted by Rob Klose
Our attempt to give multinucleate cells the spotlight they deserve (seriously, they’re everywhere), led by the fearless @mrosjac.bsky.social and with @Markus Ganter
doi.org/10.32942/X2M...
We’d love your feedback while this goes through the peer review process!
#MicroEvoSky
#ProtistsonSky 🧪🌏
doi.org/10.32942/X2M...
We’d love your feedback while this goes through the peer review process!
#MicroEvoSky
#ProtistsonSky 🧪🌏
November 26, 2025 at 2:05 PM
Our attempt to give multinucleate cells the spotlight they deserve (seriously, they’re everywhere), led by the fearless @mrosjac.bsky.social and with @Markus Ganter
doi.org/10.32942/X2M...
We’d love your feedback while this goes through the peer review process!
#MicroEvoSky
#ProtistsonSky 🧪🌏
doi.org/10.32942/X2M...
We’d love your feedback while this goes through the peer review process!
#MicroEvoSky
#ProtistsonSky 🧪🌏
Reposted by Rob Klose
Single-Molecule DNA Footprinting and Transcription Imaging Reveal the Molecular Mechanisms of Promoter Dynamics https://www.biorxiv.org/content/10.1101/2025.11.26.690466v1
November 27, 2025 at 3:19 AM
Single-Molecule DNA Footprinting and Transcription Imaging Reveal the Molecular Mechanisms of Promoter Dynamics https://www.biorxiv.org/content/10.1101/2025.11.26.690466v1
Reposted by Rob Klose
We seem to be in the golden age of centromere biology
A new and fascinating story from @bencarty.bsky.social and the group, with crucial help from the teams of @naltemose.bsky.social, Simona Giunta, and @dfachinetti.bsky.social. Many thanks to all for a fantastic collaboration.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
November 25, 2025 at 12:41 PM
We seem to be in the golden age of centromere biology
Reposted by Rob Klose
We are recruiting a postdoctoral fellow to study mechanisms of DNA replication stress using single-molecule imaging tools. If you are excited about microscopy, replisome dynamics, and genome stability, we’d love to hear from you.
Application deadline: 24 December
www.crick.ac.uk/careers-stud...
Application deadline: 24 December
www.crick.ac.uk/careers-stud...
Postdoctoral Fellow - H Yardimci Lab
www.crick.ac.uk
November 25, 2025 at 11:55 AM
We are recruiting a postdoctoral fellow to study mechanisms of DNA replication stress using single-molecule imaging tools. If you are excited about microscopy, replisome dynamics, and genome stability, we’d love to hear from you.
Application deadline: 24 December
www.crick.ac.uk/careers-stud...
Application deadline: 24 December
www.crick.ac.uk/careers-stud...
Reposted by Rob Klose
The Richard and Susan Smith Family Foundation, which supports our work, has produced a short film about our research:
November 26, 2025 at 1:22 AM
The Richard and Susan Smith Family Foundation, which supports our work, has produced a short film about our research:
Reposted by Rob Klose
And now we have Arabidopsis plants with 8 chromosomes instead of 10 and no obvious phenotypic differences, this week in @science.org
#PlantScience
Paper here: www.science.org/doi/10.1126/...
Perspective here:
www.science.org/doi/10.1126/...
#PlantScience
Paper here: www.science.org/doi/10.1126/...
Perspective here:
www.science.org/doi/10.1126/...
CRISPR-Cas–mediated heritable chromosome fusions in Arabidopsis
The genome of Arabidopsis thaliana consists of 10 chromosomes. By inducing CRISPR-Cas–mediated breaks at subcentromeric and subtelomeric sequences, we fused entire chromosome arms, obtaining two eight...
www.science.org
November 21, 2025 at 10:20 AM
And now we have Arabidopsis plants with 8 chromosomes instead of 10 and no obvious phenotypic differences, this week in @science.org
#PlantScience
Paper here: www.science.org/doi/10.1126/...
Perspective here:
www.science.org/doi/10.1126/...
#PlantScience
Paper here: www.science.org/doi/10.1126/...
Perspective here:
www.science.org/doi/10.1126/...
Reposted by Rob Klose
Check out that spindle 🤩🔥
At 63C, most eukaryotic cells would be busy exploding noisily, forget even trying to divide.
The microbial universe never ceases to astound 😍
Thanks for letting us be a tiny part of this! #ExpandThemAll
At 63C, most eukaryotic cells would be busy exploding noisily, forget even trying to divide.
