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Reposted by Nathan Fraikin

Géraldine Laloux @lalouxlab.bsky.social · 22d

🚨New paper out! #MicroSky
Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival.
We expanded its genetic toolbox:
🧬promoters to fine-tune expression
🧬IPTG-inducible system
🧬CRISPRi for rapid knockdown
bit.ly/46GUn2c
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Expanding the genetic toolbox of the obligate predatory bacterium Bdellovibrio bacteriovorus with inducible gene expression and CRISPR interference

Inducible gene expression and CRISPR interference-mediated depletion in the predatory bacterium Bdellovibrio bacteriovorus.

academic.oup.com

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Reposted by Nathan Fraikin

Agathe couturier @agathe-couturier.bsky.social · 21d

Exclusion systems, far from slowing down plasmid spread, are in fact essential to preserve host cell viability and thereby ensure the successful dissemination of conjugative plasmids and antibiotic resistance genes.

@nfrk92.bsky.social
@narjournal.bsky.social

academic.oup.com/nar/article/...

Exclusion systems preserve host cell homeostasis and fitness, ensuring successful dissemination of conjugative plasmids and associated resistance genes

Abstract. Plasmid conjugation is a major driver of antibiotic resistance dissemination in bacteria. In addition to genes required for transfer and maintena

academic.oup.com

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Reposted by Nathan Fraikin

Knut Drescher @knutdrescher.bsky.social · Apr 28

Biofilm architecture determines the dissemination of conjugative plasmids: We measured the spatiotemporal spread of plasmids in biofilms and found that plasmids only spread in some biofilm regions!

Collab with lab of @sbigot.bsky.social & Christian Lesterlin
www.pnas.org/doi/10.1073/...

Biofilm architecture determines the dissemination of conjugative plasmids | PNAS

Plasmid conjugation is a contact-dependent horizontal gene transfer mechanism that significantly contributes to the dissemination of antibiotic res...

www.pnas.org

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Nathan Fraikin @nfrk92.bsky.social · Apr 24

And a complete heresy to mix it in cocktails !

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Nathan Fraikin @nfrk92.bsky.social · Apr 24

www.addgene.org/browse/artic... (Available from Addgene @addgene.bsky.social )

Addgene: A palette of bright and photostable monomeric fluorescent proteins for bacterial time-lapse imaging

www.addgene.org

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Nathan Fraikin @nfrk92.bsky.social · Apr 23

Our set of new prokaryote-optimized fluorescent proteins is now published ! #microbiology #microsky #microscopy 🧫🦠🔬
www.science.org/doi/10.1126/...

A palette of bright and photostable monomeric fluorescent proteins for bacterial time-lapse imaging

Bright and photostable monomeric fluorescent proteins enable long-term time-lapse imaging in E. coli.

www.science.org

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Reposted by Nathan Fraikin

MMSB-Lyon @mmsb-lyon.bsky.social · Apr 23

MMSB - Molecular Microbiology and Structural Biochemistry @mmsb-lyon.bsky.social is now on Bluesky!
👉 Follow us to know about our last publications, prizes, job offers, seminars, events,...

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Nathan Fraikin @nfrk92.bsky.social · Apr 23

Something with 1-10 mM Mg2+

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Nathan Fraikin @nfrk92.bsky.social · Apr 9

WHAAAAAT, we do !!!

Reposted by Nathan Fraikin

Alvaro San Millan @sanmillan.bsky.social · Jan 24

New preprint from the lab!! Check it out!

Alicia Calvo-Villamañán @aliciapcv.bsky.social · Jan 24

🚨 New preprint! We know that plasmids are associated with very variable fitness costs in their different bacterial hosts. But, what is the contribution of each of the plasmid-genes in these host-specific effects? Study led by @jorgesastred.bsky.social, @sanmillan.bsky.social and myself! 1/14

Dissecting pOXA-48 fitness effects in clinical enterobacteria using plasmid-wide CRISPRi screens

Conjugative plasmids are the main vehicle for the spread of antimicrobial resistance (AMR) genes in clinical bacteria. AMR plasmids allow bacteria to survive antibiotic treatments, but they also produ...

www.biorxiv.org

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Reposted by Nathan Fraikin

Sarah Bigot @sbigot.bsky.social · Jan 25

New preprint from our Lesterlin Lab! Yannick Baffert and @nfrk92.bsky.social, in collaboration with @macerwan.bsky.social and @val-meve.bsky.social, shed light on the molecular mechanisms driving pOXA-48 plasmid maintenance and transfer. We reveal novel TA system, regulators, and T4SS components!

Genetic Determinants of pOXA-48 Plasmid Maintenance and Propagation in Escherichia coli

Conjugative plasmids are the main drivers of antibiotic resistance dissemination contributing to the emergence and extensive spread of multidrug resistance clinical bacterial pathogens. pOXA-48 plasmi...

www.biorxiv.org

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Nathan Fraikin @nfrk92.bsky.social · Dec 29

which recombination pathway and which species are we talking about ?

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Nathan Fraikin @nfrk92.bsky.social · Dec 10

fr.wiktionary.org/wiki/Raoulte...

a man in a lab coat is sitting at a desk with the words moi je suis une star des maladies infectieuses written below him

ALT: a man in a lab coat is sitting at a desk with the words moi je suis une star des maladies infectieuses written below him

media.tenor.com

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Nathan Fraikin @nfrk92.bsky.social · Dec 4

sesame street count von count is singing into a microphone while wearing a tuxedo

ALT: sesame street count von count is singing into a microphone while wearing a tuxedo

media.tenor.com

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Nathan Fraikin @nfrk92.bsky.social · Jan 15

A great paradigm shift for the proximal-distal expression gradient in (super)integrons. Bravo Paula, Jose & team !

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Reposted by Nathan Fraikin

Steve Diggle @digglelab.bsky.social · Dec 8

RIP Pierre. You were one of the best. Thank you for all you did for our field but more importantly, for me and all the people you helped. I will miss you. ❤️

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Nathan Fraikin @nfrk92.bsky.social · Nov 27

Gram-pos envelope is like a thick layer of homogenous porous material while gram-neg is more like a composite that keeps all kind of stuff out of the cell. My money's on gram-neg.

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Nathan Fraikin @nfrk92.bsky.social · Nov 27

weapons allowed ?

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