The microbial universe never ceases to astound 😍
Thanks for letting us be a tiny part of this! #ExpandThemAll
While 𝘝. 𝘷𝘦𝘳𝘮𝘪𝘧𝘰𝘳𝘮𝘪𝘴 max temp is around 45°C, we found 𝘐. 𝘤𝘢𝘴𝘤𝘢𝘥𝘦𝘯𝘴𝘪𝘴 grows up to 63°C with optimal growth at 55-57°C🥵. We showed cellular replication via Ultrastructure Expansion Microscopy at 63°C. The previous limit for euks is 60°C!
November 25, 2025 at 8:30 PM
Check out that spindle 🤩🔥
At 63C, most eukaryotic cells would be busy exploding noisily, forget even trying to divide.
The microbial universe never ceases to astound 😍
Thanks for letting us be a tiny part of this! #ExpandThemAll
At 63C, most eukaryotic cells would be busy exploding noisily, forget even trying to divide.
The microbial universe never ceases to astound 😍
Thanks for letting us be a tiny part of this! #ExpandThemAll
Reposted by Rob Klose
So happy to announce our new preprint, “A geothermal amoeba sets a new upper temperature limit for eukaryotes.” We cultured a novel amoeba from Lassen Volcanic NP (CA, USA) that divides at 63°C (145°F) 🔥 - a new record for euk growth!
#protistsonsky 🧵
#protistsonsky 🧵
November 25, 2025 at 8:41 PM
So happy to announce our new preprint, “A geothermal amoeba sets a new upper temperature limit for eukaryotes.” We cultured a novel amoeba from Lassen Volcanic NP (CA, USA) that divides at 63°C (145°F) 🔥 - a new record for euk growth!
#protistsonsky 🧵
#protistsonsky 🧵
Reposted by Rob Klose
A new and fascinating story from @bencarty.bsky.social and the group, with crucial help from the teams of @naltemose.bsky.social, Simona Giunta, and @dfachinetti.bsky.social. Many thanks to all for a fantastic collaboration.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
November 25, 2025 at 12:16 PM
A new and fascinating story from @bencarty.bsky.social and the group, with crucial help from the teams of @naltemose.bsky.social, Simona Giunta, and @dfachinetti.bsky.social. Many thanks to all for a fantastic collaboration.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Rob Klose
Project 2: Use human ES cells to determine exactly how DNMT3B mutations cause ICF1 syndrome. Collaboration with @hannahlong.bsky.social:
institute-genetics-cancer.ed.ac.uk/igc-graduate...
Deadline 11th January 2026
This scheme is flexible if you are interested in other projects.
🧵 3/4
institute-genetics-cancer.ed.ac.uk/igc-graduate...
Deadline 11th January 2026
This scheme is flexible if you are interested in other projects.
🧵 3/4
November 25, 2025 at 9:15 AM
Project 2: Use human ES cells to determine exactly how DNMT3B mutations cause ICF1 syndrome. Collaboration with @hannahlong.bsky.social:
institute-genetics-cancer.ed.ac.uk/igc-graduate...
Deadline 11th January 2026
This scheme is flexible if you are interested in other projects.
🧵 3/4
institute-genetics-cancer.ed.ac.uk/igc-graduate...
Deadline 11th January 2026
This scheme is flexible if you are interested in other projects.
🧵 3/4
Reposted by Rob Klose
🚨 Multiple #PhD opportunities for lab or computational applicants. Do you want to understand the molecular basis of #epigenetic dysfunction in human disease?
Come work with our group at the fantastic, collaborative @uoe-igc.bsky.social in a beautiful, liveable city.
🧵 1/4
Please repost 🙏
Come work with our group at the fantastic, collaborative @uoe-igc.bsky.social in a beautiful, liveable city.
🧵 1/4
Please repost 🙏
November 25, 2025 at 9:15 AM
🚨 Multiple #PhD opportunities for lab or computational applicants. Do you want to understand the molecular basis of #epigenetic dysfunction in human disease?
Come work with our group at the fantastic, collaborative @uoe-igc.bsky.social in a beautiful, liveable city.
🧵 1/4
Please repost 🙏
Come work with our group at the fantastic, collaborative @uoe-igc.bsky.social in a beautiful, liveable city.
🧵 1/4
Please repost 🙏
Reposted by Rob Klose
Another great PhD opportunity for those interested in cancer!
🎓 We have a fully funded PhD opportunity in my lab and the Newcastle Cancer Drug Discovery Group to explore how oncogene-driven replication stress fuels Squamous Cell Carcinomas—and how to target it. #CancerResearch #PhD
📅 Apply by: 4 Dec 2025
🔗 Apply here:
www.findaphd.com/phds/project...
📅 Apply by: 4 Dec 2025
🔗 Apply here:
www.findaphd.com/phds/project...
MRC DiMeN Doctoral Training Partnership: Targeting oncogene-induced replication stress in squamous cell carcinoma at Newcastle University on FindAPhD.com
PhD Project - MRC DiMeN Doctoral Training Partnership: Targeting oncogene-induced replication stress in squamous cell carcinoma at Newcastle University, listed on FindAPhD.com
www.findaphd.com
November 25, 2025 at 11:18 AM
Another great PhD opportunity for those interested in cancer!
Reposted by Rob Klose
open postdoc call in our friendly neighborgroup with Esben Lorentzen. Higly recommed🌟:
www.au.dk/om/stillinge...
www.au.dk/om/stillinge...
Postdoctoral Position in Structural and mechanistic investigation of the ciliary trafficking machinery bound to cargo, Denmark - Ledig stilling på Aarhus Universitet
Ledig stilling ved Institut for Molekylærbiologi og Genetik - Proteinvidenskab, Aarhus Universitet
www.au.dk
November 24, 2025 at 8:13 AM
open postdoc call in our friendly neighborgroup with Esben Lorentzen. Higly recommed🌟:
www.au.dk/om/stillinge...
www.au.dk/om/stillinge...
Reposted by Rob Klose
The entrance to the 3-storey bike parking garage at Utrecht Central holding 12,500 bicycles. It’s free for the first 24 hours, linked to the station, and used by tens of thousands every day. This is what happens when a country treats bikes as real transport infrastructure, not an afterthought.
November 22, 2025 at 7:08 PM
The entrance to the 3-storey bike parking garage at Utrecht Central holding 12,500 bicycles. It’s free for the first 24 hours, linked to the station, and used by tens of thousands every day. This is what happens when a country treats bikes as real transport infrastructure, not an afterthought.
Reposted by Rob Klose
Last call! Applications for the @crg.eu International PhD Programme 2025 in beautiful Barcelona close next week! Join us and our inspiring colleagues at the CRG - an exceptional environment for discovery, creativity, and scientific growth.
Apply here: www.crg.eu/en/content/t...
Apply here: www.crg.eu/en/content/t...
November 22, 2025 at 1:36 PM
Last call! Applications for the @crg.eu International PhD Programme 2025 in beautiful Barcelona close next week! Join us and our inspiring colleagues at the CRG - an exceptional environment for discovery, creativity, and scientific growth.
Apply here: www.crg.eu/en/content/t...
Apply here: www.crg.eu/en/content/t...
Reposted by Rob Klose
How does messenger RNA (mRNA) get out of the nucleus to become a protein? Eukaryotic mRNA is packaged, exported, and then translated in the cytoplasm. But how do these steps work? And what are open questions? Check out our new review for our take: www.annualreviews.org/content/jour... (1/3)
November 21, 2025 at 5:37 PM
How does messenger RNA (mRNA) get out of the nucleus to become a protein? Eukaryotic mRNA is packaged, exported, and then translated in the cytoplasm. But how do these steps work? And what are open questions? Check out our new review for our take: www.annualreviews.org/content/jour... (1/3)
Reposted by Rob Klose
Yesterday’s lab lunch felt really special. We took a moment to celebrate new papers, promotions, fresh faces in the lab—and, most of all, friendship. Grateful for this team. We’re stronger than ever.
November 22, 2025 at 12:20 PM
Yesterday’s lab lunch felt really special. We took a moment to celebrate new papers, promotions, fresh faces in the lab—and, most of all, friendship. Grateful for this team. We’re stronger than ever.
Reposted by Rob Klose
Last few days to apply for this PhD project in our lab. Mix of biochemistry and structural biology, DNA replication and ubiquitin signalling. Get in touch if interested. Full application through official university portal. Deadline on 27th of November!
Fully-funded 4-years competitive PhD project in our lab (MIBTP-doctoral-training-programme. p97 unfoldase functions during DNA replication. Open to UK/international candidates. Please get in touch for informal enquiries. Deadline 27th of November. Please share.
warwick.ac.uk/fac/cross_fa...
warwick.ac.uk/fac/cross_fa...
Professor Aga Gambus
Professor Aga Gambus
warwick.ac.uk
November 22, 2025 at 8:44 AM
Last few days to apply for this PhD project in our lab. Mix of biochemistry and structural biology, DNA replication and ubiquitin signalling. Get in touch if interested. Full application through official university portal. Deadline on 27th of November!
Reposted by Rob Klose
The Brixen Summer School is back and is, as usual, building up a strong speaker list. Thank you @eupaproteomics.bsky.social @thermofishersci.bsky.social @biognosys.bsky.social @ionopticks.bsky.social @evosep.bsky.social for support!
November 21, 2025 at 9:54 AM
The Brixen Summer School is back and is, as usual, building up a strong speaker list. Thank you @eupaproteomics.bsky.social @thermofishersci.bsky.social @biognosys.bsky.social @ionopticks.bsky.social @evosep.bsky.social for support!
Reposted by Rob Klose
Very proud of our new paper! Great job @mweilert.bsky.social, our experimentalists and modeling collaborator Rosa Martinez-Corral. It was fun to see the story grow and get feedback from various experts. Thank you all!
We are pleased to announce a new preprint by @mlweilert.bsky.social: “Widespread low-affinity motifs enhance chromatin accessibility and regulatory potential in mESCs” (www.biorxiv.org/content/10.1...). See summary and longer recap below:
(TLDR; low-affinity motifs matter as pioneers!)
(TLDR; low-affinity motifs matter as pioneers!)
Widespread low-affinity motifs enhance chromatin accessibility and regulatory potential in mESCs
Low-affinity transcription factor (TF) motifs are an important element of the cis-regulatory code, yet they are notoriously difficult to map and mechanistically incompletely understood, limiting our a...
www.biorxiv.org
November 19, 2025 at 11:38 PM
Very proud of our new paper! Great job @mweilert.bsky.social, our experimentalists and modeling collaborator Rosa Martinez-Corral. It was fun to see the story grow and get feedback from various experts. Thank you all!
Reposted by Rob Klose
Dead Sperm Whale on Nantucket Is Too Big to Move, for Now
www.nytimes.com
November 20, 2025 at 9:46 PM
Reposted by Rob Klose
1/ I’m very excited to share my postdoc work with @kseskv.bsky.social, in collaboration with @oligriffith.bsky.social. We explore embryonic DNA methylation reprogramming in the fat-tailed dunnart, an Australian marsupial 🦘🌏 www.biorxiv.org/content/10.1...
www.biorxiv.org
November 12, 2025 at 2:57 AM
1/ I’m very excited to share my postdoc work with @kseskv.bsky.social, in collaboration with @oligriffith.bsky.social. We explore embryonic DNA methylation reprogramming in the fat-tailed dunnart, an Australian marsupial 🦘🌏 www.biorxiv.org/content/10.1...
Reposted by Rob Klose
To probe gene-scale chromatin physics, we built 96-mer (20 kb) arrays with defined histone marks. Combining single-molecule tracking, AFM imaging, and developing in vitro Hi-C, we saw how specific modifications dictate chromatin structure and dynamics. www.science.org/doi/10.1126/...
Gene-scale in vitro reconstitution reveals histone acetylation directly controls chromatin architecture
Reconstituting 20-kb chromatin shows that tuning acetylation alone reshapes its folding, dynamics, and contact domain formation.
www.science.org
November 20, 2025 at 7:46 AM
To probe gene-scale chromatin physics, we built 96-mer (20 kb) arrays with defined histone marks. Combining single-molecule tracking, AFM imaging, and developing in vitro Hi-C, we saw how specific modifications dictate chromatin structure and dynamics. www.science.org/doi/10.1126/...
Reposted by Rob Klose
piRNA-directed DNA methylation may not be a mammalian invention, but can already be found in the germline of axolotl salamanders
Donal O’Carroll and coworkers
www.embopress.org/doi/full/10....
Donal O’Carroll and coworkers
www.embopress.org/doi/full/10....
November 13, 2025 at 11:54 AM
piRNA-directed DNA methylation may not be a mammalian invention, but can already be found in the germline of axolotl salamanders
Donal O’Carroll and coworkers
www.embopress.org/doi/full/10....
Donal O’Carroll and coworkers
www.embopress.org/doi/full/10....